Antagonistic effect of probiotic strains against two pathogens: Salmonella Typhimurium and E. coli O157:H7

El objetivo de este estudio fue probar la eficiencia de las cepas probióticas Lactobacillus acidophilus NCFM (L. acidophilus), Lactobacillus rhamnosus HN001 (L. rhmanosus) y Bifidobacterium animalis BI07 (B. animalis) para antagonizar los patógenos resistentes a antibióticos Salmonella enteritidis var Thyphimurium (Salmonella Thyphimurium) y Escherichia coli O157:H7 (E. coli O157:H7). Las tres cepas probióticas mostraron poseer un efecto antagónico contra las cepas patógenas Salmonella Thyphimurium y E. coli O157:H7 resistentes a antibióticos. En la prueba de “Well Diffusion”, L. acidophilus y L. rhamnosus presentaron reducciones estadísticamente semejantes entre ellas (P >0.05) de 37-41 mm para E. coli O157:H7 y de 32 – 41 mm para Salmonella Thyphimurium, mientras que B. animalis mostró reducciones menores (P0.05).Consejo Nacional de Ciencia y TecnologíaGastronómica La Buena MesaJabil Circuit GuadalajaraKurago BiotekUniversidad de Guadalajar

Monitoring of Trough Plasma Ganciclovir Levels and Peripheral Blood Cytomegalovirus (CMV)-Specific CD8+ T Cells To Predict CMV DNAemia Clearance in Preemptively Treated Allogeneic Stem Cell Transplant Recipients

It is uncertain whether monitoring plasma ganciclovir (GCV) levels is useful in predicting cytomegalovirus (CMV) DNAemia clearance in preemptively treated allogeneic stem cell transplant recipients. In this observational study, including 13 episodes of CMV DNAemia treated with intravenous (i.v.) GCV or oral valganciclovir, we showed that monitoring trough plasma GCV levels does not reliably predict response to therapy. Rather, immunological monitoring (pp65 and immediate-early [IE]-1-specific gamma interferon [IFN-γ]-producing CD8+ T cells) appeared to perform better for this purpose

