Induced pluripotent stem cells, a giant leap for mankind therapeutic applications

Induced pluripotent stem cells (iPSC) technology has propelled the field of stem cells biology, providing new cells to explore the molecular mechanisms of pluripotency, cancer biology and aging. A major advantage of human iPSC, compared to the pluripotent embryonic stem cells, is that they can be generated from virtually any embryonic or adult somatic cell type without destruction of human blastocysts. In addition, iPSC can be generated from somatic cells harvested from normal individuals or patients, and used as a cellular tool to unravel mechanisms of human development and to model diseases in a manner not possible before. Besides these fundamental aspects of human biology and physiology that are revealed using iPSC or iPSC-derived cells, these cells hold an immense potential for cell-based therapies, and for the discovery of new or personalized pharmacological treatments for many disorders. Here, we review some of the current challenges and concerns about iPSC technology. We introduce the potential held by iPSC for research and development of novel health-related applications. We briefly present the efforts made by the scientific and clinical communities to create the necessary guidelines and regulations to achieve the highest quality standards in the procedures for iPSC generation, characterization and long-term preservation. Finally, we present some of the audacious and pioneer clinical trials in progress with iPSC-derived cells.info:eu-repo/semantics/publishedVersio

Notes on Governance

Governance is a multi-layered concept that depicts a myriad of formulations and potential affordances. In this paper, we present and discuss some of the key topics that frame the governance problems European Universities face nowadays. We depart from institutions and their interactions with the outer world and their organizational structures and then focus on power and productivity as the two core variables influencing governance. Following the contributions and remarks of several thinkers throughout history, we then reflect on the emergence of governance and the central function it has had throughout history in supporting how institutions survive and prevail sometimes in daring circumstances. We end our notes with an assertion of the plasticity of the concept and its many affordances that go way beyond managerial or political thinking, making governance uncapturable

How effective are brief interventions in smoking cessation: project of a cohort study in a family health care unit

ABSTRACT: Tobacco use remains the most significant modifiable cause of disability, death and illness1. In Portugal, 19,6% of the population aged ten years or more smoke3. A Cochrane review of 20087 concluded that a brief advice intervention (compared to usual care) can increase the likelihood of a smoker to quit and remain nonsmoker 12 months later by a further 1 to 3 %. Several studies have shown that Primary Care Physicians can play a key role in these interventions8,9,10. However we did not find studies about the effectiveness of brief interventions in routine consultations of Family Doctors in Portugal. For this reason we designed a Cohort Study to make an exploratory study about the effectiveness of brief interventions of less than three minutes in comparison with usual care in routine consultations. The study will be implemented in a Family Healthcare Unit in Beja, during six months. Family Doctors of the intervention group should be submitted for an educational and training program before the study begin. Quit smoking sustained rates will be estimated one year after the first intervention in each smoker. If, as we expect, quit smoking rates will be higher in the intervention group than in the control group, this may change Portuguese Family Doctors attitudes and increase the provision of brief interventions in routine consultations in Primary Healthcare Centers

Induced pluripotent stem cell line (INSAi001-A) from a Gaucher disease type 3 patient compound heterozygote for mutations in the GBA1 gene

Gaucher Disease (GD) type 3 is a neurological form of a multisystemic autosomal recessive disorder belonging to the group of lysosomal storage diseases. Causal mutations in the glucocerebrosidase 1 (GBA1) commonly lead to abnormal protein and GD, heterozygosity is a genetic risk factor for Parkinson's disease. This work describes the use of a non-integrative approach using Sendai Virus delivery to establish induced Pluripotent Stem Cells (iPSCs) from fibroblasts from a GD type 3 patient. Differentiation of iPSCs can be employed to generate a variety of complex cell types with a high degree of genetic complexity that would otherwise be unattainable.Funding Agency Portuguese Foundation for Science and Technology PTDC/BIM-MEC/4762/2014info:eu-repo/semantics/publishedVersio

