In pursuit of knowledge: Addressing barriers to effective conservation evaluation

Evaluation, the process of assessing the effectiveness of programs and activities, has gained increasing attention in the conservation sector as programs seek to account for investments, measure their impacts, and adapt interventions to improve future outcomes. We conducted a country-wide evaluation of terrestrial-based conservation programs in Samoa. Though rarely applied, the benefit of evaluating multiple projects at once is that it highlights factors which are persistent and influential across the entire conservation sector. We found mixed success in achieving goals among conservation programs; yet this result is surrounded by uncertainty because of the quality of existing evidence on project outcomes. We explore the role of different components of the conservation management system, i.e., context, planning, inputs, processes, and outputs, in facilitating and/or constraining collection of data on project outcomes, and thereby assessment of whether projects were successful. Our study identified a number of direct and indirect barriers that affected the capacity of projects to carry out informative evaluations and generate knowledge on conservation progress in Samoa. These attributes and mechanisms include: the availability and management of data, design and planning of projects, and systems for reporting among donors and proponents. To overcome these barriers to evaluation, we believe that a shift in institutional approaches to reporting outcomes is needed, from a reflective way of thinking to a more prospective outlook

Trends and biases in the listing and recovery planning for threatened species: an Australian case study

Many countries rely on formal legislation to protect and plan for the recovery of threatened species. Even though the listing procedures in threatened species legislation are designed to be consistent for all species there is usually a bias in implementing the laws towards charismatic fauna and flora, which leads to uneven allocation of conservation efforts. However, the extent of bias in national threatened species lists is often unknown. Australia is a good example: the list of threatened species under the Environmental Protection and Biological Conservation Act has not been reviewed since 2000, when it was first introduced. We assessed how well this Act represents threatened species across taxonomic groups and threat status, and whether biases exist in the types of species with recovery plans. We found that birds, amphibians and mammals have high levels of threatened species (12-24%) but < 6% of all reptiles and plants and < 0.01% of invertebrates and fish are considered threatened. Similar taxonomic biases are present in the types of species with recovery plans. Although there have been recent improvements in the representation of threatened species with recovery plans across taxonomic groups, there are still major gaps between the predicted and listed numbers of threatened species. Because of biases in the listing and recovery planning processes many threatened species may receive little attention regardless of their potential for recovery: a lost opportunity to achieve the greatest conservation impact possible. The Environmental Protection and Biological Conservation Act in Australia needs reform to rectify these biases

Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses

Advances in proteomic techniques have allowed the large-scale identification of phosphorylation sites in complex protein samples, but new biological insight requires an understanding of their in vivo dynamics. Here, we demonstrate the use of a stable isotope-based quantitative approach for pathway discovery and structure–function studies in Arabidopsis cells treated with the bacterial elicitor flagellin. The quantitative comparison identifies individual sites on plasma membrane (PM) proteins that undergo rapid phosphorylation or dephosphorylation. The data reveal both divergent dynamics of different sites within one protein and coordinated regulation of homologous sites in related proteins, as found for the PM H+-ATPases AHA1, 2 and 3. Strongly elicitor-responsive phosphorylation sites may reflect direct regulation of protein activity. We confirm this prediction for RbohD, an NADPH oxidase that mediates the rapid production of reactive oxygen species (ROS) in response to elicitors and pathogens. Plant NADPH oxidases are structurally distinct from their mammalian homologues, and regulation of the plant enzymes is poorly understood. On RbohD, we found both unchanging and strongly induced phosphorylation sites. By complementing an RbohD mutant plant with non-phosphorylatable forms of RbohD, we show that only those sites that undergo differential regulation are required for activation of the protein. These experiments demonstrate the potential for use of quantitative phosphoproteomics to determine regulatory mechanisms at the molecular level and provide new insights into innate immune responses

N-methyl-D-aspartate receptors mediate the phosphorylation and desensitization of muscarinic receptors in cerebellar granule neurons.

