Tursiops no Atlântico Sul Ocidental: comparação filogenética in silico com as espécies das bacias oceânicas adjacentes

Introdução O gênero Tursiops apresenta uma classificação taxonômica complexa, porém não há dúvidas de que T. gephyreus é uma linhagem diferente de T. truncatus. Além disso, não há estudos publicados de análise molecular incluindo T. aduncus, T. gephyreus e T. truncatus, sendo assim são necessários mais estudos para esclarecer a relação filogenética entre essas espécies. Objetivos Este estudo objetivou realizar uma busca em "silico" das sequências nucleotídicas depositadas do Gênero Tursiops, Gervais 1855 com ênfase em T. gephyreus, Lahille, 1908. O objetivo foi verificar que marcadores estavam disponíveis e se seria possível realizar análises filogenéticas entre espécies do gênero Tursiops do Atlântico Sul Ocidental e bacias oceânicas adjacentes, com o intuito de entender como T. gephyreus se relaciona filogeneticamente com outras linhagens do gênero. Métodos Realizou-se um levantamento das sequências de Tursiops disponibilizadas no GenBank do NCBI. Essas sequências foram alinhadas e utilizadas na construção de uma árvore filogenética. Utilizando sequências selecionadas a partir dessas 1794 sequências iniciais e sequências obtidas posteriormente realizou-se outras duas árvores filogenéticas (utilizando Sotalia fluviatilis como grupo externo e outras 6 espécies de Delfinídeos), gráficos de análise PcoA e uma tabela de distância genética. Resultados Foram encontradas 1794 sequências, de região controle mitocondrial e D-loop, do Gênero Tursiops, sendo 1.106 de T. truncatus, 198 de T. aduncus, 51 de T. gephyreus, 6 de T. australis. A partir da construção das árvores filogenéticas, dos gráficos e da tabela de distâncias genéticas observou-se que em todos os métodos T. gephyreus e T. truncatus agruparam-se. Discussão A análise dos resultados demonstrou a baixa quantidade de amostras sequenciadas para T. gephyreus e da variedade de marcadores moleculares utilizados para a obtenção das mesmas. Mesmo assim, ao refinar as buscas utilizando sequências de trabalhos selecionados, foi possível hipotetizar algumas relações entre as espécies. O baixo número amostral de sequências e genes de T. gephyreus não permitiu realizar análises mais robustas.Introduction The genus Tursiops has a complex taxonomic classification, but there is no doubt that T. gephyreus is a different lineage from T. truncatus. In addition, there are no published molecular analyses studies T. aduncus, T. gephyreus and T. truncatus, so further studies are needed to clarify the phylogenetic relationship between these species. Goals This study was aimed at performing an “in silico'' search of the nucleotide sequences deposited from the genus Tursiops, Gervais, 1855 with emphasis on T. gephyreus, Lahille, 1908. The objective was to verify which markers were available and whether it would be possible to perform phylogenetic analysis between species of the genus Tursiops from the Western South Atlantic and adjacent ocean basins, in order to understand how T. gephyreus is phylogenetically related to other strains of the genus. Methods A survey of the Tursiops sequences available from NCBI's GenBank was carried out. These sequences were aligned and used in the construction of a phylogenetic tree. Using sequences selected from these 1794 initial sequences and sequences obtained later two other phylogenetic trees were created (using Sotalia fluviatilis as an outgroup and another 6 species of Delphinidae), PcoA analysis graphs and a genetic distance table. Results A total of 1794 sequences from the mitochondrial control region and D-loop of the genus Tursiops were found, 1106 from T. truncatus, 198 from T. aduncus, 51 from T. gephyreus, 6 from T. australis. From the construction of phylogenetic trees, graphs and the table of genetic distances, it was observed that in all methods T. gephyreus and T. truncatus were grouped. Discussion The analysis of the results demonstrated the low amount of samples sequenced for T. gephyreus and the variety of molecular markers used to obtain them. Even so, by refining the searches, using sequences of selected works, it was possible to hypothesize some relationships between the species. The low sample number of T. gephyreus sequences and genes did not allow for more robust analyses

Diálogos entre o MUCIN e Comunidades Tradicionais do Litoral Norte do RS 2019

O MUCIN tem adotado como política de atuação conhecer e envolver as comunidades do seu entorno, com as quais o diálogo é importante na busca por contribuir com o desenvolvimento sustentável

Diálogos entre o MUCIN e Comunidades Tradicionais do Litoral Norte do RS 2019

O MUCIN tem adotado como política de atuação conhecer e envolver as comunidades do seu entorno, com as quais o diálogo é importante na busca por contribuir com o desenvolvimento sustentável

Quality Of Life, Depression And Anxiety Among Pregnant Women With Previous Adverse Pregnancy Outcomes.

