Key Predictors of Well-Being for Individuals with Dementia

Until recently, few studies have investigated the psychosocial outcomes of individuals living with dementia. More specifically, the relationship among depression, anxiety, and quality of life as well-being outcomes. The current study examined the role of well-being outcomes and how key predictors (level of cognitive impairment, level of distress experienced, relationship role strain) influenced these outcomes. It addressed how these specific predictors had an impact, on depression and anxiety symptoms and overall quality of life. This study utilized the stress process model for individuals with dementia as a framework to demonstrate the intricacies of the illness experience for the individuals. Self-report data was used from IWDs (N = 131) about their illness experienc

Salish Sea bull kelp restoration research: local, regional and international collaborations

Bull kelp forests are important nearshore habitats for many fish and invertebrates and are an integral part of the “salmon highway” from river to estuary to ocean and back. In recent decades kelp forests have been in serious decline in the Salish Sea and other coastal regions. Research to improve our understanding of causes of the decline and on restoration methods began in the north Salish Sea in 2011 with a kelp out-planting project by Nile Creek Enhancement Society(NCES) at Hornby Island. NCES and Project Watershed began a collaboration in 2015 with work in the K\u27omoks Estuary and Cape Lazo shoal, becoming part of the Salish Sea Marine Survival Project of the Pacific Salmon Foundation. In 2016 academic collaborations with Simon Fraser University and with the University of Wisconsin – Milwaukee were initiated to include the study of stress-resiliency of life stages and population genetics of bull kelp. Project Watershed and NCES also participate in a Nearshore Habitat Working Group in BC with members from Citizen\u27s Science, three universities and several non-profit environmental groups

Alignment of Centrosomal and Growth Axes Is a Late Event during Polarization ofPelvetia compressaZygotes

AbstractZygotes and embryos of the fucoid brown algaPelvetia compressaundergo a series of asymmetric cleavages. We are interested in the developmental role of these cleavages and the mechanism controlling their alignment. To assess the importance of division plane alignment, the orientation of the first asymmetric division was altered and the effects on subsequent embryo elongation rates were analyzed. Although this division is normally oriented transversely (90°) to the growth axis, deviations up to 45° had no significant effects on embryo elongation. However, division planes that were parallel with the growth axis (0–45°) had drastic effects. Embryo elongation was severely inhibited and the wall often bifurcated and avoided the rhizoid tip. The orientation of the division plane is determined by the position of the centrosomes. We therefore investigated centrosomal position and function during the first cell cycle within the three-dimensional context of the cell. We found that, after karyogamy, microtubule organization changed from a radially symmetric circumnuclear array into a bipolar centrosomal array. The reorganization coincided with the migration of the centrosomes around the nucleus. The centrosomes separated slowly and asynchronously until they reached opposite sides of the nuclear envelope. At this time the centrosomal axis, defined by the position of the two centrosomes, was oriented randomly with respect to the cortical growth axis. The centrosomal axis then rotated into alignment parallel with the growth axis late in the first cell cycle. These results indicate that the growth axis and the centrosomal axis develop independently of each other and that the centrosomal axis does not align with the growth axis until just prior to mitosis

Arabidopsis TAO1 is a TIR-NB-LRR protein that contributes to disease resistance induced by the Pseudomonas syringae effector AvrB

The type III effector protein encoded by avirulence gene B (AvrB) is delivered into plant cells by pathogenic strains of Pseudomonas syringae. There, it localizes to the plasma membrane and triggers immunity mediated by the Arabidopsis coiled-coil (CC)-nucleotide binding (NB)-leucine-rich repeat (LRR) disease resistance protein RPM1. The sequence unrelated type III effector avirulence protein encoded by avirulence gene Rpm1 (AvrRpm1) also activates RPM1. AvrB contributes to virulence after delivery from P. syringae in leaves of susceptible soybean plants, and AvrRpm1 does the same in Arabidopsis rpm1 plants. Conditional overexpression of AvrB in rpm1 plants results in leaf chlorosis. In a genetic screen for mutants that lack AvrB-dependent chlorosis in an rpm1 background, we isolated TAO1 (target of AvrB operation), which encodes a Toll-IL-1 receptor (TIR)-NB-LRR disease resistance protein. In rpm1 plants, TAO1 function results in the expression of the pathogenesis-related protein 1 (PR-1) gene, suggestive of a defense response. In RPM1 plants, TAO1 contributes to disease resistance in response to Pto (P. syringae pathovars tomato) DC3000(avrB), but not against Pto DC3000(avrRpm1). The tao1–5 mutant allele, a stop mutation in the LRR domain of TAO1, posttranscriptionally suppresses RPM1 accumulation. These data provide evidence of genetically separable disease resistance responses to AvrB and AvrRpm1 in Arabidopsis. AvrB activates both RPM1, a CC-NB-LRR protein, and TAO1, a TIR-NB-LRR protein. These NB-LRR proteins then act additively to generate a full disease resistance response to P. syringae expressing this type III effector

Multiple Roles of Pitx2 in Cardiac Development and Disease

Cardiac development is a complex morphogenetic process initiated as bilateral cardiogenic mesoderm is specified at both sides of the gastrulating embryo. Soon thereafter, these cardiogenic cells fuse at the embryonic midline configuring a symmetrical linear cardiac tube. Left/right bilateral asymmetry is first detected in the forming heart as the cardiac tube bends to the right, and subsequently, atrial and ventricular chambers develop. Molecular signals emanating from the node confer distinct left/right signalling pathways that ultimately lead to activation of the homeobox transcription factor Pitx2 in the left side of distinct embryonic organ anlagen, including the developing heart. Asymmetric expression of Pitx2 has therefore been reported during different cardiac developmental stages, and genetic deletion of Pitx2 provided evidence of key regulatory roles of this transcription factor during cardiogenesis and thus congenital heart diseases. More recently, impaired Pitx2 function has also been linked to arrhythmogenic processes, providing novel roles in the adult heart. In this manuscript, we provide a state-of-the-art review of the fundamental roles of Pitx2 during cardiogenesis, arrhythmogenesis and its contribution to congenital heart diseases

