Architecture réseau pour véhicule de transport en commun communiquant

Avec la démocratisation des appareils mobiles, les transports en commun sont amenés à proposer de nouveaux services à leur usagers et notamment une connexion à Internet. Le véhicule de transport en commun agit alors comme un routeur mobile fournissant une connexion fiable à ses nœuds et doit pour cela être connecté en permanence à un point d'accès. Les zones de couverture étant limitées par les technologies utilisées et par les obstacles, des changements de réseaux sont alors nécessaires et provoquant différents évènements pouvant impacter les performances des protocoles de Transport : introduction de latences dues à la configuration des interfaces, modification des caractéristiques du chemin utilisé par la communication.. Dans cette thèse nous étudions cet impact en déterminant son origine puis nous proposons des solutions visant à le réduire de deux manières : en réduisant les latences introduites par le changement de réseau et en diminuant l'impact de la modification des caractéristiques du réseau.With the growing popularity of mobile devices, Public Transport will have to evolve and will have to introduce new services to customers, like an Internet connection onboard for example. The vehicle will act as a mobile router providing its nodes with a reliable connection and therefore will be forced to stay connected to an access point at any time. Since network coverage area is restricted depending on communication technology and environment, performing handovers is mandatory, leading to network events affecting Transport protocols efficiency: latencies are introduced by network configuration, network parameters are changed brutally. This thesis studies such impact by focusing on its source before giving solutions aiming at lowering handover impact. Two means were chosen: lowering latencies introduced by network configuration and avoiding network parameters modification impact on Transport protocols

Reduced in vitro susceptibility to artemisinin derivatives associated with multi-resistance in a traveller returning from South-East Asia

Decreased in vitro susceptibility to dihydroartemisinin (21.2 nM) and artesunate (16.3 nM) associated with decreased susceptibility or resistance to quinine (1131 nM), mefloquine (166 nM), lumefantrine (114 nM), pyronaridine (70.5 nM) and piperaquine (91.1 nM) is reported in a patient returning from South-East Asia after trekking along the Mekong from the south of Laos to the north of Thailand. Decreased in vitro susceptibility to artemisinin derivatives did not appear to be mediated by the number of copies of pfmdr1 or pfATPase6, pfcrt, pfmdr1 or pfmrp polymorphism. The high IC50 to mefloquine of this Asian isolate was not associated with pfmdr1 copy number. Pfnhe-1 microsatellite ms4760 showed a profile 7 (ms4760-7) with three repeats of DNNND and one repeat of DDDNHNDNHNN, which is associated with high quinine reduced susceptibility. The patient recovered in three days without relapse after treatment with the association of quinine and doxycycline. Decreased in vitro susceptibility to quinine and the delayed effect of doxycycline may both have contributed to the delayed parasite clearance time, D4 (0.5%) and D7 (0.004%). The in vitro data, with IC50 for dihydroartemisinin and artesunate were up to ten times those of the reference clone W2, which suggests that this isolate may be resistant to artemisinin derivatives, associated with a decreased susceptibility to quinine

Polymorphism of Plasmodium falciparum Na+/H+ exchanger is indicative of a low in vitro quinine susceptibility in isolates from Viet Nam

<p>Abstract</p> <p>Background</p> <p>The <it>Plasmodium falciparum </it>NA+/H+ exchanger (<it>pfnhe1</it>, gene PF13_0019) has recently been proposed to influence quinine (QN) susceptibility. However, its contribution to QN resistance seems to vary geographically depending on the genetic background of the parasites. Here, the role of this gene was investigated in <it>in vitro </it>QN susceptibility of isolates from Viet Nam.</p> <p>Method</p> <p>Ninety-eight isolates were obtained from three different regions of the Binh Phuoc and Dak Nong bordering Cambodia provinces during 2006-2008. Among these, 79 were identified as monoclonal infection and were genotyped at the microsatellite <it>pfnhe1 </it>ms4760 locus and <it>in vitro </it>QN sensitivity data were obtained for 51 isolates. Parasite growth was assessed in the field using the HRP2 immunodetection assay.</p> <p>Results</p> <p>Significant associations were found between polymorphisms at <it>pfnhe1 </it>microsatellite ms4760 and susceptibility to QN. Isolates with two or more DNNND exhibited much lower susceptibility to QN than those harbouring zero or one DNNND repeats (median IC₅₀of 682 nM <it>versus </it>median IC₅₀of 300 nM; <it>p </it>= 0.0146) while isolates with one NHNDNHNNDDD repeat presented significantly reduced QN susceptibility than those who had two (median IC₅₀of 704 nM <it>versus </it>median IC₅₀of 375 nM; p < 0.01). These QNR associated genotype features were mainly due to the over representation of profile 7 among isolates (76.5%). The majority of parasites had <it>pfcrt76T </it>and wild-type <it>pfmdr1 </it>(> 95%) thus preventing analysis of associations with these mutations. Interestingly, area with the highest median QN IC₅₀showed also the highest percentage of isolates carrying the <it>pfnhe1 </it>haplotype 7.</p> <p>Conclusions</p> <p>The haplotype 7 which is the typical Asian profile is likely well-adapted to high drug pressure in this area and may constitute a good genetic marker to evaluate the dissemination of QNR in this part of the world.</p

