Carbon nanotubes for electrochemical (bio)sensing

Descripció del recurs: el 28 de Gener de 2010El progreso de la nanociencia y la nanotecnología está haciendo posible generar nuevos materiales basados en estructuras de carbono con propiedades únicas y con numerosas aplicaciones tecnológicas. Entre estas aplicaciones se encuentra la mejora de los biosensores, dispositivos capaces de realizar análisis químicos con gran rapidez. Las propiedades mecánicas y eléctricas extraordinarias de los nanotubos de carbono han estimulado extensamente su investigación a lo largo de todo el mundo desde su descubrimiento por Sumio Iijima en 1991. En esta tesis, el estudio del comportamiento electrocatalítico de nanotubos de carbono y al mismo tiempo el diseño de nuevos (bio)sensores electroquímicos han sido el principal objetivo, haciendo uso de diferentes alternativas de integración dentro de los sistemas de (bio)detección, basadas en modificaciones de las superficies del electrodo con nanotubos de carbono, o en el uso de nanotubos de carbono basados en (bio)compositos

Breezy in the Sky with Diamonds

Student generated description: “What a long strange trip 2020 has been. A painting documenting my emotions

Biosensors ecològics

Si a principis de segle XX, els miners utilitzaven canaris tancats en gàbies per a detectar la presència de gasos letals, la química del segle XXI preserva la vida animal i dóna un pas de gegant en la detecció electroquímica. Investigadors de la Universitat Autònoma de Barcelona estudien la possibilitat d'utilitzar un nou biosensor per controlar la presència de fenol al medi ambient. Es tracta d'una substància manufacturada molt present en el camp de la farmacèutica o la clínica, entre d'altres, que genera nivells de toxicitat preocupants. Per poder mesurar aquests nivells, el nou biosensor utilitza com a component biològic un catalitzador fenòlic: la tirosinasa. Un enzim que millora la seva transferència electrònica amb els electrodes del biosensor a través de nanotubs de carboni. De moment, aquests electrodes modificats presenten avantatges electroquímiques que podrien incentivar la producció a gran escala d'aquests biosensors.Si a principios de siglo XX, los mineros utilizaban canarios encerrados en jaulas para detectar la presencia de gases letales, la química del siglo XXI preserva la vida animal y da un paso de gigante en la detección electroquímica. Investigadores de la Universitat Autònoma de Barcelona estudian la posibilidad de utilizar un nuevo biosensor para controlar la presencia de fenol en el medio ambiente. Se trata de una sustancia manufacturada muy presente en el campo de la farmacéutica o la clínica, entre otros, que genera niveles de toxicidad preocupantes. Para poder medir estos niveles, el nuevo biosensor utiliza como componente biológico un catalizador fenólico: la tirosinasa. Una enzima que mejora su transferencia electrónica con los electrodos del biosensor a través de nanotubos de carbono. De momento, estos electrodos modificados presentan ventajas electroquímicas que podrían incentivar la producción a gran escala de estos biosensores.At the beginning of the 20th century miners used caged canaries to detect the presence of lethal gases, but the chemistry of the 21st century respects animals and represents a giant step in electrochemical detection. Researchers at Universitat Autònoma de Barcelona are studying the possibility of using a new biosensor to control the presence of phenol in the environment. Phenol is a manufactured substance widely used in different fields including the clinical and pharmaceutical sectors and generates alarming levels of toxicity. In order to be able to measure these levels, the new biosensor uses a biological component, the enzyme tyrosinase, as a phenol catalyst. An enzyme which improves its electronic transfer with biosensor electrodes through carbon nanotubes. For the moment, these modified electrodes offer electrochemical advantages which could lead to the large scale production of these biosensors

