PP15 - Toxina botulínica no tratamento do bruxismo em pacientes com encefalopatia crônica não progressiva-revisão de literatura

INTRODUÇÃO: Encefalopatia Crônica Não Progressiva (ECP) é caracterizada por lesão cerebral não progressiva, constituindo a causa mais frequente de deficiência física infantil. Os problemas neuromusculares afetam a saúde bucal, sendo o bruxismo um achado frequente, que é caracterizado pela aplicação de forças excessivas nos músculos mastigatórios, podendo causar disfunção da articulação têmporo-mandibular, dores de cabeça e facetas de desgaste dentário. Diversos tratamentos sem sucesso para o bruxismo na ECP são descritos; entretanto, recentemente a injeção local da toxina botulínica foi relatada como excelente opção

PP14 - Contribuição da sedação com benzodiazepínico no atendimento a pacientes com necessidades especiais não colaboradores

INTRODUÇÃO: A sedação com benzodiazepínico é um recurso que pode ser utilizado para possibilitar o tratamento odontológico em pacientes com necessidades especiais (PNE). Esta é indicada principalmente para controle da ansiedade, medo, reflexo de vômito, secreção de saliva e movimentos incoordenados

Prevalence and heritability of psoriasis and benign migratory glossitis in one Brazilian population

Background: An oral condition associated to psoriasis is benign migratory glossitis. The review of the literature does not show any publication about heritability in both psoriasis and benign migratory glossitis and prevalence of psoriasis in the Brazilian population. Objective: This research was carried out in order to determine the prevalence of psoriasis and benign migratory glossitis in the Brazilian population from a Brazilian sample, as well as the heritability in these conditions. Methods: Six thousand patients were studied from the records of the outpatient dermatology department. The sample had 129 patients with cutaneous psoriasis, 399 with benign migratory glossitis without psoriasis and a control group with 5,472 patients. After data collection, the statistical analysis was made using Woolf, Chi-square and Falconer tests. Results: The prevalence of psoriasis was 2.15% and the benign migratory glossitis was 7.0%. The prevalence of benign migratory glossitis in the psoriasis group was high (16.3%), and that was statistically significant. Family history in the psoriasis group was 38% for the condition itself and 2,75% for benign migratory glossitis and in the benign migratory glossitis group was 17.54% for the condition itself and 1.5% for psoriasis. The study of heritability was 38.8% for psoriasis and 36.6% for benign migratory glossitis, both with medium heritability. Study limitations: This study was only in the state of Sao Paulo. Conclusion: This is the first publication that quantifies how much of these conditions have a genetic background and how important the environmental factors are in triggering them.Univ Marilia Unimar, Sch Med, Discipline Dermatol, Marilia, SP, BrazilCESD, Res Ctr, Dracena, SP, BrazilUniv Fed Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilUniv Fed Fluminense, Sch Med, Dept Pathol, Niteroi, RJ, BrazilUniv Fed Sao Carlos UFSCar, Fac Hlth Sci, Dept Genet & Evolut, Sao Carlos, SP, BrazilUniv Fed Sao Paulo Unifesp, Dept Dermatol, Sao Paulo, SP, BrazilWeb of Scienc

Comparative analysis of Gram’s method and PAS for the identification ofCandidaspp. samples from the oral mucosa

Introduction: Candida species are part of the normal microbiota of healthy subjects, living as commensals. However, they can become pathogenic when changes in the mechanisms of host defense or disruption of anatomic barriers occur. Candidiasis is the most common fungal infection in the oral cavity, mainly caused by Candida albicans. The diagnosis is based on symptoms and clinical aspects, in association with laboratory methods. Objective: To evaluate the sensitivity and specificity of Gram’s method for Candida spp. identification in scrapes from the buccal mucosa and evaluate the degree of concordance between clinical and cytological methods in the diagnosis of oral candidiasis. Material and methods: A blind study was performed in 170 smears from patients of Hospital Universitário Antônio Pedro of Universidade Federal Fluminense (HUAP/UFF), stained by Gram (n = 57), periodic acid-Schiff (PAS) (n = 57) and Papanicolaou (Pap) (n = 57) methods. Results: The comparative analysis of the methods demonstrated a higher prevalence of Candida spp. (12%) in PAS than in Gram staining, without statistic significance. The cytology method was positive in 93% of the clinical diagnosis of candidiasis. Conclusion: Gram was an adequate method; however more intensive professional training would be necessary to identify the fungus morphological structures. Although Pap test is the most common method of routine cytopathologic examination, for candidiasis diagnosis PAS staining is also recommended. Thus, it is suggested that candidiasis diagnosis should be accomplished by clinical evaluation in association with cytopathological analysis based on the identification of hyphae and/or pseudohyphae

INVESTIGAÇÃO DA ASSOCIAÇÃO ENTRE LÍNGUA GEOGRÁFICA E PSORÍASE ATRAVÉS DA ANÁLISE DAS FREQUENCIAS DOS GENES HLA E KIR.

