The effects of sampling gear and environmental condition on the abundance estimates of freshwater zooplancton

L'abondance du zooplancton (Rotifères, Cladocères et Copépodes) a été évaluée à partir de 96 échantillons, la plupart provenant d'un petit réservoir eutrophique de Cordoba, Argentine. Trois types d'échantillonneurs ont été employés : deux filets (placés en avant et en arrière du canot, avec et sans brides), une pompe centrifuge submersible et une bouteille. Les résultats suggèrent que ni la taille du filet ni les brides n'ont un effet mesurable sur la récolte. De jour, les perturbations de la couche d'eau supérieure provoquent des réactions d'échappement diminuant ainsi l'efficacité des échantillonneurs, par contre les perturbations nocturnes ne produisent pas tel effet. Le zooplancton a été capturé plus efficacement avec les filets pendant la nuit ou par temps nuageux que par temps ensoleillé; ceci peut s'attribuer aux réactions optomotrices d'évitement. Par rapport aux échantillons de filets, la pompe et la bouteille sous-estiment la densité; la dispersion causée par perturbation est en partie responsable de cette différence. De plus, les trois groupes étudiés montrent de fortes réactions rhéotactiles en évitant activement l'aspiration de la pompe. Les différences d'efficacité atteignent parfois plusieurs ordres de grandeur. (Résumé d'auteur

Debate over the importance and meaning of native range in invasion biology: reply to Courchamp et al.

CommentDebate over the importance and meaning of nativerange in invasion biology: reply to Courchamp et al.Patricio Javier Pereyra1,2,3∗and Radu Cornel Guia¸su41Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos Almirante Storni (CIMAS), San Antonio Oeste,RioNegro,Argentina2Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina3Escuela Superior de Ciencias Marinas, Universidad Nacional del Comahue, San Antonio Oeste, Rio Negro, Argentina4Biology Program, Glendon College, York University, 2275 Bayview Avenue, Toronto, Ontario, M4N 3M6, CanadaIn their response to Pereyra (2020), Courchamp et al.(2020) considered problems with the concept of nativerange in invasion biology. They start by agreeing withPereyra that the concept of native range has limitations,but then proceed to criticize the work for what theyperceive as “flawed logical reasoning,” “a misleading se-lection of examples,” “cherry picking,” and a failure toappreciate the usefulness of this admittedly poorly de-fined concept. Here, we respond to the criticisms ofCourchamp et al., while addressing the important prob-lems that remain with the application of the native rangeconcept.Fil: Pereyra, Patricio Javier. Universidad Nacional del Comahue. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". - Provincia de Río Negro. Ministerio de Agricultura, Ganadería y Pesca. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni". Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Centro Nacional Patagónico. Centro de Investigación Aplicada y Transferencia Tecnológica en Recursos Marinos "Almirante Storni"; Argentina. Universidad Nacional del Comahue. Escuela de Ciencias Marinas; ArgentinaFil: Guiaşu, Radu Cornel. York University; Canad

Environmental controls on pteropod biogeography along the Western Antarctic Peninsula

Pteropods are abundant zooplankton in the Western Antarctic Peninsula (WAP) and important grazers of phytoplankton and prey for higher trophic levels. We analyzed long-term (1993-2017) trends in summer (January-February) abundance of WAP pteropods in relation to environmental controls (sea ice, sea surface temperature, climate indices, phytoplankton biomass and productivity, and carbonate chemistry) and interspecies dynamics using general linear models. There was no overall directional trend in abundance of thecosomes, Limacina helicina antarctica and Clio pyramidata, throughout the entire WAP, although L. antarctica abundance increased in the slope region and C. pyramidata abundance increased in the South. High L. antarctica abundance was strongly tied to a negative Multivariate El Nino Southern Oscillation Index the previous year. C. pyramidata abundance was best explained by early sea ice retreat 1-yr prior. Abundance of the gymnosome species, Clione antarctica and Spongiobranchaea australis, increased over the time series, particularly in the slope region. Gymnosome abundance was positively influenced by abundance of their prey, L. antarctica, during the same season, and late sea ice advance 2-yr prior. These trends indicate a shorter ice season promotes longer periods of open water in spring/summer favoring all pteropod species. Weak relationships were found between pteropod abundance and carbonate chemistry, and no long-term trend in carbonate parameters was detected. These factors indicate ocean acidification is not presently influencing WAP pteropod abundance. Pteropods are responsive to the considerable environmental variability on both temporal and spatial scales-key for predicting future effects of climate change on regional carbon cycling and plankton trophic interactions

