253 research outputs found
Effet du chrome niacinate sur la tolérance au glucose chez le rat wistar
Le chrome est un oligoĂ©lĂ©ment essentiel Ă lâhomĂ©ostasie du mĂ©tabolisme glucidique qui pourrait Ă©galement ĂȘtre impliquĂ© dans lâĂ©tiologie de lâathĂ©rosclĂ©rose. Trois lots de rats Wistar males (n= 30, ĂągĂ© de 2 mois) traitĂ©s quotidiennement et pendant 28 jours. Les ContrĂŽles ont reçu du sĂ©rum physiologique (1”L/g/j, ip), groupe II a reçu la dexamĂ©thasone (DEX : 0,2 mg/kg/j, ip) et les rats du groupe III ont reçu le mĂȘme rĂ©gime que le groupe II avec une supplĂ©mentation en CrN Ă partir du 7 Ăšme jour de lâexpĂ©rimentation (CrN : 30 mg/kg/j, PO). A la fin de lâexpĂ©rimentation, les rats sont sacrifiĂ©s et les paramĂštres biochimiques sont dosĂ©s. Les rĂ©sultats montrent une augmentation du poids corporel des rats tĂ©moins par apport aux groupes traitĂ©s par la DEX. La supplĂ©mentation en CrN nâa pas corrigĂ© lâĂ©lĂ©vation de la glycĂ©mie et de lâinsulinĂ©mie engendrĂ© par la DEX, de mĂȘme lâajout du CrN nâinflue pas sur la variation des taux de la triglycĂ©ridĂ©mie et de la cholestĂ©rolĂ©mie entrainĂ©s par la DEX. Il est notamment observĂ© que lâaddition du CrN nâa pas dâeffet notable sur la fonction hĂ©patique et la fonction rĂ©nale.Mots-clĂ©s : chrome, diabĂšte, glycĂ©mie, dexamĂ©thasone.Effect of chromium niacinate on glucose tolerance at wistar rat Chromium is an essential trace element in the homeostasis of glucose metabolism could also be involved in the etiology of atherosclerosis. Three groups of male Wistar rats (n= 30, age 2 months) treated daily for 28 days. Controls received saline (1”l/g/day, ip), group II received dexamethasone (DEX: 0, 2 mg/kg/day, ip) and the rats of group III received the same treatment as the group II with supplementation CrN from the 7 day of the experiment (CrN: 30 mg/kg/day, PO). At the end of the experiment, the rats were sacrificed and biochemical parameters were measured. The results show an increase in the body weight of control rats contribution to the DEX treated groups. CrN supplementation did not correct the elevation of blood glucose and insulin caused by DEX. The addition of CrN does not affect the rate of change in triglycerides and cholesterol trained by DEX. It is particularly noted that the addition of CrN has no significant effect on liver and kidney functions.Keywords : chromium, diabetes, glucose, dexamethasone
Ramadan fasting influences on food intake consumption, sleep schedule, body weight and some plasma parameters in healthy fasting volunteers
This study examines the changes in the lifestyle that accompanied Ramadan fasting. For this purpose, we followed the questionnaire programming meals, food consumption and sleep rhythm. We also followed changes in plasma biological parameters. The results show that daily energy consumption was not changed and neither was the body weight. Nevertheless, a decrease in fat mass was detected. Concerning plasma, we found a decrease in blood glucose from the 7th day of fasting. This effect was found also at the 21st day, accompanied by a reduction in insulin levels. Our results on plasma lipids showed a decrease in triglycerides with total cholesterol level unchanged. Furthermore, we noted an increased HDL-C against a decreased LDL-C fraction. Taken together, these results suggest that fasting may induce beneficial effects in blood lipid concentrations.Keywords: Ramadan fasting, food intake consumption, sleep schedule, body weight, plasma parameters, healthy fasting volunteersAfrican Journal of Biotechnology Vol. 12(21), pp. 3327-333
Implausible algorithm output in UK liver transplantation allocation scheme:importance of transparency
Efecto de procesos de ahumado frĂo y caliente y la adiciĂłn de extracto polifenĂłlico natural de Dunaliella salina sobre la calidad bioquĂmica y la vida Ăștil de filetes de Sander lucioperca almacenados durante 90 dĂas
The effects of cold and hot smoking and the addition of Dunaliella salina polyphenol extract on the biochemical quality and shelf-life of Sander lucioperca fillets after storage for 90 days at 0-4 °C were examined. The results showed a significant increase in protein, lipid, free fatty acid, and 1,1-diphenyl-2-picrylhydrazyl contents, and a decrease in peroxide and thiobarbituric acid reactive substances, and volatile base nitrogen levels in cold (CSF) and hot (HSF) smoked fillets covered with or without extract and stored for 1, 20, and 90 days compared to fresh fillets (FF). Saturated and monounsaturated fatty acids exhibited a significant increase in FF and CSF and HSF covered with or without extract. The total