Fundraising Pitch: Little Italy

While researching Little Italy, it came to our attention that there are no existing homeless shelters, food banks, and most importantly; soup kitchens. Collectively, our goal is that we decided to each play the Leadership Role (George, 2018) to raise monetary funds and food donations at our planned fundraiser to contribute towards a soup kitchen to be implemented inside St. Agnes Church of Little Italy, Toronto. We believe it is important to include the homeless in the community and low-income members as they have the right to be fed and they each require healthy meals to survive. Our objectives are: receiving the church director’s approval to use their property and parking lot, ensure someone has or will obtain a Food Handler Certification to prepare and serve food to the public, and create/distribute flyers as well as advertise by word of mouth to raise awareness for the locals to participate and donate

Cost of Care Conversations: Perspectives from Rural Health Care Providers and Older Adult Patients

Older adults often struggle with health care costs. Cost of care (CoC) conversations are conversations between health care providers and patients to discuss direct and indirect costs associated with health care. These conversations have been found to increase patient compliance, but patients and health care providers often do not have these discussions. This article describes a project to provide Extension education to encourage CoC conversations for older adults and health care providers in rural counties in a southern state. To inform educational material development, 125 older adults and 51 health care providers completed surveys about their cost-related barriers to health care, attitudes and frequency of CoC conversations, and preferred educational methods. Older adults reported that they were most comfortable discussing health care costs with physicians and pharmacists but that health care providers rarely initiated these conversations. Health care providers indicated that they were comfortable talking about health care costs with patients and reported that they often initiate these conversations. Both older adults and health care providers indicated fact sheets as a top educational method. This project demonstrates how Extension educators can partner with health care providers to educate older adults about communicating cost-related challenges and needs

Knowledge and Perceptions of Latent Tuberculosis Infection among Chinese Immigrants in a Canadian Urban Centre

Background. Since most tuberculosis (TB) cases in immigrants to British Columbia (BC), Canada, develop from latent TB infection (LTBI), treating immigrants for LTBI can contribute to the eradication of TB. However, adherence to LTBI treatment is a challenge that is influenced by knowledge and perceptions. This research explores Chinese immigrants\u27 knowledge and perceptions towards LTBI in Greater Vancouver. Methods. This mixed methods study included a cross-sectional patient survey at BC\u27s Provincial TB clinics and two focus group discussions (FGDs) with Chinese immigrants. Data from FGDs were coded and analyzed in Simplified Chinese. Codes, themes, and selected quotes were then translated into English. Results. The survey identified a mean basic knowledge score: 40.0% (95% CI: 38.3%, 41.7%). FGDs confirmed that Chinese immigrants\u27 knowledge of LTBI was low, and they confused it with TB disease to the extent of experiencing LTBI associated stigma. Participants also expressed difficulties navigating the health system which impeded testing and treatment of LTBI. Online videos were the preferred format for receiving health information. Conclusion. We identified striking gaps in knowledge surrounding an LTBI diagnosis. Concerns of stigma may influence acceptance and adherence of LTBI treatment in Chinese immigrants. Integrating these findings into routine health care is recommended

Patient Transport in the Time of COVID-19: Using Health Care Failure Mode and Effect Analysis with Simulation to Test and Modify a Protocol

Introduction: In March 2020, in response to the COVID-19 pandemic, an interprofessional, interdisciplinary team at Maine Medical Center used Healthcare Failure Mode and Effect Analysis (HFMEA) and in situ simulation to rapidly identify and mitigate latent safety threats (LST) in patient transport protocols. Methods: Following HFMEA steps, stakeholders representing a variety of disciplines assembled to address transport of patients with COVID-19. A process map was created to describe the process. With hazard analysis using table-top simulation followed by in situ simulation, we identified, categorized, and scored LSTs. Mitigation strategies were identified during structured debriefing. Results: Fourteen LSTs were identified in the categories of infection prevention (4), care coordination (2), equipment (2), facilities (2), teams (2), clinical skills (1), and diagnosis and treatment (1). Of these, 10 had “critical” hazard scores. Mitigation solutions were tested with in situ simulation. Results were shared with leadership and led to changes in hospital-wide protocols. Discussion: The COVID-19 pandemic presented an urgent need to create or adapt protocols to keep patients and staff safe. Our team combined simulation with HFMEA methodology to improve the safety of protocols for transporting patients with COVID-19. Simulation enabled recreation of real-world experience that exposed LSTs more thoroughly than mental walkthroughs alone. Use of HFMEA methodology supported quantifying identified LSTs and proposing mitigation strategies, while in situ simulation facilitated testing many proposed strategies. Conclusions: HFMEA used with in situ simulation provides an effective method to efficiently and thoroughly probe a process for failure modes, providing practical mitigation strategies

