Faster N release, but not C loss, from leaf litter of invasives compared to native species in mediterranean ecosystems

Plant invasions can have relevant impacts on biogeochemical cycles, whose extent, in Mediterranean ecosystems, have not yet been systematically assessed comparing litter carbon (C) and nitrogen (N) dynamics between invasive plants and native communities. We carried out a 1-year litterbag experiment in 4 different plant communities (grassland, sand dune, riparian and mixed forests) on 8 invasives and 24 autochthonous plant species, used as control. Plant litter was characterized for mass loss, N release, proximate lignin and litter chemistry by 13C CPMAS NMR. Native and invasive species showed significant differences in litter chemical traits, with invaders generally showing higher N concentration and lower lignin/N ratio. Mass loss data revealed no consistent differences between native and invasive species, although some woody and vine invaders showed exceptionally high decomposition rate. In contrast, N release rate from litter was faster for invasive plants compared to native species. N concentration, lignin content and relative abundance of methoxyl and N-alkyl C region from 13C CPMAS NMR spectra were the parameters that better explained mass loss and N mineralization rates. Our findings demonstrate that during litter decomposition invasive species litter has no different decomposition rates but greater N release rate compared to natives. Accordingly, invasives are expected to affect N cycle in Mediterranean plant communities, possibly promoting a shift of plant assemblages

934 Artificial Liver Support in Children with Fulminant Hepatic Failure: Our Experience

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Another X-ray UFO without a momentum-boosted molecular outflow: ALMA CO(1–0) observations of the galaxy pair IRAS 05054+1718

We present Atacama Large Millimetre/submillimetre Array (ALMA) CO(1–0) observations of the nearby infrared luminous (LIRG) galaxy pair IRAS 05054+1718 (also known as CGCG 468-002), as well as a new analysis of X-ray data of this source collected between 2012 and 2021 using the Nuclear Spectroscopic Telescope Array (NuSTAR), Swift, and the XMM-Newton satellites. The western component of the pair, NED01, hosts a Seyfert 1.9 nucleus that is responsible for launching a powerful X-ray ultra-fast outflow (UFO). Our X-ray spectral analysis suggests that the UFO could be variable or multi-component in velocity, ranging from v/c ∼ −0.12 (as seen in Swift) to v/c ∼ −0.23 (as seen in NuSTAR), and constrains its momentum flux to be poutX−ray ∼ (4 ± 2) × 1034 g cm s−2. The ALMA CO(1–0) observations, obtained with an angular resolution of 2.200, although targeting mainly NED01, also include the eastern component of the pair, NED02, a less-studied LIRG with no clear evidence of an active galactic nucleus (AGN). We study the CO(1–0) kinematics in the two galaxies using the 3D-BAROLO code. In both sources we can model the bulk of the CO(1–0) emission with rotating disks and, after subtracting the best-fit models, we detect compact residual emission at S/N = 15 within ∼3 kpc of the centre. A molecular outflow in NED01, if present, cannot be brighter than such residuals, implying an upper limit on its outflow rate of M outmol . 19 ± 14 M yr−1 and on its momentum rate of pmolout . (2.7 ± 2.4) × 1034 g cm s−1. Combined with the revised energetics of the X-ray wind, we derive an upper limit on the momentum rate ratio of pmolout / pXout−ray < 0.67. We discuss these results in the context of the expectations of AGN feedback models, and we propose that the X-ray disk wind in NED01 has not significantly impacted the molecular gas reservoir (yet), and we can constrain its effect to be much smaller than expectations of AGN ‘energy-driven’ feedback models. We also consider and discuss the hypothesis of asymmetries of the molecular disk not properly captured by the 3D-BAROLO code. Our results highlight the challenges in testing the predictions of popular AGN disk-wind feedback theories, even in the presence of good-quality multi-wavelength observations

Spatial analysis of phylogenetic community structure: New version of a classical method

The increasing availability of phylogenetic information facilitates the use of evolutionary methods in community ecology to reveal the importance of evolution in the species assembly process. However, while several methods have been applied to a wide range of communities across different spatial scales with the purpose of detecting non-random phylogenetic patterns, the spatial aspects of phylogenetic community structure have received far less attention. Accordingly, the question for this study is: can point pattern analysis be used for revealing the phylogenetic structure of multi-species assemblages? We introduce a new individual-centered procedure for analyzing the scale-dependent phylogenetic structure of multi-species point patterns based on digitized field data. The method uses nested circular plots with increasing radii drawn around each individual plant and calculates the mean phylogenetic distance between the focal individual and all individuals located in the circular ring delimited by two successive radii. This scale-dependent value is then averaged over all individuals of the same species and the observed mean is compared to a null expectation with permutation procedures. The method detects particular radius values at which the point pattern of a single species exhibits maximum deviation from the expectation towards either phylogenetic aggregation or segregation. Its performance is illustrated using data from a grassland community in Hungary and simulated point patterns. The proposed method can be extended to virtually any distance function for species pairs, such as functional distances

