Microbiological and health related perspectives of geophagia: An overview

Geophagia, which refers to the deliberate eating of soil, is considered to be a deviant eating disorder, a sequel to poverty and famine but could also be observed in the absence of hunger and in both scenarios may be associated with high degree of mortality and morbidity. The phenomenon has been reported to be common among pregnant women, lactating women, school children and people with psychiatric disorders. The microbiology of soil shows a broad diversity and functionality of soil microflora which impact variously on soil and its consumption. Soil microbes contribute immensely to the quality of soil and even determine soil types. Geophagia may be beneficial or harmful. Beneficial aspects include the use of kaolin to treat diarrhea, gastritis, colitis, enhancement of bioactivities and maintenance of normal intestinal flora by commensal flora found in soil. Clay or soil containing special constituents are valuable oral and topical antimicrobials as well as adsorbents of toxins. Microbiological underpinnings of geophagia include the ingestion of eggs of parasitic worms such as Ascaris lumbricoides, Trichuris trichiura with the health consequences. Highly toxigenic bacteria such as Clostridium perfringens, Clostridium tetani, Clostridium botulinum, the causative agents of gas gangrene, tetanus and botulism and other human pathogens may be ingested. It has also been postulated that indirect consumption of soil may pose serious health problems. For example, nitrate run-off and leaching from soil into water bodies may lead to eutrophication and colonization by toxic cyanobacteria with ripple medical effects such as gastroenteritis. Some general health implications of geophagia include association with iron deficiency and anemia, intestinal obstruction, constipation, peritonitis, dental damage, eclampsia, iron deficiency and even mortality. The interplay of factors involved in geophagia, though varied, intricate and researched may not have been fully elucidated. Further concerted efforts aimed at multidisciplinary research are warranted so as to address gaps in the corpus of knowledge on the important subject.Keywords: Geohelminths, geophagia, health, microorganisms, soilAfrican Journal of Biotechnology Vol. 9(19), pp. 5784-5791, 10 May, 201

A survey of indigenous herbal diarrhoeal remedies of O.R. Tambo district, Eastern Cape Province, South Africa

Indigenous health system and the use of herbal plants have been recognized as pivotal in primary health care and a system to reckon with in achieving one of the targets of the millennial goals on health.An ethnobotanical survey was conducted to identify indigenous herbal remedies for diarrhoea and associated stomach ailments in rural areas of the O.R. Tambo district municipality in the Eastern Cape province of South Africa. The main objective of the study was to gather ethnomedical data onpotentially valuable indigenous medicinal plants for the eventual development of new pharmaceuticals and also emphasize the role of ethnomedicine in primary health care. The use of herbal remedies in thetreatment of diarrhoea and dysentery was investigated using interviews. The survey was conducted among traditional healers and knowledgeable local elders who use medicinal plants to treat common illnesses. Data from the survey indicated the names of plants commonly used in the treatment of diarrhoea and associated ailments, the methods of preparation, parts used and administration. A total of 32 plant species belonging to 26 families were reportedly used as diarrhoea remedy in the study area. The most predominant families of medicinal plants employed and most frequently recommended were Fabaceae (16.67%), followed by Hyacinthaceae and Hydnoraceae (8.33% each). The most commonly utilized portions of plants for medicinal purposes included roots and leaves. Other parts were corms, bulbs, tubers, fruits and bark. The methods of preparation often employed were decoctions and infusions whilst medication was frequently administered orally or as enema. Some of the plants were used singly or mixed with other plant(s) while some edible ones are consumed as food. The survey documented a diversity of plants employed as remedy for diarrhoea. Integration of this form of health care system into western medicine is warranted. The propagation of such medicinal plants is vital for sustainable use of these medicinal plants

Molecular characterization, antibiograms and antibacterial activities of selected medicinal plants against some enteric pathogens

Diarrhea diseases remain one of the greatest health problems in many parts of the world. In some cases, the disease is mild and self-limiting; however, the symptoms may be very severe in the elderly and young children (Smith and Cheasty, 1998), as well as in immune compromised patients such as HIV/AIDS patients. The disease is one of the hallmarks of HIV/AIDS in developing countries and is also a cardinal clinical manifestation of water borne infections (Obi et al., 2007). According to a World Health Organization report (WHO, 2004), diarrhea was rated second, after respiratory infections out of the eight deadliest diseases worldwide and was responsible for 1.8 million deaths per year. This infectious disease which can cause dehydration is primarily a symptom of gastrointestinal infection, but chemical irritation of the gut or non-infectious bowel disease can also result in diarrhoe. Studies have shown that the predominant causative agents of acute and persistent diarrhoe are Gram-negative rods such as Escherichia coli, Vibrio cholerae, Aeromonas, Campylobacter, Salmonella species, Shigella species, Plesiomonas shigelloides and Yersinia enterocolitica (Obi et al., 1995, 1998, 2003; Lainson and Silva, 1999; Coker et al., 2002; Oyofo et al., 2002). Aside from bacterial agents of diarrhoea, other causative pathogens include protozoa such as Giardia lamblia and Cryptosporidium parvum; viruses such as norwalk virus and rota virus. Although fungal agents such as Candida have been shown to be prevalent in children with diarrhoe (Enweani et al. 1994), a more recent study (Forbes et al., 2001), associated higher Candida counts with recent antibiotic use. Faecal concentrations of Candida were higher in patients with diarrhoe, but the study confirmed no association between faecal candida or other yeasts and diarrhoe. Norwalk viruses, calci-like viruses and rota viruses are the major viral agents of diarrhoe. According to Parashar et al., (2006), rota virus is the leading cause of diarrhoe hospitalisation among children worldwide, causing 440,000 annual deaths in children under 5 years of age