Changes in synaptic strength mediated by ionotropic glutamate N-methyl-D-asparate (NMDA) receptors is generally considered to be the molecular mechanism underlying memory and learning. NMDA receptors themselves are subject to regulation through signaling pathways that are activated by G-protein-coupled receptors (GPCRs). In this study we investigate the ability of NMDA receptors to regulate the signaling of GPCRs by focusing on the G(q/11)-coupled M(3)-muscarinic receptor expressed endogenously in mouse cerebellar granule neurons. We show that NMDA receptor activation results in the phosphorylation and desensitization of M(3)-muscarinic receptors through a mechanism dependent on NMDA-mediated calcium influx and the activity of calcium-calmodulin-dependent protein kinase II. Our study reveals a complex pattern of regulation where GPCRs (M(3)-muscarinic) and NMDA receptors can feedback on each other in a process that is likely to influence the threshold value of signaling networks involved in synaptic plasticity

A social-ecological approach to conservation planning: embedding social considerations

Many conservation plans remain unimplemented, in part because of insufficient consideration of the social processes that influence conservation decisions. Complementing social considerations with an integrated understanding of the ecology of a region can result in a more complete conservation approach. We suggest that linking conservation planning to a social-ecological systems (SES) framework can lead to a more thorough understanding of human-environment interactions and more effective integration of social considerations. By characterizing SES as a set of subsystems, and their interactions with each other and with external factors, the SES framework can improve our understanding of the linkages between social and ecological influences on the environment. Using this framework can help to identify socially and ecologically focused conservation actions that will benefit ecosystems and human communities, and assist in the development of more consistent evidence for evaluating conservation actions by comparing conservation case studies

Reactivity of a dititanium bis(pentalene) complex toward heteroallenes and main-group element–element bonds

The reactivity of the Ti═Ti double bond in (μ,η5:η5-Pn†)2Ti2 (1; Pn† = 1,4-{SiiPr3}2C8H4) toward isocyanide and heteroallene substrates, and molecules featuring homonuclear bonds between main-group elements (E–E) has been explored. Reaction of 1 with methyl isocyanide or 1,3-N,N′-di-p-tolylcarbodiimide resulted in the formation of the 1:1 adducts (μ,η5:η5-Pn†)2Ti2(μ,η2-CNMe) (2) and (μ,η5:η5-Pn†)2Ti2(μ-C{N(4-C6H4CH3)}2) (3), respectively, which are thermally stable up to 100 °C in contrast to the analogous adducts formed with CO and CO2. Reaction of 1 with phenyl isocyanate afforded a paramagnetic complex, [(η8-Pn†)Ti]2(μ,κ2:κ2-O2CNPh) (4), in which the “double-sandwich” architecture of 1 has been broken and an unusual phenyl-carbonimidate ligand bridges two formally Ti(III) centers. Reaction of 1 with diphenyl dichalcogenides, Ph2E2 (E = S, Se, Te), led to the series of Ti–Ti single-bonded complexes (μ,η5:η5-Pn†)2[Ti(EPh)]2 (E = S (5), Se (6), Te (7)), which can be considered the result of a 2e– redox reaction or a 1,2-addition across the Ti═Ti bond. Treatment of 1 with azobenzene or phenyl azide afforded [(η8-Pn†)Ti]2(μ-NPh)2 (8), a bridging imido complex in which the pentalene ligands bind in an η8 fashion to each formally Ti(IV) center, as the result of a 4e– redox reaction driven by the oxidative cleavage of the Ti═Ti double bond. The new complexes 2–8 were extensively characterized by various techniques including multinuclear NMR spectroscopy and single-crystal X-ray diffraction, and the experimental work was complemented by density functional theory (DFT) studies

Calculating the carbon footprint:implications for governing emissions and gender relations

In this article, we use fresh empirical evidence, and draw on feminist and critical accounting and organisational theories to contend that carbon calculators can be interpreted as discriminatory control technologies. They do this by providing a new and flexible vocabulary for governing expenses, costs and investments at a distance, avoiding a sense of direct intervention by the government. Thus, given our stance that the carbon calculator cannot be considered a neutral tool, we argue that it has the potential to control personal responsibilities regarding both environmental and family‐based issues

Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112.

