O-MOORE-NICE!: new methodologies and algorithms for design and simulation of analog integrated circuits

No abstract

Blueberry Advisory Committee Extension Report

The 1986 edition of the Blueberry Advisory Committee Extension Reports was prepared for the Maine Blueberry Commission and the University of Maine Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Fertility Levels 2. Insect and Disease Fact Sheets 3. Development of Insect ID Information for Growers 4. Effect of Pruning Practices an Blueberry Insect Abundance 5. Control of Blueberry Maggot (Alternatives to Guthion) 6. Economic Thresholds and Control of Secondary Blueberry Pests 7. Chemical Control of Mummyberry Disease 8. Chemical Control of Botrytis Bloom Blight 9. Effects of Late Summer Fungicide Applications 10. Mowing vs. Burning - Comparisons of Disease Incidence 11. Long-term Effects of N and NPK Fertilizer on Plant Growth and Yield 12. Effect of Several Mulches on Frost Heaving, Soil Moisture, Soil Temperature and Rhizome Development 13. Interaction of Fertility and Pruning Practices on Soil 14. Effect of Block Freezing on Physical Characterization and Sugar Migration on Lowbush Blueberries 15. Demonstration of the Rota-Cone Vacuum Drying Process on Lowbush Blueberries 16. Production of a Blueberry Gelatin 17. Isolation and Characterization of Blueberry Pectin 18. The Effect of pH, Chemicals and Holding time-temperature on the color of Blueberry Puree 19. Effect of Hexazinone on Species Distribution in Lowbush Blueberry Fields 20. Evaluation of Postemergent Herbicides for Grass Control 21. Evaluation of Sulfonyl urea and lmidazoline compounds for Bunchberry Control 22. Use of Mechanical wiper with glyphosate or dicamba for control of dogbane 23. Hand-wiper Applications of Herbicides on Woody Weeds 24. Dogbane Control with 2% Glyphosate 25. Low Volume Solution of Asulam for Bracken Fern Control 26. Integrated Weed Management 27. 1986 Annual Report to the Maine Lowbush Blueberry Commissio

The coding microsatellite mutation profile of PMS2-deficient colorectal cancer

Lynch syndrome (LS) is caused by a pathogenic heterozygous germline variant in one of the DNA mismatch repair (MMR) genes: MLH1, MSH2, MSH6 or PMS2. LS-associated colorectal carcinomas (CRCs) are characterized by MMR deficiency and by accumulation of multiple insertions/deletions at coding microsatellites (cMS). MMR deficiency-induced variants at defined cMS loci have a driver function and promote tumorigenesis. Notably, PMS2 variant carriers face only a slightly increased risk of developing CRC. Here, we investigate whether this lower penetrance is also reflected by differences in molecular features and cMS variant patterns. Tumor DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue cores or sections (n = 90). Tumors originated from genetically proven germline pathogenic MMR variant carriers (including 14 PMS2-deficient tumors). The mutational spectrum was analyzed using fluorescently labeled primers specific for 18 cMS previously described as mutational targets in MMR-deficient tumors. Immune cell infiltration was analyzed by immunohistochemical detection of T-cells on FFPE tissue sections. The cMS spectrum of PMS2-deficient CRCs did not show any sig-nificant differences from MLH1/MSH2-deficient CRCs. PMS2-deficient tumors, however, displayed lower CD3-positive T-cell infiltration compared to other MMR-deficient cancers (28.00 vs. 55.00 per 0.1 mm(2), p = 0.0025). Our study demonstrates that the spectrum of potentially immunogenic cMS variants in CRCs from PMS2 gene variant carriers is similar to that observed in CRCs from other MMR gene variant carriers. Lower immune cell infiltration observed in PMS2-deficient CRCs could be the result of alternative mechanisms of immune evasion or immune cell exclusion, similar to those seen in MMR-proficient tumors.Hereditary cancer genetic

