221 research outputs found
Paleomagnetism of a lateritic paleoweathering horizon and overlying Paleoproterozoic red beds from South Africa: Implications for the Kaapvaal apparent polar wander path and a confirmation of atmospheric oxygen enrichment
The Olifantshoek Group in southern Africa contains Paleoproterozoic red beds that are exceptionally well preserved, lying unconformably atop a regionally extensive lateritic paleoweathering profile. We studied the basal unit of this succession, known as the Gamagara or Mapedi Formation, and the lateritized substrate (so-called âDrakensteinâ or âWolhaarkopâ paleosol) on which it developed. Two ancient magnetic components are observed. One (INT), usually with a distributed unblocking spectrum between 300° and 600°C but occasionally persisting to >675°C, is directed shallowly southward or northward. A mesoscale fold test at South Sishen Mine indicates that this component was acquired during deformation; similarity of the direction to previous results suggests that it was acquired at âź1240 Ma, during early Namaqua orogenesis. Combining our INT results with existing data from the Namaqua eastern zone (NEZ), we calculate the NEZ pole at (44.9°N, 021.5°E, K = 23.2, A_95 = 12.8°, Q = 5). The most stable component from our data set (HIG), always persisting as a nonzero endpoint to demagnetization at >665°â680°C, is observed in 32 samples from South Sishen and Beeshoek Mines. Directed moderately east-downward (Sishen) or west-upward (Beeshoek), this component predates the mesoscale fold at Sishen. More importantly, a conglomerate test at Beeshoek indicates that HIG was acquired prior to Paleoproterozoic deposition of the Gamagara/Mapedi Formation. The concordance between directions from the paleoweathering zone and immediately overlying red beds indicates that HIG is a primary magnetic remanence for the basal Gamagara/Mapedi (BGM) Formation. The dual-polarity BGM paleomagnetic pole (02.2°N, 081.9°E, dp = 7.2°, dm = 11.5°, Q = 6) lies neatly between previous Kaapvaal poles with ages of âź2220 (Ongeluk lavas) and 2060 Ma (Bushveld complex). Our data thus support recent correlations of the Gamagara/Mapedi Formation with pre-Bushveld sediments of the Pretoria Group. A pre-Bushveld age for BGM is also consistent with its substantial distance from a new, albeit less reliable, paleomagnetic pole from the âź1930-Ma Hartley lavas, higher within the Olifantshoek succession (12.5°N, 332.8°E, K = 18.6, A95 = 16.0°, Q = 3). Our conglomerate test at Beeshoek confirms previous allegations that the intense hematitization observed in the Drakenstein-Wolhaarkop paleosol occurred during Paleoproterozoic weathering under a highly oxygenated atmosphere
Assessment of potential anti-cancer stem cell activity of marine algal compounds using an in vitro mammosphere assay
Background: The cancer stem cell (CSC) theory proposes that tumours arise from and are sustained by a subpopulation of cells with both cancer and stem cell properties. One of the key hallmarks of CSCs is the ability to grow anchorage-independently under serum-free culture conditions resulting in the formation of tumourspheres. It has further been reported that these cells are resistant to traditional chemotherapeutic agents.
Methods: In this study, the tumoursphere assay was validated in MCF-7 cells and used to screen novel marine algal compounds for potential anti-cancer stem cell (CSC) activity in vitro.
Results: MCF-7 breast cancer cells were observed to generate tumourspheres or mammospheres after 3-5 days growth in anchorage-independent conditions and an apparent enrichment in potential CSCs was observed by an increase in the proportion of CD44high/CD24low marker-bearing cells and Oct4 expression compared to those in the bulk population grown in regular adherent conditions. Using this assay, a set of algal metabolites was screened for the ability to inhibit mammosphere development as a measure of potential anti-CSC activity. We report that the polyhalogenated monoterpene stereoisomers RU017 and RU018 isolated from the red alga Plocamium cornutum, both of which displayed no cytotoxicity against either adherent MCF-7 breast cancer or MCF-12A non-transformed breast epithelial cells, were able to prevent MCF-7 mammosphere formation in vitro. On the other hand, neither the brown algal carotenoid fucoxanthin nor the chemotherapeutic paclitaxel, both of which were toxic to adherent MCF-7 and MCF-12A cells, were able to inhibit mammosphere formation. In fact, pre-treatment with paclitaxel appeared to enhance mammosphere formation and development, a finding which is consistent with the reported resistance of CSCs to traditional chemotherapeutic agents.
