COMPARATIVE EVALUATION OF SKIN SUTURE IN RATS WITH POLYGLYCAPRONE 25 AND NYLON

ABSTRACT Currently, the market offers a wide variety of suture threads, made of materials with different structural and chemical properties. Among many other characteristics, they vary in origin, absorption or degradation, and structure. From this variety, the clinical doubt arises as to which material provides the patient with the best healing quality. Objective: This study aims to comparatively evaluate two different types of suture threads-Monocryl® (polyglycaprone 25) and Ethilon® (nylon)-regarding their ability to aid in tissue regeneration by a histological and immunohistochemical analysis of the skin of rats sutured with the aforementioned materials. Methods: This basic experimental study used 12 adult Wistar rats, randomly divided into three groups with four animals each and subjected to four longitudinal incisions under anesthesia. Each group corresponded to a postsurgical evaluation date (one, seven, and 14 days). Results: At 14 postoperative days, the studied groups had no histological difference. However, the use of nylon thread showed greater evidence of earlier fibrotic union. Conclusion: This study found no histological difference in healing 14 days after surgery among the techniques and the types of suture threads. Level of Evidence II, Therapeutic Studies

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Burnout: o impacto da satisfação no trabalho em profissionais de enfermagem

O objetivo do artigo é analisar a relação entre fatores de satisfação no trabalho (satisfação com as relações hierárquicas; satisfação com o ambiente físico do trabalho; satisfação com o trabalho e oportunidade de crescimento) e dimensões da Síndrome de Burnout (exaustão emocional, despersonalização e realização profissional) em profissionais de enfermagem de um hospital público. Utilizou-se a abordagem quantitativa. A amostra foi composta por 52 enfermeiros. Os instrumentos aplicados foram: questionário sociodemografico, Maslach Burnout Inventory, Questionário de Satisfação no Trabalho S20/23. Para a análise utilizou-se a regressão linear múltipla dos Mínimos Quadrados Ordinários. Os resultados apresentaram que o fator satisfação com o ambiente reduz a exaustão emocional; o aumento com a satisfação com o trabalho e oportunidade de crescimento reduz as dimensões exaustão emocional e despersonalização; e também aumenta a realização profissional

Burnout: O impacto da satisfação no trabalho em profissionais de enfermagem

O objetivo do artigo é analisar a relação entre fatores de satisfação no trabalho (satisfação com as relações hierárquicas; satisfação com o ambiente físico do trabalho; satisfação com o trabalho e oportunidade de crescimento) e dimensões da Síndrome de Burnout (exaustão emocional, despersonalização e realização profissional) em profissionais de enfermagem de um hospital público. Utilizou-se a abordagem quantitativa. A amostra foi composta por 52 enfermeiros. Os instrumentos aplicados foram: questionário sociodemografico, Maslach Burnout Inventory, Questionário de Satisfação no Trabalho S20/23. Para a análise utilizou-se a regressão linear múltipla dos Mínimos Quadrados Ordinários. Os resultados apresentaram que o fator satisfação com o ambiente reduz a Exaustão Emocional; e o aumento com a satisfação com o trabalho e oportunidade de crescimento reduz as dimensões Exaustão Emocional e Despersonalização; e também aumenta a Realização Profissional

Factors underlying risk taking in heroin-dependent individuals : feedback processing and environmental contingencies

Evidence suggests that factors influencing risk-taking include whether decisions are made based on emotions (affective systems) or cognitions (deliberative systems), the processing of feedback (e.g., deciding to attend are habilitation facility for opioid addiction treatment after an intervention held by a family member), and attention to environmental contingencies (e.g., considering the probability of an outcome such as the likelihood of contracting tetanus from a shared needle; or the gains and losses associated with a decision, such as the benefits and costs of taking drugs). Although drug-dependent individuals tend to take more risks than non-drug users, the factors underlying risk-taking are unknown. The current study tested, for the first time, the influences of performance feedback (i.e., whether feedback about performance is integrated into decision-making in heroin-dependent individuals) and attention to environmental contingencies (i.e., the influence of the probability of a loss, the gain amount, and the loss amount associated with a scenario) on risk-taking in heroin-dependent individuals. Heroin-dependent patients undergoing maintenance therapy for opioid addiction (n = 25) and healthy controls (n = 27) completed the feedback and no-feedback conditions of the Columbia Card Task (CCT).Analyses of covariance, controlling for education and task design (the order in which the CCT conditions were completed) as covariates revealed a significant interaction between (a) probability, gain and loss amount, and group, and (b) group and probability. Our findings suggest that heroin-dependent patients pay less attention to environmental contingencies during risk-taking than controls. Addressing these factors may facilitate greater adherence to treatment programs and lower rates of relapse