polyunsaturated fatty acids revealed a significant decrease in FF and CSF and HSF with or without extract. Therefore, cold and hot smoking and polyphenol extract improved the biochemical quality and storage shelf-life of fillets for 90 days at 0-4 °C.Se examinaron los efectos del ahumado en frĂo y en caliente y la adiciĂłn de extracto de polifenoles de Dunaliella salina sobre la calidad bioquĂmica y la vida Ăștil de filetes de Sander lucioperca almacenados durante 90 dĂas a 0-4 °C. Los resultados mostraron un aumento significativo en los contenidos de proteĂnas, lĂpidos, ĂĄcidos grasos libres y 1,1-difenil-2-picrilhidrazilo, y una disminuciĂłn en las sustancias reactivas de perĂłxido y ĂĄcido tiobarbitĂșrico, y los niveles de nitrĂłgeno bĂĄsico volĂĄtil en frĂo (LCR) y caliente (HSF) de filetes ahumados cubiertos con o sin extracto y almacenados durante 1, 20 y 90 dĂas en comparaciĂłn con los filetes frescos (FF). Los ĂĄcidos grasos saturados y monoinsaturados exhibieron un aumento significativo en FF y LCR y HSF cubiertos con o sin extracto. Los ĂĄcidos grasos poliinsaturados totales revelaron una disminuciĂłn significativa en FF y CSF y HSF con o sin extracto. Por lo tanto, el ahumado en frĂo y en caliente y el extracto de polifenoles mejoraron la calidad bioquĂmica y la vida Ăștil durante el almacenamiento de los filetes durante 90 dĂas a 0-4 °C
ComposiciĂłn bioquĂmica y potencial antioxidante del pepino del mar MediterrĂĄneo comestible Holothuria tubulosa
The sea cucumber or holothurian is a marine species which has been prized in some Asian counÂtries for its nutritional qualities. The purpose of this work was to study the biochemical composition and free radical scavenging and antioxidant activities of Holothuria tubulosa tegument from the Bizerta lagoon in northÂern Tunisia. The obtained data demonstrated that the extract of sea cucumber teguments exhibited high bioÂchemical levels (such as moisture 80.77%, protein 7.07%, lipids 10.21%, energy value 13.64 Kcal/g ww), and an important nutritional value (including n-3/n-6: 2.11, EPA+DHA: 20.96, AI: 1.38 and TI: 0.54). High antiÂoxidant activities were recorded in the integument by the radical scavenging tests of ABTS and DPPH as well as by the total antioxidant capacity and the FRAP in comparison with the BHT standard. Our results showed that H. Tubulosa tegument has high nutritional value with high antioxidant activities and could be considered a nutraceutical product.El pepino de mar o la holoturia es una especie marina apreciada en algunos paĂses asiĂĄtiÂcos por sus cualidades nutricionales. El propĂłsito de este trabajo fue estudiar la composiciĂłn bioquĂmica y las actividades antioxidantes y de eliminaciĂłn de radicales libres del tegumento de Holothuria tubulosa de la laguna de Bizerta, en el norte de TĂșnez. Los datos obtenidos demuestran que el extracto de tegumentos de pepino de mar mostrĂł altos niveles bioquĂmicos (como humedad 80,77%, proteĂna 7,07%, lĂpidos 10,21%, valor energĂ©tico 13,64 Kcal/gww) y un valor nutricional importante (incluyendo n-3/ n-6: 2,11, EPA+DHA: 20,96, AI: 1,38 y TI: 0,54). Se registraron altas actividades antioxidantes en el tegumento mediante las pruebas de eliminaciĂłn de radicales de ABTS y DPPH, asĂ como por la capacidad antioxidante total y el FRAP, y esto, en comparaciĂłn con el estĂĄndar BHT. Nuestros resultados mostraron que el tegumento de H. Tubulosa tiene un valor nutricional importante con una alta actividad antioxidante y podrĂa considerarse un producto nutracĂ©utico
Separation and evaluation of natural antileishmanial potential against Leishmania major and infuntum isolated from the Tunisia strains
Healed Lesions of Human Cutaneous Leishmaniasis Caused By Leishmania major Do Not Shelter Persistent Residual Parasites
In human cutaneous leishmaniasis (HCL) caused by Leishmania (L.) major, the cutaneous lesions heal spontaneously and induce a Th1-type immunity that confers solid protection against reinfection. The same holds true for the experimental leishmaniasis induced by L. major in C57BL/6 mice where residual parasites persist after spontaneous clinical cure and induce sustainable memory immune responses and resistance to reinfection. Whether residual parasites also persist in scars of cured HCL caused by L. major is still unknown. Cutaneous scars from 53 volunteers with healed HCL caused by L. major were biopsied and the tissue sample homogenates were analyzed for residual parasites by four methods: i) microscope detection of amastigotes, ii) parasite culture by inoculation on biphasic medium, iii) inoculation of tissue exctracts to the footpad of BALB/c mice, an