AVALIAÇÃO DO RISCO CARDÍACO, CONFORME ESCORES DE RISCO DE FRAMINGHAM, EM PACIEN- TESAMBULATORIAIS DE SALVADOR DO SUL, SÃO PEDRO DA SERRA E BARÃO - RS

As doenças cardiovasculares constituem a principal causa de mortalidade no mundo e o seu crescimento significativo nos países emdesenvolvimento alerta para um grande impacto nas classes menos favorecidas. O presente trabalho realizado no Laboratório Chiesa,Hospital São Salvador, Salvador do Sul – RS, tem como objetivo, avaliar o percentual de risco para evento coronariano utilizando oEscore de Risco de Framingham (ERF) em parte da população dos municípios de Salvador do Sul, São Pedro da Serra e Barão, nosmeses de setembro, outubro e novembro de 2004. O total de pacientes avaliados no período foi de 354, homens e mulheres, sendo que242 (68%) apresentaram baixo risco, 62 (18%) apresentaram médio risco e 50 (14%) apresentaram alto risco de desenvolver eventocoronariano em 10 anos. Mais de 2/3 dos indivíduos apresentam algum tipo de dislipidemia, sendo que no grupo de alto risco chega à82%. É baixo o percentual de fumantes e neste grupo as dislipidemias não são freqüentes. Diabéticos no grupo de alto risco chegam apraticamente metade dos pacientes. A hipertensão arterial sistêmica atinge cerca de 40% dos pacientes e no grupo de alto risco atinge90%. Estes resultados confirmam a importância e a necessidade de se verificar o perfil lipídico, bem como realizar a estratificação derisco e estabelecer metas lipídicas de tratamento para a aterosclerose nesta comunidade

Projeto de Intervenção: uma Proposta de Formação em EaD

Comunicar é um desafio nos tempos atuais, principalmente no contexto educacional, seja presencial ou a distância. O entrave na comunicação pode ser observado nos processos de orientação realizados na modalidade a distância. O objetivo deste artigo é apresentar a contribuição do professor orientador na construção de um projeto de intervenção em um curso de pós-graduação ofertado na modalidade a distância na busca por pesquisas e práticas que conduzam a uma formação de qualidade. A metodologia utilizada é exploratória, descritiva a partir de um estudo de caso. Como resultado, observou-se que a interação e o uso das mídias, síncronas ou assíncronas, incluindo as redes sociais, contribuem para potencializar a formação do indivíduo como cidadão crítico na sociedade do conhecimento.Palavras-chave: Orientação, Formação, Qualidade, Educação a distância. Intervention Project: a Proposal for Training in Distance EducationAbstractCommunication is a challenge nowadays, especially in education, either face-to-face or distance education. Barriers in communication can be observed in the guidance process in distance education. This paper aims to present the contribution of the advisor teacher in the construction of an intervention project in a distance graduate course, in order to search for researches and practices that lead to a quality education. The methodology is exploratory, descriptive from a case study. As a result, we observed that the interaction and the use of media, synchronous or asynchronous, including social networks, contribute to enhance the training of the individual as a critical citizen in the knowledge society.Keywords: Guidance, Formation, Quality, Distance education

Animal models of metabolic disorders in the study of neurodegenerative diseases : an overview

The incidence of metabolic disorders, as well as of neurodegenerative diseases—mainly the sporadic forms of Alzheimer’s and Parkinson’s disease—are increasing worldwide. Notably, obesity, diabetes, and hypercholesterolemia have been indicated as early risk factors for sporadic forms of Alzheimer’s and Parkinson’s disease. These conditions share a range of molecular and cellular features, including protein aggregation, oxidative stress, neuroinflammation, and blood-brain barrier dysfunction, all of which contribute to neuronal death and cognitive impairment. Rodent models of obesity, diabetes, and hypercholesterolemia exhibit all the hallmarks of these degenerative diseases, and represent an interesting approach to the study of the phenotypic features and pathogenic mechanisms of neurodegenerative disorders. We review the main pathological aspects of Alzheimer’s and Parkinson’s disease as summarized in rodent models of obesity, diabetes, and hypercholesterolemia

Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro

Oxidative modification of low-density lipoprotein (LDL) represents an important factor in atherogenesis. In the present study, we have investigated the antioxidant capability of diphenyl diselenide (PhSe)2, a simple organoseleno compound, against copper (Cu2+) and peroxyl radical-induced human LDL oxidation in vitro. In initial studies using human serum, (PhSe)2 caused a dose-dependent inhibition of Cu2+-induced lipid peroxidation, which was correlated to thiol consumption. (PhSe)2 increased lipid peroxidation lag phase and decreased lipid peroxidation rate in isolated human LDL, evaluated by measuring both conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) levels. Consistent with these observations, (PhSe)2 showed a marked inhibitory effect on 2,2-azobis(2-amidinopropane dihydrochloride) (AAPH)-induced oxidation of LDL or parinaric acid (PnA) incorporated into LDL. (PhSe)2 also displayed a dose-dependent protective effect against Cu2+-induced lipid peroxidation in rat aortic slices. Interestingly, besides the antioxidant effects of (PhSe)2 toward the lipid moieties of LDL, which was related to its thiol-peroxidase activity, protein moieties from human isolated LDL were also protected against Cu2+-induced oxidation. The results presented herein are the first to show that (i) (PhSe)2 inhibits lipid peroxidation in human isolated LDL in vitro, (ii) this phenomenon is related to its thiol-peroxidase activity, and (iii) this chalcogen also prevents the oxidation of protein moieties of human LDL. Taken together, such data render (PhSe)2 a promising molecule for pharmacological studies with respect to the atherogenic process.http://www.sciencedirect.com/science/article/B6T12-4S21TS2-1/1/38250cf8bae4a4195ccd8905cf5c2a8

Innovation Habitat: Sustainable possibilities for the society

Contemporary society is moving in the core of a reality in which sustainability needs to be thought out and inserted through practices carried out in different dimensions of society, such as organizations, public and private institutions. This paper aims to identify the contribution of innovation habitats (IH) for sustainability in society. The methodology used was systematic review of scientific literature in one online database. As a result, it was identified: 47 scientific papers publicated since 2000, but more frequently in the last year, 2014, with 10 publications, without providing a reference author in the area. There was also a high number of papers about management and social sciences. It was noticed a short number of publications, empirical and theoretical, about practices to promote sustainable actions in the society, so this indicates the need of research on this kind of practices, with innovation environment as the driver

Diphenyl diselenide protects neuronal cells against oxidative stress and mitochondrial dysfunction : involvement of the glutathione-dependent antioxidant system

Oxidative stress and mitochondrial dysfunction are critical events in neurodegenerative diseases; therefore, molecules that increase cellular antioxidant defenses represent a future pharmacologic strategy to counteract such conditions. The aim of this study was to investigate the potential protective effect of (PhSe)2 on mouse hippocampal cell line (HT22) exposed to tert-BuOOH (in vitro model of oxidative stress), as well as to elucidate potential mechanisms underlying this protection. Our results showed that tert-BuOOH caused time- and concentration- dependent cytotoxicity, which was preceded by increased oxidants production and mitochondrial dysfunction. (PhSe)2 pre-incubation significantly prevented these cytotoxic events and the observed protective effects were paralleled by the upregulation of the cellular glutathione-dependent antioxidant system: (PhSe)2 increased GSH levels (> 60%), GPx activity (6.9-fold) and the mRNA expression of antioxidant enzymes Gpx1 (3.9-fold) and Gclc (2.3-fold). Of note, the cytoprotective effect of (PhSe)2 was significantly decreased when cells were treated with mercaptosuccinic acid, an inhibitor of GPx, indicating the involvement of GPx modulation in the observed protective effect. In summary, the present findings bring out a new action mechanism concerning the antioxidant properties of (PhSe)2. The observed upregulation of the glutathione-dependent antioxidant system represents a future pharmacologic possibility that goes beyond the well-known thiol-peroxidase activity of this compound

Integração ensino e serviço no contexto da pandemia de COVID-19: relato de experiência da práxis dos residentes sanitaristas na vigilância epidemiológica