Mycobacterium tuberculosis (Mtb) is able to persist in the body through months of multi-drug therapy. Mycobacteria possess a wide range of regulatory proteins, including the protein kinase B (PknB) which controls peptidoglycan biosynthesis during growth. Here, we observed that depletion of PknB resulted in specific transcriptional changes that are likely caused by reduced phosphorylation of the H-NS-like regulator Lsr2 at threonine 112. The activity of PknB towards this phosphosite was confirmed with purified proteins, and this site was required for adaptation of Mtb to hypoxic conditions, and growth on solid media. Like H-NS, Lsr2 binds DNA in sequence-dependent and non-specific modes. PknB phosphorylation of Lsr2 reduced DNA binding, measured by fluorescence anisotropy and electrophoretic mobility shift assays, and our NMR structure of phosphomimetic T112D Lsr2 suggests that this may be due to increased dynamics of the DNA-binding domain. Conversely, the phosphoablative T112A Lsr2 had increased binding to certain DNA sites in ChIP-sequencing, and Mtb containing this variant showed transcriptional changes that correspond with the change in DNA binding. In summary, PknB controls Mtb growth and adaptations to the changing host environment by phosphorylating the global transcriptional regulator Lsr2

Spatial variations in lead isotopes, Tasman Element, eastern Australia

Lead isotope data from ore deposits and mineral occurrences in the Tasman Element of eastern Australia have been used to construct isotopic maps of this region. These maps exhibit systematic patterns in parameters derived from isotope ratios. The parameters include μ (238U/204Pb), as calculated using the Cumming and Richards (1975) lead evolution model, and the difference between true age of mineralisation and the Cumming and Richards lead isotope model age of mineralisation (Δt). Variations in μ coincide with boundaries at the orogen, subprovince and zone scales. The boundary between the Lachlan and New England orogens is accompanied by a decrease in μ, and within the Lachlan Orogen, the Central Subprovince is characterised by μ that is significantly higher than in the adjacent Eastern and Western subprovinces. Within the Eastern Subprovince, the Cu-Au-rich Macquarie Arc is characterised by significantly lower μ relative to adjacent rocks. The Macquarie Arc is also characterised by very high Δt (generally above 200 Myr). Other regions characterised by very high Δt include western Tasmania, the southeastern New England Orogen, and the Hodgkinson Province in northern Queensland. These anomalies are within a broad pattern of decreasing Δt from east to west, with Paleozoic deposits within or adjacent to Proterozoic crust characterised by Δt values of 50 Myr or below. The patterns in Δt are interpreted to reflect the presence of the two major tectonic components involved in the Paleozoic Tasman margin in Australia (cf., Münker, 2000): subducting proto-Pacific crust (Δt >150 Myr), and Proterozoic Australia crust (Δt < 50 Myr) on the over-riding plate. Proterozoic Australia crustal sources are interpreted to dominate the western parts of the Tasman Element and Proterozoic crust further to the west, whereas Pacific crustal sources are inferred to characterise western Tasmania and much of the eastern part of the Tasman Element. Contrasts in Δt between the Cambrian Mount Read Volcanics in western Tasmania and similar aged rocks in western Victoria and New South Wales make direct tectonic correlation between these rocks problematic

Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosome segregation in sexual transmission stages

The Aurora family of kinases orchestrates chromosome segregation and cytokinesis during cell division, with precise spatiotemporal regulation of its catalytic activities by distinct protein scaffolds. Plasmodium spp., the causative agents of malaria, are unicellular eukaryotes with three unique and highly divergent aurora-related kinases (ARK1-3) that are essential for asexual cellular proliferation but lack most canonical scaffolds/activators. Here we investigate the role of ARK2 during sexual proliferation of the rodent malaria Plasmodium berghei, using a combination of super-resolution microscopy, mass spectrometry, and live-cell fluorescence imaging. We find that ARK2 is primarily located at spindle microtubules in the vicinity of kinetochores during both mitosis and meiosis. Interactomic and co-localisation studies reveal several putative ARK2-associated interactors including the microtubule-interacting protein EB1, together with MISFIT and Myosin-K, but no conserved eukaryotic scaffold proteins. Gene function studies indicate that ARK2 and EB1 are complementary in driving endomitotic division and thereby parasite transmission through the mosquito. This discovery underlines the flexibility of molecular networks to rewire and drive unconventional mechanisms of chromosome segregation in the malaria parasite.</p