Identifying genes related to choriogenesis in insect panoistic ovaries by Suppression Subtractive Hybridization

<p>Abstract</p> <p>Background</p> <p>Insect ovarioles are classified into two categories: panoistic and meroistic, the later having apparently evolved from an ancestral panoistic type. Molecular data on oogenesis is practically restricted to meroistic ovaries. If we aim at studying the evolutionary transition from panoistic to meroistic, data on panoistic ovaries should be gathered. To this end, we planned the construction of a Suppression Subtractive Hybridization (SSH) library to identify genes involved in panoistic choriogenesis, using the cockroach <it>Blattella germanica </it>as model.</p> <p>Results</p> <p>We constructed a post-vitellogenic ovary library by SSH to isolate genes involved in choriogenesis in <it>B. germanica</it>. The tester library was prepared with an ovary pool from 6- to 7-day-old females, whereas the driver library was prepared with an ovary pool from 3- to 4-day-old females. From the SSH library, we obtained 258 high quality sequences which clustered into 34 unique sequences grouped in 19 contigs and 15 singlets. The sequences were compared against non-redundant NCBI databases using BLAST. We found that 44% of the unique sequences had homologous sequences in known genes of other organisms, whereas 56% had no significant similarity to any of the databases entries. A Gene Ontology analysis was carried out, classifying the 34 sequences into different functional categories. Seven of these gene sequences, representative of different categories and processes, were chosen to perform expression studies during the first gonadotrophic cycle by real-time PCR. Results showed that they were mainly expressed during post-vitellogenesis, which validates the SSH technique. In two of them corresponding to novel genes, we demonstrated that they are specifically expressed in the cytoplasm of follicular cells in basal oocytes at the time of choriogenesis.</p> <p>Conclusion</p> <p>The SSH approach has proven to be useful in identifying ovarian genes expressed after vitellogenesis in <it>B. germanica</it>. For most of the genes, functions related to choriogenesis are postulated. The relatively high percentage of novel genes obtained and the practical absence of chorion genes typical of meroistic ovaries suggest that mechanisms regulating chorion formation in panoistic ovaries are significantly different from those of meroistic ones.</p

Supranutritional doses of vitamin E to improve lamb meat quality

Vitamin E is a fat-soluble antioxidant, therefore, it can be stored in any fat depot in the body, where it exerts a potent chain-breaking antioxidant effect. Moreover, the antioxidant activity of vitamin E-like compounds is also present in meat post mortem. The deposition of tocopherol in the muscle depends on the dosage, the source, and the period of supplementation, so different dosage-time combinations have been developed. Vitamin E does not affect production parameters if minimum requirements for function and growth are satisfied, but it could influence lamb fatty acid profile. During display, it protects PUFA from degradation, reducing lipid oxidation and, therefore, delaying discolouration. Furthermore, vitamin E would indirectly affect the development of lamb aroma by reducing protein and lipid oxidation. To facilitate optimal supplementation rates by producers and retailers, the present paper reviews vitamin E chemistry, biochemistry, nutrition and its ability to maintain lamb quality

Brownie, a Gene Involved in Building Complex Respiratory Devices in Insect Eggshells

Background: Insect eggshells must combine protection for the yolk and embryo with provisions for respiration and for the entry of sperm, which are ensured by aeropyles and micropyles, respectively. Insects which oviposit the eggs in an egg-case have a double problem of respiration as gas exchange then involves two barriers. An example of this situation is found in the cockroach Blattella germanica, where the aeropyle and the micropyle are combined in a complex structure called the sponge-like body. The sponge-like body has been well described morphologically, but nothing is known about how it is built up. Methodology/Principal Findings: In a library designed to find genes expressed during late chorion formation in B. germanica, we isolated the novel sequence Bg30009 (now called Brownie), which was outstanding due to its high copy number. In the present work, we show that Brownie is expressed in the follicle cells localized in the anterior pole of the oocyte in late choriogenesis. RNA interference (RNAi) of Brownie impaired correct formation of the sponge-like body and, as a result, the egg-case was also ill-formed and the eggs were not viable. Conclusions/Significance: Results indicate that the novel gene Brownie plays a pivotal role in building up the sponge-lik