Conclusion: Due to the proposed clinical significance of CSC in terms of tumour initiation and metastasis, the identification of agents able to inhibit this subpopulation has clinical significance
Management for improved water use efficiency in the dry areas of Africa and West Asia: proceedings of a Workshop, Ankara, Turkey, 22-26 Apr 2002
This is a report of the 2002 workshop of the Optimizing Soil Water Use (OSWU) Consortium,
held in Ankara, Turkey. It describes OSWU research in West Asia (Jordan, Syria, Turkey), North
Africa (Morocco), Southern Africa (South Africa, Zimbabwe), and West Africa (Burkina Faso,
Niger). The consortium aims at developing and disseminating effective and practical solutions for
resource-poor farmers, being aware of the uncertainties of applying classical principles of soil-crop-
water relations in arid and semi-arid environments.
Reports from Morocco, Turkey, Jordan and South Africa confirm the effectiveness of some existing
technologies, including the use of mulches to reduce soil evaporation or runoff, sometimes
combined with use of soil fertility inputs to improve water use efficiency. Other papers describe a
new quality indicator to assess land degradation, the use of new decision support tools, and
modeling techniques to improve research efficiency and increase the effectiveness of farmer
participatory research. ICARDA and ICRISAT report on new developments within the
international research centers that are now ready for testing by NARS partners in their
environments.
Proposals for new work were presented and approved, with the emphasis on better transfer of
methods to improve soil water use, and evaluating the impact of past research projects. In
recognition of the current turbulent times, and the unlikelihood of increased resources for
agricultural research in dry areas, OSWU partners developed a strategic plan to achieve greater
impact; this plan is outlined
Depth-dependent δ13 C trends in platform and slope settings of the Campbellrand-Malmani carbonate platform and possible implications for Early Earth oxygenation
Highlights
⢠Carbon cycle of Neoarchean carbonate platform and potential oxygen oasis.
⢠Carbon isotopes reveal a shift to aerobic biosphere and increasing oxidation state.
⢠Rare earth element patterns reveal decrease in open ocean water influx.
⢠Rimmed margin architecture was crucial for evolution of aerobic ecosystems.
Abstract
The evolution of oxygenic photosynthesis is widely seen as the major biological factor for the profound shift from reducing to slightly oxidizing conditions in Earthâs atmosphere during the Archean-Proterozoic transition period. The delay from the first biogenic production of oxygen and the permanent oxidation of Earthâs atmosphere during the early Paleoproteorozoic Great Oxidation Event (GOE) indicates that significant environmental modifications were necessary for an effective accumulation of metabolically produced oxygen. Here we report a distinct temporal shift to heavier carbon isotope signatures in lagoonal and intertidal carbonates (δ13Ccarb from -1.6 to +0.2 â°, relative to VPDB) and organic matter (δ13Corg from about -40 to -25 â°, relative to VPDB) from the 2.58â2.50 Gy old shallowâmarine Campbellrand-Malmani carbonate platform (South Africa). This indicates an increase in the burial rate of organic matter caused by enhanced primary production as well as a change from an anaerobic to an aerobic ecosystem. Trace element data indicate limited influx of reducing species from deep open ocean water into the platform and an increased supply of nutrients from the continent, both supporting primary production and an increasing oxidation state of the platform interior. These restricted conditions allowed that the dissolved inorganic carbon (DIC) pool in the platform interior developed differently than the open ocean. This is supported by coeval carbonates from the marginal slope setting, which had a higher interaction with open ocean water and do not record a comparable shift in δ13Ccarb throughout the sequence. We propose that the emergence of stable shallow-water carbonate platforms in the Neoarchean provided ideal conditions for the evolution of early aerobic ecosystems, which finally led to the full oxidation of Earthâs atmosphere during the GOE