Plastic leachate-induced toxicity during sea urchin embryonic development: Insights into the molecular pathways affected by PVC

This is the final version. Available on open access from Elsevier via the DOI in this recordData availability: Data will be made available on request.Microplastics are now polluting all seas and, while studies have found numerous negative interactions between plastic pollution and marine animals, the effects on embryonic development are poorly understood. A potentially important source of developmental ecotoxicity comes from chemicals leached from plastic particles to the marine environment. Here we investigate the effects of leachates from new and beach-collected pellets on the embryonic and larval development of the sea urchin Strongylocentrotus purpuratus and demonstrate that exposure of developing embryos to these leachates elicits severe, consistent and treatment-specific developmental abnormalities including radialisation of the embryo and malformation of the skeleton, neural and immune cells. Using a multi-omics approach we define the developmental pathways disturbed upon exposure to PVC leachates and provide a mechanistic view that pinpoints cellular redox stress and energy production as drivers of phenotypic abnormalities following exposure to PVC leachates. Analysis of leachates identified high concentrations of zinc that are the likely cause of these observed defects. Our findings point to clear and specific detrimental effects of marine plastic pollution on the development of echinoderms, demonstrating that chemicals leached from plastic particles into sea water can produce strong developmental abnormalities via specific pathways, and therefore have the potential to impact on a wide range of organisms.Human Frontiers Science ProgramEuropean Union Horizon 202

Transcriptomics and metabolomics integration reveals redox-dependent metabolic rewiring in breast cancer cells

Rewiring glucose metabolism toward aerobic glycolysis provides cancer cells with a rapid generation of pyruvate, ATP, and NADH, while pyruvate oxidation to lactate guarantees refueling of oxidized NAD+ to sustain glycolysis. CtPB2, an NADH-dependent transcriptional co-regulator, has been proposed to work as an NADH sensor, linking metabolism to epigenetic transcriptional reprogramming. By integrating metabolomics and transcriptomics in a triple-negative human breast cancer cell line, we show that genetic and pharmacological down-regulation of CtBP2 strongly reduces cell proliferation by modulating the redox balance, nucleotide synthesis, ROS generation, and scavenging. Our data highlight the critical role of NADH in controlling the oncogene-dependent crosstalk between metabolism and the epigenetically mediated transcriptional program that sustains energetic and anabolic demands in cancer cells

Effects of nitrogen seeding on core ion thermal transport in JET ILW L-mode plasmas

A set of experiments was carried out in JET ILW (Joint European Torus with ITER-Like Wall) L-mode plasmas in order to study the effects of light impurities on core ion thermal transport. N was puffed into some discharges and its profile was measured by active Charge Exchange diagnostics, while ICRH power was deposited on- and off-axis in (3 He) - D minority scheme in order to have a scan of local heat flux at constant total power with and without N injection. Experimentally, the ion temperature profiles are more peaked for similar heat fluxes when N is injected in the plasma. Gyro-kinetic simulations using the GENE code indicate that a stabilization of Ion Temperature Gradient driven turbulent transport due to main ion dilution and to changes in T_e /T_i and s / q is responsible of the enhanced peaking. The quasi-linear models TGLF and QuaLiKiz are tested against the experimental and the gyro-kinetic results.</p

Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development

How the vascular and neural compartment cooperate to achieve such a complex and highly specialized structure as the central nervous system is still unclear. Here, we reveal a crosstalk between motor neurons (MNs) and endothelial cells (ECs), necessary for the coordinated development of MNs. By analyzing cell-to-cell interaction profiles of the mouse developing spinal cord, we uncovered semaphorin 3C (Sema3C) and PlexinD1 as a communication axis between MNs and ECs. Using cell-specific knockout mice and in vitro assays, we demonstrate that removal of Sema3C in MNs, or its receptor PlexinD1 in ECs, results in premature and aberrant vascularization of MN columns. Those vascular defects impair MN axon exit from the spinal cord. Impaired PlexinD1 signaling in ECs also causes MN maturation defects at later stages. This study highlights the importance of a timely and spatially controlled communication between MNs and ECs for proper spinal cord development