Gestión del conocimiento de Diseño en la industria del mueble

Ponencia presentada en el VIII Congreso Internacional de Ingeniería de Proyectos celebrada en Bilbao en el año 2004The goal of the project was to improve the design process of the products in the furniture industry. It consisted of investigating the definition of a knowledge-based model in order to assist in the design of the industrial products. Firstly, a design process analysis and redesign was made through the process modeling. From the current design process model of the studied company a new design process model was achieved. In the new process, changes and consequences of introducing a new design tool, such as Catia V5, were reflected. Improvements referring to times, workflows, reduction of mistakes and better efficiency in the product design were achieved. Then, an Informal Model was built up and developed with the product knowledge of the company. This model was the necessary way between the information that defines the product related with the company and the Formal Model, which is required for its application in computer programs for products design. Finally, through using the program Catia V5, the prototype of a model was built up. That model allowed the achievement of variations in current designs automatically, taking into account the features and limitations of itself. Those automatic designs were gotten by the user, modifying the defined variables by introducing the searched parameters. In that way, a reduction of the time needed for current design and less mistakes of any type were achieved. As a whole, the project achieved a reduction of times and mistakes, increased flexibility referred to changes in the product design and integration between the design tasks, and achieved a best product knowledge management in the company.El objetivo general de este proyecto era mejorar el proceso de diseño de los productos de la industria del mueble. Consistía en investigar en la definición de un modelo basado en el conocimiento para asistir el diseño de productos industriales. En primer lugar se realizó un análisis y rediseño del proceso de diseño actual de la empresa estudiada mediante el modelado de procesos. A partir del modelo de proceso actual se obtuvo un nuevo modelo, en el cual se analizaban y reflejaban los cambios y consecuencias en el proceso ante la implementación de una nueva herramienta de diseño, como es el Catia. Se obtuvieron mejoras en aspectos de tiempo, flujo de actividades, reducción de fallos y una mayor eficiencia en el diseño. Otro de los puntos del proyecto fue la construcción de un Modelo Informal, desarrollado con el conocimiento de producto de la empresa, el cual constituye el paso necesario entre la información que define el producto en relación con la empresa y la obtención del Modelo Formal, requerido para su aplicación en herramientas informáticas para el diseño de productos. Y por último, mediante la utilización de la aplicación Catia V5 se desarrolló un prototipo de un modelo que permite la obtención de variaciones en diseños desarrollados rutinariamente de manera automática, teniendo en cuenta las características y limitaciones del mismo. Esto se produce, cuando el usuario introduce los parámetros deseados, modificando así las variables definidas, produciendo de esta forma una reducción de tiempos y minimización en cualquier tipo de error. Con todo ello se consigue una minimización de tiempos y errores, aumentar la flexibilidad en cuanto a la introducción de cambios en el diseño, aumentar la integración entre las actividades de producción y las actividades de diseño y una mejor gestión del conocimiento del producto por parte de la organización

Effect of fluorine and nitrogen content on the properties of Ca-Mg-Si-Al-O-(N)-(F) glasses

X-ray amorphous glasses of composition (in equivalent percent) 15Ca:15Mg: 55Si:15Al:(100-x-y)O:xN:yF with x=0, 10, 15 and y=0, 1, 3, 5, were prepared by melting and casting. The effects of oxygen substitution by fluorine and/or nitrogen on the physical, mechanical, thermal and optical properties of the glasses have been investigated. Molar volume, fractional glass compactness, microhardness, Young's Modulus, glass-transition temperature, dilatometric-softening point and refractive index increased linearly with nitrogen substitution for oxygen, whereas molar volume and thermal expansion coefficient decreased linearly with nitrogen increase. In contrast, all properties except glass-transition temperature and dilatometric-softening point, are virtually unaffected by fluorine substitution for oxygen. Significant and linear, decreases in thermal properties occurred with increasing fluorine substitution level. All the data collected and its analysis clearly showed that the substitution effects of fluorine for oxygen on the studied properties of the glasses of the system with general formula Ca-Mg-Si-Al-O-(N)-(F) are totally independent and additive with respect to the substitution effects of nitrogen for oxygen on glass properties

Variabilidad de vibrios planctónicos y epifíticos en un ambiente costero afectado por proliferaciones de Ostreopsis

