334 research outputs found

    Primary structure of tRNAval2 from brewer's yeast

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    Gas permeability in rarefied flow conditions for characterization of mineral membrane support

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    International audienceGas Permeability Measurement Technique (GPMT) has the advantage of being a non-destructive method, which is efficient in characterizing filtration membranes. Ceramic filtration membranes consist of successive layers of micro (support) to nano size (skin) pores. When gas flows through such a small scale structure, the molecular mean free path becomes comparable to the pore size. The Slip flow model, validated to describe the gas transport properties under rarefied flow conditions in a microchannel, is extended to porous media. The porous structure is modeled as a cluster of several identical cylindrical channels

    Optimization of the experimental set-up for a turbulent separated shear flow control by plasma actuator using genetic algorithms

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    Since 1947, when Schubauer and Skramstad established the basis of the technology with its revolutionary work about steady state tools and mechanisms for the flow management, the progress of the flow control technology and the development of devices have progressed constantly. Anyway, the applicability of such devices is limited, and only few of them have arrived to the assembly workshop. The problem is that the range of actuation is still limited. Despite their operability limitations, flow control devices are of great interest for the aeronautical industry. The number of projects investigating this technology demonstrates the relevance of in the Fluid Dynamic field. The scientific interest focus not only on the industrial applications and the improvement of the technology, but also on the deep understanding of the physical phenomena associated to the flow separation, turbulence formation associated to the final drag reduction aim. A clear example of what has been mentioned is the EC MARS research project (MARS project, FP7 project number 266326). Its objectives are aimed to a better understanding of the Reynolds Stress and turbulent flow related to both drag reduction and flow control. The research was carried out through the analysis of several flow control devices and the optimization of the parameters for some of them was an important element of the research. When solving a traditional fluid dynamics optimisation problem numerical flowanalysis are used instead of experimental ones due to their lower cost and shorter needed time for evaluation of candidate solutions. Nevertheless, in the particular case of the selected flow control plasma devices the experimental measurement of the performance of each candidate configuration has been much quicker than a numerical analysis. For this reason, the corresponding optimisation problem has been solved by coupling an evolutionary optimization algorithm with an experimental device. This paper discusses the design quality and efficiency gained by this innovative coupling.Peer ReviewedPostprint (author's final draft

    Multi-input genetic algorithm for experimental optimization of the reattachment downstream of a backward-facing step with surface plasma actuator

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    The practical interest of flow control approaches is no more debated as flow control provides an effective mean for considerably increasing the performances of ground or air transport systems, among many others applications. Here a fundamental configuration is investigated by using non-thermal surface plasma discharge. A dielectric barrier discharge is installed at the step corner of a backward-facing step (Reh=30000, Re¿=1650). Wall pressure sensors are used to estimate the reattaching location downstream of the step. The primary objective of this paper is the coupling of a numerical optimizer with an experiment. More specifically, optimization by genetic algorithm is implemented experimentally in order to minimize the reattachment point downstream of the step model. Validation through inverse problem is firstly demonstrated. When coupled with the plasma actuator and the wall pressure sensors, the genetic algorithm finds the optimum forcing conditions with a good convergence rate, the best control design variables being in agreement with the literature that uses other types of control devices than plasma. Indeed, the minimum reattaching position is achieved by forcing the flow at the shear layer mode where a large spreading rate is obtained by increasing the periodicity of the vortex street and by enhancing the vortex pairing phenomena. At the best forcing conditions, the mean flow reattachment is reduced by 20%. This article, with its experiment-based approach, demonstrates the robustness of a single-objective multi-design optimization method, and its feasibility for wind tunnel experiments.Postprint (published version

    ClbP is a prototype of a peptidase subgroup involved in biosynthesis of nonribosomal peptides

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    The pks genomic island of Escherichia coli encodes polyketide (PK) and nonribosomal peptide (NRP) synthases that allow assembly of a putative hybrid PK-NRP compound named colibactin that induces DNA double-strand breaks in eukaryotic cells. The pks-encoded machinery harbors an atypical essential protein, ClbP. ClbP crystal structure and mutagenesis experiments revealed a serine-active site and original structural features compatible with peptidase activity, which was detected by biochemical assays. Ten ClbP homologs were identified in silico in NRP genomic islands of closely and distantly related bacterial species. All tested ClbP homologs were able to complement a clbP-deficient E. coli mutant. ClbP is therefore a prototype of a new subfamily of extracytoplasmic peptidases probably involved in the maturation of NRP compounds. Such peptidases will be powerful tools for the manipulation of NRP biosynthetic pathways

    Quark propagator and vertex: systematic corrections of hypercubic artifacts from lattice simulations

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    This is the first part of a study of the quark propagator and the vertex function of the vector current on the lattice in the Landau gauge and using both Wilson-clover and overlap actions. In order to be able to identify lattice artifacts and to reach large momenta we use a range of lattice spacings. The lattice artifacts turn out to be exceedingly large in this study. We present a new and very efficient method to eliminate the hypercubic (anisotropy) artifacts based on a systematic expansion on hypercubic invariants which are not SO(4) invariant. A simpler version of this method has been used in previous works. This method is shown to be significantly more efficient than the popular ``democratic'' methods. It can of course be applied to the lattice simulations of many other physical quantities. The analysis indicates a hierarchy in the size of hypercubic artifacts: overlap larger than clover and propagator larger than vertex function. This pleads for the combined study of propagators and vertex functions via Ward identities.Comment: 14 pags., 9 fig
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