Surface sticking and lateral diffusion of lipids in supported bilayers

The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the data can be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes

Interactions between vaccinia virus and sensitized macrophages in vitro

The action of peritoneal exudate cells (PEC) from normal and vaccinia virus infected mice on infectious vaccinia virus particles was investigatedin vitro. PEC from immune mice showed a significantly higher infectivity titre reduction (virus clearance, VC) than normal cells. This effect could be clearly attributed to the macrophage. Vaccinia virus multiplied in PEC from normal animals while there was no virus propagation in cells from immunized mice. The release of adsorbed or engulfed virus was reduced significantly in PEC from immunized animals. Anti-vaccinia-antibodies seem to activate normal macrophages to increased virus clearance. This stimulating effect was demonstrable only in the IgG fraction of the antiserum. The activity of macrophages from mice injected three times over a period of 14 days with vaccinia virus could be entirely blocked with anti-mouse-IgG, while PEC from mice injected one time six days previously were not inhibited

Wildfire may increase habitat quality for spring Chinook salmon in the Wenatchee River subbasin, WA, USA

Pacific Northwest salmonids are adapted to natural disturbance regimes that create dynamic habitat patterns over space and through time. However, human land use, particularly long-term fire suppression, has altered the intensity and frequency of wildfire in forested upland and riparian areas. To examine the potential impacts of wildfire on aquatic systems, we developed stream-reach-scale models of freshwater habitat for three life stages (adult, egg/fry, and juvenile) of spring Chinook salmon (Oncorhynchus tshawytscha) in the Wenatchee River subbasin, Washington. We used variables representing pre- and post-fire habitat conditions and employed novel techniques to capture changes in in-stream fine sediment, wood, and water temperature. Watershed-scale comparisons of high-quality habitat for each life stage of spring Chinook salmon habitat suggested that there are smaller quantities of high-quality juvenile overwinter habitat as compared to habitat for other life stages. We found that wildfire has the potential to increase quality of adult and overwintering juvenile habitat through increased delivery of wood, while decreasing the quality of egg and fry habitat due to the introduction of fine sediments. Model results showed the largest effect of fire on habitat quality associated with the juvenile life stage, resulting in increases in high-quality habitat in all watersheds. Due to the limited availability of pre-fire high-quality juvenile habitat, and increased habitat quality for this life stage post-fire, occurrence of characteristic wildfires would likely create a positive effect on spring Chinook salmon habitat in the Wenatchee River subbasin. We also compared pre- and post-fire model results of freshwater habitat for each life stage, and for the geometric mean of habitat quality across all life stages, using current compared to the historic distribution of spring Chinook salmon. We found that spring Chinook salmon are currently distributed in stream channels in which in-stream habitat for most life stages has a consistently positive response to fire. This compares to the historic distribution of spring Chinook, in which in-stream habitat exhibited a variable response to fire, including decreases in habitat quality overall or for specific life stages. This suggests that as the distribution of spring Chinook has decreased, they now occupy those areas with the most positive potential response to fire. Our work shows the potentially positive link between wildfire and aquatic habitat that supports forest managers in setting broader goals for fire management, perhaps leading to less fire suppression in some situations

Modeling potential masking of echolocating sperm whales exposed to continuous 1–2 kHz naval sonar

This study was sponsored by the U.S. Living Marine Resources program, Office of Naval Research (ONR) Grant Nos. N00014-18-1-2062 and N00014-20-1-2709, UK Defence Science and Technology Laboratory (DSTL), French Direction générale de l'armement (DGA), and the Netherlands Ministry of Defence.Modern active sonar systems can (almost) continuously transmit and receive sound, which can lead to more masking of important sounds for marine mammals than conventional pulsed sonar systems transmitting at a much lower duty cycle. This study investigated the potential of 1–2 kHz active sonar to mask echolocation-based foraging of sperm whales by modeling their echolocation detection process. Continuous masking for an echolocating sperm whale facing a sonar was predicted for sonar sound pressure levels of 160 dB re 1 μPa2, with intermittent masking at levels of 120 dB re 1 μPa2, but model predictions strongly depended on the animal orientation, harmonic content of the sonar, click source level, and target strength of the prey. The masking model predicted lower masking potential of buzz clicks compared to regular clicks, even though the energy source level is much lower. For buzz clicks, the lower source level is compensated for by the reduced two-way propagation loss to nearby prey during buzzes. These results help to predict what types of behavioral changes could indicate masking in the wild. Several key knowledge gaps related to masking potential of sonar in echolocating odontocetes were identified that require further investigation to assess the significance of masking.Publisher PDFPeer reviewe

