New benzilidenimine and amido derivatives: evaluation of antioxidant activity

The use of compounds with antioxidant activity is expected to be useful for the treatment of diseases were active oxygen species and free radicals play an important role [1,2]. Phenolic antioxidants react with free radicals by a process that usually involves the transfer of a hydrogen atom, resulting in a stable phenolic radical [1,2]. Aromatic amines and imines also contain active hydrogen atoms (NH) and by analogy it is expected that these atoms can be transferred to free radicals behaving the aromatic amines and imines as antioxidants.Numerous methods can be applied to evaluate the potential use of a new compound as an antioxidant and both chemical and electrochemical methods have been developed with this purpose [3,4]. The use of electrochemical methods, in particular voltammetric techniques, provides information regarding its reducing power. In this work benzilidenoimine and amido derivatives, presented in figure 1 and 2 respectively, were synthesized and their potential use as antioxidants was evaluated by cyclic voltammetry. The structure–activity relationships of the synthesised compounds were investigated in order to understand how the different functionalities affect their antioxidant activity.info:eu-repo/semantics/publishedVersio

Avaliação da qualidade do serviço educativo em contexto hospitalar: o exemplo do Centro Hospitalar Leiria-Pombal

No âmbito do protocolo celebrado entre a Escola Superior de Educação e Ciências Sociais – Instituto Politécnico de Leiria (ESECS-IPL) e o Centro Hospitalar Leiria – Pombal (CHLP), e ao abrigo do Grupo Projeto Creche1, foi desenvolvido um estudo exploratório com o objetivo de avaliar a qualidade dos serviços educativos em contexto hospitalar, recorrendo-se ao inquérito por questionário. O interesse sobre esta problemática surgiu da necessidade de melhorar o serviço educativo prestado a este nível no CHLP, assumindo-se a humanização hospitalar como princípio e meta a alcançar. Também a nível científico e social, o estudo ganha relevância na medida em que proporciona a reconstrução de conhecimento sobre a importância dos serviços educativos em contexto hospitalar, bem como, a reflexão sobre estas questões. O bem-estar da criança e a possibilidade de maximizar os seus potenciais, em situação de internamento, constituiu o foco do estudo. O presente relatório é constituído por uma breve revisão de literatura sobre a problemática em questão, seguida da apresentação do estudo empírico realizado

O ensino da filosofia através do debate

Com o presente relatório de investigação procura-se analisar o tema do ensino da Filosofia através do debate. O debate apresenta-se como um processo didáctico útil para o ensino da filosofia no contexto da aula do ensino secundário. A questão de fundo que percorre a temática enunciada é a seguinte: será o debate um bom treino para fazer afirmações consistentes? Pretendemos saber se o debate é um bom treino para fazer afirmações consistentes, se é uma boa “ferramenta” de ensino na disciplina de Filosofia. No sentido de juntar a componente lectiva ao trabalho de investigação, realizámos uma sessão de debate numa turma de Filosofia de 11.º ano. A orientação da mesma segue a perspectiva didáctica do debate enunciado por Oscar Brenifier

VMI_Vendor managed inventory : que vantagens competitivas?

Mestrado em Gestão de OperaçõesEnquadrando o tema na área de conhecimento respectiva, nomeadamente a Gestão da Cadeia de Abastecimento, pretende-se com este trabalho exploratório e compilatório procurar encontrar metodologias de sucesso nesta área de actuação, partindo e centralizando a sua análise em torno da mais recente solução VMI, ainda emergente mas já entendida por muitos como o corolário das mais dinâmicas práticas de gestão industrial, com vista à excelência da gestão do negócio assente na minimização de custos e esforços ligados à gestão da cadeia logística. ABSTRACT: Defining the theme on the knowledge area, namely the Supply Chain Management, this thesis, based on an explorer and compiling study and research, pretends to look for successful methodologies, focusing its analysis on the emerging solution VMI, still new but already understood and defended by many as the corollary of the most dynamic and lightning practices of industrial management, in order to achieve the excellence of the business based on the costs and efforts minimization concerning to the logistic chain performance

Single cell oil production by oleaginous yeasts grown in synthetic and waste-derived volatile fatty acids

