Black spots and female association preferences in a sexual/asexual mating complex ( Poecilia , Poeciliidae, Teleostei)

We investigated whether female association preferences for males are influenced by black spot disease (BSD), a parasite induced change of the host phenotype. We compared three different species of fish: a gynogenetic hybrid species, Poecilia formosa (amazon molly) and two sexual species (Poecilia latipinna and Poecilia mexicana), which were involved in the natural hybridisation leading to the amazon molly. Contrary to their sexual relatives, asexual amazon mollies significantly avoided images of males infected with black spot disease. We propose that amazon molly females have direct fitness benefits from choosing healthy males. The adaptive significance of the preference for BSD-uninfected males in the asexual amazon molly is yet unclear but may involve avoidance of predation or parasite infection as well as increased sperm availabilit

ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable in vitro release results, the robustness of a commercially available ELISA for E. coli-derived BMP-2 and the parallel determination of BMP-2 recovery using a quantitative biological activity assay were investigated within a common release setup, with special reference to solubility and matrix effects. Without bovine serum albumin and Tween 20 as solubilizing additives to release media buffed at physiological pH, BMP-2 recoveries after release were notably reduced. In contrast, the addition of chitosan to release samples caused an excessive recovery. A possible explanation for these effects is the reversible aggregation tendency of BMP-2, which might be influenced by an interaction with chitosan. The interfering effects highlighted in this study are of great importance for bio-assay-based BMP-2 quantification, especially in the context of pharmaceutical release experiments

Neoadjuvant cisplatin and fluorouracil versus epirubicin, cisplatin, and capecitabine followed by resection in patients with oesophageal adenocarcinoma (UK MRC OE05): an open-label, randomised phase 3 trial.

BACKGROUND: Neoadjuvant chemotherapy before surgery improves survival compared with surgery alone for patients with oesophageal cancer. The OE05 trial assessed whether increasing the duration and intensity of neoadjuvant chemotherapy further improved survival compared with the current standard regimen. METHODS: OE05 was an open-label, phase 3, randomised clinical trial. Patients with surgically resectable oesophageal adenocarcinoma classified as stage cT1N1, cT2N1, cT3N0/N1, or cT4N0/N1 were recruited from 72 UK hospitals. Eligibility criteria included WHO performance status 0 or 1, adequate respiratory, cardiac, and liver function, white blood cell count at least 3 × 10(9) cells per L, platelet count at least 100 × 10(9) platelets per L, and a glomerular filtration rate at least 60 mL/min. Participants were randomly allocated (1:1) using a computerised minimisation program with a random element and stratified by centre and tumour stage, to receive two cycles of cisplatin and fluorouracil (CF; two 3-weekly cycles of cisplatin [80 mg/m(2) intravenously on day 1] and fluorouracil [1 g/m(2) per day intravenously on days 1-4]) or four cycles of epirubicin, cisplatin, and capecitabine (ECX; four 3-weekly cycles of epirubicin [50 mg/m(2)] and cisplatin [60 mg/m(2)] intravenously on day 1, and capecitabine [1250 mg/m(2)] daily throughout the four cycles) before surgery, stratified according to centre and clinical disease stage. Neither patients nor study staff were masked to treatment allocation. Two-phase oesophagectomy with two-field (abdomen and thorax) lymphadenectomy was done within 4-6 weeks of completion of chemotherapy. The primary outcome measure was overall survival, and primary and safety analyses were done in the intention-to-treat population. This trial is registered with the ISRCTN registry (number 01852072) and ClinicalTrials.gov (NCT00041262), and is completed. FINDINGS: Between Jan 13, 2005, and Oct 31, 2011, 897 patients were recruited and 451 were assigned to the CF group and 446 to the ECX group. By Nov 14, 2016, 327 (73%) of 451 patients in the CF group and 302 (68%) of 446 in the ECX group had died. Median survival was 23·4 months (95% CI 20·6-26·3) with CF and 26·1 months (22·5-29·7) with ECX (hazard ratio 0·90 (95% CI 0·77-1·05, p=0·19). No unexpected chemotherapy toxicity was seen, and neutropenia was the most commonly reported event (grade 3 or 4 neutropenia: 74 [17%] of 446 patients in the CF group vs 101 [23%] of 441 people in the ECX group). The proportions of patients with postoperative complications (224 [56%] of 398 people for whom data were available in the CF group and 233 [62%] of 374 in the ECX group; p=0·089) were similar between the two groups. One patient in the ECX group died of suspected treatment-related neutropenic sepsis. INTERPRETATION: Four cycles of neoadjuvant ECX compared with two cycles of CF did not increase survival, and cannot be considered standard of care. Our study involved a large number of centres and detailed protocol with comprehensive prospective assessment of health-related quality of life in a patient population confined to people with adenocarcinomas of the oesophagus and gastro-oesophageal junction (Siewert types 1 and 2). Alternative chemotherapy regimens and neoadjuvant chemoradiation are being investigated to improve outcomes for patients with oesophageal carcinoma. FUNDING: Cancer Research UK and Medical Research Council Clinical Trials Unit at University College London