Carbon balance of a grazed savanna grassland ecosystem in South Africa
Tropical savannas and grasslands are estimated to contribute significantly to the total primary production of all terrestrial vegetation. Large parts of African savannas and grasslands are used for agriculture and cattle grazing, but the carbon flux data available from these areas are limited. This study explores carbon dioxide fluxes measured with the eddy covariance method for 3 years at a grazed savanna grassland in Welgegund, South Africa. The tree cover around the measurement site, grazed by cattle and sheep, was around 15 %. The night-time respiration was not significantly dependent on either soil moisture or soil temperature on a weekly temporal scale, whereas on an annual timescale higher respiration rates were observed when soil temperatures were higher. The carbon dioxide balances of the years 2010-2011, 2011-2012 and 2012-2013 were 85 +/- 16, 67 +/- 20 and 139 +/- 13 gCm(-2) yr(-1), respectively. The yearly variation was largely determined by the changes in the early wet season fluxes (September to November) and in the mid-growing season fluxes (December to January). Early rainfall enhanced the respiratory capacity of the ecosystem throughout the year, whereas during the mid-growing season high rainfall resulted in high carbon uptake.Peer reviewe
Ambient aromatic hydrocarbon measurements at Welgegund, South Africa
Aromatic hydrocarbons are associated with direct adverse human health
effects and can have negative impacts on ecosystems due to their toxicity,
as well as indirect negative effects through the formation of tropospheric
ozone and secondary organic aerosol, which affect human health, crop
production and regional climate. Measurements of aromatic hydrocarbons were
conducted at the Welgegund measurement station (South Africa), which is
considered to be a regionally representative background site. However, the
site is occasionally impacted by plumes from major anthropogenic source
regions in the interior of South Africa, which include the western Bushveld
Igneous Complex (e.g. platinum, base metal and ferrochrome smelters), the
eastern Bushveld Igneous Complex (platinum and ferrochrome smelters), the
JohannesburgâPretoria metropolitan conurbation (> 10 million
people), the Vaal Triangle (e.g. petrochemical and pyrometallurgical
industries), the Mpumalanga Highveld (e.g. coal-fired power plants and
petrochemical industry) and also a region of anticyclonic recirculation of
air mass over the interior of South Africa. The aromatic hydrocarbon
measurements were conducted with an automated sampler on Tenax-TA and
Carbopack-B adsorbent tubes with heated inlet for 1 year. Samples were
collected twice a week for 2 h during daytime and 2 h during
night-time. A thermal desorption unit, connected to a gas chromatograph and
a mass selective detector was used for sample preparation and analysis.
Results indicated that the monthly median (mean) total aromatic hydrocarbon
concentrations ranged between 0.01 (0.011) and 3.1 (3.2) ppb. Benzene levels
did not exceed the local air quality standard limit, i.e. annual mean of
1.6 ppb. Toluene was the most abundant compound, with an annual median
(mean) concentration of 0.63 (0.89) ppb. No statistically significant
differences in the concentrations measured during daytime and night-time
were found, and no distinct seasonal patterns were observed. Air mass back
trajectory analysis indicated that the lack of seasonal cycles could be
attributed to patterns determining the origin of the air masses sampled.
Aromatic hydrocarbon concentrations were in general significantly higher in
air masses that passed over anthropogenically impacted regions.
