Analyzing the Hemolytic Effects of a Microchannel-Based Blood Processing Device

While hemodialysis is a unique therapy for treating chronic kidney failure, the application of extracorporeal blood processing presents the opportunity for treating a wider range of bloodborne diseases. The objective of the i-Blood research team is to develop a blood processing device that utilizes microscale-based technology as a platform for incorporating novel bioconjugation mechanisms to remove/degrade undesired solutes in the blood or administer drugs. The development of treatment mechanisms for hyperuricemia (high concentrations of uric acid in the blood) and iron overload (high concentrations of iron in the blood) have been the focus for other individuals on the research team. During preliminary development of the device, one concern that came up was the potential for mechanically induced hemolysis due to the foreign flow conditions imposed by the geometry of the device. While red blood cells (RBCs) are especially adept at modifying their shape to endure high pressures in arteries and small orifices in capillaries, the concern remains that the flow through the foreign materials of the device could inflict enough shear stress to break the cell membrane of RBCs. In order to provide a therapeutic benefit, it is essential that the team can identify flow conditions that minimize risks to the patient (i.e. minimized blood damage and coagulation) while simultaneously maintaining the intended functionality of the device for a given disease. The primary objective of this project was to characterize the degree of blood damage in a novel microchannel-based blood processing device by quantifying the hemolytic effects of various flow parameters. A nuanced methodology of measuring the plasma-free hemoglobin concentrations via a hemoglobin detection assay and absorbance spectroscopy was developed. The effects of fluid velocity and number of passes through the device were investigated using a syringe pump apparatus. Experimental results indicated that velocity had an insignificant effect on the hemolytic effects of the device for a single pass while increasing the number of passes at a constant flow rate caused an increase in hemolysis. No specific plate geometry recommendations can be made based on the current results, though recommended next steps and identified concerns for future investigation are discussed in detail. Ultimately, the research project was successful at developing a reliable methodology for quantifying blood damage which can be used throughout the design process

Off-shell effects on particle production

We investigate the observable effects of off-shell propagation of nucleons in heavy-ion collisions at SIS energies. Within a semi-classical BUU transport model we find a strong enhancement of subthreshold particle production when off-shell nucleons are propagated.Comment: 11 pages, 3 figure

Transport Theoretical Approach to the Nucleon Spectral Function in Nuclear Matter

The nucleon spectral function in infinite nuclear matter is calculated in a quantum transport theoretical approach. Exploiting the known relation between collision rates and correlation functions the spectral function is derived self-consistently. By re-inserting the spectral functions into the collision integrals the description of hard processes from the high-momentum components of wave functions and interactions is improved iteratively until convergence is achieved. The momentum and energy distributions and the nuclear matter occupation probabilities are in very good agreement with the results obtained from many-body theory.Comment: minor changes in the text, additional curves in fig.

Spectral Function of Quarks in Quark Matter

We investigate the spectral function of light quarks in infinite quark matter using a simple, albeit self-consistent model. The interactions between the quarks are described by the SU(2) Nambu--Jona-Lasinio model. Currently mean field effects are neglected and all calculations are performed in the chirally restored phase at zero temperature. Relations between correlation functions and collision rates are used to calculate the spectral function in an iterative process.Comment: final version, published in PRC; 15 pages, RevTeX

Baryon flow at SIS energies

We calculate the baryon flow $$ in the energy range from .25 to $\le 2.5 AGeV$ in a relativistic transport model for $Ni+Ni$ and $Au+Au$ collisions employing various models for the baryon self energies. We find that to describe the flow data of the FOPI Collaboration the strength of the vector potential has to be reduced at high relative momentum or at high density such that the Schr\"odinger- equivalent potential at normal nuclear density decreases above 1 GeV relative kinetic energy and approaches zero above 2 GeV.Comment: 20 pages, LATEX, 7 PostScript figure

Towards a fully self-consistent spectral function of the nucleon in nuclear matter

We present a calculation of nuclear matter which goes beyond the usual quasi-particle approximation in that it includes part of the off-shell dependence of the self-energy in the self-consistent solution of the single-particle spectrum. The spectral function is separated in contributions for energies above and below the chemical potential. For holes we approximate the spectral function for energies below the chemical potential by a $\delta$-function at the quasi-particle peak and retain the standard form for energies above the chemical potential. For particles a similar procedure is followed. The approximated spectral function is consistently used at all levels of the calculation. Results for a model calculation are presented, the main conclusion is that although several observables are affected by the inclusion of the continuum contributions the physical consistency of the model does not improve with the improved self-consistency of the solution method. This in contrast to expectations based on the crucial role of self-consistency in the proofs of conservation laws.Comment: 26 pages Revtex with 4 figures, submitted to Phys. Rev.