Previous adverse pregnancy outcomes (recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death) can affect the quality of life of pregnant women. The objective of this study was to compare the quality of life and the prevalence of symptoms of anxiety and depression among pregnant women with and without these antecedents. An analytical cross-sectional study was performed in four settings (two high-risk and two low-risk prenatal clinics) in the city of Campinas, São Paulo, Brazil. A total of 240 women were interviewed by a single investigator between the 18th and 24th weeks of gestation: 120 women with prior adverse pregnancy outcomes (group 1) and 120 women with no such history (group 2), matched according to their numbers of living children. Sociodemographic variables were collected and two questionnaires were used: the Short Form-36 quality-of-life questionnaire and the Depression and Anxiety Scale. The women in group 1 had lower scores in all the items on the quality-of-life questionnaire. Depression and anxiety were more frequent in group 1 (P < 0.0001). An inverse correlation was found between the Short Form-36 domains and anxiety and depression. Women with histories of recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death seem to have poorer quality of life and more symptoms of anxiety and depression during their subsequent pregnancy, compared with those without such antecedents.127185-

Quality of life, depression and anxiety among pregnant women with previous adverse pregnancy outcomes

CONTEXT AND OBJECTIVE: Previous adverse pregnancy outcomes (recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death) can affect the quality of life of pregnant women. The objective of this study was to compare the quality of life and the prevalence of symptoms of anxiety and depression among pregnant women with and without these antecedents. DESIGN AND SETTING: An analytical cross-sectional study was performed in four settings (two high-risk and two low-risk prenatal clinics) in the city of Campinas, São Paulo, Brazil. METHODS: A total of 240 women were interviewed by a single investigator between the 18th and 24th weeks of gestation: 120 women with prior adverse pregnancy outcomes (group 1) and 120 women with no such history (group 2), matched according to their numbers of living children. Sociodemographic variables were collected and two questionnaires were used: the Short Form-36 quality-of-life questionnaire and the Depression and Anxiety Scale. RESULTS: The women in group 1 had lower scores in all the items on the quality-of-life questionnaire. Depression and anxiety were more frequent in group 1 (P < 0.0001). An inverse correlation was found between the Short Form-36 domains and anxiety and depression. CONCLUSIONS: Women with histories of recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death seem to have poorer quality of life and more symptoms of anxiety and depression during their subsequent pregnancy, compared with those without such antecedents

Qualidade de vida, depressão e ansiedade em gestantes com má história gestacional

CONTEXT AND OBJECTIVE: Previous adverse pregnancy outcomes (recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death) can affect the quality of life of pregnant women. The objective of this study was to compare the quality of life and the prevalence of symptoms of anxiety and depression among pregnant women with and without these antecedents. DESIGN AND SETTING: An analytical cross-sectional study was performed in four settings (two high-risk and two low-risk prenatal clinics) in the city of Campinas, São Paulo, Brazil. METHODS: A total of 240 women were interviewed by a single investigator between the 18th and 24th weeks of gestation: 120 women with prior adverse pregnancy outcomes (group 1) and 120 women with no such history (group 2), matched according to their numbers of living children. Sociodemographic variables were collected and two questionnaires were used: the Short Form-36 quality-of-life questionnaire and the Depression and Anxiety Scale. RESULTS: The women in group 1 had lower scores in all the items on the quality-of-life questionnaire. Depression and anxiety were more frequent in group 1 (P < 0.0001). An inverse correlation was found between the Short Form-36 domains and anxiety and depression. CONCLUSIONS: Women with histories of recurrent spontaneous abortion, fetal death, preterm birth or early neonatal death seem to have poorer quality of life and more symptoms of anxiety and depression during their subsequent pregnancy, compared with those without such antecedents.CONTEXTO E OBJETIVO: O antecedente de resultados gestacionais adversos (aborto espontâneo recorrente, óbito fetal, prematuridade ou óbito neonatal precoce) pode afetar a qualidade de vida das gestantes. O objetivo deste estudo foi comparar a qualidade de vida e a prevalência de sintomas de ansiedade e depressão em gestantes com e sem estes antecedentes. TIPO DE ESTUDO E LOCAL: Um estudo transversal analítico foi realizado em quatro locais (duas clínicas de pré-natal de alto risco e duas de pré-natal de baixo risco), na cidade de Campinas, São Paulo, Brasil. MÉTODOS: Duzentas e quarenta mulheres foram entrevistadas por um único investigador entre 18 e 24 semanas de gestação: 120 tinham antecedentes gestacionais adversos (grupo 1) e 120 não tinham tal história (grupo 2), pareadas por número de filhos vivos. Variáveis sócio-demográficas foram coletadas e dois questionários foram usados: o de qualidade de vida (Questionário Short Form-36) e a Escala de Depressão e Ansiedade. RESULTADOS: As mulheres do grupo 1 obtiveram escores mais baixos em todos os itens do questionário de qualidade de vida. Depressão e ansiedade foram mais frequentes no grupo 1 (P < 0,0001). Foi encontrada uma correlação inversa entre os domínios do Short Form-36 e ansiedade e depressão. CONCLUSÕES: Mulheres com antecedente de aborto espontâneo recorrente, óbito fetal, prematuridade ou óbito neonatal precoce parecem ter pior qualidade de vida e mais sintomas de ansiedade e depressão durante a gestação subsequente quando comparadas com mulheres sem esses antecedentes.18518