Investigação da associação do polimorfismo do gene NFKB1 no risco de doença arterial coronariana

A principal doença cardiovascular é a doença arterial coronariana (DAC), cuja patologia é de origem inflamatória crônica, multifatorial, desencadeada por fatores genéticos, ambientais e de grande incidência mundial, apresentando, múltiplos estágios no desenvolvimento da aterosclerose. Os fatores de risco que predispõem o surgimento e o desencadeamento de DAC, são classificados como modificáveis e não-modificáveis. Por ser uma doença multifatorial, além da presença de fatores de risco para a patologia, diversos estudos têm demonstrado que o desenvolvimento da DAC tem forte influência de fatores genéticos, tais como os polimorfismos no gene NFKB, regulador mestre de ativação da cascata inflamatória. Por conseguinte, este estudo tem como objetivo a verificação da relação do polimorfismo NFKB -94 ATTG ins/del com a presença de DAC em indivíduos adultos, submetidos ao serviço de Hemodinâmica do Hospital Bruno Born de Lajeado, Rio Grande do Sul, Brasil. A amostra totalizou 324 indivíduos adultos de ambos os sexos. As amostras de sangue periférico foram coletadas para as dosagens bioquímicas e moleculares. Foi utilizada a técnica da reação em cadeia da polimerase (PCR) para a amplificação do polimorfismo, com primers em condições específicas e, posteriormente, foi realizada digestão enzimática para genotipagem do polimorfismo deste estudo, onde os fragmentos resultantes foram visualizados em gel de agarose 2%. Os testes estatísticos foram realizados através do software SPSS, versão 21.0. Para as frequências genotípicas, foi utilizado o teste do qui-quadrado de Pearson e as variáveis categóricas pelo método de Mann Whitney Kruskal-Wallis. Para o equilíbrio de Hardy-Weinberg foi utilizado o teste do qui-quadrado. Não foram identificadas associações significativas com nenhuma das variáveis investigadas. Os achados sugerem que o polimorfismo investigado não apresenta um papel significativo na doença arterial coronariana.The main coronary artery diseases (CAD), whose pathology has a chronic inflammatory origin, multifactorial unleashed by genetic, environmental factors with a high incidence in the world. The risk factors that predispose the beginning and triggering of CAD are classified as modifiable and non-modifiable. As it is a multifactorial disease is known that besides the existence of risk factor for the pathology, numerous studies have been showing that the development of CAD has a strong influence on the genetic factors such as the polymorphism NFKB gene, main regulator of inflammatory cascade. This way, this study has as objective the verification of the relationship between -94 ATTG ins/del polymorphism of NFKB and the presence of CAD in adults, submitted through the Hemodinamic service of Bruno Born Hospital of Lajeado, Brazil. The sample of peripheral blood were collected for biochemistry and molecular analysis. The polymerase Chain Reaction (PCR) was used for the amplification of polymorphism, with primers in specific conditions, and later was done an enzymatic digestion for polymorphism genotyping -94 ATTG ins/del of gene NFKB1, where the resulting fragments were visualized on 2% agarose gel. The static test were done using the software SPSS version 21.0. for the genotype frequencies the chi-squared of Pearson test and the categorical variable by the method of Mann Whitney Kuskal Wallis were used. There was not found any significant association with any of the variables investigated. The findings suggest that the investigated polymorphism doesn’t presnt any significant role in the Coronary Artery Disease

Star formation and UV colors of the brightest Cluster Galaxies in the representative XMM-Newton Cluster Structure Survey

We present UV broadband photometry and optical emission-line measurements for a sample of 32 Brightest Cluster Galaxies (BCGs) in clusters of the Representative XMM-Newton Cluster Structure Survey (REXCESS) with z = 0.06-0.18. The REXCESS clusters, chosen to study scaling relations in clusters of galaxies, have X-ray measurements of high quality. The trends of star formation and BCG colors with BCG and host properties can be investigated with this sample. The UV photometry comes from the XMM Optical Monitor, supplemented by existing archival GALEX photometry. We detected H\alpha and forbidden line emission in 7 (22%) of these BCGs, in optical spectra. All of the emission-line BCGs occupy clusters classified as cool cores, for an emission-line incidence rate of 70% for BCGs in cool core clusters. Significant correlations between the H\alpha equivalent widths, excess UV production in the BCG, and the presence of dense, X-ray bright intracluster gas with a short cooling time are seen, including the fact that all of the H\alpha emitters inhabit systems with short central cooling times and high central ICM densities. Estimates of the star formation rates based on H\alpha and UV excesses are consistent with each other in these 7 systems, ranging from 0.1-8 solar masses per year. The incidence of emission-line BCGs in the REXCESS sample is intermediate, somewhat lower than in other X-ray selected samples (-35%), and somewhat higher than but statistically consistent with optically selected, slightly lower redshift BCG samples (-10-15%). The UV-optical colors (UVW1-R-4.7\pm0.3) of REXCESS BCGs without strong optical emission lines are consistent with those predicted from templates and observations of ellipticals dominated by old stellar populations. We see no trend in UV-optical colors with optical luminosity, R-K color, X-ray temperature, redshift, or offset between X-ray centroid and X-ray peak ().Comment: 19 pages, 18 figures, 6 tables. Submitted, with minor revisions, to ApJ

Study design and rationale for a randomized controlled trial to assess effectiveness of stochastic vibrotactile mattress stimulation versus standard non-oscillating crib mattress for treating hospitalized opioid-exposed newborns