Executive Summary of Survey Among Temporarily Homeless Families

Executive Summary of Survey Project Among Temporarily Homeless Families The agency we were assigned is a non-profit organization centered around providing housing and resources for houseless families in the Seattle region. The agency consists of five housing locations along with additional day centers. The housing locations are Burien, Northshore, The Regrade, Bellevue, and the Family Diversion Center. They all offer family housing services, healthcare, education, and employment. The goal of our project was to gather information from guests on the healthcare services that they have utilized. It was also used to gather requests for additional services at each site to improve quality of care. With the information collected, the health services at the agency will be improved and provide additional awareness to better meet the guests\u27 specific needs. Background The families within the agency are described as two-parent families (pregnant or with children), single parents of all genders (pregnant or with children), extended families, and families with pets. The guest population varies from people coming from Seattle to those who have arrived from across the world, resulting in a variety of languages spoken at each site. These factors also provide a truly diverse population, each culture being accompanied by their own specific needs. In 2021, 76% of the families served identified as Black, Indigenous, or People of Color (Mary’s Place, 2021). Out of all the guests at the agency, 40% of these families considered themselves African American or of African Descent. The agency commits to “create a workplace and service structure that advances equity, diversity, and inclusion as a priority” (p.7). In 2021, the agency served 592 families, had 138,644 overnight stays, served 415,932 meals, served 1,204 children and 592 families, and had 100+ women visit the day center on average each day. Across the multiple locations, the organization commits to address barriers such as social determinants of health, and empower parents to build family stability, secure housing, and prepare for employment. Some of the healthcare services offered are dental, optical, mental health services, pediatric healthcare, and substance use aid. The agency also offers housing, assistance, and support to families with children with life threatening illnesses within their Popsicle Program (Mary’s Place, n.d.). The organization’s Housing Services and Housing Specialist utilize a problem-solving approach along with light flexible funding to support their guests in finding permanent housing. Often, these families have a challenging time finding housing due to the Americans with Disabilities Act (ADA), size of the unit or family, language barriers, or other barriers. In 2021, the agency helped 549 families find permanent housing (Mary’s Place, 2021). A main priority among the guests and the organization is employment. The agency has partnered with 35+ different companies to provide a variety of employment options for the guests (Mary’s Place, n.d.). In addition, the Employer Engagement team offers training for specific careers, readiness skills, access to referrals, and preparations for long-term employment (Mary’s Place, n.d.). To accompany employment services, the organization also values education and encourages to maintain education for families by providing program resources for enhanced learning, language classes, GED classes, region-based school systems, and tutoring services (Mary’s Place, n.d.). These resources are provided for guests and for houseless families not currently sheltered (Mary’s Place, n.d.). Activities and Methods The foundation of our project was centered around the agency’s willingness to improve guest utilization of health services through survey conduction. We began our project by conducting a windshield survey of each shelter site. By doing this, we were able to get a sense of the community and understand the conditions that affect the health of the population. Moving forward, we collected data about the physical environment, social, economics, and services available (Rector, 2021). Using these observations, we developed a nursing diagnosis for the population. The nursing diagnosis is readiness for enhanced knowledge related to guest utilization of agency’s health services. The agency requested that we facilitate a previously designed survey, created by preceding SPU nursing students. Next, we researched the best method of conducting a high-quality survey in an ethical manner. An emphasis was placed on the importance of voluntary participation; therefore, we did not directly ask guests to fill out the surveys in order to avoid skewing data (Kelley et al., 2003). Our literature review revealed several different approaches to how to best conduct the surveys. We used incentives like baked goods and coloring pages for a greater participation outcome. We took into consideration the population of families at the agency and included an activity for children to do while parents took the surveys. We offered surveys to the guests to complete and made them aware of our intentions with the results (Kelley et al., 2003). We addressed the language barrier by using site-provided interpreter services. Our team conducted surveys at each agency location twice and input the data in an Excel document. We created graphs to display the data to the organization. Outcomes/Limitations We succeeded in collecting data from guests regarding the services they are utilizing and their additional needs from the organization. Visiting each site twice provided a greater opportunity to collect surveys resulting in more data. The results consisted of anonymous, voluntary responses which we compiled and analyzed. Since participation was voluntary, the results were limited based on how many guests decided to engage in the survey. We attempted to address this limitation by visiting each site twice to collect the most data possible for each location and offering incentives to encourage participation. Another limitation we came across was a language barrier between us and some of the guests. This was addressed by having nine translations of the survey available to expand participation. We also utilized interpreter phone services when needed to communicate with guests. Conclusion Overall, the project’s purpose was to conduct surveys at the agency locations and gather information about health services being used and current health concerns to improve care quality. We accomplished the organization’s goal by implementing the surveys amongst the guests. By doing this, we hope to have achieved the desired outcomes of increasing awareness of resources by guests, accessibility of resources, and guest satisfaction. With our project, the health services team can set annual goals for guests and the organization. We encourage the continuation of survey use within the agency. References Abdelazeem, B., Abbas, K.S., Amin, M.A., El-Shahat, N.A., Malik, B., Kalantary, A., & Eltobgy, M. (2022) The effectiveness of incentives for research participation: A systematic review and meta-analysis of randomized controlled trials. PLoS ONE, 17(4): e0267534. https://doi.org/10.1371/journal.pone.0267534 Kelley, K., Clark, B., Brown, V., & Sitzia, J. (2003). Good practice in the conduct and reporting of survey research. International Journal for Quality in Health Care, 15(3), 261-266. https://doi.org/10.1093/intqhc/mzg031 Orcher, L. T. (2007). Conducting a survey: Technique for a term project, (1st ed.). Routledge. https://doi.org/10.4324/9781315267937 Rector, C., & Stanley, M. J. (2021). Lippincott CoursePoint Enhanced for Rector\u27s Community and Public Health Nursing. Wolters Kluwer. vbk://9781975178284 Who we serve. (n.d.). Mary’s Place. Retrieved February 22, 2023, from https://www.marysplaceseattle.org/who-we-serve 2021 gratitude report. (2021). Mary’s Place. https://static1.squarespace.com/static/5b8989d231d4df1bccd7bcc7/t/6282aaeac941950e930ace53/1652730603879/2021+Gratitude+Report.pd

Transformar la formación inicial docente: una aproximación a los perfiles de prácticas de evaluación

Se presenta los hallazgos de la fase cualitativa de una investigación de corte mixto que pretende explicar la contribución de las prácticas de evaluación en la autorregulación académica de los estudiantes normalistas. En el estudio de caso múltiple y evaluativo, se aplicaron tres entrevistas a profundidad con técnica de recuerdo, a diez formadores de docentes sobre sus prácticas de evaluación, y la implicación de los estudiantes durante el desarrollo de un curso semestral para identificar sus conocimientos docentes (SHULMAN, 1987) así como analizar la construcción de nuevos conocimientos docentes en función de los perfiles de práctica de evaluación de los aprendizajes. Los docentes fueron seleccionados según el método de sujetos voluntarios y de cuotas, entre las cuales, debían hacer uso de alguna herramienta tecnológica en el proceso de evaluación. Los hallazgos permiten identificar tres patrones o perfiles de práctica de evaluación de los aprendizajes

Monitoring of plasma Torque teno virus, total Anelloviridae and Human Pegivirus 1 viral load for the prediction of infectious events and acute graft versus host disease in the allogeneic hematopoietic stem cell transplantation setting

Anelloviridae and Human Pegivirus 1 (HPgV-1) blood burden have been postulated to behave as surrogate markers for immunosuppression in transplant recipients. Here, we assessed the potential utility plasma Torque teno virus (TTV), total Anelloviridae (TAV), and HPgV-1 load monitoring for the identification of allogeneic hematopoietic stem cell transplantation recipients (allo-HSCT) at increased risk of infectious events or acute graft versus host disease (aGvHD). In this single-center, observational study, plasma TTV DNA, TAV DNA, and HPgV-1 RNA loads were monitored in 75 nonconsecutive allo-HSCT recipients (median age, 54 years). Monitoring was conducted before at baseline or by days +30, +60, +90, +120, and +180 after transplantation. Pneumonia due to different viruses or Pneumocystis jirovecii, BK polyomavirus-associated haemorrhagic cystitis (BKPyV-HC), and Cytomegalovirus DNAemia were the infectious events considered in the current study. Kinetics of plasma TTV, TAV DNA, and HPgV-1 RNA load was comparable, with though and peak levels measured by days +30 and day +90 (+120 for HPgV-1). Forty patients (53%) developed one or more infectious events during the first 180 days after allo-HSCT, whereas 29 patients (39%) had aGvHD (grade II-IV in 18). Neither, TTV, TAV, nor HPgV-1 loads were predictive of overall infection or CMV DNAemia. A TTV DNA load cut-off ≥4.40 log10 (pretransplant) and ≥4.58 log10 (baseline) copies/mL predicted the occurrence of BKPyV-HC (sensitivity ≥89%, negative predictive value, ≥96%). TTV DNA loads ≥3.38 log10 by day +30 anticipated the occurrence of aGvHD (sensitivity, 90%; negative predictive value, 97%). Pretransplant HPgV-1 loads were significantly lower (p = 0.03) in patients who had aGvHD than in those who did not. Monitoring of TTV DNA or HPgV-1 RNA plasma levels either before or early after transplantation may be ancillary to identify allo-HSCT recipients at increased risk of BKPyV-HC or aGvHD

Neikame, ser o no ser Wixárika

In Mexico the indigenous groups have been idealized as an example of worth and resistance of the different forms of conquest, nevertheless, the situation of social vulnerability that its members live, forces people to join a world that marginalizes them and in many cases, requires them to leave their own traditions, customs and ways of life, however, there are those who endure and adapt to the new environment through strategies in an effort to protect and transmit their cultural roots. The present work has the purpose of showing the duality experienced by the members of indigenous groups, when they choose to migrate to the city in search of better living conditions and the problems they face when forming their family life away from the community of origin, while facing to maintain their traditions in a globalized world, which is achieved through the life of Neikame, artist and activist promoter of the culture of the Wixárika people.En México los pueblos originarios han sido idealizados como ejemplo de valor y resistencia ante las diferentes formas de conquista, no obstante, la situación de vulnerabilidad social que viven sus integrantes, los obliga a incorporarse a un mundo que termina por marginarlos y en muchos casos, les exige abandonar tradiciones, costumbres y modos de vida, sin embargo, hay quienes resisten y se adaptan al nuevo entorno creando estrategias en afán de proteger y transmitir sus raíces culturales. El presente trabajo tiene la finalidad de mostrar la dualidad que experimentan los integrantes de pueblos originarios, cuando optan por migrar a la ciudad en busca de mejores condiciones de vida y de los problemas que enfrentan al formar su vida familiar lejos de la comunidad de origen, al tiempo que se esfuerzan por mantener sus tradiciones en medio de un mundo globalizado, lo cual, se logra a través de la vida de Neikame, artista y activista promotor de la cultura del pueblo Wixárika

ADAPTACIÓN DE UN SGC ISO 9001:2015 A LA ISO 21001:2018 EN PROGRAMAS DE POSGRADOS DE UN CENTRO CONACYT-CIATEQ, AC (ADAPTATION OF AN ISO 9001: 2015 TO ISO 21001:2018 IN POSTGRADUATE PROGRAMS OF A CONACYT CENTER-CIATEQ, AC)

