Investigation of Microbiota in Health and Disease of Poultry

The microbiotas play vital roles in health and diseases of both humans and animals. 16S rRNA genes sequence analysis is one of the most popular and commonly used methods in the analysis of microbiotas associated with hosts. In this dissertation, the microbiotas of chickens (broilers, breeders, and layers) and turkeys were evaluated by 16S rRNA gene sequencing. Characterization of the culturable subpopulations of Lactobacillus in the chicken gut can serve as a valuable resource for probiotic development. In Chapter 2, Lactobacillus subpopulations recovered on MRS from chicken gut were defined comprehensively for the first time using 16S rRNA gene profiling, where they varied with different regions (cecum vs. ileum) and locations (lumen vs. mucosa) with in the same region. In Chapter 3, we investigated the effect of cell densities as determined by varying levels of sample dilution on the culture-enriched microbiota profiles using MRS agar medium as a model system. The dilution levels of original samples was found to alter the resulting culture-enriched microbiota profiles via unknown density-dependent mechanisms. In chapter 4, Bacillus isolates (B. subtilis and B. amyloliquefaciens) were used to evaluate their therapeutic and prophylactic effects against Salmonella Enteritidis, and found their potentialities to reduce S. Enteritidis colonization and improve the intestinal health in broiler chickens possibly through altering the composition and functions of gut microbiota. In chapter 5, we investigated the cecal microbiota and egg production in two strains of Hy-Line (Brown and W-36) housed in conventional cages (CC) and enriched colony cages (EC), and noticed differences in egg production and cecal microbiota between strains and housing types. In chapter 6, we performed a comprehensive survey of the litter microbiotas using booty swab samples in the 5 commercial turkey farms of the Northwest Arkansas. The litter microbiotas were found to differ between farms, and flocks which were further affected by the ages of turkeys. In Chapter 7, we developed and evaluated the nested TaqMan probe based qPCR assay for the quantitative detection of Clostridium septicum that targets the alpha toxin gene (csa)

Community based seed production through IRRI /IAAS projects in western mid hills of Nepal: A review

A review on the community based seed production through IRRI/IAAS projects was made to know the seed production situation in western mid hills of Nepal. In Nepal, around 90% of seeds are supplied through informal sector where seed replacement rate (SRR) of major staple food crops is around 10%. The formal seed supply systems are characterized by a vertically organized production and distribution of released or registered varieties by public and private organizations using agreed quality control mechanism. Community Based Seed Production (CBSP) is one of the most important community resources from which, small holder farmers can improve their livelihoods which serves as a means to attain food and nutrition security and income generation to the farmers. IRRI/IAAS collaborative projects (IFAD TAG 706, STRASA and CURE) have establish 15 CBSP organizations in Lamjung, Tanahun, Gorkha and Bajhang district since 2006 to 2014 and producing quality seed annually. Project team has developed a unique type of working modality to get success in research, development and technology dissemination. Conduction of PVS trial annually in key sites, regular training to farmers, exposure visit of farmers, monthly meeting with farmers, regular minikit distribution in new sites, award to best seed producers, collaborative work with respective DADOs are some examples. Though CBSPs have faced major challenges like limitation of fund for seed buying, availability of quality source seed in time and place, availability of machines like moisture meter, grading machines, weighing machines, seed storage problems, marketing problems, natural disasters etc. the projects have contributed in release of rice varieties like Ghaiya-2, Sukhadhan series from 1 to 6, Bahuguni, Hardinath-2 and Chiyarang sub-1 as drought tolerant, and Ramdhan, Sunaulo sugandha, Mithila, Swarna sub 1 and Barkhe 1022 as irrigated varieties during project period

Performance of drought tolerant rice varieties in different altitudes at Duradada, Lamjung, Nepal