Effects of warm ocean temperatures on bull kelp forests in the Salish Sea

Kelp beds are marine sanctuaries, providing some of the most productive ecosystems on the planet and serving as critical habitat and refuge for many species, including juvenile salmon. Rising ocean temperature associated with climate change is a major stressor contributing to declines of kelp forests worldwide. In the Salish Sea, we identified bull kelp (Nereocystis leutkeana) populations growing under two different temperature regimes. Since 2011, kelp growing in the central Strait of Georgia has been exposed to sea surface temperatures (SSTs) of 15-21 °C in the summer months, which is 5-6 °C warmer than temperatures in the Strait of Juan De Fuca. To assess the effects of high summer SSTs, populations growing at a warm and a cold site (Stanley Park and French Beach respectively) were sampled in the summer and fall of 2017. Warmer summer SSTs at Stanley Park correlated with significant declines in spore production, indicating negative effects on the reproductive capacity of that population. Growth and development of healthy spores from either population was reduced when incubated in the lab at temperatures above ~ 17 °C and temperatures of ~ 20 °C resulted in mortality of nearly all spores. Analyses of stress levels in spores exposed to different temperatures, based on the production of reactive oxygen species, is now underway. This research continues to provide crucial data needed for habitat restoration efforts as they will allow for the selection of stress-resilient kelp stocks that are better adapted for survival in warmer oceans

Maintenance of genetic variation in plants and pathogens involves complex networks of gene-for-gene interactions

The RPP13 [recognition of Hyaloperonospora arabidopsidis (previously known as Peronospora parasitica)] resistance (R) gene in Arabidopsis thaliana exhibits the highest reported level of sequence diversity among known R genes. Consistent with a co-evolutionary model, the matching effector protein ATR13 (A. thaliana-recognized) from H. arabidopsidis reveals extreme levels of allelic diversity. We isolated 23 new RPP13 sequences from a UK metapopulation, giving a total of 47 when combined with previous studies. We used these in functional studies of the A. thaliana accessions for their resistance response to 16 isolates of H. arabidopsidis. We characterized the molecular basis of recognition by the expression of the corresponding ATR13 genes from these 16 isolates in these host accessions. This allowed the determination of which alleles of RPP13 were responsible for pathogen recognition and whether recognition was dependent on the RPP13/ATR13 combination. Linking our functional studies with phylogenetic analysis, we determined that: (i) the recognition of ATR13 is mediated by alleles in just a single RPP13 clade; (ii) RPP13 alleles in other clades have evolved the ability to detect other pathogen ATR protein(s); and (iii) at least one gene, unlinked to RPP13 in A. thaliana, detects a different subgroup of ATR13 allele

Systems Analysis Implicates WAVE2 Complex in the Pathogenesis of Developmental Left-Sided Obstructive Heart Defects.

Genetic variants are the primary driver of congenital heart disease (CHD) pathogenesis. However, our ability to identify causative variants is limited. To identify causal CHD genes that are associated with specific molecular functions, the study used prior knowledge to filter de novo variants from 2,881 probands with sporadic severe CHD. This approach enabled the authors to identify an association between left ventricular outflow tract obstruction lesions and genes associated with the WAVE2 complex and regulation of small GTPase-mediated signal transduction. Using CRISPR zebrafish knockdowns, the study confirmed that WAVE2 complex proteins brk1, nckap1, and wasf2 and the regulators of small GTPase signaling cul3a and racgap1 are critical to cardiac development

Wdr18 Is Required for Kupffer's Vesicle Formation and Regulation of Body Asymmetry in Zebrafish

Correct specification of the left-right (L-R) axis is important for organ morphogenesis. Conserved mechanisms involving cilia rotation inside node-like structures and asymmetric Nodal signaling in the lateral plate mesoderm (LPM), which are important symmetry-breaking events, have been intensively studied. In zebrafish, the clustering and migration of dorsal forerunner cells (DFCs) is critical for the formation of the Kuppfer's vesicle (KV). However, molecular events underlying DFC clustering and migration are less understood. The WD-repeat proteins function in a variety of biological processes, including cytoskeleton assembly, intracellular trafficking, mRNA splicing, transcriptional regulation and cell migration. However, little is known about the function of WD-repeat proteins in L-R asymmetry determination. Here, we report the identification and functional analyses of zebrafish wdr18, a novel gene that encodes a WD-repeat protein that is highly conserved among vertebrate species. wdr18 was identified from a Tol2 transposon-mediated enhancer trap screen. Follow-up analysis of wdr18 mRNA expression showed that it was detected in DFCs or the KV progenitor cells and later in the KV at early somitogenesis stages. Morpholino knockdown of wdr18 resulted in laterality defects in the visceral organs, which were preceded by the mis-expression of Nodal-related genes, including spaw and pitx2. Examination of morphants at earlier stages revealed that the KV had fewer and shorter cilia which are immotile and a smaller cavity. We further investigated the organization of DFCs in wdr18 morphant embryos using ntl and sox17 as specific markers and found that the clustering and migration of DFC was altered, leading to a disorganized KV. Finally, through a combination of wdr18 and itgb1b morpholino injections, we provided evidence that wdr18 and itgb1b genetically interact in the laterality determination process. Thus, we reveal a new and essential role for WD-repeat proteins in the determination and regulation of L-R asymmetry and propose a potential mechanism for wdr18 in the regulation of DFC clustering and migration and KV formation