The incidence of Neonatal Abstinence Syndrome (NAS) continues to rise and there remains a critical need to develop non-pharmacological interventions for managing opioid withdrawal in newborns. Objective physiologic markers of opioid withdrawal in the newborn remain elusive. Optimal treatment strategies for improving short-term clinical outcomes and promoting healthy neurobehavioral development have yet to be defined. This dual-site randomized controlled trial (NCT02801331) is designed to evaluate the therapeutic efficacy of stochastic vibrotactile stimulation (SVS) for reducing withdrawal symptoms, pharmacological treatment, and length of hospitalization, and for improving developmental outcomes in opioid-exposed neonates. Hospitalized newborns (n = 230) receiving standard clinical care for prenatal opioid exposure will be randomly assigned within 48-hours of birth to a crib with either: 1) Intervention (SVS) mattress: specially-constructed SVS crib mattress that delivers gentle vibrations (30-60 Hz, ~12 mum RMS surface displacement) at 3-hr intervals; or 2) Control mattress (treatment as usual; TAU): non-oscillating hospital-crib mattress. Infants will be studied throughout their hospitalization and post discharge to 14-months of age. The study will compare clinical measures (i.e., withdrawal scores, cumulative dose and duration of medications, velocity of weight gain) and characteristic progression of physiologic activity (i.e., limb movement, cardio-respiratory, temperature, blood-oxygenation) throughout hospitalization between opioid-exposed infants who receive SVS and those who receive TAU. Developmental outcomes (i.e., physical, social, emotional and cognitive) within the first year of life will be evaluated between the two study groups. Findings from this randomized controlled trial will determine whether SVS reduces in-hospital severity of NAS, improves physiologic function, and promotes healthy development

Study of the B−→Λc+Λˉc−K−B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-} decay

The decay $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ is studied in proton-proton collisions at a center-of-mass energy of $\sqrt{s}=13$ TeV using data corresponding to an integrated luminosity of 5 $\mathrm{fb}^{-1}$ collected by the LHCb experiment. In the $\Lambda_{c}^+ K^{-}$ system, the $\Xi_{c}(2930)^{0}$ state observed at the BaBar and Belle experiments is resolved into two narrower states, $\Xi_{c}(2923)^{0}$ and $\Xi_{c}(2939)^{0}$, whose masses and widths are measured to be $$m(\Xi_{c}(2923)^{0}) = 2924.5 \pm 0.4 \pm 1.1 \,\mathrm{MeV}, \\ m(\Xi_{c}(2939)^{0}) = 2938.5 \pm 0.9 \pm 2.3 \,\mathrm{MeV}, \\ \Gamma(\Xi_{c}(2923)^{0}) = \phantom{000}4.8 \pm 0.9 \pm 1.5 \,\mathrm{MeV},\\ \Gamma(\Xi_{c}(2939)^{0}) = \phantom{00}11.0 \pm 1.9 \pm 7.5 \,\mathrm{MeV},$$ where the first uncertainties are statistical and the second systematic. The results are consistent with a previous LHCb measurement using a prompt $\Lambda_{c}^{+} K^{-}$ sample. Evidence of a new $\Xi_{c}(2880)^{0}$ state is found with a local significance of $3.8\,\sigma$, whose mass and width are measured to be $2881.8 \pm 3.1 \pm 8.5\,\mathrm{MeV}$ and $12.4 \pm 5.3 \pm 5.8 \,\mathrm{MeV}$, respectively. In addition, evidence of a new decay mode $\Xi_{c}(2790)^{0} \to \Lambda_{c}^{+} K^{-}$ is found with a significance of $3.7\,\sigma$. The relative branching fraction of $B^{-} \to \Lambda_{c}^{+} \bar{\Lambda}_{c}^{-} K^{-}$ with respect to the $B^{-} \to D^{+} D^{-} K^{-}$ decay is measured to be $2.36 \pm 0.11 \pm 0.22 \pm 0.25$, where the first uncertainty is statistical, the second systematic and the third originates from the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb public pages

Measurement of the ratios of branching fractions R(D∗)\mathcal{R}(D^{*}) and R(D0)\mathcal{R}(D^{0})

The ratios of branching fractions $\mathcal{R}(D^{*})\equiv\mathcal{B}(\bar{B}\to D^{*}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(\bar{B}\to D^{*}\mu^{-}\bar{\nu}_{\mu})$ and $\mathcal{R}(D^{0})\equiv\mathcal{B}(B^{-}\to D^{0}\tau^{-}\bar{\nu}_{\tau})/\mathcal{B}(B^{-}\to D^{0}\mu^{-}\bar{\nu}_{\mu})$ are measured, assuming isospin symmetry, using a sample of proton-proton collision data corresponding to 3.0 fb${ }^{-1}$ of integrated luminosity recorded by the LHCb experiment during 2011 and 2012. The tau lepton is identified in the decay mode $\tau^{-}\to\mu^{-}\nu_{\tau}\bar{\nu}_{\mu}$. The measured values are $\mathcal{R}(D^{*})=0.281\pm0.018\pm0.024$ and $\mathcal{R}(D^{0})=0.441\pm0.060\pm0.066$, where the first uncertainty is statistical and the second is systematic. The correlation between these measurements is $\rho=-0.43$. Results are consistent with the current average of these quantities and are at a combined 1.9 standard deviations from the predictions based on lepton flavor universality in the Standard Model.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-039.html (LHCb public pages