Fragment Formation in Central Heavy Ion Collisions at Relativistic Energies

We perform a systematic study of the fragmentation path of excited nuclear matter in central heavy ion collisions at the intermediate energy of $0.4 AGeV$. The theoretical calculations are based on a Relativistic Boltzmann-Uehling-Uhlenbeck ($RBUU$) transport equation including stochastic effects. A Relativistic Mean Field ($RMF$) approach is used, based on a non-linear Lagrangian, with coupling constants tuned to reproduce the high density results of calculations with correlations. At variance with the case at Fermi energies, a new fast clusterization mechanism is revealed in the early compression stage of the reaction dynamics. Fragments appear directly produced from phase-space fluctuations due to two-body correlations. In-medium effects of the elastic nucleon-nucleon cross sections on the fragmentation dynamics are particularly discussed. The subsequent evolution of the primordial clusters is treated using a simple phenomenological phase space coalescence algorithm. The reliability of the approach, formation and recognition, is investigated in detail by comparing fragment momentum space distributions {\it and simultaneously} their yields with recent experimental data of the $FOPI$ collaboration by varying the system size of the colliding system, i.e. its compressional energy (pressure, radial flow). We find an excellent agreement between theory and experiment in almost all the cases and, on the other hand, some limitations of the simple coalescence model. Furthermore, the temporal evolution of the fragment structure is explored with a clear evidence of an earlier formation of the heavier clusters, that will appear as interesting $relics$ of the high density phase of the nuclear Equation of State ($EoS$).Comment: 21 pages, 8 figures, Latex Elsart Style, minor corrections in p.7, two refs. added, Nucl.Phys.A, accepte

Self-consistent Approach to Off-Shell Transport

The properties of two forms of the gradient expanded Kadanoff--Baym equations, i.e. the Kadanoff--Baym and Botermans-Malfliet forms, suitable to describe the transport dynamics of particles and resonances with broad spectral widths, are discussed in context of conservation laws, the definition of a kinetic entropy and the possibility of numerical realization. Recent results on exact conservations of charge and energy-momentum within Kadanoff-Baym form of quantum kinetics based on local coupling schemes are extended to two cases relevant in many applications. These concern the interaction via a finite range potential, and, relevant in nuclear and hadron physics, e.g. for the pion--nucleon interaction, the case of derivative coupling.Comment: 35 pages, submitted to issue of Phys. Atom. Nucl. dedicated to S.T. Belyaev on the occasion of his 80th birthday. Few references are adde

Aspects of thermal and chemical equilibration of hadronic matter

We study thermal and chemical equilibration in 'infinite' hadron matter as well as in finite size relativistic nucleus-nucleus collisions using a BUU cascade transport model that contains resonance and string degrees-of-freedom. The 'infinite' hadron matter is simulated within a cubic box with periodic boundary conditions. The various equilibration times depend on baryon density and energy density and are much shorter for particles consisting of light quarks then for particles including strangeness. For kaons and antikaons the chemical equilibration time is found to be larger than $\simeq$ 40 fm/c for all baryon and energy densities considered. The inclusion of continuum excitations, i.e. hadron 'strings', leads to a limiting temperature of $T_s\simeq$ 150 MeV. We, furthermore, study the expansion of a hadronic fireball after equilibration. The slope parameters of the particles after expansion increase with their mass; the pions leave the fireball much faster then nucleons and accelerate subsequently heavier hadrons by rescattering ('pion wind'). If the system before expansion is close to the limiting temperature $T_s$, the slope parameters for all particles after expansion practically do not depend on (initial) energy and baryon density. Finally, the equilibration in relativistic nucleus-nucleus collision is considered. Since the reaction time here is much shorter than the equilibration time for strangeness, a chemical equilibrium of strange particles in heavy-ion collisions is not supported by our transport calculations. However, the various particle spectra can approximately be described within the blast model.Comment: 39 pages, LaTeX, including 18 postscript figures, Nucl. Phys. A, in pres