METACARPAL FRACTURES TREATMENT: COMPARASION BETWEEN KIRSCHNER WIRE AND INTRAMEDULLARY SCREW

ABSTRACT Introduction: Metacarpal fractures are common and can be treated surgically using Kirschner wires (K-wires) or intramedullary fixation with compression screws (IMCS). Objectives: Analyze the postsurgical results from treating the metacarpal extra-articular fractures through the retrograde Kirschner wire technique, and compare it with the intramedullary compression screw fixation. Methods: Retrospective and quantitative studies were to analyze patients’ medical records, and a postsurgical evaluation questionnaire was given to the patients, who were divided into K-wire and IMCS. Results: The period of immobilization with a splint took six weeks for the K-wire group and four weeks for the IMCS group. The average time for consolidation took, respectively, fifty-seven days and forty-seven days. The first group could restart their activities twenty-two days after the other, and the average force value of the treated hand, when compared with its contralateral, was 93.9% and 95.4%, respectively. Between the operated hand and its contralateral, there was a difference of 16° in the total measures of the metacarpophalangeal and interphalangeal joint's range of movement among the K-wire group and 5° among the IMCS group. Conclusion: The patients who participated in this study showed excellent results after surgery, and both treatments were proven to be safe and reliable. Evidence level III; Retrospective comparative study

Efficacy of Commercial Phage Based Treatment as a Control Strategy against Listeria Spp. and Effect of Host Characteristics on Lytic Capacity

Listeria monocytogenes can persist on food contact (FCS) and non-food contact surfaces (NFCS) and enter the food continuum by cross-contamination. Meat processing facilities are a known source of Listeria spp. contamination. Specificity and safety of use render bacteriophages suitable to control Listeria spp. in foods and food environments. Previous research has shown that host characteristics can affect lytic efficacy of bacteriophages. This study focuses on evaluating how phenotypic and genotypic characteristics of Listeria hosts influence lytic capacity of a commercial listeriophage cocktail in vitro. We also investigated the efficacy of listeriophage as a biocontrol strategy for Listeria spp. on non-food contact surfaces (NFCS) in a meat processing facility. In vitro lytic capacity was tested quantitatively using spot assay for 475 Listeria spp. isolates with varied phenotypic (attachment capacity, sanitizer tolerance) and genotypic (PFGE) characteristics. Lytic capacity was measured quantitatively for 55 isolates by monitoring growth of L. monocytogenes cultures with and without listeriophage over time and enumerating bacterial counts after 4h. Fifty-nine NFCS were tested in a meat processing facility for Listeria spp. weekly for three weeks. Each Listeria spp. positive site was treated with commercial phage then assessed for reduction in Listeria spp. Although enhanced attachment capacity and sanitizer tolerance of Listeria spp. isolates did not significantly (p\u3e0.05) influence phage susceptibility in vitro; history of persistence, incubation temperature, and concentration of listeriophage treatment were critical. Quantitatively, listeriophage treatment significantly (p\u3c0.001) affected growth and reduced bacterial counts of Listeria spp. compared to control samples. A total of 15, 21, and 14 sites were positive for Listeria spp. at weeks one, two, and three, respectively. Post-treatment Listeria spp. were detected in 12/23 sites, and numerically reduced in 4/12 sites by an average of 2.1 log CFU/sponge. Among the isolates subjected to spot assay, 60% strains showed low susceptibility to listeriophage, 36% showed moderate lysis, and 4% isolates showed confluent lysis by listeriophage. This study illustrates influence of bacterial host characteristics on lytic efficacy of listeriophage treatment. Further, we have preliminary evidence for listeriophage as a potential control strategy in food environments

DataSheet5_Loss of the fructose transporter SLC2A5 inhibits cancer cell migration.PDF

Metastasis is the primary cause of cancer patient death and the elevation of SLC2A5 gene expression is often observed in metastatic cancer cells. Here we evaluated the importance of SLC2A5 in cancer cell motility by silencing its gene. We discovered that CRISPR/Cas9-mediated inactivation of the SLC2A5 gene inhibited cancer cell proliferation and migration in vitro as well as metastases in vivo in several animal models. Moreover, SLC2A5-attenuated cancer cells exhibited dramatic alterations in mitochondrial architecture and localization, uncovering the importance of SLC2A5 in directing mitochondrial function for cancer cell motility and migration. The direct association of increased abundance of SLC2A5 in cancer cells with metastatic risk in several types of cancers identifies SLC2A5 as an important therapeutic target to reduce or prevent cancer metastasis.</p