Effects of summary knowledge of results in motor skills acquisition

The effects of Summary Knowledge of Results (KR) were tested, using 30 volunteers and a positioning task in which a tennis ball had to be transported in 30 trials, following a specific sequence and with a target time of 3000 msec. Ten minutes after the acquisition phase, the transfer test was performed with 10 trials of different sequences and target times. The retention test took place 24 hours later with 10 trials of the same sequence and target time as the acquisition phase. In the transfer and retention tests, KR was not provided. The volunteers were randomly divided into three groups: G5 (KR every five trials); G3 (KR every three trials) and G100 (KR every trial). The results showed that G3 had a smaller absolute error than G100. However, G3 and G5 had a smaller constant error than G100. In general, the effects of G3 and G5 on motor skill acquisition could be caused by the lower KR frequency, which was 33% and 20% respectively.El efecto del conocimiento de los resultados (KR) resumen ha sido probado por 30 voluntarios en tarea de posicionamiento que requiere el transporte de una pelota de tenis en una secuencia específica con el tiempo objetivo de 3000 ms. en 30 ensayos. Diez minutos después de la fase de adquisición se jugó el test de transferencia con 10 ensayos con diferente secuencia y tempo objetivo. La retención se realizó la prueba 24 horas más tarde con la misma secuencia y tiempo objetivo fase de adquisición. En los testes de transferencia y retención de la CR no fue suministrado. Los voluntarios fueron divididos aleatoriamente en tres grupos: G5 (CR después de 5 ensayos); G3 (CR después de 3 ensayos) y G100 (CR en todos los ensayos). El resultado mostró que G3 tuvieron menor error absoluto que G100. Sin embargo, G3 y G5 se han registrado menor error constante que G100. En general, los efectos de G3 y G5 en la adquisición de las habilidades motoras pueden ser causados por la disminución de frecuencias CR, que fueron 33% y 20 %, respectivamente.O efeito do conhecimento de resultados (CR) sumário foi testado por 30 voluntários em uma tarefa de posicionamento a qual exigia o transporte de uma bola de tênis em uma sequência específica com tempo alvo de 3000 mseg. durante 30 tentativas. Dez minutos após a fase de aquisição foi desempenhado o teste de transferência com 10 tentativas com sequência e tempo alvo diferente. O teste de retenção foi realizado 24 horas mais tarde com a mesma sequência e tempo alvo da fase de aquisição. Nos testes de transferência e retenção o CR não foi fornecido. Os voluntários foram aleatoriamente divididos em três grupos: G5 (CR depois de 5 tentativas); G3 (CR depois de 3 tentativas) e G100 (CR em todas tentativas). O resultado mostrou que G3 apresentou menor erro absoluto que G100. Entretanto, G3 e G5 registraram menor erro constante que G100. Em geral, os efeitos de G3 e G5 sobre a aquisição de habilidades motoras podem ser causados pelas frequências reduzidas de CR, que foram de 33% e 20%, respectivamente

Quantemol Electron Collisions (QEC): An Enhanced Expert System for Performing Electron Molecule Collision Calculations Using the R-Matrix Method

Collisions of low energy electrons with molecules are important for understanding many aspects of the environment and technologies. Understanding the processes that occur in these types of collisions can give insights into plasma etching processes, edge effects in fusion plasmas, radiation damage to biological tissues and more. A radical update of the previous expert system for computing observables relevant to these processes, Quantemol-N, is presented. The new Quantemol Electron Collision (QEC) expert system simplifyies the user experience, improving reliability and implements new features. The QEC graphical user interface (GUI) interfaces the Molpro quantum chemistry package for molecular target setups, and the sophisticated UKRmol+ codes to generate accurate and reliable cross-sections. These include elastic cross-sections, super elastic cross-sections between excited states, electron impact dissociation, scattering reaction rates, dissociative electron attachment, differential cross-sections, momentum transfer cross-sections, ionization cross sections, and high energy electron scattering cross-sections. With this new interface we will be implementing dissociative recombination estimations, vibrational excitations for neutrals and ions, and effective core potentials in the near future

How effectively do horizontal and vertical response strategies of long-finned pilot whales reduce sound exposure from naval sonar?