INTRODUÇÃO: A psoríase é uma doença cutânea-articular, imunologicamente mediada de base genética. A presença de lesão oral é controversa, sendo a língua geográfica (LG) considerada a manifestação oral mais associada à psoríase. Alguns estudos demonstram uma relação direta entre a psoríase, a LG e as moléculas de Antígenos Leucocitários Humanos (HLA). Além disso, estudos genéticos recentes correlacionaram determinados genes para receptores semelhantes à imunoglobulina para células Natural Killer (KIR) com o aumento da susceptibilidade à psoríase vulgar não sendo realizado nenhum trabalho sobre a relação da LG e estes genes. Apesar daassociação genética entre estas condições, as informações são limitadas e imprecisas não permitindo o esclarecimento da etiopatogenia da LG e confirmação desta relação. OBJETIVO: Diante disto, o objetivo deste estudo foi demonstrar a existência de similaridade na expressão dos genes HLA e KIR entre indivíduos com LG e psoríase. MÉTODOS: A amostra foi constituída por 174 participantes, divididos em 58 com psoríase (GT-P), 30 com LG sem psoríase (GT-LG) e 86 indivíduos saudáveis (grupo controle - GC), submetidos à coleta de sangue periférico para extração de DNA e análises moleculares. RESULTADOS: O alelo HLA-B*57 apresentou-se significativo na Psoríase e o HLA-B*58 na LG, sendo ambos representados pelo mesmo antígeno -B17 identificado por métodos sorológicos. Os genes KIR2DS1, KIR2DL5, KIR3DS1 apresentaram maior frequência na LG e psoríase, assim como o haplótipo B de genes KIR. Verificou-se frequências semelhantes entre KIR2DS2 e ligantes HLA-C1 e KIR3DS1 e ligantes HLA-Bw4 nos grupos testes. CONCLUSÃO: Há similaridade na expressão dos genes HLA e KIR entre indivíduos com LG e com Psoríase, reforçando a associação entre essas condições

TRATAMENTO RESTAURADOR CONSERVADOR NA AMELOGÊNESE IMPERFEITA - RELATO DE CASO CLÍNICO.

A amelogênese imperfeita (AI) compreende um amplo grupo de anomalias genéticas que afetam a formação do esmalte pela diferenciação imprópria dos ameloblastos, podendo apresentar-se clinicamente como hipoplásica, hipomaturada e hipocalcificada. O objetivo deste trabalho é relatar um caso clínico onde se optou por realizar um plano de tratamento restaurador conservador em paciente portador de AI. Paciente do sexo masculino, 15 anos, melanoderma com queixa de sensibilidade dentária. Na anamnese não foi registrada história pregressa de AI familiar, entretanto os genitores são primos em primeiro grau. Ao exame físico intraoral foi observado coloração amarelo acastanhado em todos os dentes permanentes seguida de manchas brancas ativas nas superfícies vestibular e oclusal/incisal dos elementos dentários. Vários elementos dentários apresentavam alterações forma e tamanho, devido ao acelerado desgaste das estruturas dentárias. Os elementos 36 e 46 apresentavam coroas metálicas bem adaptadas, palato ogival e, além disso, presença de biofilme visível, hiperemia gengival e sangramento em algumas regiões da arcada, língua saburrosa e halitose. Na radiografia panorâmica, foram observadas coroas mais quadrangulares nos incisivos e cúspides de molares e pré-molares reduzidas, além de imagem radiolúcida nas coroas de todos os dentes permanentes, compatível com AI do tipo hipocalcificada. Foi proposto plano de tratamento conservador a fim de estabelecer a função preservando ainda o crescimento maxilo-mandibular do paciente. O diagnóstico precoce da AI é de suma importância para um tratamento mais conservador e com enfoque na prevenção dos efeitos dessa anomalia

Intraoral soft tissue lipomas:clinicopathological features from 91 cases diagnosed in a single Oral Pathology service

intraoral soft tissue lipomas are relatively uncommon mesenchymal neoplasms. Few papers have been published comparing the clinicopathological features of these tumors in different populations. The aim of the present study was to analyze the clinicopathological features from intraoral soft tissue lipomas diagnosed in a Brazilian population. all cases diagnosed as intraoral soft tissue lipomas in an Oral Pathology laboratory from 2005 to 2019 were retrieved and descriptively analyzed; statistical analysis was performed for comparison of the clinical and demographic parameters. 91 intraoral lipomas were retrieved, including 56 lipomas, 30 fibrolipomas, 2 spindle cell lipomas, 2 angiolipomas, and 1 chondrolipoma. Mean age of the patients was 62.2 years and females represented 57.1% of the sample. Mean time of complaint was 45.4 months and mean size of the lesions was 16.2 millimeters. Buccal mucosa (38.8%), lower lip (18.8%) and tongue (16.5%) were the most commonly affected locations. Fibrolipomas were more common in females (p=0,037) and presented as smaller lesions (p=0,011) in comparison to lipomas. report of clinicopathological data from intraoral lipomas aid in establishing their differential diagnostic criteria and clinical profile in this specific location

Contribution of benzodiazepines in dental care of patients with special needs

Conscious sedation in dental treatment of patients with special needs (PNEs) has the purpose of controlling events such as anxiety and fear, as well as promoting muscle relaxation and mastery of uncoordinated movements. Benzodiazepines (BZDs) are among the most used drugs due to their anxiolytic, hypnotic and sedative properties. The objective of this investigation is to demonstrate a study on the contribution of conscious sedation with BZD in PNEs. The study included 40 PNEs, non-collaborators, submitted to conscious oral sedation with Midazolam (0.5 mg/kg) for dental treatment, receiving vital signs monitoring in the pre, trans and postoperative periods. Male patients were more frequent with 70% of the cases, with a mean age of 18 years. As for medical diagnosis, autism and mental deficiency were the most prevalent. The most performed procedures were restoration (32%) and exodontia (30%). There was a statistically significant reduction in systolic and diastolic blood pressure parameters (p<0.05) in the transoperative and postoperative periods when compared to the preoperative period. Conscious sedation with BZDs resulted in 83% positive responses. The results demonstrate that this technique is safe and effective, and can be used in outpatient care for PNEs. However, the risk/benefit ratio should be correctly evaluated