Molecular and immunological characterization of profilin from mugwort pollen

In late summer in Europe, pollen of mugwort is one of the major sources of atopic allergens. No information about the complete molecular structure of any mugwort allergen has been published so far. Here we report the isolation and characterization of mugwort pollen cDNA clones coding for two isoforms of the panallergen profilin. Thirtysix percent of the mugwort allergic patients tested displayed IgE antibodies against natural and recombinant profilin, and no significant differences were observed in the IgEbinding properties of the isoforms. One profilin isoform was purified to homogeneity and detailed structural analysis indicated that the protein exists in solution as dimers and tetramers stabilized by sulfydryl and/or ionic interactions. Profilin monomers were detectable only after exposure of multimers to harsh denaturing conditions. Dimers and tetramers did not significantly differ in their ability to bind serum IgE from mugwort pollenallergic patients. However, oligomeric forms might have a higher allergenic potential than monomers because larger molecules would have additional epitopes for IgEmediated histamine release. Profilin isolated from mugwort pollen also formed multimers. Thus, oligomerization is not an artifact resulting from the recombinant production of the allergen. Inhibition experiments showed extensive IgE crossreactivity of recombinant mugwort profilin and profilin from various pollen and food extracts

The impact of MRP on a traditional manufacturing company

Thesis (M.S.)--Massachusetts Institute of Technology, Sloan School of Management, 1990.Includes bibliographical references (leaves 66-67).by Maria Carolina Briza Junqueira.M.S

ANALISIS STRATEGI DAN UPAYA PEMERINTAH YANG INOVATIF UNTUK MEWUJUDKAN PROGRAM DESA UNGGUL DAN KEBERLANJUTAN

The people in Indonesia have the right to obtain the same facilities and infrastructure and have the obligation to take part in the people's welfare efforts organized by the government. This is one of the government's policies in the development of social welfare. In essence, the basic needs of the Indonesian people such as clothing, food, housing, health, and education must be met. To obtain all of this, the guarantee of comfort and security in an area is very necessary and is the main factor for the continuity of comfortable and safe village life. However, seeing the different conditions of every human being, some of them do not have public awareness in protecting and maintaining the surrounding environment. This requires us as fellow human beings to create an effort to restore public awareness of the environment we live in and its surroundings. Based on the problems above, this has led to speculation that the government is not paying attention to improving environmental welfare so that this problem will not go away. This study aims to determine the role of the government in supporting the level of public awareness of the welfare of the surrounding environment and to show the impact that occurs. This research approach is carried out with qualitative research methods, then the data used is secondary data that the authors get from the official website of the Ministry of Health.go.id as well as government report documents or previous journals. Data were analyzed using Nvivo 12 plus. The results of the study indicate that the efforts made by the village government to increase awareness of environmental welfare in rural communities by collecting village potential or available resources in the village. In addition to the village community, the government, the private sector, and activists also play a very important role in efforts to increase environmental health awareness and this research is carried out to form a superior and sustainable village. The limitation of this research is that the data used is still very limited.Masyarakat di Indonesia berhak untuk memperoleh fasilitas sarana dan prasarana yang sama dan memiliki kewajiban untuk ikut andil dalam usaha kesejahteraan rakyat yang diselenggarakan oleh pemerintah. Hal ini merupakan salah satu kebijaksanaan pemerintah dalam pembangunan di bidang kesejahteraan sosial. Pada hakekatnya, kebutuhan pokok masyarakat Indonesia seperti sandang, pangan, papan, kesehatan, dan pendidikan harus terpenuhi. Untuk memperoleh itu semua, jaminan kenyamanan dan keamanan dalam suatu wilayah sangat diperlukan dan merupakan faktor utama untuk keberlangsungan kehidupan desa yang nyaman dan aman. Namun, melihat kondisi setiap manusia yang berbeda, membuat beberapa dari mereka tidak memiliki kesadaran masyarakat dalam menjaga dan memelihara lingkungan di sekitarnya. Hal ini menuntut kita sebagai sesama umat manusia untuk menciptakan sebuah upaya pengembalian kesadaran masyarakat terhadap lingkungan tempat tinggal dan sekitarnya. Berdasarkan permasalahan di atas, hal tersebut membuat spekulasi bahwa pemerintah dirasa kurang memperhatikan dalam peningkatan kesejahteraan lingkungan sehingga masalah ini tak kunjung usai. Penelitian ini memiliki tujuan untuk mengetahui peranan pemerintah dalam menunjang tingkat kesadaran masyarakat terhadap kesejahteraan lingkungan disekitarnya serta memperlihatkan dampak yang terjadi. Pendekatan penelitian ini dilakukan dengan metode penelitian kualitatif, lalu data yang digunakan ialah data sekunder yang penulis dapatkan dari website resmi kemendes.go.id serta dokumen laporan pemerintah atau jurnal terdahulu. Data dianalisis menggunakan Nvivo 12 plus. Hasil penelitian menunjukkan bahwa upaya yang dilakukan pemerintah desa guna meningkatkan kesadaran akan kesejahteraan lingkungan pada masyarakat desa dengan cara menghimpun potensi desa atau sumber daya yang tersedia di dalam desa. Selain masyarakat desa, pemerintah, swasta, maupun para aktivis sangat berperan dalam upaya peningkatan kesadaran kesejahteraan lingkungan dan penelinitan ini dilakukan guna membentuk sebuah desa yang unggul dan keberlanjutan. Keterbatasan dalam penelitian ini ialah data yang digunakan masih sangat terbatas

