Activation of the Listeria monocytogenes Virulence Program by a Reducing Environment.

Upon entry into the host cell cytosol, the facultative intracellular pathogen Listeria monocytogenes coordinates the expression of numerous essential virulence factors by allosteric binding of glutathione (GSH) to the Crp-Fnr family transcriptional regulator PrfA. Here, we report that robust virulence gene expression can be recapitulated by growing bacteria in a synthetic medium containing GSH or other chemical reducing agents. Bacteria grown under these conditions were 45-fold more virulent in an acute murine infection model and conferred greater immunity to a subsequent lethal challenge than bacteria grown in conventional media. During cultivation in vitro, PrfA activation was completely dependent on the intracellular levels of GSH, as a glutathione synthase mutant (ΔgshF) was activated by exogenous GSH but not reducing agents. PrfA activation was repressed in a synthetic medium supplemented with oligopeptides, but the repression was relieved by stimulation of the stringent response. These data suggest that cytosolic L. monocytogenes interprets a combination of metabolic and redox cues as a signal to initiate robust virulence gene expression in vivoIMPORTANCE Intracellular pathogens are responsible for much of the worldwide morbidity and mortality from infectious diseases. These pathogens have evolved various strategies to proliferate within individual cells of the host and avoid the host immune response. Through cellular invasion or the use of specialized secretion machinery, all intracellular pathogens must access the host cell cytosol to establish their replicative niches. Determining how these pathogens sense and respond to the intracellular compartment to establish a successful infection is critical to our basic understanding of the pathogenesis of each organism and for the rational design of therapeutic interventions. Listeria monocytogenes is a model intracellular pathogen with robust in vitro and in vivo infection models. Studies of the host-sensing and downstream signaling mechanisms evolved by L. monocytogenes often describe themes of pathogenesis that are broadly applicable to less tractable pathogens. Here, we describe how bacteria use external redox states as a cue to activate virulence

Characterization of the initial filamentation of a relativistic electron beam passing through a plasma

The linear instability that induces a relativistic electron beam passing through a return plasma current to filament transversely is often related to some filamentation mode with wave vector normal to the beam or confused with Weibel modes. We show that these modes may not be relevant in this matter and identify the most unstable mode on the two-stream/filamentation branch as the main trigger for filamentation. This sets both the characteristic transverse and longitudinal filamentation scales in the non-resistive initial stage.Comment: 4 page, 3 figures, to appear in PR

Instabilities for a relativistic electron beam interacting with a laser irradiated plasma

The effects of a radiation field (RF) on the unstable modes developed in relativistic electron beam--plasma interaction are investigated assuming that $\omega_{0} >\omega_{p}$, where $\omega_{0}$ is the frequency of the RF and $\omega_{p}$ is the plasma frequency. These unstable modes are parametrically coupled to each other due to the RF and are a mix between two--stream and parametric instabilities. The dispersion equations are derived by the linearization of the kinetic equations for a beam--plasma system as well as the Maxwell equations. In order to highlight the effect of the radiation field we present a comparison of our analytical and numerical results obtained for nonzero RF with those for vanishing RF. Assuming that the drift velocity $\mathbf{u}_{b}$ of the beam is parallel to the wave vector $\mathbf{k}$ of the excitations two particular transversal and parallel configurations of the polarization vector $\mathbf{E}_{0}$ of the RF with respect to $\mathbf{k}$ are considered in detail. It is shown that in both geometries resonant and nonresonant couplings between different modes are possible. The largest growth rates are expected at the transversal configuration when $\mathbf{E}_{0}$ is perpendicular to $\mathbf{k}$. In this case it is demonstrated that in general the spectrum of the unstable modes in $\omega$--$k$ plane is split into two distinct domains with long and short wavelengths, where the unstable modes are mainly sensitive to the beam or the RF parameters, respectively. In parallel configuration, $\mathbf{E}_{0} \parallel \mathbf{k}$, and at short wavelengths the growth rates of the unstable modes are sensitive to both beam and RF parameters remaining insensitive to the RF at long wavelengths.Comment: 23 pages, 5 figure

Screening of Spherical Colloids beyond Mean Field -- A Local Density Functional Approach

We study the counterion distribution around a spherical macroion and its osmotic pressure in the framework of the recently developed Debye-H"uckel-Hole-Cavity (DHHC) theory. This is a local density functional approach which incorporates correlations into Poisson-Boltzmann theory by adding a free energy correction based on the One Component Plasma. We compare the predictions for ion distribution and osmotic pressure obtained by the full theory and by its zero temperature limit with Monte Carlo simulations. They agree excellently for weakly developed correlations and give the correct trend for stronger ones. In all investigated cases the DHHC theory and its computationally simpler zero temperature limit yield better results than the Poisson-Boltzmann theory.Comment: 10 pages, 4 figures, 2 tables, RevTeX4-styl

Index

The interest in relativistic beam-plasma instabilities has been greatly rejuvenated over the past two decades by novel concepts in laboratory and space plasmas. Recent advances in this long-standing field are here reviewed from both theoretical and numerical points of view. The primary focus is on the two-dimensional spectrum of unstable electromagnetic waves growing within relativistic, unmagnetized, and uniform electron beam-plasma systems. Although the goal is to provide a unified picture of all instability classes at play, emphasis is put on the potentially dominant waves propagating obliquely to the beam direction, which have received little attention over the years. First, the basic derivation of the general dielectric function of a kinetic relativistic plasma is recalled. Next, an overview of two-dimensional unstable spectra associated with various beam-plasma distribution functions is given. Both cold-fluid and kinetic linear theory results are reported, the latter being based on waterbag and Maxwell–Jüttner model distributions. The main properties of the competing modes (developing parallel, transverse, and oblique to the beam) are given, and their respective region of dominance in the system parameter space is explained. Later sections address particle-in-cell numerical simulations and the nonlinear evolution of multidimensional beam-plasma systems. The elementary structures generated by the various instability classes are first discussed in the case of reduced-geometry systems. Validation of linear theory is then illustrated in detail for large-scale systems, as is the multistaged character of the nonlinear phase. Finally, a collection of closely related beam-plasma problems involving additional physical effects is presented, and worthwhile directions of future research are outlined.Original Publication: Antoine Bret, Laurent Gremillet and Mark Eric Dieckmann, Multidimensional electron beam-plasma instabilities in the relativistic regime, 2010, Physics of Plasmas, (17), 12, 120501-1-120501-36. http://dx.doi.org/10.1063/1.3514586 Copyright: American Institute of Physics http://www.aip.org/</p

Neutron Resonance Spectroscopy of 117Sn from1 eV to 1.5 keV

Parity violation has been studied recently for neutron resonances in 117Sn. The neutron resonance spectroscopy is essential for the analysis of the parity violation data. We have measured neutron resonances in 117Sn for neutron energies from 1 to 1500 eV using the time-of-flight method and the (n,γ) reaction. The sample was enriched to 87.6% 117Sn. Neutron scattering and radiative widths were determined, and orbital angular momentum assignments were made with a Bayesian analysis. The s-wave and p-wave strength functions and average level spacings were determined

Parity Violation in Neutron Resonances of 117 Sn

Parity nonconservation (PNC) has been studied in neutron p-wave resonances of 117Sn. The longitudinal asymmetries were measured for 29 p-wave resonances in the neutron energy range 0.8 eV to 1100 eV. Statistically significant PNC effects were observed for four resonances. A statistical analysis determined the rms weak mixing matrix element and the weak spreading width. A weak spreading width of Γw=(0.28-0.15+0.56)×10-7 eV was obtained for117Sn