27 research outputs found
Human G ProteinâCoupled Receptor Gpr-9-6/Cc Chemokine Receptor 9 Is Selectively Expressed on Intestinal Homing T Lymphocytes, Mucosal Lymphocytes, and Thymocytes and Is Required for Thymus-Expressed ChemokineâMediated Chemotaxis
TECK (thymus-expressed chemokine), a recently described CC chemokine expressed in thymus and small intestine, was found to mediate chemotaxis of human G proteinâcoupled receptor GPR-9-6/L1.2 transfectants. This activity was blocked by antiâGPR-9-6 monoclonal antibody (mAb) 3C3. GPR-9-6 is expressed on a subset of memory α4ÎČ7high intestinal trafficking CD4 and CD8 lymphocytes. In addition, all intestinal lamina propria and intraepithelial lymphocytes express GPR-9-6. In contrast, GPR-9-6 is not displayed on cutaneous lymphocyte antigenâpositive (CLA+) memory CD4 and CD8 lymphocytes, which traffic to skin inflammatory sites, or on other systemic α4ÎČ7âCLAâ memory CD4/CD8 lymphocytes. The majority of thymocytes also express GPR-9-6, but natural killer cells, monocytes, eosinophils, basophils, and neutrophils are GPR-9-6 negative. Transcripts of GPR-9-6 and TECK are present in both small intestine and thymus. Importantly, the expression profile of GPR-9-6 correlates with migration to TECK of blood T lymphocytes and thymocytes. As migration of these cells is blocked by antiâGPR-9-6 mAb 3C3, we conclude that GPR-9-6 is the principal chemokine receptor for TECK. In agreement with the nomenclature rules for chemokine receptors, we propose the designation CCR-9 for GPR-9-6. The selective expression of TECK and GPR-9-6 in thymus and small intestine implies a dual role for GPR-9-6/CCR-9, both in T cell development and the mucosal immune response
Utilizing the great blue heron (Ardea herodias) in ecological risk assessments of bioaccumulative contaminants
Human G ProteinâCoupled Receptor Gpr-9-6/Cc Chemokine Receptor 9 Is Selectively Expressed on Intestinal Homing T Lymphocytes, Mucosal Lymphocytes, and Thymocytes and Is Required for Thymus-Expressed ChemokineâMediated Chemotaxis
The conformational dynamics and subunit equilibrium of Escherichia coli ribosomal protein L7/L12
Thesis (Ph. D.)--University of Hawaii at Manoa, 1994.Includes bibliographical references (l. 279-294)Microfiche.xxi, 294 leaves, bound illus. 29 c
A Homogeneous G Protein-Coupled Receptor Ligand Binding Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer
Identification of a Membrane-Spanning Domain of the Thiol-Activated Pore-Forming Toxin Clostridium perfringens
BiP Maintains the Permeability Barrier of the ER Membrane by Sealing the Lumenal End of the Translocon Pore before and Early in Translocation
Discovery of a Type III Inhibitor of LIM Kinase 2 That Binds in a DFG-Out Conformation
The first allosteric, type III inhibitor
of LIM-kinase 2 (LIMK2)
is reported. A series of molecules that feature both an <i>N</i>-phenylsulfonamide and tertiary amide were not only very potent at
LIMK2 but also were extremely selective against a panel of other kinases.
Enzymatic kinetic studies showed these molecules to be noncompetitive
with ATP, suggesting allosteric inhibition. X-ray crystallography
confirmed that these sulfonamides are a rare example of a type III
kinase inhibitor that binds away from the highly conserved hinge region
and instead resides in the hydrophobic pocket formed in the DFG-out
conformation of the kinase, thus accounting for the high level of
selectivity observed