Towards a typology of intermediaries in sustainability transitions: a systematic review and a research agenda

Intermediary actors have been proposed as key catalysts that speed up change towards more sustainable socio-technical systems. Research on this topic has gradually gained traction since 2009, but has been complicated by the inconsistency regarding what intermediaries are in the context of such transitions and which activities they focus on, or should focus on. We briefly elaborate on the conceptual foundations of the studies of intermediaries in transitions, and how intermediaries have been connected to different transition theories. This shows the divergence – and sometimes a lack – of conceptual foundations in this research. In terms of transitions theories, many studies connect to the multi-level perspective and strategic niche management, while intermediaries in technological innovation systems and transition management have been much less explored. We aim to bring more clarity to the topic of intermediaries in transitions by providing a definition of transition intermediaries and a typology of five intermediary types that is sensitive to the emergence, neutrality and goals of intermediary actors as well as their context and level of action. Some intermediaries are specifically set up to facilitate transitions, while others grow into the role during the process of socio-technical change. Based on the study, as an important consideration for future innovation governance, we argue that systemic and niche intermediaries are the most crucial forms of intermediary actors in transitions, but they need to be complemented by a full ecology of intermediaries, including regime-based transition intermediaries, process intermediaries and user intermediaries

Commercialising university inventions for sustainability—a case study of (non-)intermediating ‘cleantech’ at Aalto University

The challenge to transform towards more sustainable societies requires action on multiple levels, including commercialisation of inventions created in universities. We examine intermediation in the pre-commercialisation phase of cleantech inventions developed at Aalto University, Finland, focusing on the activities of a university innovation intermediary, Aalto Centre for Entrepreneurship (ACE), and how it operationalises the sustainability aims of the university. The roles of ACE are discussed in the context of the university innovation ecosystem and through three cases of cleantech inventions. Surprisingly, we find that ACE does not include any ways to operationally integrate sustainability. The consideration of sustainability in commercialisation projects is case specific and fully dependent on other actors. As a result we propose people- and process-oriented alternatives of how sustainability could be integrated into university innovation support functions. We also propose that innovation ecosystems should be broadened to include public actors for the benefits of co-creating for sustainability

Introduction to ‘Markets in sustainability transitions’

If sustainability transitions research is to be relevant for upscaled diffusion of radical innovations and wide systemic socio-technical changes, then markets remain critical to account for. Founding frameworks in transition studies regard markets and market formation as important. Yet, the conceptualization of markets has so far not been elaborate: markets are mostly pictured as target areas for sustainable innovation and emphasis is on diffusion and user involvement. This special issue aims to unpack the notion of markets and market formation in sustainability transitions. The special issue contains in total 13 papers, which draw on theory from various scientific disciplines, use diverse research approaches, and cover a multitude of contexts. Altogether, the collection of papers stimulates broader theorizing of markets in sustainability transitions and the formulation of an agenda for future studies on markets in sustainability transitions. This editorial paper proposes relevant topics for such a research agenda

A feasibility study to investigate chemogenetic modulation of the locus coeruleus by means of single unit activity

Aim: Selective chemogenetic modulation of locus coeruleus (LC) neurons would allow dedicated investigation of the role of the LC-NA pathway in brain excitability and disorders such as epilepsy. This study investigated the feasibility of an experimental set-up where chemogenetic modification of the brainstem locus coeruleus NA neurons is aimed at and followed by LC unit activity recording in response to clozapine. Methods: The LC of male Sprague-Dawley rats was injected with 10 nl of adeno-associated viral vector AAV2/7-PRSx8-hM3Dq-mCherry (n = 19, DREADD group) or AAV2/7-PRSx8-eGFP (n = 13, Controls). Three weeks later, LC unit recordings were performed in anesthetized rats. We investigated whether clozapine, a drug known to bind to modified neurons expressing hM3Dq receptors, was able to increase the LC firing rate. Baseline unit activity was recorded followed by subsequent administration of 0.01 and 0.1 mg/kg of clozapine in all rats. hM3Dq-mcherry expression levels were investigated using immunofluorescence staining of brainstem slices at the end of the experiment. Results: Unit recordings could be performed in 12 rats and in a total of 12 neurons (DREADDs: n = 7, controls: n = 5). Clozapine 0.01 mg/kg did not affect the mean firing rate of recorded LC-neurons; 0.1 mg/kg induced an increased firing rate, irrespective whether neurons were recorded from DREADD or control rats (p = 0.006). Co-labeling of LC neurons and mCherry-tag showed that 20.6 +/- 2.3% LC neurons expressed the hM3Dq receptor. Aspecific expression of hM3Dq-mCherry was also observed in non-LC neurons (26.0 +/- 4.1%). Conclusion: LC unit recording is feasible in an experimental set-up following manipulations for DREADD induction. A relatively low transduction efficiency of the used AAV was found. In view of this finding, the effect of injected clozapine on LC-NA could not be investigated as a reliable outcome parameter for activation of chemogenetically modified LC neurons. The use of AAV2/7, a vector previously applied successfully to target dopaminergic neurons in the substantia nigra, leads to insufficient chemogenetic modification of the LC compared to transduction with AAV2/9

Pluralizing urban futures : A multicriteria mapping analysis of online taxis in Indonesia

