Marine20—the marine radiocarbon age calibration curve (0 – 55,000 cal BP)

T.J. Heaton is supported by a Leverhulme Trust Fellowship RF-2019-140\9, “Improving the Measurement of Time Using Radiocarbon”. M Butzin is supported by the German Federal Ministry of Education and Research (BMBF), as Research for Sustainability initiative (FONA); www.fona.de through the PalMod project (grant numbers: 01LP1505B, 01LP1919A). E. Bard is supported by EQUIPEX ASTER-CEREGE and ANR CARBOTRYDH. Meetings of the IntCal Marine Focus group have been supported by Collège de France.The concentration of radiocarbon (14C) differs between ocean and atmosphere. Radiocarbon determinations from samples which obtained their 14C in the marine environment therefore need a marine-specific calibration curve and cannot be calibrated directly against the atmospheric-based IntCal20 curve. This paper presents Marine20, an update to the internationally agreed marine radiocarbon age calibration curve that provides a non-polar global-average marine record of radiocarbon from 0–55 cal kBP and serves as a baseline for regional oceanic variation. Marine20 is intended for calibration of marine radiocarbon samples from non-polar regions; it is not suitable for calibration in polar regions where variability in sea ice extent, ocean upwelling and air-sea gas exchange may have caused larger changes to concentrations of marine radiocarbon. The Marine20 curve is based upon 500 simulations with an ocean/atmosphere/biosphere box-model of the global carbon cycle that has been forced by posterior realizations of our Northern Hemispheric atmospheric IntCal20 14C curve and reconstructed changes in CO2 obtained from ice core data. These forcings enable us to incorporate carbon cycle dynamics and temporal changes in the atmospheric 14C level. The box-model simulations of the global-average marine radiocarbon reservoir age are similar to those of a more complex three-dimensional ocean general circulation model. However, simplicity and speed of the box model allow us to use a Monte Carlo approach to rigorously propagate the uncertainty in both the historic concentration of atmospheric 14C and other key parameters of the carbon cycle through to our final Marine20 calibration curve. This robust propagation of uncertainty is fundamental to providing reliable precision for the radiocarbon age calibration of marine based samples. We make a first step towards deconvolving the contributions of different processes to the total uncertainty; discuss the main differences of Marine20 from the previous age calibration curve Marine13; and identify the limitations of our approach together with key areas for further work. The updated values for ΔR, the regional marine radiocarbon reservoir age corrections required to calibrate against Marine20, can be found at the data base http://calib.org/marine/.Publisher PDFPeer reviewe

Marine20—The Marine Radiocarbon Age Calibration Curve (0–55,000 cal BP)

The concentration of radiocarbon (14C) differs between ocean and atmosphere. Radiocarbon determinations from samples which obtained their 14C in the marine environment therefore need a marine-specific calibration curve and cannot be calibrated directly against the atmospheric-based IntCal20 curve. This paper presents Marine20, an update to the internationally agreed marine radiocarbon age calibration curve that provides a non-polar global-average marine record of radiocarbon from 0–55 cal kBP and serves as a baseline for regional oceanic variation. Marine20 is intended for calibration of marine radiocarbon samples from non-polar regions; it is not suitable for calibration in polar regions where variability in sea ice extent, ocean upwelling and air-sea gas exchange may have caused larger changes to concentrations of marine radiocarbon. The Marine20 curve is based upon 500 simulations with an ocean/atmosphere/biosphere box-model of the global carbon cycle that has been forced by posterior realizations of our Northern Hemispheric atmospheric IntCal20 14C curve and reconstructed changes in CO2 obtained from ice core data. These forcings enable us to incorporate carbon cycle dynamics and temporal changes in the atmospheric 14C level. The box-model simulations of the global-average marine radiocarbon reservoir age are similar to those of a more complex three-dimensional ocean general circulation model. However, simplicity and speed of the box model allow us to use a Monte Carlo approach to rigorously propagate the uncertainty in both the historic concentration of atmospheric 14C and other key parameters of the carbon cycle through to our final Marine20 calibration curve. This robust propagation of uncertainty is fundamental to providing reliable precision for the radiocarbon age calibration of marine based samples. We make a first step towards deconvolving the contributions of different processes to the total uncertainty; discuss the main differences of Marine20 from the previous age calibration curve Marine13; and identify the limitations of our approach together with key areas for further work. The updated values for ΔR, the regional marine radiocarbon reservoir age corrections required to calibrate against Marine20, can be found at the data bas

