Identification of a Phytase Gene in Barley (Hordeum vulgare L.)

Background: Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity. Methodology/Principal Findings: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy. Conclusions/Significance: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains

Beneficial Effects of Probiotic and Food Borne Yeasts on Human Health

Besides being important in the fermentation of foods and beverages, yeasts have shown numerous beneficial effects on human health. Among these, probiotic effects are the most well known health effects including prevention and treatment of intestinal diseases and immunomodulatory effects. Other beneficial functions of yeasts are improvement of bioavailability of minerals through the hydrolysis of phytate, folate biofortification and detoxification of mycotoxins due to surface binding to the yeast cell wall

Improvement of chemical properties of noodle and pasta using dephytinized cereal brans

WOS:000540290000043In this research, cereal brans (rice, rye, wheat and oat) were dephytinized by two different methods (phytase enzyme and malt flour) and used in noodle and pasta formulation (20%) to improve nutritional quality of the final product. As a result of phytase treatment, phytic acid content of different bran samples decreased between 70.9 and 83.5%. Rice bran noodles and pasta samples gave the highest amount of phytic acid. In general, the use of cereal bran in noodles samples increased protein and fat content compared to control noodles. The use of cereal bran increased the total dietary fiber content by 1.9-3.3 times in noodle and 1.7-2.9 times in pasta samples. All bran samples increased total phenolic content and antioxidant activity values in noodles and pasta samples (p < 0.05). The amount of Ca, P, K, Mg and Zn of cereal bran noodles and pasta samples was higher than control noodles and pasta samples (p < 0.05). In addition to nutritional enrichment, it was concluded that the noodles and pasta samples with a lower phytic acid content can be produced using dephytinized cereal brans.This study was supported by the Turkish Scientific and Technological Research Council (TUBITAK) with the project no '118O385'

Synthesis, characterization, biological evaluation, and molecular docking studies of some piperonyl-based 4-thiazolidinone derivatives

PMID = 3177798

Optoelectronic parameters of peripherally tetra-substituted copper(ii) phthalocyanines and fabrication of a photoconductive diode for various conditions

In this study, the molecular structure of 4-(4-(trifluoromethoxy)phenoxy)phthalonitrile (1) has been elucidated and its supra-molecular dynamics have been revealed by the analysis of single crystal X-ray diffraction measurements. Compound (1) and its copper(ii) phthalocyanine (2) were theoretically calculated with different basis sets. The Gibbs free energy values of copper(ii) phthalocyanine (2) were investigated. The calculated values of IR, NMR and UV-vis spectra for compound (1) and its copper(ii) phthalocyanine (2) were compared with the experimentally obtained values. The surface morphology properties of the compound (2) film for different magnifications were examined with its SEM images. We investigated the optical and optoelectronic parameters of compound (2) for different molarities. Also, we fabricated a diode based on compound (2) and examined its electronic characteristics under darkness, room light and 100 mW cm(-2). Our diode exhibits a photoconductive property

Novel propanolamine derivatives attached to 2-metoxifenol moiety: Synthesis, characterization, biological properties, and molecular docking studies

PMID = 3247418