Food composition at present: new challenges

Food composition data is important for stakeholders and users active in the areas of food, nutrition and health. Newchallenges related to the quality of food composition data reflect the dynamic changes in these areas while the emerging technologies create new opportunities. These challenges and the impact on food composition data for the Mediterranean region were reviewed during the NUTRIMAD 2018 congress of the Spanish Society for Community Nutrition. Data harmonization and standardization, data compilation and use, thesauri, food classification and description, and data exchange are some of the areas that require new approaches. Consistency in documentation, linking of information between datasets, food matching and capturing portion size information suggest the need for new automated tools. Research Infrastructures bring together key data and services. The delivery of sustainable networks and Research Infrastructures in food, nutrition and health will help to increase access to and effective use of food composition data. EuroFIR AISBL coordinates experts and national compilers and contributes to worldwide efforts aiming to produce and maintain high quality data and tools. A Mediterranean Network that shares high quality food composition data is vital for the development of ambitious common research and policy initiatives in support of the Mediterranean Diet.The authors gratefully acknowledge the contributions of the many members of EuroFIR and RICHFIELDS project (funded by the European Union’s Horizon 2020 research and innovation funding programme under grant agreement no. 654280) partners who contributed to the developments referred to in this articleinfo:eu-repo/semantics/publishedVersio

Generation of multipotent foregut stem cells from human pluripotent stem cells

Human pluripotent stem cells (hPSCs) could provide an infinite source of clinically relevant cells with potential applications in regenerative medicine. However, hPSC lines vary in their capacity to generate specialized cells, and the development of universal protocols for the production of tissue-specific cells remains a major challenge. Here, we have addressed this limitation for the endodermal lineage by developing a defined culture system to expand and differentiate human foregut stem cells (hFSCs) derived from hPSCs. hFSCs can self-renew while maintaining their capacity to differentiate into pancreatic and hepatic cells. Furthermore, near-homogenous populations of hFSCs can be obtained from hPSC lines which are normally refractory to endodermal differentiation. Therefore, hFSCs provide a unique approach to bypass variability between pluripotent lines in order to obtain a sustainable source of multipotent endoderm stem cells for basic studies and to produce a diversity of endodermal derivatives with a clinical value

An In Vitro Pipeline for Screening and Selection of Citrus-Associated Microbiota with Potential Anti-"Candidatus Liberibacter asiaticus" Properties.

Huanglongbing (HLB) is a destructive citrus disease that is lethal to all commercial citrus plants, making it the most serious citrus disease and one of the most serious plant diseases. Because of the severity of HLB and the paucity of effective control measures, we structured this study to encompass the entirety of the citrus microbiome and the chemistries associated with that microbial community. We describe the spatial niche diversity of bacteria and fungi associated with citrus roots, stems, and leaves using traditional microbial culturing integrated with culture-independent methods. Using the culturable sector of the citrus microbiome, we created a microbial repository using a high-throughput bulk culturing and microbial identification pipeline. We integrated an in vitro agar diffusion inhibition bioassay into our culturing pipeline that queried the repository for antimicrobial activity against Liberibacter crescens, a culturable surrogate for the nonculturable "Candidatus Liberibacter asiaticus" bacterium associated with HLB. We identified microbes with robust inhibitory activity against L. crescens that include the fungi Cladosporium cladosporioides and Epicoccum nigrum and bacterial species of Pantoea, Bacillus, and Curtobacterium Purified bioactive natural products with anti-"Ca. Liberibacter asiaticus" activity were identified from the fungus C. cladosporioides Bioassay-guided fractionation of an organic extract of C. cladosporioides yielded the natural products cladosporols A, C, and D as the active agents against L. crescens This work serves as a foundation for unraveling the complex chemistries associated with the citrus microbiome to begin to understand the functional roles of members of the microbiome, with the long-term goal of developing anti-"Ca Liberibacter asiaticus" bioinoculants that thrive in the citrus holosystem.IMPORTANCE Globally, citrus is threatened by huanglongbing (HLB), and the lack of effective control measures is a major concern of farmers, markets, and consumers. There is compelling evidence that plant health is a function of the activities of the plant's associated microbiome. Using Liberibacter crescens, a culturable surrogate for the unculturable HLB-associated bacterium "Candidatus Liberibacter asiaticus," we tested the hypothesis that members of the citrus microbiome produce potential anti-"Ca Liberibacter asiaticus" natural products with potential anti-"Ca Liberibacter asiaticus" activity. A subset of isolates obtained from the microbiome inhibited L. crescens growth in an agar diffusion inhibition assay. Further fractionation experiments linked the inhibitory activity of the fungus Cladosporium cladosporioides to the fungus-produced natural products cladosporols A, C, and D, demonstrating dose-dependent antagonism to L. crescens

Food Composition at Present: New Challenges

Food composition data is important for stakeholders and users active in the areas of food, nutrition and health. Newchallenges related to the quality of food composition data reflect the dynamic changes in these areas while the emerging technologies create new opportunities. These challenges and the impact on food composition data for the Mediterranean region were reviewed during the NUTRIMAD 2018 congress of the Spanish Society for Community Nutrition. Data harmonization and standardization, data compilation and use, thesauri, food classification and description, and data exchange are some of the areas that require new approaches. Consistency in documentation, linking of information between datasets, food matching and capturing portion size information suggest the need for new automated tools. Research Infrastructures bring together key data and services. The delivery of sustainable networks and Research Infrastructures in food, nutrition and health will help to increase access to and effective use of food composition data. EuroFIR AISBL coordinates experts and national compilers and contributes to worldwide efforts aiming to produce and maintain high quality data and tools. A Mediterranean Network that shares high quality food composition data is vital for the development of ambitious common research and policy initiatives in support of the Mediterranean Diet