987 research outputs found
An alkylation route to carbo- and heteroaromatic amino acids
Amino acids carrying aromatic carbo- and heterocycles in the side chain, such as naphthyl-, biphenyl- and pyridylalanines, have been prepared by alkylation of a glycine enolate with a haloalkyl carbocycle or heterocycle, with enantiomeric excess up to 87% using the ephedrine amide protocol
Intergenerational Pathways From Reflective Functioning to Infant Attachment Through Parenting
The aim of this prospective study was to examine temporal pathways from mothersâ reflective functioning (RF) through parenting to infant attachment measured more than 16 months later. Participants were 88 motherâinfant dyads from demographically diverse backgrounds and included a group of mothers with histories of childhood maltreatment. RF was assessed using the RF rating of the Adult Attachment Interview before the birth of the baby. Parenting was assessed when the infants were 6 months old using the Maternal Sensitivity scale, as well as when they were 16 months using the Disconnected and Extremely Insensitive Parenting scale. Infant attachment was assessed when the infants were 16 months old using the Strange Situation. As hypothesised, the study findings showed that mothersâ mentalization regarding their own early attachment relationships was associated with later parenting and infant attachment. Negative parenting behaviours explained the link between mothersâ RF about their own attachment relationships and infant attachment disorganization. The findings suggest that mothersâ mentalization about their early attachment relationships has important implications in the transition to becoming parents themselves. Mentalization appears to be particularly important in helping mothers screen and inhibit negative parenting behaviours that would otherwise undermine infant attachment security and organization
Inverted repeat nucleotide sequences in the genomes of Marek disease virus and the herpesvirus of the turkey.
Mothersâ self-focused reflective functioning interacts with childhood experiences of rejection to predict romantic relationship quality and insensitive parenting
Parents exposed to rejection in their childhood could experience bonding disturbances in their current relationships. Reflective functioning (RF), the capacity to understand oneâs own and othersâ behavior through the lens of underlying mental states (cognitions, emotions), has been identified as a potential protective process. The aim of this longitudinal study was to examine whether RF moderates the effect of parentsâ experiences of rejection in childhood on later relationship functioning with partners and infants. Pregnant women with experiences of abuse and neglect were recruited and completed the Adult Attachment Interview, which was coded for RF and experiences of childhood rejection. During two followâup assessments, when their infants were 5 and 17 months old, the mothers in our sample who had partners reported on dyadic cohesion with these partners. Further, at 5 months postnatal, mothers completed interaction tasks with their infants, which were later assessed using observational measures (i.e., CAREâIndex). Results of mothers with partners (N = 93) indicated that RF moderated the relationship between dyadic cohesion with partners at 17 months only. Additionally, results with all mothers in the sample (N = 108) indicated that RF moderated the relationship between retrospectively reported experiences of rejection and controlling and unresponsive behaviors with infants. Adequateâtoâhigh RF was associated with lower unresponsiveness and higher relationship satisfaction in the context of rejection, while being associated with higher levels of control. These findings have important clinical implications, as RF is amenable to change and can therefore be more prominently implemented within various interventions
Ion track grafting: A way of producing low-cost and highly proton conductive membranes for fuel cell applications
International audienceKeywords: Ion track grafting Radiografting Swift heavy ions Proton conductivity Polymer electrolyte membrane Proton exchange membrane fuel cell a b s t r a c t Proton conductive individual channels through a poly(vinyl di-fluoride) PVDF matrix have been designed using the ion track grafting technique. The styrene molecules were radiografted and further sulfonated leading to sulfonated polystyrene (PSSA) domains within PVDF. The grafting process all along the cylindrical ion tracks creates after functionalisation privileged paths perpendicular to the membrane plane for proton conduction from the anode to the cathode when used in fuel cells. Such ion track grafted PVDF-g-PSSA membranes have low gas permeation properties against H 2 and O 2. A degree of grafting (Y w) of 140% was chosen to ensure a perfect coverage of PSSA onto PVDF-g-PSSA