Uma abordagem bayesiana sobre subscrição de riscos de seguro em automóvel através da aplicação do modelo de fronteira estocástica

Mediante uma conjuntura nacional e internacional de incerteza econômica, o papel da gestão de riscos se torna mais relevante. No segmento de seguro, o ramo de automóveis tem uma parcela expressiva no mercado brasileiro, e as empresas podem não estar sendo eficientes. Este trabalho visa a mensuração da ineficiência da subscrição de riscos desse ramo, atividade que se torna parte da estratégia empresarial no atual contexto, medindo o quão distante as seguradoras operam da fronteira de eficiência ideal. Para tal, utiliza-se a inferência bayesiana no modelo de fronteira de produção estocástica

Correlation of sensitizing capacity and T-cell recognition within the Bet v 1 family

BackgroundBet v 1 is the main sensitizing allergen in birch pollen. Like many other major allergens, it contains an immunodominant T cell–activating region (Bet v 1142-156). Api g 1, the Bet v 1 homolog in celery, lacks the ability to sensitize and is devoid of major T-cell epitopes.ObjectiveWe analyzed the T-cell epitopes of Mal d 1, the nonsensitizing Bet v 1 homolog in apple, and assessed possible differences in uptake and antigen processing of Bet v 1, Api g 1, and Mal d 1.MethodsFor epitope mapping, Mal d 1–specific T-cell lines were stimulated with overlapping synthetic 12-mer peptides. The surface binding, internalization, and intracellular degradation of Bet v 1, Api g 1, and Mal d 1 by antigen-presenting cells were compared by using flow cytometry. All proteins were digested with endolysosomal extracts, and the resulting peptides were identified by means of mass spectrometry. The binding of Bet v 1142-156 and the homologous region in Mal d 1 by HLA class II molecules was analyzed in silico.ResultsLike Api g 1, Mal d 1 lacked dominant T-cell epitopes. The degree of surface binding and the kinetics of uptake and endolysosomal degradation of Bet v 1, Api g 1, and Mal d 1 were comparable. Endolysosomal degradation of Bet v 1 and Mal d 1 resulted in very similar fragments. The Bet v 1142-156 and Mal d 1141-155 regions showed no striking difference in their binding affinities to the most frequent HLA-DR alleles.ConclusionThe sensitizing activity of different Bet v 1 homologs correlates with the presence of immunodominant T-cell epitopes. However, the presence of Bet v 1142-156 is not conferred by differential antigen processing

Resiliencia y desempeño laboral en teleoperadores de un call center Lima Metropolitana

El objetivo del estudio fue conocer en qué medida la resiliencia explica el desempeño laboral en los teleoperadores de un call center de Lima metropolitana, 2022. Para ello se usó una metodología de tipo básica y de diseño no experimental y explicativo. Los instrumentos que se utilizaron fueron el cuestionario de resiliencia y el cuestionario de desempeño laboral. Los participantes fueron 500 trabajadores entre los 18 a 50 años de edad. Por otro lado, se hallaron resultados que conllevaron a la aceptación de la hipótesis, debido a que, se determinó una explicación significativa de tipo positiva donde la resiliencia explica moderadamente al desempeño laboral (β = .61; p = .000, Vexplicada = .38). Asimismo, se identificaron influencias bajas de la confianza en sí mismo, perseverancia y satisfacción personal hacia el desempeño laboral (β = .16; β = .11; β = .24). No obstante, no se hallaron influencias de la ecuanimidad y el sentirse bien solo hacia el desempeño laboral (p > .05). En conclusión, la resiliencia ejerce una influencia moderada hacia el desempeño laboral en los teleoperadores de un call center de Lima metropolitana, 2022