Occurrence of toxigenic microalgal species and phycotoxins accumulation in mesozooplankton in Northern Patagonian gulfs, Argentina

In the Northern Patagonian gulfs of Argentina (Golfo Nuevo and Golfo San José), blooms of toxigenic microalgae and the detection of their associated phycotoxins are recurrent phenomena. The present study evaluated the transfer of phycotoxins from toxigenic microalgae to mesozooplankton in Golfo Nuevo and Golfo San José throughout an annual cycle (December 2014–2015 and January 2015–2016, respectively). In addition, solid‐phase adsorption toxin tracking (SPATT) samplers were deployed for the first time in these gulfs, to estimate the occurrence of phycotoxins in the seawater between the phytoplankton samplings. Domoic acid was present throughout the annual cycle in SPATT samplers, whereas no paralytic shellfish poisoning toxins were detected. Ten toxigenic species were identified: Alexandrium catenella, Dinophysis acuminata, Dinophysis acuta, Dinophysis tripos, Dinophysis caudata, Prorocentrum lima, Pseudo‐nitzschia australis, Pseudo‐nitzschia calliantha, Pseudo‐nitzschia fraudulenta, and Pseudo‐nitzschia pungens. Lipophilic and hydrophilic toxins were detected in phytoplankton and mesozooplankton from both gulfs. Pseudo‐nitzschia spp. were the toxigenic species most frequent in these gulfs. Consequently, domoic acid was the phycotoxin most abundantly detected and transferred to upper trophic levels. Spirolides were detected in phytoplankton and mesozooplankton for the first time in the study area. Likewise, dinophysistoxins were found in mesozooplankton from both gulfs, and this is the first report of the presence of these phycotoxins in zooplankton from the Argentine Sea. The dominance of calanoid copepods indicates that they were the primary vector of phycotoxins in the pelagic trophic web.Fil: D'Agostino, Valeria C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; ArgentinaFil: Krock, Bernd. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; AlemaniaFil: Degrati, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Sastre, Viviana. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Santinelli, Norma Herminia. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Krohn, Torben. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung; AlemaniaFil: Hoffmeyer, Mónica S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Tecnológica Nacional. Facultad Regional Bahía Blanca; Argentin

Molecular Detection of Invasive Species in Heterogeneous Mixtures Using a Microfluidic Carbon Nanotube Platform

Screening methods to prevent introductions of invasive species are critical for the protection of environmental and economic benefits provided by native species and uninvaded ecosystems. Coastal ecosystems worldwide remain vulnerable to damage from aquatic species introductions, particularly via ballast water discharge from ships. Because current ballast management practices are not completely effective, rapid and sensitive screening methods are needed for on-site testing of ships in transit. Here, we describe a detection technology based on a microfluidic chip containing DNA oligonucleotide functionalized carbon nanotubes. We demonstrate the efficacy of the chip using three ballast-transported species either established (Dreissena bugensis) or of potential threat (Eriocheir sinensis and Limnoperna fortuneii) to the Laurentian Great Lakes. With further refinement for on-board application, the technology could lead to real-time ballast water screening to improve ship-specific management and control decisions

Intra-Genomic Ribosomal RNA Polymorphism and Morphological Variation in Elphidium macellum Suggests Inter-Specific Hybridization in Foraminifera

Elphidium macellum is a benthic foraminifer commonly found in the Patagonian fjords. To test whether its highly variable morphotypes are ecophenotypes or different genotypes, we analysed 70 sequences of the SSU rRNA gene from 25 specimens. Unexpectedly, we identified 11 distinct ribotypes, with up to 5 ribotypes co-occurring within the same specimen. The ribotypes differ by varying blocks of sequence located at the end of stem-loop motifs in the three expansion segments specific to foraminifera. These changes, distinct from typical SNPs and indels, directly affect the structure of the expansion segments. Their mosaic distribution suggests that ribotypes originated by recombination of two or more clusters of ribosomal genes. We propose that this expansion segment polymorphism (ESP) could originate from hybridization of morphologically different populations of Patagonian Elphidium. We speculate that the complex geological history of Patagonia enhanced divergence of coastal foraminiferal species and contributed to increasing genetic and morphological variation