To evaluate high yielding drought tolerant rice varieties in different altitudes, a field experiment was carried out at Duradanda, Lamjung, Nepal during rainy season of 2016 under rainfed condition. The experiment was laid out in a factorial randomized block design with two replications. The treatments comprised of six different rice varieties viz. Sukhadhan-1, Sukhadhan-2, Sukhadhan-3, Sukhadhan-4, Sukhadhan-5 and Sukhadhan-6 and four altitudes viz. 1000, 1200, 1400 and 1600 masl. The results revealed that rice variety Sukhadhan-2 produced the highest grain yield (2.93 t/ha) followed by Sukhadhan-5 (2.92 t/ha) and Sukhadhan-3 (2.82 t/ha). The highest grain yield of Sukhadhan-2 was due to higher number of effective tillers/m2 (228), higher number of grains/panicle (83), higher panicle length (21.79 cm) and low sterility percentage (27.91%) as compared to other varieties. Regarding to altitudes, the highest grain yield (3.34 t/ha) was obtained in lower altitude (1000 masl) followed by 1200 masl (2.79 t/ha), 1400 masl (2.68 t/ha and 1600 masl (1.85 t/ha). The lower yield in higher altitude was due to higher sterility percentage, less number of effective tillers per unit area and less number of effective grains per panicle. It seems that the performance of these varieties was good up to 1400 masl

Gender inequality and the problem with girls' school attendance in Nepal : a qualitative perspective of patriarchal Nepalese practice

Masteroppgave i "Comparative social work" - Universitetet i Nordland, 201

Prokaryotes Rule the World

For millions of years, prokaryotic organisms have functioned as a vital selective force shaping eukaryotic evolution. It is now widely accepted that gut bacteria play a vital role in various physiological and metabolic activities of hosts, and thus, it is essential to maintain their homeostasis. Previous studies have shown an association of gut bacterial imbalance (dysbiosis) associated with several pathologies. However, very little is known about possible mechanisms involved between bacteria and hosts to maintain their homeostasis in the gut. Bacterial activities, such as cooperation (biofilm formation, horizontal gene transfer, quorum sensing, etc.), antagonism, and combination, and host responses of their immune system, gut barrier functions, and different dietary components have been identified as crucial factors for maintaining bacterial homeostasis in the gut. Our understanding of several possible mechanisms involved in gut bacterial homeostasis should be widened to modulate their composition or treat diseases. The objective of this chapter is to provide an overview of different factors involved in gut bacterial homeostasis with an emphasis on host intestinal barrier and immune system, dietary components, and quorum sensing. Also, brief information regarding roles of microbiota on gut-brain axis has also been included

Genetic variability, heritability, genetic advance and correlation among yield and yield components of rice (Oryza sativa L.)

This study was conducted during summer 2015 at Regional Agriculture Research Station, Dipayal, Doti, Nepal to estimate the genotypic and phenotypic variability, heritability, genetic advance and correlation on grain yield and yield associated traits using 26 advance genotypes of lowland irrigated rice. Analysis of variance revealed the existence of significant difference for days to flowering, maturity, plant height, panicle length, thousand grain weight and grain yield. High heritability was estimated for days to flowering (0.88), maturity (0.79), thousand grain weight (0.48) and plant height (0.43) suggesting these traits are under high genetic control. High phenotypic variation was observed for grain yield (24.87%), number of grains/panicle (22.45%), number of panicles/m2 (20.95%) and straw yield (20.75%) while grain yield had medium (12.02%) and remaining traits showed low genotypic coefficient of variation (<10%). High phenotypic coefficient of variation estimated as compared to genotypic coefficient of variation showed environmental influence on the expression of traits. Grain yield (11.98) and days to flowering (10.32) showed medium and remaining traits sowed low genotypic advance as percent of mean. High to low heritability with moderate to low genotypic advance as percent of mean suggested these traits were governed by non additive gene thus direct selection is not beneficial. Further improvements on yield potentiality and yield traits on these genotypes are suggested by creating variation and selection. Panicle length (r = 0.230), days to flowering (r = 0.247), effective tillers (r = 0.488) and straw yield (r = 0.846) manifested significant positive association with grain yield indicating that yield can be increased if selection applied in favor of those yield components

Good agricultural practices in mandarin (Citrus reticulata Blanco); Perception and factors affecting awareness among farmers in Gulmi, Nepal