KUGEL: Kommunikation mit unterstützenden Gebärden - ein Eltern-Kind-Gruppenprogramm zur systematischen Anleitung der engsten Bezugspersonen

Kinder mit einer globalen Entwicklungsstörung, z.B. im Rahmen eines genetischen Syndroms, weisen oft einen erheblich verspäteten Sprachbeginn auf. Um in dieser verlängerten präverbalen Phase Kindern und Eltern die Kommunikation miteinander zu erleichtern, hat sich der Einsatz lautsprachunterstutzender Gebärden LUG bewährt. Das Lernen der Gebärden fällt vielen Kindern leichter als das Sprechenlernen und stellt zudem eine „Brücke“ für den Spracherwerb dar. Voraussetzung dafür ist jedoch die kontinuierliche Anwendung der Gebärden im Alltag durch die Eltern. Viele Eltern haben allerdings Vorbehalte gegenüber LUG und brauchen viel Unterstützung beim Erlernen dieser. Aufgrund bisher fehlender Konzepte zur systematischen LUG-Anleitung der Eltern wurde ein Elterngruppenprogramm für Eltern (noch) nicht sprechender zwei- bis dreijähriger Kinder auf Basis des „Heidelberger Elterntrainings“ entwickelt: KUGEL: „Kommunikation mit unterstützenden Gebärden - ein Elterngruppenprogramm“. Bestandteile sind: 5 Elterngruppensitzungen, 1 Eltern-Kinder-Gruppentreffen, 1 Individualschulung sowie 3 Eltern Kinder-Gruppentreffen als Nachsorgeprogramm. KUGEL ist für eine Kleingruppe von max. 10 Personen konzipiert. In Kooperation des Werner-Otto-Instituts Hamburg mit dem ZEL Heidelberg wird ab 2014 die Effektivität von KUGEL im Hinblick auf die Verwendung von LUG bei Kind und Eltern in einer randomisiert kontrollierten Evaluationsstudie überprüft. Methodik: 50 Familien mit einem global entwicklungsgestörten und in der lautsprachlichen Entwicklung erheblich beeinträchtigen Kind werden nach erfolgter Entwicklungs-, Sprach- und Interaktionsdiagnostik randomisiert der Interventions- oder Kontrollgruppe zugewiesen. Die Eltern der Interventionsgruppe nehmen an KUGEL teil, die Eltern der KG erhalten eine eintägige Einweisung in die Anwendung von LUG. Die Nachuntersuchung findet direkt nach KUGEL statt. Es folgt ein Follow-up nach sechs Monaten. Auf der Tagung werden das Konzept von KUGEL sowie erste Ergebnisse zur Durchführung dieser elternzentrierten Intervention vorgestellt

Black spots and female association preferences in a sexual/asexual mating complex (Poecilia, Poeciliidae, Teleostei)

We investigated whether female association preferences for males are influenced by black spot disease (BSD), a parasite induced change of the host phenotype. We compared three different species of fish: a gynogenetic hybrid species, Poecilia formosa (amazon molly) and two sexual species (Poecilia latipinna and Poecilia mexicana), which were involved in the natural hybridisation leading to the amazon molly. Contrary to their sexual relatives, asexual amazon mollies significantly avoided images of males infected with black spot disease. We propose that amazon molly females have direct fitness benefits from choosing healthy males. The adaptive significance of the preference for BSD-uninfected males in the asexual amazon molly is yet unclear but may involve avoidance of predation or parasite infection as well as increased sperm availabilit