Molecular basis of virulence in clinical isolates of Escherichia coli and Salmonella species from a tertiary hospital in the Eastern Cape, South Africa

<p>Abstract</p> <p>Background</p> <p>Apart from localized gastrointestinal infections, <it>Escherichia coli </it>and <it>Salmonella </it>species are major causes of systemic disease in both humans and animals. <it>Salmonella </it>spp. cause invasive infections such as enteric fever, septicemia, osteomyelitis and meningitis while certain types of <it>E. coli </it>can cause systemic infections, including</p> <p>pyelonephritis, meningitis and septicemia. These characteristic requires the involvement of a myriad of virulence factors.</p> <p>Methods</p> <p>This study investigated the virulence factors of <it>Escherichia coli </it>and <it>Salmonella </it>species in clinical specimens from patients with diarrhoea presenting to health care centres in Oliver R. Tambo District Municipality, Eastern Cape Province, Republic of South Africa. Microbiology analysis involved the use of cultural and molecular techniques.</p> <p>Results</p> <p>Out of a total of 315 samples screened, <it>Salmonella </it>isolates were obtained in 119 (37.8%) of cases and these comprised: <it>S. choleraesuis </it>(6%), <it>S. enteritidis </it>(4%), <it>S. eppendorf </it>(1%), <it>S. hadar </it>(1%), <it>S. isangi </it>(8%), <it>S. panama </it>(1%), <it>S. typhi </it>(52%), <it>S. typhimurium </it>(25%) and untyped <it>Salmonella </it>spp. (2%). Among the <it>Salmonella </it>species 87 (73.1%) were invasive. Using molecular diagnostic methods, diarrheagenic <it>E. coli </it>were detected in 90 cases (28.6%): the greater proportion of this were enteroaggregative <it>E. coli </it>(EAEC) 37 (41.1%), enteropathogenic <it>E. coli </it>(EPEC) 21 (23.3%) and enterohemorrhagic <it>E. coli </it>(EHEC) 21 (23.3%). The predominant virulence gene among the diarrheagenic <it>E. coli </it>was EAEC heat-stable enterotoxin <it>astA </it>genes while the virulence genes identified in the <it>Salmonella </it>strains were 15 (12.6%) flic and 105 (88.2%) inv genes. The amino acid identity of the representative genes showed 95-100% similarity to corresponding blast searched sequence.</p> <p>Conclusions</p> <p>This study showed the diversity of virulence gene expression in two major enteric pathogens. <it>S. typhi </it>and enteroaggregative <it>E. coli </it>were the predominant enteropathogens in our study area with an indication that EAEC is endemic within our study population. It was observed among other things that some diarrheagenic <it>E. coli </it>isolated from apparently asymptomatic subjects expressed some virulence genes at frequency as high as seen in diarrheagenic cases. This study underlines the importance of understanding the virulence composition and diversity of pathogens for enhanced clinico-epidemiological monitoring and health care delivery.</p

Microbial Evaluation of Geophagic and Cosmetic Clays from Southern and Western Nigeria: Potential Natural Nanomaterials

Geophagic and cosmetic clays are among potential nano-material which occur naturally and are of various forms. The use of these nano-clays is a common practice in both rural and urban areas mostly due to tradition and medicinal reasons. These naturally occurring materials can be valuable sources of nano-material by serving as nano-composites. The need to ascertain the safety of these materials is the motivation for this research. Physical Characterization based on the hue value and microbiological qualities of the nano-clays were carried out. The Microbial analysis of the clay samples showed considerable contamination with both bacteria and fungi with fungal contaminants taking the lead. This observation may not be unlikely due to the ability of fungi species to survive harsher growth conditions than bacteria. 'Atike pupa' showed no bacterial growth. The clay with the largest bacterial count was Calabash chalk (Igbanke), while that with the highest fungal count was 'Eko grey'. The most commonly isolated bacteria in this study were Clostridium spp. and Corynebacterium spp. while fungi included Aspergillus spp. These results are an indication of the need to subject these clay materials to treatments such as heating before consumption or topical usage thereby ascertaining their safety

Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea

Abstract Background This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa. Methods The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch.) van der Merwe were screened against Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, multi-resistant Salmonella enterica serovar Isangi, S. typhi, S. enterica serovar Typhimurium, Shigella flexneri type 1b and Sh. sonnei phase II. A qualitative phytochemical screening of the plants extracts was by thin layer chromatography. Plants extracts were screened for antibacterial activity using serial dilution microplate technique and bioautography. Results The TLC fingerprint indicated the presence of terpenoids and flavonoids in the herbs. Most of the tested organisms were sensitive to the crude acetone extracts with minimum inhibitory concentration (MIC) values ranging from 0.018–2.5 mg/mℓ. Extracts of A. striata, C. uncinulata, E. autumnalis and P. guajava were more active against enteropathogens. S. aureus and Sh. flexneri were the most sensitive isolates to the crude extracts but of significance is the antibacterial activity of A. arborescens and P. guajava against a confirmed extended spectrum betalactamase positive S. enterica serovar Typhimurium. Conclusion The presence of bioactive compounds and the antibacterial activity of some of the selected herbs against multidrug resistant enteric agents corroborate assertions by traditional healers on their efficacies

Evaluation of the antibacterial and anticancer activities of some South African medicinal plants

<p>Abstract</p> <p>Background</p> <p>Several herbs are traditionally used in the treatment of a variety of ailments particularly in the rural areas of South Africa where herbal medicine is mainly the source of health care system. Many of these herbs have not been assessed for safety or toxicity to tissue or organs of the mammalian recipients.</p> <p>Methods</p> <p>This study evaluated the cytotoxicity of some medicinal plants used, inter alia, in the treatment of diarrhoea, and stomach disorders. Six selected medicinal plants were assessed for their antibacterial activities against ampicillin-resistant and kanamycin-resistant strains of <it>Escherichia coli </it>by the broth micro-dilution methods. The cytotoxicities of methanol extracts and fractions of the six selected plants were determined using a modified tetrazolium-based colorimetric assay (3-(4, 5-dimethylthiazol)-2, 5-diphenyl tetrazolium bromide (MTT) assay).</p> <p>Results</p> <p>The average minimum inhibitory concentration (MIC) values of the plants extracts ranged from 0.027 mg/mℓ to 2.5 mg/mℓ after 24 h of incubation. <it>Eucomis autumnalis </it>and <it>Cyathula uncinulata </it>had the most significant biological activity with the least MIC values. The in vitro cytotoxicity assay on human hepatocarcinoma cell line (Huh-7) revealed that the methanol extract of <it>E. autumnalis </it>had the strongest cytotoxicity with IC₅₀of 7.8 μg/mℓ. Ethyl acetate and butanol fractions of <it>C. uncinulata, Hypoxis latifolia, E. autumnalis </it>and <it>Lantana camara </it>had lower cytotoxic effects on the cancer cell lines tested with IC₅₀values ranging from 24.8 to 44.1 μg/mℓ; while all the fractions of <it>Aloe arborescens </it>and <it>A. striatula </it>had insignificant or no cytotoxic effects after 72 h of treatment.</p> <p>Conclusions</p> <p>Our results indicate that the methanol fraction of <it>E. autumnalis </it>had a profound cytotoxic effect even though it possessed very significant antibacterial activity. This puts a query on its safety and hence a call for caution in its usage, thus a product being natural is not tantamount to being entirely safe. However, the antibacterial activities and non-cytotoxic effects of <it>A. arborescens </it>and <it>A. striatula </it>validates their continuous usage in ethnomedicine.</p

Interactive efficacies of Elephantorrhiza elephantina and Pentanisia prunelloides extracts and isolated compounds against gastrointestinal bacteria

Elephantorrhiza elephantina (Burch.) Skeels (Fabaceae) and Pentanisia prunelloides (Klotzsch ex Eckl. & Zeyh.) Walp. (Rubiaceae) are two medicinal plants used extensively in southern Africa to treat various ailments. Often, decoctions and infusions from these two plants are used in combination specifically for stomach ailments. The antimicrobial activities of the methanol and aqueous extracts of the rhizomes of the two plants, as well as the two active ingredients from the plants [(−)-epicatechin and palmitic acid] have been determined apart and in combination against Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 8739) and Bacillus cereus (ATCC 11778). The minimum inhibitory concentration (MIC) values for the aqueous (0.50–16.00 mg/mL) and methanol (0.20–16.00 mg/mL) extracts independently demonstrated varied efficacies depending on the pathogen of study. When the two plants were combined in 1:1 ratios, synergistic to additive interactions (ΣFIC values 0.19–1.00) were noted. Efficacy for the two major compounds ranged between 0.13–0.63 mg/mL and mainly synergistic interactions were noted against E. faecalis and E. coli. The predominantly synergistic interactions noted between E. elephantina and P. prunelloides and major compounds, when tested in various ratios against these pathogens, provide some validation as to the traditional use of these two plants to treat bacterial gastrointestinal infections