inbred strain highly susceptible to L. major, and iv) amplification of parasite kDNA by a highly sensitive real-time PCR (RT-PCR). Our results show that the scars of healed lesions of HCL caused by L. major do not contain detectable residual parasites, suggesting that this form likely induces a sterile cure at least within the scars. This feature contrasts with other Leishmania species causing chronic, diffuse, or recidivating forms of leishmaniasis where parasites do persist in healed lesions. The possibility that alternative mechanisms to parasite persistence are needed to boost and maintain long-term immunity to L. major, should be taken into consideration in vaccine development against L. major infection
UV activation of polymeric high aspect ratio microstructures: Ramifications in antibody surface loading for circulating tumor cell selection
The need to activate thermoplastic surfaces using robust and efficient methods has been driven by the fact that replication techniques can be used to produce microfluidic devices in a high production mode and at low cost, making polymer microfluidics invaluable for in vitro diagnostics, such as circulating tumor cell (CTC) analysis, where device disposability is critical to mitigate artifacts associated with sample carryover. Modifying the surface chemistry of thermoplastic devices through activation techniques can be used to increase the wettability of the surface or to produce functional scaffolds to allow for the covalent attachment of biologics, such as antibodies for CTC recognition. Extensive surface characterization tools were used to investigate UV activation of various surfaces to produce uniform and high surface coverage of functional groups, such as carboxylic acids in microchannels of different aspect ratios. We found that the efficiency of the UV activation process is highly dependent on the microchannel aspect ratio and the identity of the thermoplastic substrate. Colorimetric assays and fluorescence imaging of UV-activated microchannels following EDC/NHS coupling of Cy3-labeled oligonucleotides indicated that UV-activation of a PMMA microchannel with an aspect ratio of ???3 was significantly less efficient toward the bottom of the channel compared to the upper sections. This effect was a consequence of the bulk polymer's damping of the modifying UV radiation due to absorption artifacts. In contrast, this effect was less pronounced for COC. Moreover, we observed that after thermal fusion bonding of the device's cover plate to the substrate, many of the generated functional groups buried into the bulk rendering them inaccessible. The propensity of this surface reorganization was found to be higher for PMMA compared to COC. As an example of the effects of material and microchannel aspect ratios on device functionality, thermoplastic devices for the selection of CTCs from whole blood were evaluated, which required the immobilization of monoclonal antibodies to channel walls. From our results, we concluded the CTC yield and purity of isolated CTCs were dependent on the substrate material with COC producing the highest clinical yields for CTCs as well as better purities compared to PMMA.close9
The management of desmoid tumours: A joint global consensus-based guideline approach for adult and paediatric patients
Abstract Desmoid tumor (DT; other synonymously used terms: Desmoid-type fibromatosis, aggressive fibromatosis) is a rare and locally aggressive monoclonal, fibroblastic proliferation characterised by a variable and often unpredictable clinical course. Previously surgery was the standard primary treatment modality; however, in recent years a paradigm shift towards a more conservative management has been introduced and an effort to harmonise the strategy amongst clinicians has been made. We present herein an evidence-based, joint global consensus guideline approach to the management of this disease focussing on: molecular genetics, indications for an active treatment, and available systemic therapeutic options. This paper follows a one-day consensus meeting held in Milan, Italy, in June 2018 under the auspices of the European Reference Network for rare solid adult cancers, EURACAN, the European Organisation for Research and Treatment of Cancer (EORTC) Soft Tissue and Bone Sarcoma Group (STBSG) as well as Sarcoma Patients EuroNet (SPAEN) and The Desmoid tumour Research Foundation (DTRF). The meeting brought together over 50 adult and pediatric sarcoma experts from different disciplines, patients and patient advocates from Europe, North America and Japan
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