surface minimizing interfacial ohmic losses with the active layers of the Membrane Electrolyte Assembly (MEA). A three-day fuel cell test has been performed feeding the cell with pure H 2 and O 2 , at the anode and cathode side respectively. Temperature has been progressively increased from 50 to 80 âą C. Polarisation curves and Elec-trochemical Impedance Spectroscopy (EIS) at different current densities were used to evaluate the MEA performance. From these last measurements, it has been possible to determine the resistance of the MEA during the fuel cell tests and, thus the membrane conductivity. The proton conductivities of such membranes estimated during fuel cell tests range from 50 mS cm â1 to 80 mS cm â1 depending on the operating conditions. These values are close to that of perfluorosulfonated membrane such as Nafion Âź in similar conditions
In vivo time-lapse imaging of mitochondria in healthy and diseased peripheral myelin sheath
The myelin sheath that covers a large amount of neurons is critical for their homeostasis, and myelinating glia mitochondria have recently been shown to be essential for neuron survival. However morphological and physiological properties of these organelles remain elusive. Here we report a method to analyze mitochondrial dynamics and morphology in myelinating Schwann cells of living mice using viral transduction and time-lapse multiphoton microscopy. We describe the distribution, shape, size and dynamics of mitochondria in live cells. We also report mitochondrial alterations in Opa1(delTTAG) mutant mice cells at presymptomatic stages, suggesting that mitochondrial defects in myelin contribute to OPA1 related neuropathy and represent a biomarker for the disease
Impact of level of personality pathology on affective, behavioral, and thought problems in pregnant women during the Coronavirus Disease 2019 pandemic
Among at-risk groups for psychological distress in the context of the Coronavirus Disease 2019 (COVID-19) pandemic, pregnant women might be especially vulnerable. Identifying subgroups of pregnant women at high risk of poor adaptation might optimize clinical screening and intervention, which could, in turn, contribute to mitigating the potentially devastating effects of prenatal stress on mothers and fetus. Level of personality functioning may be a good indicator of who may be more vulnerable to distress in challenging periods like the COVID-19 pandemic, as adults with high levels of personality dysfunction may experience significant difficulties in mentalizing threatening situations. The aims of the present study are (a) to determine the impact of level of personality pathology on affective, behavioral, and thought problems in pregnant women during the COVID-19 pandemic; and (b) to test a model where mentalization of trauma mediates the impact of personality pathology on symptomatology. Data from 1,207 French-Canadian pregnant women recruited through social media during the COVID-19 pandemic were analyzed. Latent profile analysis, using the Criterion A elements of the alternative model for personality disorders (Identity, Self-Direction, Empathy, Intimacy) as latent indicators, yielded four profiles: Healthy, Mild Self-Impairment, Intimacy Impairment, and Personality Disorder. Profiles showed significant associations with diverse indicators of symptomatology. Mediation models showed both direct and indirect (through mentalization of trauma) significant associations between level of personality functioning and affective/behavioral/thought problems. Results have clinical implications on prophylactic measures for at-risk pregnant women, especially in challenging contexts such as the COVID-19 pandemic
Dinitrogen fixation and dissolved organic nitrogen fueled primary production and particulate export during the VAHINE mesocosm experiment (New Caledonia lagoon)
International audienceIn the oligotrophic ocean characterized by nitrate (NO â 3) depletion in surface waters, dinitrogen (N 2) fixation and dissolved organic nitrogen (DON) can represent significant nitrogen (N) sources for the ecosystem. In this study, we deployed large in situ mesocosms in New Caledonia in order to investigate (1) the contribution of N 2 fixation and DON use to primary production (PP) and particle export and (2) the fate of the freshly produced particulate organic N (PON), i.e., whether it is preferentially accumulated and recycled in the water column or exported out of the system. The mesocosms were fertilized with phosphate (PO 3â 4) in order to prevent phosphorus (P) limitation and promote N 2 fixation. The diazotrophic community was dominated by diatomâdiazotroph associations (DDAs) during the first part of the experiment for 10 days (P1) followed by the unicel-lular N 2-fixing cyanobacteria UCYN-C for the last 9 days (P2) of the experiment. N 2 fixation rates averaged 9.8 ± 4.0 and 27.7 ± 8.6 nmol