One origin for metallo-β-lactamase activity, or two? An investigation assessing a diverse set of reconstructed ancestral sequences based on a sample of phylogenetic trees

This work was supported by BBSRC (grant BB/F016778/1)Bacteria use metallo-β-lactamase enzymes to hydrolyse lactam rings found in many antibiotics, rendering them ineffective. Metallo-β-lactamase activity is thought to be polyphyletic, having arisen on more than one occasion within a single functionally diverse homologous superfamily. Since discovery of multiple origins of enzymatic activity conferring antibiotic resistance has broad implications for the continued clinical use of antibiotics, we test the hypothesis of polyphyly further; if lactamase function has arisen twice independently, the most recent common ancestor (MRCA) is not expected to possess lactam-hydrolysing activity. Two major problems present themselves. Firstly, even with a perfectly known phylogeny, ancestral sequence reconstruction is error prone. Secondly, the phylogeny is not known, and in fact reconstructing a single, unambiguous phylogeny for the superfamily has proven impossible. To obtain a more statistical view of the strength of evidence for or against MRCA lactamase function, we reconstructed a sample of 98 MRCAs of the metallo-β-lactamases, each based on a different tree in a bootstrap sample of reconstructed phylogenies. InterPro sequence signatures and homology modelling were then used to assess our sample of MRCAs for lactamase functionality. Only 5 % of these models conform to our criteria for metallo-β-lactamase functionality, suggesting that the ancestor was unlikely to have been a metallo-β-lactamase. On the other hand, given that ancestral proteins may have had metallo-β-lactamase functionality with variation in sequence and structural properties compared with extant enzymes, our criteria are conservative, estimating a lower bound of evidence for metallo-β-lactamase functionality but not an upper bound.Publisher PDFPeer reviewe

Atlantic Deep-water Response to the Early Pliocene Shoaling of the Central American Seaway

The early Pliocene shoaling of the Central American Seaway (CAS), ~4.7–4.2 million years ago (mega annum-Ma), is thought to have strengthened Atlantic Meridional Overturning Circulation (AMOC). The associated increase in northward flux of heat and moisture may have significantly influenced the evolution of Pliocene climate. While some evidence for the predicted increase in North Atlantic Deep Water (NADW) formation exists in the Caribbean and Western Atlantic, similar evidence is missing in the wider Atlantic. Here, we present stable carbon (δ13C) and oxygen (δ18O) isotope records from the Southeast Atlantic-a key region for monitoring the southern extent of NADW. Using these data, together with other δ13C and δ18O records from the Atlantic, we assess the impact of the early Pliocene CAS shoaling phase on deep-water circulation. We find that NADW formation was vigorous prior to 4.7 Ma and showed limited subsequent change. Hence, the overall structure of the deep Atlantic was largely unaffected by the early Pliocene CAS shoaling, corroborating other evidence that indicates larger changes in NADW resulted from earlier and deeper shoaling phases. This finding implies that the early Pliocene shoaling of the CAS had no profound impact on the evolution of climate

Earth and Planetary Science Letters 235 (2005) 45–61

Radiocarbon simulations for the glacial ocean: The effects of wind stress, Southern Ocean sea ice and Heinrich event

