Three-dimensional arrangement of elastic fibers in the human corneal stroma

This is the final version of the article. Available from the publisher via the DOI in this record.The cornea is the main refracting lens in the eye. As part of the outer tunic it has to be resilient, a property conferred by the organisation of the constituent collagen. It also has to be sufficiently elastic to regain its exact shape when deformed, in order not to distort the retinal image. The basis of this elasticity is not fully understood. The purpose of this study was to characterise in three dimensions the arrangement and distribution of elastic fibers in the human corneal stroma, using serial block face scanning electron microscopy. We have demonstrated that there exists a complex network of elastic fibers that appear to originate in the sclera or limbus. These appear as elastic sheets in the limbus and peripheral cornea immediately above the trabecular meshwork which itself appears to extend above Descemet's membrane in the peripheral stroma. From these sheets, elastic fibers extend into the cornea; moving centrally they bifurcate and trifurcate into narrower fibers and are concentrated in the posterior stroma immediately above Descemet's membrane. We contend that elastic sheets will play an important role in the biomechanical deformation and recovery of the peripheral cornea. The network may also have practical implications for understanding the structural basis behind a number of corneal surgeries.We would like to thank Dr Anthony Hayes and Mr Derek Scarborough for help with the histology presented in this study and Dr Sally Hayes for useful discussions concerning data interpretation. This work was funded by a Programme Grant (503626) from the Medical Research Council (to KMM) and an MRC studentship (to TW). We thank the CTC Eye Bank at Bristol, UK and the HDBR at Newcastle, UK, for supply of human corneas. The authors have no conflicts of interest to declare

Ultrahigh energy cosmic rays from shocks in the lobes of powerful radio galaxies

The origin of ultrahigh energy cosmic rays (UHECRs) has been an open question for decades. Here, we use a combination of hydrodynamic simulations and general physical arguments to demonstrate that UHECRs can in principle be produced by diffusive shock acceleration (DSA) in shocks in the backflowing material of radio galaxy lobes. These shocks occur after the jet material has passed through the relativistic termination shock. Recently, several authors have demonstrated that highly relativistic shocks are not effective in accelerating UHECRs. The shocks in our proposed model have a range of non-relativistic or mildly relativistic shock velocities more conducive to UHECR acceleration, with shock sizes in the range 1-10 kpc. Approximately 10 per cent of jet's energy flux is focused through a shock in the backflow of M > 3. Although the shock velocities can be low enough that acceleration to high energy via DSA is still efficient, they are also high enough for the Hillas energy to approach 1019-20 eV, particularly for heavier CR composition and in cases where fluid elements pass through multiple shocks. We discuss some of the more general considerations for acceleration of particles to ultrahigh energy with reference to giant-lobed radio galaxies such as Centaurus A and Fornax A, a class of sources which may be responsible for the observed anisotropies from UHECR observatories

1.8 billion years of detrital zircon recycling calibrates a refractory part of Earth’s sedimentary cycle

Detrital zircon studies are providing new insights on the evolution of sedimentary basins but the role of sedimentary recycling remains largely undefined. In a broad region of northwestern North America, this contribution traces the pathway of detrital zircon sand grains from Proterozoic sandstones through Phanerozoic strata and argues for multi-stage sedimentary recycling over more than a billion years. As a test of our hypothesis, integrated palynology and detrital zircon provenance provides clear evidence for erosion of Carboniferous strata in the northern Cordillera as a sediment source for Upper Cretaceous strata. Our results help to calibrate Earth's sedimentary cycle by showing that recycling dominates sedimentary provenance for the refractory mineral zircon

Gene silencing in tick cell lines using small interfering or long double-stranded RNA

Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for RNAi-mediated gene silencing in tick cells need to be established. Ten cell lines from four economically important ixodid tick genera (Amblyomma, Hyalomma, Ixodes and Rhipicephalus including the sub-species Boophilus) were used to examine key parameters including small interfering RNA (siRNA), double stranded RNA (dsRNA), transfection reagent and incubation time for silencing virus reporter and endogenous tick genes. Transfection reagents were essential for the uptake of siRNA whereas long dsRNA alone was taken up by most tick cell lines. Significant virus reporter protein knockdown was achieved using either siRNA or dsRNA in all the cell lines tested. Optimum conditions varied according to the cell line. Consistency between replicates and duration of incubation with dsRNA were addressed for two Ixodes scapularis cell lines; IDE8 supported more consistent and effective silencing of the endogenous gene subolesin than ISE6, and highly significant knockdown of the endogenous gene 2I1F6 in IDE8 cells was achieved within 48 h incubation with dsRNA. In summary, this study shows that gene silencing by RNAi in tick cell lines is generally more efficient with dsRNA than with siRNA but results vary between cell lines and optimal parameters need to be determined for each experimental system

The hierarchical response of human corneal collagen to load

Fibrillar collagen in the human cornea is integral to its function as a transparent lens of precise curvature, and its arrangement is now well-characterised in the literature. While there has been considerable effort to incorporate fibrillar architecture into mechanical models of the cornea, the mechanical response of corneal collagen to small applied loads is not well understood. In this study the fibrillar and molecular response to tensile load was quantified using small and wide angle X-ray scattering (SAXS/WAXS), and digital image correlation (DIC) photography was used to calculate the local strain field that gave rise to the hierarchical changes. A molecular scattering model was used to calculate the tropocollagen tilt relative to the fibril axis and changes associated with applied strain. Changes were measured in the D-period, molecular tilt and the orientation and spacing of the fibrillar and molecular networks. These measurements were summarised into hierarchical deformation mechanisms, which were found to contribute at varying strains. The change in molecular tilt is indicative of a sub-fibrillar “spring-like” deformation mechanism, which was found to account for most of the applied strain under physiological and near-physiological loads. This deformation mechanism may play an important functional role in tissues rich in fibrils of high helical tilt, such as skin and cartilage

Scaling of Entanglement close to a Quantum Phase Transitions

In this Letter we discuss the entanglement near a quantum phase transition by analyzing the properties of the concurrence for a class of exactly solvable models in one dimension. We find that entanglement can be classified in the framework of scaling theory. Further, we reveal a profound difference between classical correlations and the non-local quantum correlation, entanglement: the correlation length diverges at the phase transition, whereas entanglement in general remains short ranged.Comment: 4 pages, 4 figures, revtex. Stylistic changes and format modifie

Engaging with community researchers for exposure science: lessons learned from a pesticide biomonitoring study

A major challenge in biomonitoring studies with members of the general public is ensuring their continued involvement throughout the necessary length of the research. The paper presents evidence on the use of community researchers, recruited from local study areas, as a mechanism for ensuring effective recruitment and retention of farmer and resident participants for a pesticides biomonitoring study. The evidence presented suggests that community researchers' abilities to build and sustain trusting relationships with participants enhanced the rigour of the study as a result of their on-the-ground responsiveness and flexibility resulting in data collection beyond targets expected

Chytrid epidemics may increase genetic diversity of a diatom spring-bloom

Contrary to expectation, populations of clonal organisms are often genetically highly diverse. In phytoplankton, this diversity is maintained throughout periods of high population growth (that is, blooms), even though competitive exclusion among genotypes should hypothetically lead to the dominance of a few superior genotypes. Genotype-specific parasitism may be one mechanism that helps maintain such high-genotypic diversity of clonal organisms. Here, we present a comparison of population genetic similarity by estimating the beta-dispersion among genotypes of early and peak bloom populations of the diatom Asterionella formosa for three spring-blooms under high or low parasite pressure. The Asterionella population showed greater beta-dispersion at peak bloom than early bloom in the 2 years with high parasite pressure, whereas the within group dispersion did not change under low parasite pressure. Our findings support that high prevalence parasitism can promote genetic diversification of natural populations of clonal hosts