Citrus, in general, and mandarin, in particular, has long been one of Nepal's most important fruit crops. However, in recent years, a lack of good cultivation procedures and negligence has resulted in a significant reduction in its productivity. The declining situation cannot be controlled without the transfer of science-based knowledge and skills where Good Agricultural Practices have been linked to higher output. A study was carried out in 2020 to assess the perception of farmers towards Good Agricultural Practices and factors affecting awareness of GAP among mandarin growers. Altogether 100 households from Dhurkot and Chhatrakot Rural Municipalities, and Resunga Municipality from Gulmi district, were purposively chosen for the study in the command area of the PMAMP PIU Citrus Zone, and sampling was carried out using a simple random sampling technique. Data were collected with the use of a semi-structured questionnaire, entered, and analyzed using SPSS and STATA software. The farmers were categorized into GAP aware and unaware based on the criteria made by the focus group discussion carried out with the leading farmers, mandarin experts, and government agriculture officers. The findings revealed that 39% of the total respondents knew about GAP for mandarin. The entire respondents agreed that GAP-produced fruit has a nicer appearance and better quality and fruits meet national and international standards and enhance exportability of fruits, however, 61.5% of respondents didn’t agree that GAP helps in providing subsidies from governmental organizations. The output of the binary logit model suggested that an increase in participation in training and contact with extension agents has a significant effect on awareness of GAP. Participatory GAP training, regular extension services to smallholding farmers could be beneficial for mandarin farmers leading to commercialization

Modifying and validating the Composite International Diagnostic Interview (CIDI) for use in Nepal

Background : Efforts to develop and validate fully‐structured diagnostic interviews of mental disorders in non‐Western countries have been largely unsuccessful. However, the principled methods of translation, harmonization, and calibration that have been developed by cross‐national survey methodologists have never before been used to guide such development efforts. The current report presents the results of a rigorous program of research using these methods designed to modify and validate the Composite International Diagnostic Interview (CIDI) for an epidemiological survey in Nepal. Methods : A five‐step process of translation, harmonization, and calibration was used to modify the instrument. A blinded clinical reappraisal design was used to validate the instrument. Results : Preliminary interviews with local mental health expert led to a focus on major depressive episode, mania/hypomania, panic disorder, post‐traumatic stress disorder, generalized anxiety disorder, and intermittent explosive disorder. After an iterative process of multiple translations‐revisions guided by the principles developed by cross‐national survey methodologists, lifetime DSM‐IV diagnoses based on the final Nepali CIDI had excellent concordance with diagnoses based on blinded Structured Clinical Interview for DSM‐IV (SCID) clinical reappraisal interviews. Conclusions : Valid assessment of mental disorders can be achieved with fully‐structured diagnostic interviews even in low‐income non‐Western settings with rigorous implementation of replicable developmental strategies. Copyright © 2013 John Wiley & Sons, Ltd.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/97206/1/mpr1375.pd

Some Medicinal Plants Uses in Ethnical Group from Biratnagar, Eastern, Nepal

This paper aims to explore of medicinal plants and their uses for the local communities in Biratnagar Nepal. We have explained here 32 medicinal plants with their habitat and part used for medicinal uses. The plants were Collected through the direct visit in a different interval of time after that plants were identified 32 species were described as their medicinal value with their plant Parts.  The used of medicinal value of the medicinal plant is great Knowledge of ethnical society

Geomorphological and hydrological controls on sediment export in earthquake-affected catchments in the Nepal Himalaya

Large earthquakes can contribute to mountain growth by building topography but also contribute to mass removal from mountain ranges through widespread mass wasting. On annual to decadal or centennial timescales, large earthquakes also have the potential to significantly alter fluvial sediment dynamics if a significant volume of the sediment generated reaches the fluvial network. In this contribution, we focus on the Melamchi–Indrawati and Bhote Koshi rivers in central Nepal, which have both experienced widespread landsliding associated with the 2015 Gorkha (Nepal) earthquake. Using a time series of high-resolution satellite imagery, we have mapped exposed sediment along the rivers from 2012–2021 to identify zones of active channel deposition and document changes over time. Counter to expectations, we show negligible increases in coarse-sediment accumulation along both river corridors since the Gorkha earthquake. However, an extremely high-concentration flow event on 15 June 2021 caused an approximately 4-fold increase in exposed sediment along a 30 km reach of the channel with up to 12 m of channel aggradation in the Melamchi–Indrawati rivers; this event was localised and did not impact the neighbouring Bhote Koshi catchment. Based on published reports, new helicopter-based photography, and satellite data, we demonstrate that this event was sourced from a localised rainfall event between 4500 and 4800 m and that a significant fraction of the sediment was supplied from sources that were unrelated to the landslides generated by the Gorkha earthquake.</p