56 research outputs found
Inhaled and intravenous application of a stimulator of the soluble guanylate cyclase (BAY 41-8543) reduces pulmonary vascular resistance in a model of septic shock
Revealing the Compact Structure of Lactic Acid Bacterial Heteroexopolysaccharides by SAXS and DLS
Molecular structures
of exopolysaccharides are required to understand
their functions and the relationships between the structure and physical
and rheological properties. Small-angle X-ray scattering and dynamic
light scattering were used in conjunction with molecular modeling
to characterize solution structures of three lactic acid bacterial
heteroexopolysaccharides (HePS-1, HePS-2, and HePS-3). Values of radius
of gyration <i>R</i><sub>G</sub>, cross-sectional radius
of gyration <i>R</i><sub>XS</sub>, approximate length <i>L</i>, and hydrodynamic diameter were not directly proportional
to the molar mass and indicated the HePSs adopted a compact coil-like
rather than an extended conformation. Constrained molecular modeling
of 15000 randomized HePS-1 conformers resulted in five best-fit structures
with <i>R</i> factor of 3.9â4.6% revealing random
coil-like structure. Ί and Κ angle analysis of glycosidic
linkages in HePS-1 structures suggests Gal<i>f</i> residues
significantly influence the conformation. Ab initio scattering modeling
of HePS-2 and HePS-3 gave excellent curve fittings with Ï<sup>2</sup> of 0.43 and 0.34 for best-fit models, respectively, compatible
with coil-like conformation. The findings disclose solution behavior
of HePS relevant for their interactions with biomacromolecules, for
example, milk proteins
Interaction between ÎČ-lactoglobulin and structurally different heteroexopolysaccharides investigated by solution scattering and analytical ultracentrifugation study
Insect biomass shows a stronger decrease than species richness along urban gradients
1. Anthropogenic land cover change is a major driver of biodiversity loss, with urbanisation and farmland practices responsible for some of the most drastic modifications of natural habitats. The relative importance of different land covers for shaping insect communities, however, is unclear.
2. This study examines the effect of urban and farmland covers, along with land cover heterogeneity, at a landscape scale on species richness, evenness and biomass of flying insects using citizen science carnet sampling across Denmark.
3. Increasing urban cover had a negative effect on insect richness but an even stronger negative effect on biomass. Increased land cover heterogeneity did not mitigate the negative effect of urban cover. Insect assemblages also became more even with increased urban cover. Farmland cover had no significant effect on insect richness, evenness or biomass.
4. Based on our findings, the urban cover has a strong negative impact on insect communities, indicating that urbanisation could contribute to insect declines. Moreover, our findings indicate that insect loss occurs more through loss of biomass than loss of species, which may affect the ecosystem-level consequences of urbanisation
Interaction between structurally different heteroexopolysaccharides and ÎČ-lactoglobulin studied by solution scattering and analytical ultracentrifugation
Predmet ovog rada je Lean proizvodnja-korak ka uspjehu. Lean proizvodnje naglaĆĄava
minimiziranje koliÄine resursa koji se koriste u raznim aktivnostima kompanije i ukljuÄuje
identificiranje i eliminiranje aktivnosti koje ne donosi vrijednost. Filozofija Lean proizvodnje
ukljuÄuje principe i praksu smanjivanja troĆĄkova kroz uklanjanje "otpada" i kroz
pojednostavljenje svih aktivnosti kompanije. Postoji sedam vrsta tradicionalnih otpada u
aktivnostima kompanije i viĆĄe od desetak alata za njihovo uklanjanje. U postizanju filozofije
Lean proizvodnje pet je glavnih koraka koji oznaÄavaju ĆŸivotni ciklus racionalne proizvodnje.