Inter-compound correlations and ratios gave some indications of the possible
sources of the different aromatic hydrocarbons in the source regions
defined in the paper. The highest contribution of aromatic hydrocarbon
concentrations to ozone formation potential was also observed in plumes
passing over anthropogenically impacted regions
Synergy between EngE, XynA and ManA from Clostridium cellulovorans on corn stalk, grass and pineapple pulp substrates
The synergistic interaction between various hemi/cellulolytic enzymes has become more important in order to achieve effective and optimal degradation of complex lignocellulose substrates for biofuel production. This study investigated the synergistic effect of three enzymes endoglucanase (EngE), mannanase (ManA) and xylanase (XynA) on the degradation of corn stalk, grass, and pineapple fruit pulp and determined the optimal degree of synergy between combinations of these enzymes. It was established that EngE was essential for degradation of all of the substrates, while the hemicellulases were able to contribute in a synergistic fashion to increase the activity on these substrates. Maximum specific activity and degree of synergy on the corn stalk and grass was found with EngE:XynA in a ratio of 75:25%, with a specific activity of 41.1Â U/mg protein and a degree of synergy of 6.3 for corn stalk, and 44.1Â U/mg protein and 3.4 for grass, respectively. The pineapple fruit pulp was optimally digested using a ManA:EngE combination in a 50:50% ratio; the specific activity and degree of synergy achieved were 52.4Â U/mg protein and 2.7, respectively. This study highlights the importance of hemicellulases for the synergistic degradation of complex lignocellulose. The inclusion of a mannanase in an enzyme consortium for biomass degradation should be examined further as this study suggests that it may play an important, although mostly overlooked, role in the synergistic saccharification of lignocellulose
Evidence for oxygenic photosynthesis half a billion years before the Great Oxidation Event
The early Earth was characterized by the absence of oxygen in the oceanâatmosphere system, in contrast to the well-oxygenated conditions that prevail today. Atmospheric concentrations first rose to appreciable levels during the Great Oxidation Event, roughly 2.5â2.3 Gyr ago. The evolution of oxygenic photosynthesis is generally accepted to have been the ultimate cause of this rise, but it has proved difficult to constrain the timing of this evolutionary innovation. The oxidation of manganese in the water column requires substantial free oxygen concentrations, and thus any indication that Mn oxides were present in ancient environments would imply that oxygenic photosynthesis was ongoing. Mn oxides are not commonly preserved in ancient rocks, but there is a large fractionation of molybdenum isotopes associated with the sorption of Mo onto the Mn oxides that would be retained. Here we report Mo isotopes from rocks of the Sinqeni Formation, Pongola Supergroup, South Africa. These rocks formed no less than 2.95 Gyr ago in a nearshore setting. The Mo isotopic signature is consistent with interaction with Mn oxides. We therefore infer that oxygen produced through oxygenic photosynthesis began to accumulate in shallow marine settings at least half a billion years before the accumulation of significant levels of atmospheric oxygen
Suggestions for Shaping Tinnitus Service Provision in Western Europe: Lessons from the COVIDâ19 Pandemic
Background:
Tinnitus severity has been exacerbated due to the COVIDâ19 pandemic and those with tinnitus require additional support. Such support should be informed by patient preferences and needs. The objective of this study was to gather information from individuals with tinnitus living in Europe to inform stakeholders of the (i) support they needed in relation to changes associated with the COVIDâ19 pandemic, and (ii) suggestions regarding tinnitus care for the future.
Methods:
A crossâsectional mixed methods study design was used using closed and openâended questions via an online survey. Data was gathered from 710 adults experiencing tinnitus in Western Europe, with the majority living in The Netherlands, Belgium, and Sweden. Data were analysed using qualitative content analysis and descriptive statistics.
Results:
Those with tinnitus indicated the following support needs during the pandemic (a) support for tinnitus, (b) support for hearingârelated difficulties, (c) social support, and (d) pandemicârelated support. Five directions for future tinnitus care were provided, namely (a) need for understanding professional support and access to multidisciplinary experts, (b) greater range of therapies and resources, (c) access to more information about tinnitus, (d) prioritizing tinnitus research, and (e) more support for hearing protection and hearing loss prevention.
Conclusions:
The findings point to the need for accessible (remote), patientâcentred, suitable and evidenceâbased tinnitus care. Insights from the current study can be used by various stakeholders including clinical practitioners and tinnitus support services to ensure those with tinnitus have access to the help and support required in order to reduce service provision insufficiencies
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