Four yeast isolates from the species—Apiotrichum brassicae, Candida tropicalis, Metschnikowia pulcherrima, and Pichia kudriavzevii—previously selected by their oleaginous character and growth flexibility in different carbon sources, were tested for their capacity to convert volatile fatty acids into lipids, in the form of single cell oils. Growth, lipid yields, volatile fatty acids consumption, and long-chain fatty acid profiles were evaluated in media supplemented with seven different volatile fatty acids (acetic, butyric, propionic, isobutyric, valeric, isovaleric, and caproic), and also in a dark fermentation effluent filtrate. Yeasts A. brassicae and P. kudriavzevii attained lipid productivities of more than 40% (w/w), mainly composed of oleic (>40%), palmitic (20%), and stearic (20%) acids, both in synthetic media and in the waste-derived effluent filtrate. These isolates may be potential candidates for single cell oil production in larger scale applications by using alternative carbon sources, combining economic and environmental benefits.This work was supported by the European project "VOLATILE-Biowaste derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks" (Call H2020-NMBP-BIO-2016 Grant agreement No. 720777) and by the strategic programme UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P., by the ERDF through the COMPETE2020

Actividades experimentais nas escolas através de uma interacção com a universidade

A forma como a ciência é ensinada nas escolas afecta profundamente a percepção que os estudantes têm do mundo que os rodeia e consequentemente a escolha de uma futura carreira nas ciências. Por outro lado, a abertura da escola à comunidade envolvente, proporciona as condições para a sua participação activa na vida escolar, assim como a promoção do sucesso escolar, através da formação integral dos alunos, dotando-os das competências científicas, tecnológicas e sócio-culturais necessárias a sólidas opções futuras. A química como uma ciência de cariz experimental oferece aos estudantes uma melhor compreensão dos conceitos teóricos leccionados. Este trabalho descreve actividades experimentais realizadas no âmbito de um intercâmbio entre a Escola Secundária/3 de Barcelinhos e o Departamento de Química. Os projectos desenvolvidos foram “Museu das Ciências” na disciplina de Área Projecto do 12º ano de escolaridade. A actividade A Ciência na investigação criminal foi baseada nas oficinas apresentadas no projecto Sentidos da Ciência [1]. Estas actividades experimentais executadas em colaboração entre as escolas e a universidade contribuem para uma maior motivação dos alunos, uma formação científica mais sólida e um conhecimento mais integrado

Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source

Background: Over the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use of alternative and ecological carbon sources for yeast growth. Results: In the present study a high-throughput screening comprising 366 distinct yeast isolates was performed by applying an optimised protocol based on two approaches: (I) yeast cultivation on solid medium using acetic acid as carbon source, (II) neutral lipid estimation by fluorimetry using the lipophilic dye Nile red. Conclusions: Results showed that, with the proposed methodology, the oleaginous potential of yeasts with broad taxonomic diversity and variety of growth characteristics was discriminated. Furthermore, this work clearly demonstrated the association of the oleaginous yeast character to the strain level, contrarily to the species-level linkage, as usually stated.This work was supported by the European project “VOLATILE - Biowaste derived volatile fatty acid platform for biopolymers, bioactive compounds and chemical building blocks “(Call H2020-NMBP-BIO-2016 Grant agreement No. 720777) and by the strategic programme UID/BIA/04050/2013 (POCI-01- 0145-FEDER-007569) funded by national funds through the FCT I.P., by the ERDF through the COMPETE2020

Parkinsonian phenotype in Machado-Joseph disease (MJD/SCA3): a two-case report

Background: Machado-Joseph disease (MJD), or spinocerebellar ataxia type 3 (SCA3), is an autosomal dominant neurodegenerative disorder of late onset, which is caused by a CAG repeat expansion in the coding region of the ATXN3 gene. This disease presents clinical heterogeneity, which cannot be completely explained by the size of the repeat tract. MJD presents extrapyramidal motor signs, namely Parkinsonism, more frequently than the other subtypes of autosomal dominant cerebellar ataxias. Although Parkinsonism seems to segregate within MJD families, only a few MJD patients develop parkinsonian features and, therefore, the clinical and genetic aspects of these rare presentations remain poorly investigated. The main goal of this work was to describe two MJD patients displaying the parkinsonian triad (tremor, bradykinesia and rigidity), namely on what concerns genetic variation in Parkinson's disease (PD) associated loci (PARK2, LRRK2, PINK1, DJ-1, SNCA, MAPT, APOE, and mtDNA tRNAGln T4336C). Case presentation: Patient 1 is a 40 year-old female (onset at 30 years of age), initially with a pure parkinsonian phenotype (similar to the phenotype previously reported for her mother). Patient 2 is a 38 year-old male (onset at 33 years of age), presenting an ataxic phenotype with parkinsonian features (not seen either in other affected siblings or in his father). Both patients presented an expanded ATXN3 allele with 72 CAG repeats. No PD mutations were found in the analyzed loci. However, allelic variants previously associated with PD were observed in DJ-1 and APOE genes, for both patients. Conclusions: The present report adds clinical and genetic information on this particular and rare MJD presentation, and raises the hypothesis that DJ-1 and APOE polymorphisms may confer susceptibility to the parkinsonian phenotype in MJD