Plant Growth Promoting Properties of Four Arctic Seaweed Extracts

The objective of this study was to evaluate the effects of the White Sea brown seaweed Ascophyllum nodosum, Fucus vesiculosus, Laminaria digitata and Saccharina latissimi extracts on plant growth and biomass accumulation. Extracts were obtained by isopropanol maceration method. Growth promoting effects of all four seaweed extracts were observed at the initial growth phase of Triticum aestivum and Cucumis sativus plants. A. nodosum and F. vesiculosus extracts were more effective at medium concentrations (60, 120 and 300 mg/L) while higher concentration (1200 mg/L) decreased the growth rate and biomass accumulation in wheat plants, but not in cucumber seedlings when compared to control. Higher phenolic content in A. nodosum and F. vesiculosus extracts can possibly be a reason of lower effectiveness of extracts with high concentration. These results suggest that investigated arctic seaweeds have biostimulatory properties that affect plant growth and biomass accumulation and after intensive future studies can be exploited for elaboration of innovative products for agriculture

A validated ultra-performance liquid chromatography with diode array detection coupled to electrospray ionization and triple quadrupole mass spectrometry method to simultaneously quantify taurine, homotaurine, hypotaurine and amino acids in macro- and microalgae

A fast and reliable method for the simultaneous quantification of Taurine, Homotaurine, Hypotaurine and 19 amino acids in algae samples by Ultra-performance liquid chromatography coupled with diode array and tandem mass spectrometry (UHPLC–DAD-MS/MS) was optimized and validated. Target compounds were chromatographically resolved in less than 15 min. (ESI)-MS/MS electrospray ionization and pure analytical standards were used to confirm the identity of all analytes, while quantitation was carried out with diode array detection. Validation parameters of the method were satisfactory: Resolution of peak pairs was always higher than 1.55; all analytical curves showed R2 > 0.99, with working ranges between 0.04 mg/g to 33.1 mg/g and 9.13 mg/g to 107 mg/g and the Lack-of-fit test was not significant. The intra and inter-day precision of the method (expressed as relative standard deviation) were lower than 6% and recovery values ranged between 95% and 105%. The method was demonstrated to be robust to small deliberate variations of seven variables such sample weight, volume of hydrolysis reagent, hydrolysis time and temperature, derivatization time, column temperature and flow rate. The mean expanded uncertainty for all the target compounds were 0.7 mg/g with a coverage factor of 2. Method Limits of detection and quantification varied from 0.005 * 10−3 mg/g to 0.11 * 10−3 mg/g and 0.01* 10−3 mg/g to 0.22 * 10-3 mg/g respectively, allowing the routine determination of these bioactive compounds in algae extracts. Therefore, the method was successfully applied for the quantitative determination of the 22 target compounds in five seaweed commercial samples. Relevant compounds were quantified for the first time in the five algae species, namely: i) Taurine in Gracilaria longissima and Chlorella spp., ii) Gamma-aminobutyric acid in G. longissima and L. japonica, iii) Hydroxyproline in G. longissima, Ulva lactuca, Porphyra spp., and L. japonica and iv) Homotaurine and Hypotaurine in the five species studied.info:eu-repo/semantics/acceptedVersio

МЕХАНИЗМ СТАБИЛЬНОСТИ ТОРФЯНЫХ БИТУМОВ

It has been established that the reason for stability of peat bitumes during metamorphism process is the presence of “quasipolyconjugation” systems caused by synergistic effect of the interaction of individual aromatic rings and hydrogen bondings functional groups.Установлено, что стабильность торфяных битумов в процессе метаморфизма обязана наличию систем «квазипо­ лисопряжения», обусловленных синергическим эффектом взаимодействия одиночных ароматических колец и водо­ родных связей функциональных групп

Integral indices for quality assessment of pulp and paper mill effluents

The first part of this investigation studied complex nature of chemical oxygen demand (COD ) parameter on local and overall effluents, which were obtained from two pulp and paper mills, located in the Northwest region of Russia. Contribution of individual compounds and fractions of matters having different chemical nature to COD for these effluents was established. The second part of this study shows that, qualitative and quantitative characteristics of priority pollutants and their contribution to COD for different pulp and paper mills are not the same, though these mills use similar technologies and produce similar products

Plant Growth Promoting Properties of Four Arctic Seaweed Extracts

The objective of this study was to evaluate the effects of the White Sea brown seaweed Ascophyllum nodosum, Fucus vesiculosus, Laminaria digitata and Saccharina latissimi extracts on plant growth and biomass accumulation. Extracts were obtained by isopropanol maceration method. Growth promoting effects of all four seaweed extracts were observed at the initial growth phase of Triticum aestivum and Cucumis sativus plants. A. nodosum and F. vesiculosus extracts were more effective at medium concentrations (60, 120 and 300 mg/L) while higher concentration (1200 mg/L) decreased the growth rate and biomass accumulation in wheat plants, but not in cucumber seedlings when compared to control. Higher phenolic content in A. nodosum and F. vesiculosus extracts can possibly be a reason of lower effectiveness of extracts with high concentration. These results suggest that investigated arctic seaweeds have biostimulatory properties that affect plant growth and biomass accumulation and after intensive future studies can be exploited for elaboration of innovative products for agriculture

Componential composition and antibacterial activity of essential oil of wood greenery of Juniperus communis L. in the Subarctic region of Russia

Common juniper Juniperus communis L. is one of the most valuable raw material sources of essential oils in the Subarctic region of Russia. It is obligatory dioecious species, found in the underbrush in almost all forest types on the northern border of its range. The chemical composition of essential oils obtained from wood greenery of male and female trees of common juniper, growing in the natural biocoenoses of the northern taiga subzone is presented in the article. The results showed variations in the yields of juniper essential oils depending on the gender of trees (3.95 % (v/w dried materials) from male trees and 4.65 % from female trees in the initial growing season). Twenty eight terpene compounds with content of more than 0.1 % were identified by chromatography-mass spectrometry. The most representative fraction of the essential oil of male and female juniper trees is monoterpene hydrocarbons, 67.98 % (of the total essential oils) in male trees and 60.95 % in female trees. The essential oil of male trees is characterized by the highest total yield of mono- and sesquiterpene hydrocarbons, whereas the highest content of oxygen-containing derivatives of terpenes is noted for the essential oil of female trees. According to the quantitative composition data, the Common juniper essential oils contain a wide range of biologically active substances that exhibit a variety of pharmacological properties. The antibacterial activity of the essential oils of both female and male trees junipers was evaluated against 3 bacteria: colibacillus Escherichia coli Migula ATCC 25922; goldish staphylococcus Staphylococcus aureus Rosenbach ATCC 6538; blue pus organism Pseudomonas aeruginosa (Schroeter) Migula ATCC 27853, by the paper disk diffusion method. The essential oils of Common juniper showed a broad spectrum of antibacterial activity against gram-negative bacteria. The results demonstrated the perspective of using wood greenery juniper like a pharmaceutical substance for obtaining essential oils as components of plant compounds and natural products with antibacterial properties