Introduction: Initially identified in the city of Wuhan, China in 2019, Coronavirus Disease 2019 (COVID-19), demonstrated itself with a high dissemination power. Due to the potential for contagion, the World Health Organization recommended isolation measures to countries, avoiding agglomerations, reducing the incidence of cases. Vitória de Santo Antão, a municipality located near the Metropolitan Region of Recife, required in this context an essential role of epidemiological surveillance to develop contingency strategies and actions in this pandemic health scenario. Objective: To report the experience of health residents working in the epidemiological surveillance sector during the COVID-19 pandemic. Method: Experience report carried out by health residents of the Multiprofessional Residency Program for the Interiorization of Health Care to strengthen epidemiological surveillance in the face of the pandemic in the municipality of Vitória de Santo Antão. Results: Two forms were created via Google Forms in order to register and monitor cases of influenza syndrome, SRAG and COVID-19 in the municipality. The creation of daily epidemiological bulletins for the dissemination of data was adopted by the health surveillance sector, presenting the most pertinent information obtained from the monitoring of cases of SRAG, COVID-19 and Flu Syndrome of the local population, always comparing with the state and federal scenarios. Conclusions: The pandemic scenario brought about a readjustment of residents’ activities, imposing on them the challenge of adapting to a new epidemiological context, envisioning a look at the needs of the territories and their learning process, strengthening the surveillance actions during the scenario of pandemic.Introdução: Inicialmente identificada na cidade de Wuhan, na China em 2019, a Coronavirus Disease 2019 (COVID-19) demonstrou-se com um alto poder de disseminação. Devido à potencialidade de contágio, a Organização Mundial da Saúde recomendou aos países medidas de isolamento para evitar aglomerações e diminuir a incidência de casos. Vitória de Santo Antão, município localizado próximo à Região Metropolitana de Recife, neste contexto requereu imprescindivelmente uma atuação da vigilância epidemiológica para elaborar ações e estratégias de contingência nesse cenário sanitário pandêmico. Objetivo: Relatar a experiência dos residentes sanitaristas na atuação no setor da vigilância epidemiológica durante o enfrentamento da pandemia de COVID-19. Método: Relato de experiência realizado pelos residentes sanitaristas do Programa de Residência Multiprofissional de Interiorização de Atenção à Saúde para fortalecimento da vigilância epidemiológica no enfrentamento da pandemia no município de Vitória de Santo Antão. Resultados: Foram criados dois formulários via Google Forms com o objetivo de registrar e acompanhar os casos de síndrome gripal, síndrome respiratória aguda grave (SRAG) e COVID-19 no município. Foi adotada pelo setor de vigilância em saúde a criação de boletins epidemiológicos diários para divulgação dos dados, apresentando as informações mais pertinentes obtidas a partir do monitoramento dos casos de síndrome gripal, SRAG e COVID-19 da população local, sempre comparando com o cenário estadual e federal. Conclusões: O cenário de pandemia suscitou uma readequação das atividades dos residentes, ao imprimir sobre esses o desafio de se adequar a um novo contexto epidemiológico, gerando um olhar sobre as necessidades dos territórios e do seu processo de aprendizagem e fortalecendo as ações de vigilância durante o cenário de pandemia

High cholesterol diet exacerbates blood-brain barrier disruption in LDLr–/– mice : impact on cognitive function

Background: Evidence has revealed an association between familial hypercholesterolemia and cognitive impairment. In this regard, a connection between cognitive deficits and hippocampal blood-brain barrier (BBB) breakdown was found in low-density lipoprotein receptor knockout mice (LDLr–/–), a mouse model of familial hypercholesterolemia. Objective: Herein we investigated the impact of a hypercholesterolemic diet on cognition and BBB function in C57BL/6 wild-type and LDLr–/– mice. Methods: Animals were fed with normal or high cholesterol diets for 30 days. Thus, wild-type and LDLr–/– mice were submitted to memory paradigms. Additionally, BBB integrity was evaluated in the mice’s prefrontal cortices and hippocampi. Results: A tenfold elevation in plasma cholesterol levels of LDLr–/– mice was observed after a hypercholesterolemic diet, while in wild-type mice, the hypercholesterolemic diet exposure increased plasma cholesterol levels only moderately and did not induce cognitive impairment. LDLr–/– mice presented memory impairment regardless of the diet. We observed BBB disruption as an increased permeability to sodium fluorescein in the prefrontal cortices and hippocampi and a decrease on hippocampal claudin-5 and occludin mRNA levels in both wild-type and LDLr–/– mice treated with a hypercholesterolemic diet. The LDLr–/– mice fed with a regular diet already presented BBB dysfunction. The BBB-increased leakage in the hippocampi of LDLr–/– mice was related to high microvessel content and intense astrogliosis, which did not occur in the control mice. Conclusion: Therefore, LDLr–/– mice seem to be more susceptible to cognitive impairments and BBB damage induced by exposure to a high cholesterol diet. Finally, BBB disruption appears to be a relevant event in hypercholesterolemia-induced brain alterations

Potential neuroprotective and antiinfammatory efects provided by omega-3 (DHA) against Zika virus infection in human SH-SY5Y cells

Zika virus (ZIKV) has a strong tropism for the nervous system and has been related to post-infection neurological syndromes. Once neuronal cells are infected, the virus is capable of modulating cell metabolism, leading to neurotoxicity and cellular death. The negative efect of ZIKV in neuron cells has been characterized. However, the description of molecules capable of reversing these cytotoxic efects is still under investigation. In this context, it has been largely demonstrated that docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid, is highly neuroprotective. Here, we hypothesized that DHA’s neuroprotective proprieties could have an infuence on ZIKV-induced neurotoxicity in SHSY5Y cells. Our data showed that pre-treatment of SH-SY5Y cells with DHA increased the cell viability and proliferation in ZIKV-infected cells. Moreover, DHA triggered an anti-infammatory response in those infected cells. Besides, DHA was capable of restoring mitochondria function and number in ZIKVinfected SH-SY5Y cells. In addition, cells pre-treated with DHA prior to ZIKV infection presented a lower viral load at diferent times of infection. Taking together, these results demonstrated that DHA has a potential anti-infammatory and neuroprotective efect against ZIKV infection in these neuron-like cells and could be a useful tool in the treatment against this virus