Blueberry Advisory Committee Research Report

The 1987 edition of the Blueberry Advisory Committee Research Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include: 1. Effect of Pruning Practices on Blueberry Insect Abundance 2. Economic Thresholds and Control of Secondary Blueberry Pests 3. Control of Blueberry Maggot 4. Evaluation of Fungicides for Control of Botrytis Blight on Lowbush Blueberry, 1987 5. Evaluation of Fungicides for Control of Mummy Berry on Lowbush Blueberry, 1987 6. Miscellaneous Activity: a) Fungicide Residue Analysis b) Blueberry fact sheet c) Berry contamination 7. Effects of Pruning Methods on Mummy Berry Disease Incidence, 1987 8. Effects of Pruning Methods on Powdery Mildew and Red Leaf Disease Incidence, 1987 9. Nutrition Survey 1987 10. Nutritional Responses of Seedlings 11. The Effect of Several Mulches on Frost Heaving, Soil Moisture, Soil Temperature and Rhizome Development 12. Time-temperature Effects on Sugar Migration and Physical Characterization in Lowbush Blueberries 13. Fabricated Blueberry Raisins Prepared from Puree using Alginate as the Gelling Agent 14. Blueberry Gelatin 15. Effect of Hexazinone (VELPAR) on Species Distribution in Lowbush Blueberry Fields 16. Evaluation of Postemergence Herbicides for Grass Control 17. Evaluation of Sulfonyl urea and Imidazoline compounds for Bunchberry Control 18. Use of Mechanical wiper with glyphosate (ROUNDUP) or dicamba for control of dogbane 19. Integrated Weed Management 20. Evaluation of Five Preemergence Herbicides for Control of Oat grass and Bunchgrass 21. Wiper Application of Dicamba for Woody Weed control 22. Hexazinone (VELPAR) and terbacil (SINBAR) combinations for weed control 23. Evaluation of Clopyralid and Lactofen for Bunchberry Control 24. Evaluation of Postemergence Applications of chlorimuron for Bunchberry Control 25. Hexazinone seedling study 26. Seedling Pruning Study 27. Effect of bracken fern on blueberry yield 28. Evaluation of two mechanical harvesters vs hand raking of lowbush blueberrie

NEAs: Phase Angle Dependence of Asteroid Class and Diameter from Observational Studies

We will discuss the results of a planned observation campaign of Near Earth Asteroids (NEAs), 1999 CU3, 2002 GM2, 2002 FG7, and 3691 Bede with instruments on the United Kingdom Infrared Telescope (UKIRT) from 15-Mar-2015 to 28-April 2015 UT. We will study the phase-angle dependence of the reflectance and thermal emission spectra. Recent publications reveal that the assignment of the asteroid class from visible and near-IR spectroscopy can change with phase angle for NEAs with silicate-bearing minerals on their surfaces (S-class asteroids) (Thomas et al. 2014, Icarus 228, 217; Sanchez et al. 2012 Icarus 220, 36). Only three of the larger NEAs have been measured at a dozen phase angles and the trends are not all the same, so there is not yet enough information to create a phase-angle correction. Also, the phase angle effect is not characterized well for the thermal emission including determination of the albedo and the thermal emission. The few NEAs were selected for our study amongst many possible targets based on being able to observe them through a wide range of phase angles, ranging from less than about 10 degrees to greater than 45 degrees over the constrained date range. The orbits of NEAs often generate short observing windows at phase angles higher than 45 deg (i.e., whizzing by Earth and/or close to dawn or dusk). Ultimately, lowering the uncertainty of the translation of asteroid class to meteorite analog and of albedo and size determinations are amongst our science goals. On a few specific nights, we plan to observe the 0.75-2.5 micron spectra with IRTF+SpeX for comparison with UKIRT data including 5-20 micron with UKIRT+UIST/Michelle to determine as best as possible the albedos. To ensure correct phasing of spectroscopic data, we augment with TRAPPIST-telescope light curves and R-band guider image data. Our observations will contribute to understanding single epoch mid-IR and near-IR measurements to obtain albedo, size and IR beaming parameters (the outcomes of thermal models) and asteroid spectral class

Persistence with Statins and Onset of Rheumatoid Arthritis: A Population-Based Cohort Study

In a retrospective cohort study, Gabriel Chodick and colleagues find a significant association between persistence with statin therapy and reduced risk of developing rheumatoid arthritis, but only a modest decrease in risk of osteoarthritis

Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography at X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead