The eCommentary Machine Project

The eCommentary Machine web application ("eComma") enables groups of students, scholars, or general readers to build collaborative commentaries on a text and to search, display, and share those commentaries online

Somatic piRNAs and Transposons are Differentially Regulated During Skeletal Muscle Atrophy and Programmed Cell Death [preprint]

PiWi-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, are ~27 nt long, map antisense to transposons, are oxidation resistant, exhibit a uridine bias at their first nucleotide, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 20 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed when cells become committed to undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is associated with the targeted repression of several retrotransposons and the induction of specific DNA transposons. The developmental changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death

Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death

PIWI-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, ∼27 nt long, map antisense to transposons, are oxidation resistant, exhibit a 5’ uridine bias, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 19 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed coincident with the commitment of the muscles undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is correlated with the repression of several retrotransposons and the induction of specific DNA transposons. The developmentally-regulated changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death

Youth sport during the COVID-19 pandemic: The influence of race and affluence on parents’ perspectives of youth participation

COVID-19 restrictions led to a nearly 50% decline in youth sport participation across the United States (Dorsch et al., 2021). Today, communities and sport organizations have resumed sport. However, research has yet to fully elucidate how COVID-19 restrictions impacted youth participation across different sociodemographic groups during the pandemic. The present study explored the association between race and affluence and their relationship with parents' attitudes toward children's youth sport participation before, during, and after the COVID-19 pandemic. Online questionnaires were completed by a nationally representative sample of 3706 parents (Mage = 39.57 ± 9.03 years) who had a child regularly participating in youth sport before COVID-19. Multivariate Analyses of Variance using Tukey post-hoc tests were conducted to examine the main effect differences by race and affluence and the interactive effects of race and affluence. Results suggest that race and affluence -- independently and in combination -- were salient categorical variables of children's weekly hours of sport participation during the COVID-19 pandemic. Findings highlight that White*high affluent groups had the highest rates of sport participation during the COVID-19 pandemic and that these families desire to invest more time and money at greater rates upon returning. Theoretically, designed intersectionality research is recommended to explore further effects of race and affluence in youth sport

Constitutive Activation of Kappa Opioid Receptors at Ventral Tegmental Area Inhibitory Synapses Following Acute Stress.

Stressful experiences potently activate kappa opioid receptors (κORs). κORs in the ventral tegmental area regulate multiple aspects of dopaminergic and non-dopaminergic cell function. Here we show that at GABAergic synapses on rat VTA dopamine neurons, a single exposure to a brief cold-water swim stress induces prolonged activation of κORs. This is mediated by activation of the receptor during the stressor followed by a persistent, ligand-independent constitutive activation of the κOR itself. This lasting change in function is not seen at κORs at neighboring excitatory synapses, suggesting distinct time courses and mechanisms of regulation of different subsets of κORs. We also provide evidence that constitutive activity of κORs governs the prolonged reinstatement to cocaine-seeking observed after cold water swim stress. Together, our studies indicate that stress-induced constitutive activation is a novel mechanism of κOR regulation that plays a critical role in reinstatement of drug seeking

Coupled land use and ecological models reveal emergence and feedbacks in socio-ecological systems

Acknowledgements: This work was supported by an EPSRC Doctoral Training Centre grant (EP/G03690X/1). Supplementary material (Appendix ECOG‐04039 at ). Appendix 1.Peer reviewedPublisher PD

Nutrient Use and Management Practices on United States Golf Courses

Nutrient use on United States golf courses increases management costs and has the potential to influence ecosystems. Therefore, it is critical to assess nutrient use and management practices to develop and teach best management practices. The objectives of this survey were to measure nutrient use and management practices on United States golf courses in 2021, and to determine if changes occurred since 2006. A survey was developed and distributed via e-mail to 14,033 United States golf facilities, with 1444 responding. From 2006 to 2021, the total projected nitrogen (N), available phosphorus (P2O5), and soluble potash (K2O) applied declined by 41%, 59%, and 54%, to 54,376, 13,761, and 41,386 tons, respectively. These reductions were attributed to course closures, reduced fertilized acres, reduced application rates, and nutrient use restrictions. The percentage of facilities that did not apply P2O5 increased to 21%, which is likely a result of P2O5 application restrictions. Soil testing was associated with greater application rates of N, P2O5, and K2O. Returning clippings, using precision fertilizer applications, reducing turfgrass acreage, and considering N release from soil organic matter were associated with reduced application rates of P2O5. Golf course superintendents have contributed to nationwide reductions in N, P2O5, and K2O, as evidenced by the reduction in fertilized acres and the reduction in nutrient use rates from 2006 to 2021

Survey of Pest Management Practices on US Golf Courses

Integrated pest management (IPM) is an important component of golf course maintenance and includes conventional chemical pesticide use as well as nonchemical cultural management practices. Determining how frequent pest management practices are used on golf courses is critical when developing educational and outreach programs. The objective of this study was to determine the frequency of pest management practices and pesticide mixing and storage facilities on US golf courses. A survey was sent to 14,033 operational US golf facilities with 10% responding. Reliance on all conventional chemical pesticides increased from 2015 to 2021. The reliance on biological control products declined to 14% and reliance on the nonpesticide practice of using plant growth regulators remained equivalent to 2015. The most common pest management practices included monitoring weather patterns and scouting for pests, with 93% of golf facilities reporting the use of both. The use of written IPM and pesticide application plans increased from 44% to 63% of golf facilities between 2015 and 2021, respectively. Generally, mixing and storage facilities remained unchanged from 2015 to 2021. US golf facilities continue to use nonchemical pest management practices, but reliance on chemical pesticides has increased

Fiction Fix 07

https://digitalcommons.unf.edu/fiction_fix/1006/thumbnail.jp

Physician decision making in selection of second-line treatments in immune thrombocytopenia in children.

Immune thrombocytopenia (ITP) is an acquired autoimmune bleeding disorder which presents with isolated thrombocytopenia and risk of hemorrhage. While most children with ITP promptly recover with or without drug therapy, ITP is persistent or chronic in others. When needed, how to select second-line therapies is not clear. ICON1, conducted within the Pediatric ITP Consortium of North America (ICON), is a prospective, observational, longitudinal cohort study of 120 children from 21 centers starting second-line treatments for ITP which examined treatment decisions. Treating physicians reported reasons for selecting therapies, ranking the top three. In a propensity weighted model, the most important factors were patient/parental preference (53%) and treatment-related factors: side effect profile (58%), long-term toxicity (54%), ease of administration (46%), possibility of remission (45%), and perceived efficacy (30%). Physician, health system, and clinical factors rarely influenced decision-making. Patient/parent preferences were selected as reasons more often in chronic ITP (85.7%) than in newly diagnosed (0%) or persistent ITP (14.3%, P = .003). Splenectomy and rituximab were chosen for the possibility of inducing long-term remission (P < .001). Oral agents, such as eltrombopag and immunosuppressants, were chosen for ease of administration and expected adherence (P < .001). Physicians chose rituximab in patients with lower expected adherence (P = .017). Treatment choice showed some physician and treatment center bias. This study illustrates the complexity and many factors involved in decision-making in selecting second-line ITP treatments, given the absence of comparative trials. It highlights shared decision-making and the need for well-conducted, comparative effectiveness studies to allow for informed discussion between patients and clinicians