Resumen El presente artículo describe la metodología para rediseñar los procesos actuales del Sistema de Gestión de Calidad (SGC) en la norma ISO 9001:2015 de un Centro CONACYT-CIATEQ en programas de posgrados, con el propósito de adaptarlos para el desarrollo e implementación de un Sistema de Gestión de Calidad Educativo (SGCE) que cumpla con la norma ISO 21001:2018, dicha metodología consta básicamente de 5 pasos los cuales son: Análisis comparativo de las normas en cuestión, diagnóstico inicial del SGC, planeación de actividades para la adaptación a la norma educativa, desarrollo e implementación del SGCE y finalmente evaluación, seguimiento y mejora del sistema de calidad; este reajuste en el sistema de calidad se realizó con la finalidad de contar con herramientas que nos permitan mejorar, aumentar la satisfacción y cumplir con las necesidades y expectativas de la comunidad estudiantil y partes interesadas educativas. Palabras Clave: ISO 9001:2018, ISO 21001:2018, SGC, SGCE. Abstract: This article describes the methodology to redesign the current processes of the Quality Management System (QMS) in the ISO 9001: 2015 standard of a CONACYT Center-CIATEQ in postgraduate programs, with the purpose of adapting them for the development and implementation of a System of Educational Quality Management (QMSE) that complies with the ISO 21001: 2018 standard, this methodology basically consists of 5 steps which are: Comparative analysis of the standards in question, initial diagnosis of the QMS, planning of activities for adaptation to the standard education, development and implementation of the QMSE and finally evaluation, monitoring and improvement of the quality system; This readjustment in the quality system was carried out in order to have tools that allow us to improve, increase satisfaction and meet the needs and expectations of the student community and educational stakeholders. Keywords: ISO 9001:2018, ISO 21001:2018, QMS, QMSE

Relevance of the detection of Streptococcus pneumoniae antigen in human urine in the diagnosis of lower respiratory tract infections

Fundamento y objetivo. Las técnicas de inmunocromatografía de membrana que detectan en orina el antígeno polisacárido C de neumococo, han experimentado un auge importante, aumentando las peticiones de antigenuria a los laboratorios de Microbiología Clínica. Se evaluó el impacto de la solicitud de esta prueba en el diagnóstico de las infecciones de las vías respiratorias bajas. Pacientes y método. Se realizaron 616 determinaciones de antigenuria mediante BinaxNOW® S. pneumoniae en otros tantos pacientes mayores de 14 años ingresados en el Hospital Universitario Virgen de las Nieves (Granada) entre noviembre de 2010 y marzo de 2011. Resultados. En el 91,1% de pacientes en los que se determinó la antigenuria, existió sintomatología respiratoria que justificó dicha solicitud. Sólo el 8,4% de las 616 antigenurias realizadas fueron positivas. S. pneumoniae fue aislado en el cultivo de la muestra respiratoria en 8 de estos 52 pacientes. En el 29,8% de los pacientes, el diagnóstico de infección de vías respiratorias bajas se basó en criterios clínicos, radiológicos y/o analíticos, ya que las antigenurias fueron negativas y no se realizó ninguna otra prueba microbiológica adicional. Conclusiones. Consideramos que esta técnica debe emplearse de forma complementaria, y nunca en detrimento de otras pruebas de diagnóstico microbiológico, especialmente en el paciente hospitalizado.Background and objective. Techniques membrane antigen immunochromatographic detecting in urine the pneumococcal polysaccharide C, have developed significantly, increasing requests for antigenuria to clinical microbiology laboratories. We evaluated the impact of the application of this test in the diagnosis of infections of lower respiratory tract. Patients and method. Six hundred and sixteen determinations were performed by antigenuria BinaxNOW® S. pneumoniae in as many patients over 14 years admitted to the Hospital Universitario Virgen de las Nieves (Granada) between November 2010 and March 2011. Results. In 91.1% of patients who were determined antigenuria the presence of respiratory symptoms justified the request. Only 8.4% of 616 antigenurias performed were positive. S. pneumoniae was isolated from the respiratory sample culture in 8 of these 52 patients. In 29.8% of patients the diagnosis of lower respiratory tract infection was based on clinical, radiological and/or analytical, as antigenurias were negative and did not involve any other additional microbiological test. Conclusions. We believe that this technique should be used in a complementary manner, and never to the detriment of other microbiological tests, especially in hospitalized patients

Case Report: Fibroglial Retinal Tissue in Contractile Morning Glory Disc Anomaly

The purpose of the present case is to describe a patient with tractional retinal detachment (RD) associated with contractile morning glory: a 17-year-old female, with a history of failed surgery for RD when she was 2 years old in her right eye (OD), nystagmus, and a limited visual acuity in the left eye (OS). The slit lamp examination showed phthisis bulbi in OD and the anterior segment was unremarkable in OS. Dilated fundus examination revealed a tractional RD in the posterior pole and peripapillary and preretinal fibrosis without evidence of intravitreal dispersion of retinal pigment epithelial cells. After surgery treatment, the RD resolved and the posterior segment showed a staphylomatous excavation around the optic disc anomaly with irregular contractions that folded the macular area. This were unrelated to light, breathing, or eye movements. Although morning glory disc anomaly is associated with RD, the early diagnosis can reverse structural changes. In this case, the rare association with contractile movements was found posterior to the pars plana vitrectomy after all the fibroglial epiretinal tissue was removed