A new system for heterologous expression of membrane proteins: Rhodospirillum rubrum

a b s t r a c t Heterologous expression of membrane proteins has met with only limited success. This work presents a new host/vector system for the production of heterologous membrane proteins based on a mutant of the facultatively phototrophic bacterium Rhodospirillum rubrum. Under certain growth conditions, R. rubrum forms an intracytoplasmic membrane (ICM) that houses the photosynthetic apparatus, the structural proteins of which are encoded by puhA and pufBALM. The mutant R. rubrum H2, which was constructed by allelic exchange deleting puhA and pufBALM, does not form ICM. This strain was used as a host for a plasmid expressing the Pseudomonas aeruginosa membrane protein MscL from the Rhodobacter capsulatus puc promoter. ICM was formed in the H2 strain producing MscL but not in the vector control strain. These results suggest that a heterologous membrane protein stimulates ICM formation in R. rubrum and indicate that the capacity to form an ICM that can accommodate heterologous proteins makes R. rubrum a host that will be useful for membrane protein production. P. aeruginosa MscL, which forms inclusion bodies when produced in Escherichia coli, was expressed in R. rubrum H2 and purified from membranes with a yield of 22.8-23.4 mg/L culture (5.53-5.60 mg/g cell paste). Additionally Streptomyces lividans KcsA and P. aeruginosa CycB were produced and purified from R. rubrum H2 with yields of 13.7-14.4 mg/L culture (2.19-2.55 mg/g cell paste) and 6.6-7.4 mg/L culture (1.1-1.2 mg/g cell paste), respectively. Ó 2009 Elsevier Inc. All rights reserved. Introduction Overproduction of membrane proteins in heterologous systems has met with only limited success. While Escherichia coli is very useful for the production of many soluble proteins, expression of membrane proteins in this host may be lethal or result in the production of inclusion bodies formed by misfolded proteins. As a result of the difficulties in the production of membrane proteins in currently available systems, structural studies of membrane proteins have lagged significantly behind such studies of soluble proteins. A new bacterial system for the production of membrane proteins would provide the opportunity to expand the study of this important class of proteins. Rhodospirillum rubrum is a bacterium that is capable of phototrophic growth under anaerobic conditions in the light and chemotrophic growth under aerobic conditions in the dark. Under reduced oxygen conditions, R. rubrum forms an intracytoplasmic membrane (ICM) 2 that houses the photosynthetic apparatus. This apparatus consists of the light-harvesting antenna and the photochemical reaction center. Aerobically grown cells use aerobic respiratory metabolism and lack the photosynthetic apparatus and ICM. The proteins of the pigment-protein complexes in the ICM are encoded by puhA and pufBALM. Knockout mutants deleted in either puhA or pufBALM lack ICM, but ICM formation is restored by complementatio

Variable ventilation ages in the equatorial Indian Ocean thermocline during the LGM

Variations of atmospheric CO 2 during the Pleistocene ice-ages have been associated with changes in the drawdown of carbon into the deep-sea. Modelling studies suggest that about one third of the glacial carbon drawdown may not be associated to the deep ocean, but to the thermocline or intermediate ocean. However, the carbon storage capacity of thermocline waters is still poorly constrained. Here we present paired 230 Th/U and 14 C measurements on scleractinian cold-water corals retrieved from ~ 450 m water depth off the Maldives in the Indian Ocean. Based on these measurements we calculate ∆ 14 C, ∆∆ 14 C and Benthic-Atmosphere (B atm ) ages in order to understand the ventilation dynamics of the equatorial Indian Ocean thermocline during the Last Glacial Maximum (LGM). Our results demonstrate a radiocarbon depleted thermocline as low as -250 to -345‰ (∆∆ 14 C), corresponding to ~ 500–2100 years (B atm ) old waters at the LGM compared to ~ 380 years today. More broadly, we show that thermocline ventilation ages are one order of magnitude more variable than previously thought. Such a radiocarbon depleted thermocline can at least partly be explained by variable abyssal upwelling of deep-water masses with elevated respired carbon concentrations. Our results therefore have implications for radiocarbon-only based age models and imply that upper thermocline waters as shallow as 400 m depth can also contribute to some of the glacial carbon drawdown