Vibrios include several pathogenic bacteria that occur in aquatic environments. The presence of Vibrio has been assessed in many ecosystems by culture-based techniques. However, little is known on the contribution of Vibrios in the sea, especially in areas subject to harmful algal blooms. A preliminary study in Sant Andreu de Llavaneres beach (NW Mediterranean) showed the presence of some Vibrio species during a recurrent bloom of the harmful benthic dinoflagellate Ostreopsis cf. ovata. In order to establish the importance of Vibrios in a coastal area of the NW Mediterranean and to study the association with the dinoflagellate, we conducted a sampling monitoring for one year to quantify the concentration of Vibrios both in the water (free-living and attached to particles) and in the epiphytic community of macroalgae. The aims were 1) to evaluate the relative abundance of Vibrio in the epiphytic and in the planktonic bacterial community, 2) to assess the percentage of free-living and attached Vibrios in the planktonic community, and 3) to determine whether the presence of Vibrios is associated with the blooms of the toxic dinoflagellate Ostreopsis or with other environmental parameters. For this purpose, a CARD-FISH molecular probe was applied for the specific detection of bacteria belonging to the genus Vibrio. Cells were quantified and the abundance of both particles and bacteria attached to particles were assessed. The maximum Vibrio concentration (1.3x104 cells ml–1 and 1.4x106 cells g–1 FW, for planktonic and epiphytic samples, respectively) was detected in September. Free-living Vibrios contributed 0.38±0.24% to the total free-living planktonic community and 1.12±0.28% to the epiphytic bacterial community. However, their contribution was particularly high in the planktonic community attached to particles (17.37±20.49%). Although in the planktonic community Vibrio was found preferentially free-living (82.63±20.01%), particles are a niche for Vibrios, since in particles Vibrios may represent up to 72% of the total attached bacterial community. Abundance of planktonic Vibrio was correlated with Ostreopsis concentration and it is likely that they play a role in the wound infections suffered by beach users during the bloom.El género Vibrio incluye a varias bacterias patogénicas que se encuentran en ecosistemas acuáticos. La presencia de Vibrio se ha estimado en muchos ecosistemas mediante técnicas basadas en cultivos. Sin embargo, se conoce poco sobre la contribución de Vibrios en el mar, especialmente en áreas afectadas por proliferaciones algales nocivas. Un estudio preliminar en la playa de Sant Andreu de Llavaneres (Mediterráneo NO) mostró la presencia de algunas especies de Vibrio durante una proliferación recurrente del dinoflagelado béntico nocivo Ostreopsis cf. ovata. Para poder establecer la relevancia de los Vibrios en un área costera del Mediterráneo NO y estudiar su asociación con el dinoflagelado, realizamos un muestreo de monitoreo durante un año para cuantificar la concentración de Vibrios tanto en el agua (de vida libre y adheridos a partículas) y en la comunidad epifítica de macroalgas con los objetivos de 1) evaluar la abundancia relativa de Vibrio en la comunidad bacteriana tanto planctónica como epifítica, 2) estimar el porcentaje de Vibrios de vida libre y adheridos a partículas en la comunidad bacteriana planctónica y 3) determinar si la presencia de Vibrios está relacionada con las proliferaciones del dinoflagelado Ostreopsis o con otros parámetros ambientales. Para este propósito, se aplicó una sonda molecular de CARD-FISH para la detección específica de bacterias pertenecientes al género Vibrio. Se cuantificaron las células y también la abundancia de partículas y de las bacterias adheridas a estas partículas. La máxima concentración de Vibrio (1.3x104 cels ml–1 y 1.4x106 cels g–1 PF, para muestras planctónicas y epifíticas, respectivamente) fue detectada en Septiembre. Los Vibrios de vida libre contribuyeron un 0.38±0.24% al total de la comunidad bacteriana de vida libre y un 1.12±0.28% a la comunidad bacteriana epifítica. Sin embargo, su contribución fue especialmente elevada en la comunidad bacteriana adherida a partículas (17.37±20.49%). Aunque en la comunidad planctónica Vibrio se encontraba preferentemente no adheridos a partículas (82.63±20.01%), las partículas constituyen un nicho para Vibrios, ya que pueden llegar a representar hasta un 72% de la comunidad bacteriana adherida a partículas. La abundancia de Vibrio en el plancton se correlacionó con la concentración de Ostreopsis, y es posible que éstos jueguen un papel en las infecciones de heridas que sufren los bañistas durante las proliferaciones algales

Spanish nursing and medical students' knowledge, confidence and willingness about COVID-19: A cross-sectional study

Background: COVID-19 challenges world governments. In Spain, measures to contain the pandemic are novel, and include the possibility of contracting Nursing and Medical students who might not be ready or willing to treat infected cases. Objectives: To study Spanish Nursing and Medical students' knowledge about COVID-19 community transmission prevention measures, treating infected patients, and their confidence and willingness to treat cases. To learn their views about the first public health policy measures adopted by the Spain government to contain the pandemic. Design: Cross-sectional study. Settings and participants: 237 Spanish Nursing and Medical students. Methods: An online questionnaire was designed on the knowledge, confidence and willingness to treat, and the suitability of the public health policy measures adopted in Spain. The data were collected through social net-works. The results were compared according to their university degree. Results: Knowledge about community prevention was suitable, unlike knowledge about treating infected patients. Students had little confidence in treating cases, but their willingness and moral responsibility were high. Very few significant differences were found in their university degrees. Medical students evaluated the measures taken in Spain more favorably. Conclusions: Students' knowledge about COVID-19 community prevention measures was adequate, but not about preventive measures when treating patients with COVID-19. They felt little confidence despite being willing to treat infected patients. The sample agreed with the public health measures adopted in Spain