Quinine-Resistant Malaria in Traveler Returning from Senegal, 2007

We describe clinical and parasitologic features of in vivo and in vitro Plasmodium falciparum resistance to quinine in a nonimmune traveler who returned to France from Senegal in 2007 with severe imported malaria. Clinical quinine failure was associated with a 50% inhibitory concentration of 829 nmol/L. Increased vigilance is required during treatment follow-up

Genetic Structure of Plasmodium falciparum and Elimination of Malaria, Comoros Archipelago

Elimination interventions should be implemented simultaneously throughout the entire archipelago

Architecture Multi Domiciliée dans les Réseaux Mobiles : Diminution de l'Impact de la Mobilité sur les Protocoles de Transport

International audienceLes routeurs mobiles fournissent à leurs noeuds une connexion fiable avec une mobilité transparente. Les protocoles de Transport sont affectés par cette transparence car l'état du réseau d'accès change sans avertissement ren-dant l'évaluation du réseau difficile. Notre architecture propose d'informer les protocoles de Transport lorsqu'un changement de réseau est effectué afin de diminuer l'impact sur les communications. Cette solution est basée sur la défi-nition de plusieurs interfaces réseaux au niveau des noeuds du réseau mobile et l'utilisation du protocole de Transport multi domicilié SCTP

Giant Mimiviridae CsCl Purification Protocol

International audienceWhile different giant viruses' purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae family virions resulting from Acanthamoeaba castellanii infections. Viruses are harvested after cell lysis and purified through a high density CsCl gradient to optimize the isolation of the virus from the cell debris or other potential contaminants. Due to the large size of the virion capsids, reaching half a micrometer diameter, the quality of the process can be monitored by light microscopy. The resulting purified particles can then be used to perform new infections, DNA extraction, structural studies, sugar composition analyses, sub-compartment characterization or proteomic experiments

Architecture réseau pour véhicule de transport en commun communiquant

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Non-Intrusive Scheduling of TCP Flows

International audienceWe investigate how to build a non-intrusive scheduled TCP. For the flows of a given origin-destination pair, the objective is to schedule their TCP segments (according to some desired criteria) without modifying the network bandwidth-share used by these flows, which in turn ensures friendliness with respect to the rest of the network. We show that in order for a scheduling algorithm to be strictly non-intrusive, a sufficient and necessary condition is that the sender's and receiver's buffers are infinite. We then show that, under the additional condition that segments are neither lost or reordered, the number of active TCP flows can be minimized by size-based schedulers, and we propose a new scheduler FAIR, which guarantees that the transfer time of every TCP flow for the origin-destination pair is reduced. We develop SCHED_TCP, a user space implementation of our scheme in order to evaluate its performance on the Internet. Our experiments illustrate the non-intrusive property of SCHED_TCP, and also illustrate that the performance gain with SCHED_TCP can be considerable. Our scheme is scalable, and it could be incrementally deployed on the Internet improving the user experience on every origin-destination pair. The main application domain of our approach correspond to situations in which there are many TCP concurrent connections within the same origin-destination pair, this might happen as a consequence of HTTP 1.1, Web 2.0 applications using AJAX (Google Maps etc.), Split TCP, Parallel Sockets, and also with the use of ChromeBook's where the user accesses to all services through the same back-end server infrastructure