Union with Christ in the Writings of Ellen G. White

Building on the work of nineteenth-century theologian Augustus Strong, five historical approaches to union with Christ identified by Bruce Demarest in the late twentieth century include: an ontological union, a sacramental union, a covenantal union, a moral or filial union and an experiential union. Given the identification of multiple approaches to union with Christ, my dissertation attempts to clarify Ellen G. White’s concept of union with Christ using Demarest’s categories as an evaluation tool. I traced the development of her writings on union with Christ from 1860 to 1898 to ask whether White’s approach is best described as an ontological, sacramental, covenantal, moral or filial or experiential union. While elements of some of the approaches Demarest identified are evident in White’s writings, such as the need for believers to experience Christ for themselves, no one category fits White’s approach exclusively. White’s approach can best be described using her own language. Union with Christ is a spiritual, vital, heart, mystical, indissoluble, moral union that connects repentant sinners to Christ and other believers. Her key metaphors used to describe the nature of this relationship are the vine and the branches, the mystic ladder and the golden chain. The vine and the branches explain how believers are intimately connected to Christ, receiving moral and spiritual power through the Holy Spirit who makes Christ present within. The mystic ladder illustrates Christ’s incarnation that makes union with Him possible. White also used the mystic ladder to describe the progressive nature of sanctification, ascending the ladder by faith and perseverance. The golden chain illustrates the love of the Father poured into the hearts of believers. For White, Christ metaphorically is the true vine, the mystic ladder and the golden chain. Believers partake of the divine nature and by exercising faith in Christ, become one with God. In my conclusion, I answered the following additional questions in relation to White’s writings: What is union with Christ? What is the nature of union with Christ? How is it formed? How is it maintained? What are its consequences? In brief, union with Christ is a believer’s free will, spiritual relationship with Christ. It is formed by repentance and faith and is maintained by living, active faith. Engaging in spiritual disciplines, may strengthen a believer’s union with Christ through prayer and Scripture reading, the daily surrender of the will, engaging with others and faith in Christ. Its consequences are multiple resulting in a transformed life and character. They include justification, sanctification, obedience and participation in the mission of Christ to redeem humanity. Christ’s goal is to reunite the inhabitants of heaven and earth by an indissoluble tie through a vital, spiritual relationship with Himself and the Godhead

Water-soluble cranberry extract inhibits \u3ci\u3eVibrio cholerae\u3c/i\u3e biofilm formation possibly through modulating the second messenger 3’, 5’ - Cyclic diguanylate level

Quorum sensing (QS) and nucleotide-based second messengers are vital signaling systems that regulate bacterial physiology in response to changing environments. Disrupting bacterial signal transduction is a promising direction to combat infectious diseases, and QS and the second messengers are undoubtedly potential targets. In Vibrio cholerae, both QS and the second messenger 3’, 5’—cyclic diguanylate (c-di-GMP) play a central role in controlling motility, motile-to-sessile life transition, and virulence. In this study, we found that water-soluble extract from the North American cranberry could significantly inhibit V. cholerae biofilm formation during the development/maturation stage by reducing the biofilm matrix production and secretion. The anti-biofilm effect by water-soluble cranberry extract was possibly through modulating the intracellular c-di-GMP level and was independent of QS and the QS master regulator HapR. Our results suggest an opportunity to explore more functional foods to fight stubborn infections through interference with the bacterial signaling systems

Fast simulation of crowd collision avoidance

Real-time large-scale crowd simulations with realistic behavior, are important for many application areas. On CPUs, the ORCA pedestrian steering model is often used for agent-based pedestrian simulations. This paper introduces a technique for running the ORCA pedestrian steering model on the GPU. Performance improvements of up to 30 times greater than a multi-core CPU model are demonstrated. This improvement is achieved through a specialized linear program solver on the GPU and spatial partitioning of information sharing. This allows over 100,000 people to be simulated in real time (60 frames per second)

Strichartz estimates on Schwarzschild black hole backgrounds

We study dispersive properties for the wave equation in the Schwarzschild space-time. The first result we obtain is a local energy estimate. This is then used, following the spirit of earlier work of Metcalfe-Tataru, in order to establish global-in-time Strichartz estimates. A considerable part of the paper is devoted to a precise analysis of solutions near the trapping region, namely the photon sphere.Comment: 44 pages; typos fixed, minor modifications in several place