L â1 d â1 during P1 and P2, respectively. NO â 3 concentrations ( 0.05) during P1 (9.0 ± 3.3 %) and P2 (12.6 ± 6.1 %). However, the e ratio that quantifies the efficiency of a system to export particulate organic carbon (POC export) compared to PP (e ratio = POC export / PP) was significantly higher (p 0.05) from the total amount of PON exported (0.10 ± 0.04 ”mol L â1), suggesting a rapid and probably direct export of the recently fixed N 2 by the DDAs. During P2, both PON concentrations and PON export increased in the mesocosms by a factor 1.5â2. Unlike in P1, this PON production was not totally explained by the new N provided by N 2 fixation. The use of DON, whose concentrations decreased significantly (p < 0.05) from 5.3 ± 0.5 ”mol L â1 to 4.4 ± 0.5 ”mol L â1 , appeared to be the missing N source. The DON consumption (⌠0.9 ”mol L â1) during P2 is higher Published by Copernicus Publications on behalf of the European Geosciences Union. 4100 H. Berthelot et al.: Dinitrogen fixation and dissolved organic nitrogen fueled primary production than the total amount of new N brought by N 2 fixation (⌠0.25 ”mol L â1) during the same period. These results suggest that while DDAs mainly rely on N 2 fixation for their N requirements, both N 2 fixation and DON can be significant N sources for primary production and particulate export following UCYN-C blooms in the New Caledonia lagoon and by extension in the N-limited oceans where similar events are likely to occur
Surface specific peptide immobilization on radiografted polymers as potential screening assays for antiangiogenic immunotherapy
International audienceAngiogenesis is a key process of cancer development and metastasis. It's inhibition is an important and promising strategy to block tumor growth and invasion. One of these approaches, based on antiangiogenic immunotherapy, is the recognition of a specific region of an angiogenic growth factor, called VEGF-A, by monoclonal antibodies. Thus, we aimed to design a novel assay to screen potential monoclonal antibodies directed against VEGF-A. In a first approach, we chose to perform covalent coupling of angiogenesis active cyclopeptides onto biocompatible thermoplastic transparent PVDF films and to fully characterize the chemical structure, the surface state and the biochemical properties of the synthesized devices. Electron beam radiation created radical sites on PVDF films without adding any toxic chemicals. These primary radicals and some induced peroxides were used as initiators for acrylic acid polymerization. Under our experimental conditions, surface grafting was favoured. Functionalization of PVDF-g-PAA films with peptides via a spacer arm was possible by performing two subsequent coupling reactions. EDC was used as coupling agent. Spacer arm saturation of the film surface was achieved for 25 mol% yield meaning that one spacer arm on four carboxylic acids were covalently bound. Peptide immobilization resulted in binding 10 times less leading to a final 3 mol% yield. Binding densities are governed by their individual space requirements. Each chemical step has been followed by FTIR in ATR mode, NMR using HR MAS technique and XPS. From XPS results, a layer of peptide covered PVDF-g-PAA film surface. The amounts of covalently immobilized peptide were determined using indirect UV spectroscopy on supernatant reaction solution. Yields were correlated with high resolution NMR results. The peptide/antibody recognition validated our system showing the conservation of peptide tridimensional structure with a positive response to specific antibodies. Because of the covalent protein linkage to PVDF films, a simple cleaning with immunoaffinity chromatography buffer allows the films to be reused
On the -supports of a holonomic -module
For a smooth variety over a perfect field of positive characteristic, the
sheaf of crystalline differential operators on (also called the sheaf
of -differential operators) is known to be an Azumaya algebra over
the cotangent space of the Frobenius twist of Thus to a
sheaf of modules over one can assign a closed subvariety of
called the -support, namely the support of seen as a sheaf
on We study here the family of -supports assigned to the
reductions modulo primes of a holonomic -module. We prove that
the Azumaya algebra of differential operators splits on the regular locus of
the -support and that the -support is a Lagrangian subvariety of the
cotangent space, for large enough. The latter was conjectured by
Kontsevich. Our approach also provides a new proof of the involutivity of the
singular support of a holonomic -module, by reduction modulo Comment: The article has been rewritten with much improved exposition as well
as some additional results, e.g. Corollary 6.3.1. This is the final version,
accepted for publication in Inventiones Mathematica
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