PJW was supported with studentships of The Netherlands Ministry of Defence (grant number 032.30370/01.02) and the VSB Foundation (grant number VSB.08/228-E) and Ren e Dekeling is acknowledged for making funding possible. The 3S project was supported by the US Office of Naval Research, The Netherlands Ministry of Defence, Royal Norwegian Navy and Norwegian Ministry of Defence, and by World Wildlife Fund Norway. PLT received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged.The behaviour of a marine mammal near a noise source can modulate the sound exposure it receives. We demonstrate that two long-finned pilot whales both surfaced in synchrony with consecutive arrivals of multiple sonar pulses. We then assess the effect of surfacing and other behavioural response strategies on the received cumulative sound exposure levels and maximum sound pressure levels (SPLs) by modelling realistic spatiotemporal interactions of a pilot whale with an approaching source. Under the propagation conditions of our model, some response strategies observed in the wild were effective in reducing received levels (e.g. movement perpendicular to the source's line of approach), but others were not (e.g. switching from deep to shallow diving; synchronous surfacing after maximum SPLs). Our study exemplifies how simulations of source-whale interactions guided by detailed observational data can improve our understanding about motivations behind behaviour responses observed in the wild (e.g., reducing sound exposure, prey movement).PostprintPeer reviewe

The Possibly Remnant Massive Outflow in G5.89-0.39: I - Observations and Initial MHD Simulations

We have obtained maps of the large scale outflow associated with the UCHII regionG5.89-0.39 in CO and 13CO (J=3-2), SiO (J=8-7,J=5-4), SO2 (13(2,12)-13(1,13)) and H13CO+(J=4-3). From these maps we have been able to determine the mass (3.3 M_sun), momentum (96 M_sun km/s), energy (3.5x10^{46} erg), mechanical luminosity (141 L_sun), and mass loss rate (~1x10^{-3}M_sun/yr) in the large scale outflow. The observationally derived parameters were used toguide 3D magnetohydrodynamic models of the jet entrained outflow. Through the combination of observations and simulations, we suggest that the large scale outflowmay be inclined by approximately 45 deg to the line of sight, and that the jet entraining the observed molecular outflow may have been active for as little as 1000 years, half the kinematic age of the outflow.Comment: 27 Pages, 9 Figures, Accepted to ApJ. For full (High) resolution figures, please see http://www.ism.ucalgary.ca/~pamela/papers/index.htm

Harbour porpoise movement strategy affects cumulative number of animals acoustically exposed to underwater explosions

Anthropogenic sound in the marine environment can have negative consequences for marine fauna. Since most sound sources are intermittent or continuous, estimating how many individuals are exposed over time remains challenging, as this depends on the animals' mobility. Here we explored how animal movement influences how many, and how often, animals are impacted by sound. In a dedicated study, we estimated how different movement strategies affect the number of individual harbour porpoises Phocoena phocoena receiving temporary or permanent hearing loss due to underwater detonations of recovered explosives (mostly WWII aerial bombs). Geo-statistical distribution models were fitted to data from 4 marine mammal aerial surveys and used to simulate the distribution and movement of porpoises. Based on derived dose-response thresholds for temporary (TTS) or permanent threshold shifts (PTS), we estimated the number of animals affected in a single year. When individuals were free-roaming, an estimated 1200 and 24 000 unique individuals would suffer PTS and TTS, respectively. This equates to respectively 0.50 and 10% of the estimated North Sea population. In contrast, when porpoises remained in a local area, fewer animals would receive PTS and TTS (1100 [0.47%] and 15 000 [6.5%], respectively), but more individuals would be subjected to repeated exposures. Because most anthropogenic sound-producing activities operate continuously or intermittently, snapshot distribution estimates alone tend to underestimate the number of individuals exposed, particularly for mobile species. Hence, an understanding of animal movement is needed to estimate the impact of underwater sound or other human disturbance. © The authors 2016