Aislamiento y caracterización de distintas poblaciones celulares en el tejido adiposo humano

Las células madre adultas, han sido empleadas en numerosos procedimientos de terapia clínica, ya que no desencadenan rechazo inmunológico y tampoco presentan problemas éticos relacionados con su uso. El tejido adiposo, constituye una fuente idónea para la extracción de células madre mesenquimales, debido a su abundancia y al bajo riesgo quirúrgico que conlleva su extracción. El peligro de transmisión de zoonosis que existe al emplear reactivos de origen animal en el procesamiento y cultivo de células humanas que podrían llegar a ser trasplantadas a pacientes, produjo que nos planteásemos sustituir reactivos animales empleados en los procedimientos tradicionales, por otros de origen humano o sintéticos. Los objetivos de esta tesis doctoral son por un lado, el diseño de un protocolo de aislamiento de células madre del tejido adiposo humano sin emplear reactivos de origen animal que permita acercar su uso a la clínica. Además, también se ha llevado a cabo el aislamiento y caracterización de distintas subpoblaciones celulares presentes en la fracción conseguida empleando el protocolo diseñado

Buenas prácticas ambientales para el consumo de papel

Se describen someramente las características del proceso de fabricación del papel que tienen un impacto sobre el medio ambiente, así como la forma en la que se puede minimizar o mitigar ese impacto y las etiquetas ambientales que garantizan una fabricación sostenibleBellver Navarro, CG. (2013). Buenas prácticas ambientales para el consumo de papel. http://hdl.handle.net/10251/33734

Buenas prácticas ambientales para la adquisición o alquiler de vehículos

En la Guía se describen los principales aspectos ambientales derivados del uso y mantenimiento de vehículos a motor, así como los impactos ambientales asociados y se presentan las opciones más respetuosas con el medio ambiente. También se trata el mecanismo de gestión de este tipo de vehículos al final de su vida útilBellver Navarro, CG. (2015). Buenas prácticas ambientales para la adquisición o alquiler de vehículos. http://hdl.handle.net/10251/58228

The Problems of Violence in Adolescents. Inability of the parents to transmit values?

És una realitat innegable que cada vegada apareix més informació als mitjans de comunicació i a la producció científica que ens adverteix que hi ha un increment de violència i de manca de valors entre els joves, sense aconseguir entendre què passa amb aquests joves o, si bé, ens trobem davant d’una generació de mares i pares incompetents per a educar en una societat titllada de deshumanitzada, tecnològica i global. Per això, l’objectiu d’aquest article és partir d’interrogants i cercar explicacions en estudis de l’espectre científic sobre el grau o tipus d’influència que exerceixen els progenitors, des dels seus estils educatius, en el desenvolupament prosocial versus antisocial de la seva progènie, contemplant variables biològiques, psicològiques i educatives que puguin donar-nos noves respostes.Es una realidad innegable que cada vez aparece más información en los medios de comunicación y en la producción científica que nos advierte de un incremento de la violencia y de falta de valores entre los jóvenes, sin lograr comprender qué sucede con ellos o, si bien, estamos ante una generación de madres y padres incompetentes para educar en una sociedad tildada de deshumanizada, tecnológica y global. Por ello, el objetivo de este artículo es partir de interrogantes y buscar explicaciones en estudios del espectro científico acerca del grado o tipo de influencia que ejercen los progenitores, desde sus estilos educativos, en el desarrollo prosocial versus antisocial de su progenie, contemplando variables biológicas, psicológicas y educativas que puedan darnos nuevas respuestas.It is an undeniable fact that there is an increasing amount of information in the media and in scientific publications warning of a rise in levels of violence and an absence of values among young people, accompanied by a lack of understanding of what is happening with these youngsters or whether we dealing with a generation of parents who are incapable of bringing up their children in a society that can be described as dehumanized, technological and global. In light of this, the aim of this arti cle is to seek explanations, on the basis of pertinent questions, in a wide range of scientific studies about the kind and amount of influence exercised by parents through their styles of parenting in the prosocial rather than antisocial development of their children, taking into consideration a range of biological, psychology and educational variables that may provide us with new ways of responding

XIII Simposio Hispano-Portugués de Relaciones Hídricas en las Plantas

The effect of water stress on grape berry ripening and quality has been extensively investigated during the last decades (Chaves et al., 2010; Lovisolo et al., 2010; Kuhn et al., 2013, Zarrouk et al., 2016). Overall, berry quality benefits from mild to moderate water deficit and the conventional wisdom says that the observed positive effects of water deficits are due to low yield and small berries. But more recently water deficit was shown to profoundly alter berry secondary metabolism, particularly of flavonoids, thus greatly regulating the ripening process. The regulation of genes and proteins of the various metabolic pathways is either the consequence of a direct effect of water shortage and/or indirect via the changing of the light environment around grape clusters due to the impairment of vine vegetative growth. The matter is far from being conclusively addressed and two major causes can be highlighted: the experimental set-ups adopted in the majority of experiments, namely the water stress timing and conditions (pre- or post-véraison water stress); and the diversity of varieties (Zarrouk et al., 2016). Grape berries are non-climacteric fruits wherein abscisic acid (ABA) is central to the ripening process. The differential increase of anthocyanin compounds in berries under pre- or post-véraison deficit irrigation is primarily attributed to large differences in abscisic acid (ABA) sensitivity displayed by different varieties as a consequence of large differences in the ability to regulate water losses through chemical (i.e. ABA) signaling. Grape berries thus represent a unique situation in which abiotic stress and developmental signaling intersect via the activities of ABA. In addition, climate condition, namely high temperature along the growing season is considered a putative constraint to the implementation and success of the deficit irrigation regime. Also the interaction elevated temperature – water deficit is considered as the main cause of complexity of field experiments results namely in what concerns the ripening of wine grapes. During the last ten years, our investigation aimed to comprehend the impact of water stress and heat stress on grape berry ripening, skin secondary metabolism as well as cuticle properties. We have investigated ABA-mediated cross talk between abiotic stress and ripening as it relates to flavonoid biosynthesis in the grape berry skin. We show that the mild XIII Simposio Hispano-Portugués de Relaciones Hídricas en las Plantas: Pamplona 18-20 Octubre 2016 7 stress imposed by deficit irrigation have a positive impact on berry composition only when high temperature is not a limiting factor. We also show that the biological function of ABA is under berry developmental control and does not correlate with vine water status or berry temperature until a certain water stress threshold. It also appears that ABA catabolism/conjugation processes are implicated in the ripening process and in water and heat stresses responses. In addition, our most recent results in cuticle indicate that water and heat stresses increase berry wax accumulation across berry development, affecting cuticle morphology, composition and transpiration. The recent data related to metabolic changes and hormonal control of grape berry ripening as well as berry cuticle properties under deficit irrigation will be discussed and limitations in the interpretation of results will be identified