A single-nucleus RNA-sequencing pipeline to decipher the molecular anatomy and pathophysiology of human kidneys

Defining cellular and molecular identities within the kidney is necessary to understand its organization and function in health and disease. Here we demonstrate a reproducible method with minimal artifacts for single-nucleus Droplet-based RNA sequencing (snDrop-Seq) that we use to resolve thirty distinct cell populations in human adult kidney. We define molecular transition states along more than ten nephron segments spanning two major kidney regions. We further delineate cell type-specific expression of genes associated with chronic kidney disease, diabetes and hypertension, providing insight into possible targeted therapies. This includes expression of a hypertension-associated mechano-sensory ion channel in mesangial cells, and identification of proximal tubule cell populations defined by pathogenic expression signatures. Our fully optimized, quality-controlled transcriptomic profiling pipeline constitutes a tool for the generation of healthy and diseased molecular atlases applicable to clinical samples

Platform for enhanced detection efficiency in luminescence-based sensors

Luminescence-based biochip measurement platforms are employed in a wide range of biological applications, such as biomedical diagnostics. Based on an understanding of the anisotropic emission properties of luminescence emitters close to a dielectric interface, a simple strategy for producing a better than 25-fold enhancement of the detected luminescence is presented. This strategy is demonstrated for low cost polymer platforms compatible with mass-production

Genetic approach to track neural cell fate decisions using human embryonic stem cells

With their capability to undergo unlimited self-renewal and to differentiate into all cell types in the body, human embryonic stem cells (hESCs) hold great promise in human cell therapy. However, there are limited tools for easily identifying and isolating live hESC-derived cells. To track hESC-derived neural progenitor cells (NPCs), we applied homologous recombination to knock-in the mCherry gene into the Nestin locus of hESCs. This facilitated the genetic labeling of Nestin positive neural progenitor cells with mCherry. Our reporter system enables the visualization of neural induction from hESCs both in vitro (embryoid bodies) and in vivo (teratomas). This system also permits the identification of different neural subpopulations based on the intensity of our fluorescent reporter. In this context, a high level of mCherry expression showed enrichment for neural progenitors, while lower mCherry corresponded with more committed neural states. Combination of mCherry high expression with cell surface antigen staining enabled further enrichment of hESC-derived NPCs. These mCherry(+) NPCs could be expanded in culture and their differentiation resulted in a down-regulation of mCherry consistent with the loss of Nestin expression. Therefore, we have developed a fluorescent reporter system that can be used to trace neural differentiation events of hESCs

On the massive wave equation on slowly rotating Kerr-AdS spacetimes

The massive wave equation $\Box_g \psi - \alpha\frac{\Lambda}{3} \psi = 0$ is studied on a fixed Kerr-anti de Sitter background $(\mathcal{M},g_{M,a,\Lambda})$. We first prove that in the Schwarzschild case (a=0), $\psi$ remains uniformly bounded on the black hole exterior provided that $\alpha < {9/4}$, i.e. the Breitenlohner-Freedman bound holds. Our proof is based on vectorfield multipliers and commutators: The usual energy current arising from the timelike Killing vector field $T$ (which fails to be non-negative pointwise) is shown to be non-negative with the help of a Hardy inequality after integration over a spacelike slice. In addition to $T$, we construct a vectorfield whose energy identity captures the redshift producing good estimates close to the horizon. The argument is finally generalized to slowly rotating Kerr-AdS backgrounds. This is achieved by replacing the Killing vectorfield $T=\partial_t$ with $K=\partial_t + \lambda \partial_\phi$ for an appropriate $\lambda \sim a$, which is also Killing and--in contrast to the asymptotically flat case--everywhere causal on the black hole exterior. The separability properties of the wave equation on Kerr-AdS are not used. As a consequence, the theorem also applies to spacetimes sufficiently close to the Kerr-AdS spacetime, as long as they admit a causal Killing field $K$ which is null on the horizon.Comment: 1 figure; typos corrected, references added, introduction revised; to appear in CM