Comparison of a rapid, quantitative and automated assay for urinary luteinizing hormone (LH), with an LH detection test, for the prediction of ovulation

The prediction of ovulation is necessary for oocyte aspiration in a spontaneous cycle and can be reliably achieved only by measuring luteinizing hormone (LH). Since radioimmunoassays of LH take too long for repeated measurements on the same day, we evaluated the possibility of adapting a rapid and fully automated assay of serum LH for use with urine samples. The study group comprised spontaneously ovulating women (38 cycles) who requested artificial insemination. Their serum oestradiol (E2) levels, ultrasound profile (US) and thrice daily urinary LH levels were determined from day 10 of their menstrual cycle. These patients were followed until US signs of follicular rupture were recorded. In all patients, a well-defined LH peak was measured in the urine. This peak lasted 12-15 h and was followed in 35 cycles (no US available for 3) by follicular rupture 9-51 h later. The data were grouped according to the time of the LH peak on day 0. Patients experiencing an LH peak between 0300 h and 0700 h on day 0 had significantly lower levels of E2 on day 0 compared to those with an LH peak between 2200 h and midnight. This is due to the fact that in the patients with an LH peak between 0300 h and 0700 h, E2 levels were already decreasing (from day 1 to day 0), whereas in those with the LH peak between 2200 h and midnight E2 levels were still increasing on the morning of day 0. We conclude that the 30-min IMX LH assay is a reliable, rapid and readily acceptable method for measuring urinary LH and for the prediction of ovulatio

Fertilization and early embryology: Evidence of sperm entry into assumed unfertilized human oocytes after sub-zonal sperm microinjection

Sub-zonal sperm microinjection (SUZI) as a treatment for male factor infertility can facilitate fertilization, however, in many cases oocytes remain unfertilized even though the sperm is placed in close contact with the oolemma. In order to improve our understanding of gamete interaction in cases of failed fertilization, we have analysed the failed fertilized oocytes from both SUZI and conventional in-vitro fertilization. The fluorochrome Hoechst 33342 (which binds specifically to DNA) was used to check for the possible presence of paternal chromatin in the unfertilized oocytes. A significantly higher (P < 0.01) number of microinjected oocytes showed signs of fertilization 2-3 days after sperm microinjection compared to normally inseminated oocytes, 30/175 (17.1%) and 2/79 (2.5%) respectively. In addition, four out of eight couples returning for a second treatment by SUZI displayed anomalies in fertilization in both cycles. The semen characteristics of patients with or without anomalies in fertilization was not different. The irregularities observed in the fertilization process infer that certain male factor patients have intrinsic sperm anomalies lying at the sperm membrane and/or chromatin level that could lead to anomalies in the appearance of the pronucle

Non-Fermi Liquid behavior in CeIrIn5_5 near a metamagnetic transition

We present specific heat and resistivity study of CeIrIn5 in magnetic fields up to 17 T and temperature down to 50 mK. Both quantities were measured with the magnetic field parallel to the c-axis (H || [001]) and within the a-b plane (H \perp [001]). Non-Fermi-liquid (NFL) behavior develops above 12 T for H || [001]. The Fermi liquid state is much more robust for H \perp [001] and is suppressed only moderately at the highest applied field. Based on the observed trends and the proximity to a metamagnetic phase transition, which exists at fields above 25 T for H || [001], we suggest that the observed NFL behavior in CeIrIn5 is a consequence of a metamagnetic quantum critical point.Comment: 5 pages, 4 figures, submitted to Phys. Rev. Letter

Sperm nuclear DNA damage and altered chromatin structure: effect on fertilization and embryo development

In the first part of this report we investigate whether chromatin anomalies in human spermatozoa can influence fertilization after intracytoplasmic sperm injection (ICSI). We have examined the sperm chromatin packaging quality using the chromomycin A3 (CMA3) fluorochrome and the presence of DNA damage in spermatozoa using in-situ nick translation. When comparing the spermatozoa of patients undergoing in-vitro fertilization (IVF) and ICSI distinct differences are evident in that ICSI males have a higher CMA3 fluorescence, indicating spermatozoa with loosely packed chromatin, and more spermatozoa containing endogenous DNA nicks. When examining the unfertilized oocytes of ICSI patients we found that men who had a high percentage of anomalies in their chromatin, i.e. >30% CMA3 fluorescence and >10% nicks, had more than double the number of unfertilized oocytes containing spermatozoa that had remained condensed. The observation that failed fertilized oocytes, injected with spermatozoa from patients with a higher percentage of sperm nuclear anomalies, contain more condensed spermatozoa indicates that a selection process against these spermatozoa may be in place at the time of fertilization. In the second part of the study we show that spare ICSI embryos have significantly lower rates of development to the blastocyst stage compared with those developed after routine IVF. These results show that a greater understanding of the molecular basis of male infertility is therefore needed to broaden our knowledge on the effect that abnormal spermatozoa have on fertilization and embryo developmen

Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection

In this study we investigated whether morphology and chromatin anomalies in human spermatozoa can influence fertilization after intracytoplasmic sperm injection (ICSI). We examined unfertilized oocytes, using the fluorochrome Hoechst 33342, to determine whether a relationship exists between failure of fertilization and sperm chromatin quality. Sperm chromatin packaging quality was assessed using the chromomydn A3 (CMA3) fluorochrome, and the presence of DNA damage in spermatozoa, using in-situ nick translation. Normal males present sperm parameters with a normal morphology of >20%, CMA3 fluorescence of <30% and exhibit endogenous nicks in <10% of their spermatozoa. When patients were separated according to these values no difference was observed in their fertilization rates after ICSL When the unfertilized ICSI oocytes were examined, we found that patients with CMA3 fluorescence of <30% and nicks in <10% of their spermatozoa had only 17.5 and 21.6% respectively of their unfertilized oocytes containing spermatozoa that remained condensed. In contrast, patients with higher CMA3 and nick values had a significantly higher number, 412 and 48.9%, of their unfertilized oocytes containing condensed spermatozoa. Sperm morphology did not show any such pattern. The percentage of spermatozoa which had initiated decondensation in unfertilized oocytes was not influenced by morphology, CMA3 fluorescence or nicks. In light of these results we postulate that poor chromatin packaging and/or damaged DNA may contribute to failure of sperm decondensation after ICSI and result in failure of fertilizatio

String instantons, fluxes and moduli stabilization

We analyze a class of dual pairs of heterotic and type I models based on freely-acting $\mathbb{Z}_2 \times \mathbb{Z}_2$ orbifolds in four dimensions. Using the adiabatic argument, it is possible to calculate non-perturbative contributions to the gauge coupling threshold corrections on the type I side by exploiting perturbative calculations on the heterotic side, without the drawbacks due to twisted moduli. The instanton effects can then be combined with closed-string fluxes to stabilize most of the moduli fields of the internal manifold, and also the dilaton, in a racetrack realization of the type I model.Comment: 1+49 page

Evolution From Insulator (x=0.003) To Metal (x=1) Of The Eu 2+ Local Environment In Ca 1-xeu Xb 6

The local environment of Eu2+ (4 f7, S=72) in Ca1-x Eux B6 (0.003≤x≤1.00) is studied by means of electron spin resonance (ESR). For x≲0.07 the resonances have Lorentzian line shape, indicating an insulating environment for the Eu2+ ions. For x≳0.07, the lines broaden and become Dysonian in shape, suggesting a change to metallic environment for the Eu2+ ions, anticipating the semimetallic character of EuB6. The broadening is attributed to a spin-flip scattering relaxation process due to the exchange interaction between conduction and Eu2+ 4f electrons. High field ESR measurements for x≳0.30 reveal narrower and anisotropic linewidths, which are attributed to magnetic polarons and Fermi surface effects, respectively. © 2005 American Institute of Physics.9710Young, D.P., (1999) Nature (London), 397, p. 412Zhitomirsky, M.E., (1999) Nature (London), 402, p. 251Tromp, H.J., (2000) Phys. Rev. Lett., 87, p. 016401Massidda, S., Continenza, A., De Pascale, T.M., Monnier, R., (1997) Z. Phys. B: Condens. Matter, 102, p. 83Urbano, R.R., (2002) Phys. Rev. B, 65, p. 180407Bennett, M.C., (2004) Phys. Rev. B, 69, p. 132407Urbano, R.R., Pagliuso, P.G., Rettori, C., Oseroff, S.B., Sarrao, J.L., Schlottmann, P., Fisk, Z., (2004) Phys. Rev. B, 70, p. 140401Pake, G.E., Purcell, E.M., (1948) Phys. Rev., 74, p. 1184Bloembergen, N., (1952) J. Appl. Phys., 23, p. 1383Feher, G., Kip, A.F., (1955) Phys. Rev., 98, p. 337Dyson, F.J., (1955) Phys. Rev., 98, p. 349Sperlich, G., Jansen, K., (1974) Solid State Commun., 15, p. 1105Essam, J.W., (1972) Phase Transitions and Critical Phenomena, 2, p. 197. , Academic, LondonSchlottmann, P., Hellberg, C.S., (1996) J. Appl. Phys., 79, p. 6414Fisk, Z., (1979) J. Appl. Phys., 50, p. 1911Goodrich, R.G., Harrison, N., Vuillemin, J.J., Tekul, A., Hall, D.W., Fisk, Z., Young, D., Sarrao, J., (1998) Phys. Rev. B, 58, p. 14896Rhyee, J.-S., Cho, B.K., Ri, H.-C., (2003) Phys. Rev. B, 67, p. 125102Wigger, G.A., Beeli, C., Felder, E., Ott, H.R., Bianchi, A.D., Fisk, Z., (2004) Phys. Rev. Lett., 93, p. 14720

Unoriented D-brane Instantons vs Heterotic worldsheet Instantons

We discuss Fermi interactions of four hyperini generated by ``stringy'' instantons in a Type I / Heterotic dual pair on T^4/Z_2.Comment: Minor corrections and clarifications. Added reference

On the origin of the zero-resistance anomaly in heavy fermion superconducting Ir: a clue from magnetic field and Rh-doping studies

We present the results of the specific heat and AC magnetic susceptibility measurements of $CeIr_{1-x}Rh_xIn_5$ for x from 0 to 0.5. As x is increased from 0 both quantities reflect the competition between two effects. The first is a suppression of superconductivity below the bulk transition temperature of T$_c = 0.4$ K, which is due to the pair breaking effect of Rh impurities. The second is an increase in the volume fraction of the superconducting regions above T$_c$, which we attribute to defect-induced strain. Analysis of the H-T phase diagram for CeIrIn$_5$obtained from the bulk probes and resistance measurements points to the filamentary origin of the inhomogeneous superconductivity at T$_\rho \approx 1.2$ K, where the resistance drops to zero. The identical anisotropies in the magnetic field dependence of the specific heat and the resistance anomalies in CeIrIn$_5$ indicate that the filamentary superconductivity is intrinsic, involving electrons from the part of the Fermi surface responsible for bulk superconductivity.Comment: 4 page