ELISA- and Activity Assay-Based Quantification of BMP-2 Released In Vitro Can Be Biased by Solubility in “Physiological” Buffers and an Interfering Effect of Chitosan

Chitosan nanogel-coated polycaprolactone (PCL) fiber mat-based implant prototypes with tailored release of bone morphogenic protein 2 (BMP-2) are a promising approach to achieve implant-mediated bone regeneration. In order to ensure reliable in vitro release results, the robustness of a commercially available ELISA for E. coli-derived BMP-2 and the parallel determination of BMP-2 recovery using a quantitative biological activity assay were investigated within a common release setup, with special reference to solubility and matrix effects. Without bovine serum albumin and Tween 20 as solubilizing additives to release media buffed at physiological pH, BMP-2 recoveries after release were notably reduced. In contrast, the addition of chitosan to release samples caused an excessive recovery. A possible explanation for these effects is the reversible aggregation tendency of BMP-2, which might be influenced by an interaction with chitosan. The interfering effects highlighted in this study are of great importance for bio-assay-based BMP-2 quantification, especially in the context of pharmaceutical release experiments

Reaching the Heart or the Mind? Test of two Theory-Based Training Programs to Improve Interactions Between Age-Diverse Coworkers

Due to demographic change, age diversity is increasing in many organizations. We aimed to understand how organizations can use age diversity training to overcome the challenges and realize the benefits of an age-diverse workforce. We built on the two predominant theoretical perspectives in the diversity literature–social identity theory and the information/decision-making perspective–to advance a dual pathway model and to develop two age diversity training programs: An identity-oriented training that helps organizations to overcome the challenges of age diversity by “speaking to the heart” of age-diverse coworkers and a knowledge-oriented training that helps organizations to realize the benefits of age diversity by “speaking to the mind” of age-diverse coworkers. We tested both training programs in a randomized controlled field experiment with age-diverse coworker dyads. We found that the identity-oriented training facilitated contact quality as a socioemotional outcome through increased levels of coworker’s perceived similarity and also reduced stereotype threat. The knowledge-oriented training increased knowledge transfer as a sociocognitive outcome through increased levels of coworker’s perceived knowledge utility and transactive memory. In a pilot training integration study, we made a first attempt to develop and test an integrated training program. Our findings advance research on the evidence-based management of age diversity

Alterations in cerebellar physiology are associated with a stiff-legged gait in Atcay(ji-hes) mice.

Recent evidence suggests that dystonia, a movement disorder characterized by sustained involuntary muscle contractions, can be associated with cerebellar abnormalities. The basis for how functional changes in the cerebellum can cause dystonia is poorly understood. Here we identify alterations in physiology in Atcay(ji-hes) mice which in addition to ataxia, have an abnormal gait with hind limb extension and toe walking, reminiscent of human dystonic gait. No morphological abnormalities in the brain accompany the dystonia, but partial cerebellectomy causes resolution of the stiff-legged gait, suggesting that cerebellar dysfunction contributes to the dystonic gait of Atcay(ji-hes) mice. Recordings from Purkinje and deep cerebellar nuclear (DCN) neurons in acute brain slices were used to determine the physiological correlates of dystonia in the Atcay(ji-hes) mice. Approximately 50% of cerebellar Purkinje neurons fail to display the normal repetitive firing characteristic of these cells. In addition, DCN neurons exhibit increased intrinsic firing frequencies with a subset of neurons displaying bursts of action potentials. This increased intrinsic excitability of DCN neurons is accompanied by a reduction in after-hyperpolarization currents mediated by small-conductance calcium-activated potassium (SK) channels. An activator of SK channels reduces DCN neuron firing frequency in acute cerebellar slices and improves the dystonic gait of Atcay(ji-hes) mice. These results suggest that a combination of reduced Purkinje neuron activity and increased DCN intrinsic excitability can result in a combination of ataxia and a dystonia-like gait in mice