10 research outputs found
Improving dietary glucosinolate production, processing and characterization of potential health effects for the prevention of colon cancer
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Even free radicals should follow some rules: a guide to free radical research terminology and methodology.
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species
Selenium, Selenoenzymes, Oxidative Stress and Risk of Neoplastic Progression from Barrett's Esophagus: Results from Biomarkers and Genetic Variants
Clinical trials have suggested a protective effect of selenium supplementation on the risk of esophageal cancer, which may be mediated through the antioxidant activity of selenoenzymes. We investigated whether serum selenium concentrations, selenoenzyme activity, oxidative stress and genetic variation in selenoenzymes were associated with the risk of neoplastic progression to esophageal adenocarcinoma (EA) and two intermediate endpoints, aneuploidy and tetraploidy. In this prospective cohort study, during an average follow-up of 7.3 years, 47 EA cases, 41 aneuploidy cases and 51 tetraploidy cases accrued among 361 participants from the Seattle Barrett's Esophagus Research Study who were free of EA at the time of blood draw and had at least one follow-up visit. Development to EA was assessed histologically and aneuploidy and tetraploidy by DNA content flow cytometry. Serum selenium concentrations were measured using atomic absorption spectrometry, activity of glutathione peroxidase (GPX) 1 and GPX3 by substrate-specific coupled test procedures, selenoprotein P (SEPP1) concentrations and protein carbonyl content by ELISA method and malondialdehyde concentrations by HPLC. Genetic variants in GPX1-4 and SEPP1 were genotyped. Serum selenium was not associated with the risk of neoplastic progression to EA, aneuploidy or tetraploidy (P for trend = 0.25 to 0.85). SEPP1 concentrations were positively associated with the risk of EA [hazard ratio (HR) = 3.95, 95% confidence intervals (CI) = 1.42–10.97 comparing the third tertile with the first] and with aneuploidy (HR = 6.53, 95% CI = 1.31–32.58), but not selenoenzyme activity or oxidative stress markers. No genetic variants, overall, were associated with the risk of neoplastic progression to EA (global p = 0.12–0.69). Our results do not support a protective effect of selenium on risk of neoplastic progression to EA. Our study is the first to report positive associations of plasma SEPP1 concentrations with the risk of EA and aneuploidy, which warrants further investigation
Evidence for a vitamin E-induced gene expression by direct binding to the pregnane X receptor
TrxR1 and Gpx2 are potently induced by isothiocyanates and selenium, and mutually cooperate to protect Caco-2 cells against free radical-mediated cell death
AbstractCurrently, there is significant interest in the field of diet–gene interactions and the mechanisms by which food compounds regulate gene expression to modify cancer susceptibility. From a nutrition perspective, two key components potentially exert cancer chemopreventive effects: isothiocyanates (ITCs), present in cruciferous vegetables, and selenium (Se) which, as selenocysteine, is an integral part of selenoproteins. However, the role of these compounds in the expression of key selenoenzymes once the cancer process has been initiated still needs elucidation. Therefore, this investigation examined the effect of two forms of selenium, selenium-methylselenocysteine and sodium selenite, both individually and in combination with two ITCs, sulforaphane or iberin, on the expression of the two selenoenzymes, thioredoxin reductase 1 (TrxR1) and gastrointestinal glutathione peroxidase (GPx2), which are targets of ITCs, in Caco-2 cells. Co-treatment with both ITCs and Se induced expression of TrxR1 and GPx2 more than either compound alone. Moreover, pre-treatment of cells with ITC+Se enhanced cytoprotection against H2O2-induced cell death through a ROS-dependent mechanism. Furthermore, a single and double knockdown of TrxR1 and/or GPx2 suggested that both selenoproteins were responsible for protecting against H2O2-induced cell death. Together, these data shed new light on the mechanism of interactions between ITC and Se in which translational expression of the enhanced transcripts by the former is dependent on an adequate Se supply, resulting in a cooperative antioxidant protective effect against cell death
Tumor necrosis factor-induced apoptosis during the poisoning of mice with hepatotoxins
BACKGROUND & AIMS: Treatment with tumor necrosis factor (TNF) induces murine hepatocyte apoptosis in vitro and in vivo when sensitizing concentrations of toxins are present. The aim of this study was to investigate whether endogenously formed TNF contributes to liver failure caused by hepatotoxins. METHODS: The extent of liver damage, induced by alpha-amanitin or actinomycin D (ActD), was examined under various experimental conditions, preventing the action of TNF on hepatocytes. RESULTS: TNF induced apoptosis of murine hepatocytes or human hepatoma cells in the presence of alpha-amanitin or ActD. TNF and alpha-amanitin induced such hepatotoxicity also in vivo in a synergistic way. After in vivo administration of high doses of ActD or alpha-amanitin alone, hepatic TNF-messenger RNA was increased and hepatocytes underwent apoptosis. A neutralizing antiserum against TNF- alpha prevented the liver injury. Hepatotoxicity of ActD or alpha- amanitin also was prevented by pretreatment of mice with low doses of the tolerizing cytokine interleukin 1. Mice deficient for the 55- kilodalton TNF receptor were protected from ActD- or alpha-amanitin- induced toxicity. Endotoxin-unresponsive C3H/HeJ mice also had liver failure after ActD treatment, and this damage was prevented by treatment with anti-TNF antiserum. CONCLUSIONS: Hepatotoxins such as alpha-amanitin may induce liver failure by an indirect mechanism involving sensitization of parenchymal cells toward endogenously produced TNF
Long-term Effects of vitamin E, Vitamin C, and Combined Supplementation on Urinary 7,8-dihydro-Oxodeoxyguanosine, serum Cholesterol Oxidation Products and Oxidation Resistance of Lipids in Non-depleted Men
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Even free radicals should follow some rules: a guide to free radical research terminology and methodology.
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species