The exploration of urban future storylines of transformative change is subject to socio-political processes rather than a mere, objective envisioning of the desirable city. Approaches in urban imagination and planning processes should thus consider plural perspectives across a range of actors and stakeholders beyond the usual suspects of experts and professionals.This paper mobilizes the case of the emergence of online taxis in Indonesia to embrace a more inclusive approach to the assessment of urban mobility futures by employing multi-criteria mapping (MCM) analysis and combining it with an open dialog on future storylines. We answer the question of what insights can be derived from diversifying future storylines in the online taxi industry in Indonesia? From applying a more inclusive approach in constructing future imaginaries we derive four insights: 1) criteria to appraise the future are never purely technological; 2) there is a difference in perceptions of time horizons among actors when imagining futures; 3) perceptions of time horizons are shaped by actor backgrounds and social interactions; and 4) the MCM method contributed to helping individuals to focus and explore their future storylines

Future of the drug label:Perspectives from a multistakeholder dialogue

'Regulating drugs does not end when market access has been granted. Monitoring drugs over the life-cycle has become state of the art, inherent to evolving legislation and societal need. Here, we explore how the drug label could move along in a changing playing-field, and become a sustainable label for the future. A dialogue between academia, government, the pharmaceutical industry, and patient/societal organizations was organized by the Regulatory Science Network Netherlands, RSNN. This is their view.

Replacing meat, an easy feat? The role of strategic categorizing in the rise of meat substitutes

This study investigates categories and categorization strategies in the transition towards sustainable diets. We look at the rise of meat substitutes and understand the meat substitute category as a hybrid one, covering products that are to replace meat while implicitly appealing to vegetarian/vegan diets. The category is negatively defined, solely and explicitly indicating the product to be substituted. The category's fuzzy nature presents an innovation challenge for producers. We investigate the strategies of nine Dutch meat substitute producers that seek to resemble meat along five dimensions. We conclude that by privileging meat resemblance innovation strategies, most producers tend to ‘fit and conform’ to the meat regime, largely copying dominant production and consumption practices. However, we also show that, as a hybrid category that is negatively defined, the meat substitute category provides opportunities for more radical innovations that have the potential to ‘stretch and transform’ the regime in the longer run

Servitisation on consumer markets: entry and strategy in Dutch private lease markets

Servitisation, which occurs when products are offered with service components as product-service bundles, has increased rapidly in consumer markets during recent years because of digitalisation. Digital technologies have enabled the emergence of peer-to-peer marketplaces and made it possible for B2B lease and rental actors to push for B2C markets. Despite extensive research on servitisation, we know little of what kinds of companies can best exploit the opportunities created by it and how digitalisation affects inter-company relationships regarding these opportunities. This article addresses these research gaps by making a revelatory case study on the entry order and strategy of established B2B lease companies to enter the B2C private leasing and carsharing markets. We collect an interview-based dataset on key companies in the Dutch car lease market, that we analyse abductively. We find that knowledge of the opportunities, position in the value chain, and resources are focal elements that define which companies are pioneers, early followers, and late entrants. In contrast to former servitisation literature, manufacturing incumbent companies are not active in exploiting opportunities created by private leasing. Additionally, we discover that the leasing companies create capabilities for private leasing themselves whereas they partner to enable carsharing. We discuss the contribution of these findings to research on disruptive innovation, servitisation, and digital innovation

Optimized parameters for transducing the locus coeruleus using canine adenovirus type 2 (CAV2) vector in rats for chemogenetic modulation research

Introduction The locus coeruleus noradrenergic (LC-NA) system is studied for its role in various neurological and psychiatric disorders such as epilepsy and Major Depression Dissorder. Chemogenetics is a powerful technique for specific manipulation of the LC to investigate its functioning. Local injection of AAV2/7 viral vectors has limitations with regards to efficiency and specificity of the transduction, potentially due to low tropism of AAV2/7 for LC neurons. In this study we used a canine adenovirus type 2 (CAV2) vector with different volumes and viral particle numbers to achieve high and selective expression of hM3Dq, an excitatory Designer Receptor Exclusively Activated by Designer Drugs (DREADD), for chemogenetic modulation of LC neurons. Methods Adult male Sprague-Dawley rats were injected in the LC with different absolute numbers of CAV2-PRSx8-hM3Dq-mCherry physical particles (0.1E9, 1E9, 5E9,10E9, or 20E9 pp) using different volumes (LowV = 3 nl x 300 nl, MediumV = 3 x 600 nl, HighV = 3 x 1200 nl). Two weeks post-injection, double-labeling immunohistochemistry for dopamine beta hydroxylase (DBH) and mCherry was performed to determine hM3Dq expression and its specificity for LC neurons. The size of the transduced LC was compared to the contralateral LC to identify signs of toxicity. Results Administration of Medium volume (3 x 600 nl) and 1E9 particles resulted in high expression levels with 87.3 +/- 9.8% of LC neurons expressing hM3Dq, but low specificity with 36.2 +/- 17.3% of hM3Dq expression in non-LC neurons. The most diluted conditions (Low volume_0.1E pp and Medium Volume_0.1E pp) presented similar high transduction of LC neurons (70.9 +/- 12.7 and 77.2 +/- 9.8%) with lower aspecificity (5.5 +/- 3.5 and 4.0 +/- 1.9%, respectively). Signs of toxicity were observed in all undiluted conditions as evidenced by a decreased size of the transduced LC. Conclusion This study identified optimal conditions (Low and Medium Volume with 0.1E9 particles of CAV2-PRSx8-hM3Dq-mCherry) for safe and specific transduction of LC neurons with excitatory DREADDs to study the role of the LC-NA system in health and disease