How to address sustainability at the city level

A brief analysis of the environmental, social and economic paradigm of nowadays cities, allows reaching a conclusion: current cities are not sustainable. Considering this, it is very important to study the causes of such unsustainability and to develop models for sustainable cities. There are a limited number of studies in this area, but the scientific community lacks even more studies related to the implementation of these models in current cities. Consequently there is the need to explore this field. This work emerges with the objective of analysing how it is possible to implement sustainability in cities throw the development of a sustainable city model and an urban regeneration plan. The development of sustainable city models is a very complex topic. The analysis of the state of the art shows that one must consider the relation between environmental and social aspects in the development of sustainable cities, while taking special concerns when considering the economic issues. It was also concluded that cities should be subject to performance assessments. It was also demonstrated that a city can only be sustainable if its population behaves in harmony with the city’s sustainability model and that the behavior of a community is determinant in the sustainability level that a model can achieve

Guidelines for analysing the building energy efficiency using BIM

Introduction The benefits of Building Information Modelling (BIM) for the construction industry are already known and proved. One of the applications of BIM is to estimate buildings energy demand and foster the introduction of more efficient design alternatives. BIM creates information to support designers' decision-making and to allow them to compare and select the best options, enhancing buildings energy efficiency. With the increasing use of BIM and energy simulation tools, there is a need to establish a common and valid procedure for buildings energy assessment. Material and Methods By carrying out a building simulation with BIM, this paper identifies and describes the fundamental stages and requirements to perform a reliable, concise and fast energy assessment. These guidelines will consider Autodesk Revit as a BIM platform to create and prepare the building 3D model. Results The proposed guidelines cover all the required tasks for a BIM energy assessment, as the building modulation details and requirements, the software interoperability modes and restrictions and the comparison and selection of improvement measures. Inputs and advice are given regarding the use of some of the existing energy simulation BIM tools. Conclusions With the increasing number of studies addressing energy simulations with BIM, it is essential to define a set of requisites to precisely perform them. By identifying the minimum requirements and tasks, the proposed guidelines will support future studies in achieving more reliable and comparable results. The possibility to estimate and improve the energy demand of a building during the initial project stages allows for the opportunity to build high performance and more sustainable buildings. BIM capabilities might be an essential support to effectively improve the built environment energy efficiency and accomplish environmental goals.FCT -Fundação para a Ciência e a Tecnologia(SFRH/BD/145735/2019

A systematic review of the role of BIM in building sustainability assessment methods

Building Information Modelling (BIM) is creating new opportunities for the Architecture, Engineering and Construction industry. One of them is the integration of the Building Sustainability Assessment (BSA) during the design process. Currently, an approach for using BIM to foster and optimise the application of BSA methods has not been clearly established yet, creating a knowledge gap on the application of BIM for sustainability assessment purposes. Thus, this paper analyses the current role of BIM to evaluate three BSA methods—LEED, BREEAM and SBTool. The current BIM applicability is assessed by performing a systematic review, where the criteria being assessed and the applied BIM software are identified. A comparison is made to determine which BSA method can currently take more advantage from BIM and to identify the number of assessed criteria from each one. Furthermore, the attractiveness of a BIM-based assessment for SBTool is analysed, facing the actual BIM scenario for LEED and BREEAM. Despite the restrictions, BIM use is increasing for sustainability purposes. Most of the analysed studies and identified software are still focused on the use of LEED for assessing sustainability during the design phase. However, BIM software capabilities can also support the assessment of the other BSA methods so that process replicability can happen. Among the most addressed criteria, the energy and material-related categories are the most eminent. Autodesk Revit is the most-used software. A BIM-based assessment for SBTool will have enough attractiveness. It can assess, at least, the same percentage of criteria as the other schemes, creating new opportunities to enhance building sustainability.This research was funded by the Portuguese Foundation for Science and Technology, grant number SFRH/BD/145735/2019