Lean proizvodnja i filozofija racionalnog poslovanja omoguÄava kompanijama da budu bolje,
brĆŸe, njihovi proizvodi i/ili usluge jeftinije, a one same privlaÄnije kupcima.The subject of this paper is Lean production-step to success. Lean Production highlights the minimization of the amount of resources used in the company's various activities and involves identifying and eliminating activities that do not bring value. The Lean manufacturing philosophy includes the principles and practice of reducing costs through the removal of
"waste" and simplifying all company activities. There are seven types of traditional waste in the company's activities and more than a dozen tools for their removal. In achieving the Lean production philosophy, five major steps are to be found that indicate the life cycle of rational production. Lean manufacturing and the philosophy of rational business enable companies to be better, faster, their products and/or services cheaper, and those more attractive to customers
Interaction between structurally different heteroexopolysaccharides and ÎČ-lactoglobulin studied by solution scattering and analytical ultracentrifugation
Autochthonous heritage languages and social media:writing and bilingual practices in Low German on Facebook
This article analyses how speakers of an autochthonous heritage language (AHL) make use of digital media, through the example of Low German, a regional language used by a decreasing number of speakers mainly in northern Germany. The focus of the analysis is on Web 2.0 and its interactive potential for individual speakers. The study therefore examines linguistic practices on the social network site Facebook, with special emphasis on language choice, bilingual practices and writing in the autochthonous heritage language. The findings suggest that social network sites such as Facebook have the potential to provide new mediatized spaces for speakers of an AHL that can instigate sociolinguistic change
Inactivation of murine norovirus by chemical biocides on stainless steel
<p>Abstract</p> <p>Background</p> <p>Human norovirus (NoV) causes more than 80% of nonbacterial gastroenteritis in Europe and the United States. NoV transmission via contaminated surfaces may be significant for the spread of viruses. Therefore, measures for prevention and control, such as surface disinfection, are necessary to interrupt the dissemination of human NoV. Murine norovirus (MNV) as a surrogate for human NoV was used to study the efficacy of active ingredients of chemical disinfectants for virus inactivation on inanimate surfaces.</p> <p>Methods</p> <p>The inactivating properties of different chemical biocides were tested in a quantitative carrier test with stainless steel discs without mechanical action. Vacuum-dried MNV was exposed to different concentrations of alcohols, peracetic acid (PAA) or glutaraldehyde (GDA) for 5 minutes exposure time. Detection of residual virus was determined by endpoint-titration on RAW 264.7 cells.</p> <p>Results</p> <p>PAA [1000 ppm], GDA [2500 ppm], ethanol [50% (v/v)] and 1-propanol [30% (v/v)] were able to inactivate MNV under clean conditions (0.03% BSA) on the carriers by â„ 4 log<sub>10 </sub>within 5 minutes exposure time, whereas 2-propanol showed a reduced effectiveness even at 60% (v/v). Furthermore, there were no significant differences in virus reduction whatever interfering substances were used. When testing with ethanol, 1- and 2-propanol, results under clean conditions were nearly the same as in the presence of dirty conditions (0.3% BSA plus 0.3% erythrocytes).</p> <p>Conclusion</p> <p>Products based upon PAA, GDA, ethanol and 1-propanol should be used for NoV inactivation on inanimate surfaces. Our data provide valuable information for the development of strategies to control NoV transmission via surfaces.</p
Occupational accident and disease claims, work-related stress and job satisfaction of physiotherapists
Infektionsquellensuche bei ambulant erworbenen FÀllen von LegionÀrskrankheit
Bei den meisten FĂ€llen von ambulant erworbener LegionĂ€rskrankheit (AE-LK) gelingt es auch in interÂŹnationalen Studien nicht, die verantwortliche InfekÂŹtionsquelle nachzuweisen. Ein Ziel der Berliner LeTriWa-Studie (âLegionellen in der Trinkwasser-Installationâ) war es, herauszufinden, bei wie vielen FĂ€llen evidenzbasiert eine Infektionsquelle identifiÂŹziert werden kann. Dazu wurden im Zeitraum 2016 bis 2020 FĂ€lle von AE-LK und Kontrollpersonen rekrutiert, Urin- und tiefe Atemwegsproben untersucht und Befragungen zu potenziellen Expositionen durchgefĂŒhrt. Zudem wurden verschiedene hĂ€usliche und auĂerhĂ€usliche Infektionsquellen beprobt. Die Zuordnung der potenziellen Infektionsquelle erfolgte mittels einer eigens entwickelten Evidenz-Matrix. Im vorliegenden Teil 1 des Berichts werden zunĂ€chst die HintergrĂŒnde, Ziele und Methoden der LeTriWa-Studie vorgestellt.Peer Reviewe
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