La teoría del aprendizaje social como modelo explicativo de la violencia filio-parental

In opposition to what it was kept before the decade of the 70s of the 20th century, the familiar context is one of the most violent, being able to give the parent-child violence, in the pair, between brothers and the child-parent violence. In this article we centre on the violence of teenagers against their progenitors being the first aim of our review to delimit and to define so much the term of familiar violence as much as of the adolescent violence towards parents to achieve to understand some of the reasons that gain to explain this phenomenon from the theory of the social learning. This theory is presented as an explanatory model suggested by almost totality of the authors who study and investigate the child-parents violence. To do so we have used a bibliographic review in qualitative analytic version, in base at investigations and books from the decade of the 70s until the year 2011 that have studied the child-parents violence in some cases linked with others types of family violence.En contra de lo que se mantenía hasta la década de los 70 del siglo pasado, el contexto familiar es el más violento, puesto que en éste puede darse la violencia parento-filial, en la pareja, entre hermanos y la violencia filio-parental. En este artículo nos centramos en la violencia de los adolescentes contra sus progenitores siendo el primer objetivo delimitar y definir tanto el término de la violencia familiar como el de la filio-parental para lograr comprender algunas de las causas que consigan explicar este fenómeno a partir de la teoría del aprendizaje social. Esta teoría se presenta como un modelo explicativo sugerido por casi la totalidad de los autores que estudian e investigan la violencia filio-parental desde distintas disciplinas y perspectivas. Para ello, hemos realizado una revisión bibliográfica, en versión analítico-cualitativa, a partir de investigaciones y libros desde la década de los años 70 hasta el año 2011, que han estudiado la violencia de los hijos/as menores de edad contra sus madres y/o padres, en algunos casos vinculada a otros tipos de violencia en la familia

Evaluating the creative potencial of digital storytelling APPs for Primary Education

This study evaluates the creative potential of different apps (N=20) oriented to the de-sign of digital storytelling (DST), directed to users from 6 to 12 years, using the CREAPP K6-12 instrument, made of 48 indicators related to the 5 dimensions of creativity: flexibility, originality, fluency, product elaboration and problem solving, co-edition and dissemination. After adopting descriptive and multivariate statistical techniques, the results show that 76.6% of these apps have great flexibility, 73.4% favor originality, 72.4% present resources and function-alities endowing them with great fluidity, 68.3% encourage the creation of creative products, 56.5% promote the resolution of problems, yet only 36.3% prevail the co-edition and dissemination of stories in networks. The correlations show that the most flexible app stimulates originality and fluency, propitiates problem solving and promotes the elaboration of creative products. The index of creative potentiality of the apps is calculated, establishing a competitiveness ranking, which offers keys to the Primary teachers to elaborate didactic proposals that promote creativity through the design of DST

Buenas prácticas ambientales para la compra de equipos informáticos

La guía presenta los principales criterios ambientales a tener en cuenta cuando se adquieren equipamientos informáticos. Enumera las etiquetas ambientales más extendidas y proporciona indicaciones sobre el impacto ambiental generado por los equipamientos informáticos a lo largo de todo su ciclo de vidaBellver Navarro, CG.; Pérez Díaz, P. (2022). Buenas prácticas ambientales para la compra de equipos informáticos. http://hdl.handle.net/10251/18012

Aminoacids and flavonoids profiling in tempranillo berries can be modulated by the arbuscular mychorrhizal fungi

(1) Background: Vitis vinifera L. cv. Tempranillo is cultivated over the world for its wine of high quality. The association of Tempranillo with arbuscular mycorrhizal fungi (AMF) induced the accumulation of phenolics and carotenoids in leaves, affected the metabolism of abscisic acid (ABA) during berry ripening, and modulated some characteristics and quality aspects of grapes. The objective of this study was to elucidate if AMF influenced the profiles and the content of primary and secondary metabolites determinants for berry quality in Tempranillo. (2) Methods: Fruit-bearing cuttings inoculated with AMF or uninoculated were cultivated under controlled conditions. (3) Results: Mycorrhizal symbiosis modified the profile of metabolites in Tempranillo berries, especially those of the primary compounds. The levels of glucose and amino acids clearly increased in berries of mycorrhized Tempranillo grapevines, including those of the aromatic precursor amino acids. However, mycorrhizal inoculation barely influenced the total amount and the profiles of anthocyanins and flavonols in berries. (4) Conclusions: Mycorrhizal inoculation of Tempranillo grapevines may be an alternative to the exogenous application of nitrogen compounds in order to enhance the contents of amino acids in grapes, which may affect the aromatic characteristics of wines