A novel approach to bioelectrical impedance plethysmography for the assessment of arterial and venous circulatory problems in the forearm

Peripheral vascular disease (PVD) and/or peripheral arterial disease (PAD) are sicknesses known to inadequate delivery of either arterial or venous blood towards the extremities. Such sickness may trigger complications owing to the lack of transport of oxygen and nutrients, thus causing hypoxic events that may eventually prompt to ischaemic tissue or even the loss of the compromised limb. One of the most prominent indicators of prosperous health is blood volume and flow. The basic information within these health parameters may show cardiovascular problems or the advance of further complications related to other diseases like diabetes. In clinical setting, there effective methods to measure these parameters like Doppler ultrasound, photoplethysmography or venous occlusion plethysmography. These methods take measurements from either single vessels and/or small volume of tissue. However, it is difficult to establish a relation between the obstruction of arterial and/or venous circulation and the amount of blood received by the tissue. Bioelectrical impedance plethysmography (iPG) measures blood changes by driving a small amount of AC current into the body and after measuring the potential created by fluids flowing through tissue. This technique apart from taking measures within defined volumes of tissue, it is easy to use as only needs four electrodes on the skin. Hence, a bespoken bioelectrical impedance device including hardware and software was built ready to measure changes in blood volume/flow in the upper limbs. The system was assessed in an in-vivo controlled environment with 8 participants. The blood flow towards their left arms was altered by constricting the upper arm with a cuff at three levels: 1) below venous pressure 2) amongst venous and arterial pressure and 3) during total occlusion. Simultaneously, measurements from various instruments like ECG, Doppler ultrasound, laser Doppler flowmetry and PPG were taken and compared to the measurements obtained from the iPG instrument and defining its correlation with the impedimetric signal. The results from the experiments showed that the bioelectrical impedance signal changed in basal and arterial pulses showing specific characteristics for each kind of occlusion. The data indicated that it is possible to differentiate between a venous and arterial occlusion by examining both components of the impedance signal. The impedance during venous occlusion dropped in average 0.658±0.230% from the baseline. On the other hand, during arterial occlusion the base impedance dropped in a higher rate approximately 1.13±04.82%, indicating a differentiator during both type of blood flow disruption. Furthermore, the impedance plethysmography waveform morphology also reshaped during these occlusive periods. The whole waveform during artificial venous obstruction increased in magnitude, the systolic peak rose 31.80%, the dicrotic notch 47.73% and the diastolic point 31.92%, where the value of the latter was higher than the dicrotic notch point. In contrast, in the time of partial arterial occlusion the waveform also increased in size at all these points, but its shape was altered. The impedance magnitude at the diastolic point went below the ones at the dicrotic notch. These fluctuations provided additional further information that it might be possible to differentiate amongst venous and arterial occlusions. By consolidating the data obtained by the iPG device, it is possible to produce an index ratio between the basal impedance and these three reference points which may help to identify early circulatory problems in the arterial and/or venous systems

Geminivirus Rep Protein Interferes with the Plant DNA Methylation Machinery and Suppresses Transcriptional Gene Silencing

Viruses are masters at circumventing host defenses and manipulating the cellular environment for their own benefit. The replication of the largest known family of single-stranded DNA viruses, Geminiviridae, is impaired by DNA methylation but the fact that plants might use methylation as a defense against geminiviruses and the impact that viral genome methylation may have during the infection, remain controversial. We have found that geminiviruses reduce the expression of the plant maintenance DNA methyltransferases, MET1 and CMT3, in both, locally and systemically infected tissues. Furthermore, we demonstrated that the virus-mediated repression of these two maintenance DNA methyltransferases is widely spread among different geminivirus species and we have identified Rep as the geminiviral protein responsible for the repression of MET1 and CMT3. The presence of Rep, suppresses transcriptional gene silencing (TGS) of an Arabidopsis transgene and of host loci whose expression is strongly controlled by MET1. Bisulfite sequencing analyses showed that the expression of Rep caused a substantial reduction in the levels of DNA methylation at certain loci at CG sites. The biological relevance of these findings and the role of Rep as a TGS suppressor will be discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Novel scheme for a PCM-based cold energy storage system. Design, modelling, and simulation

This paper studies the design and dynamic modelling of a novel thermal energy storage (TES) system combined with a refrigeration system based on phase change materials (PCM). Cold-energy production supported by TES systems is a very appealing field of research, since it allows flexible cold-energy management, combining demand fulfilment with cost reduction strategies. The paper proposes and compares two different simulation models for a cold-energy storage system based on PCM. First, a continuous model is developed, the application of which is limited to decoupled charging/discharging operations. Given such conditions, it is a relatively precise model, useful for the tuning of the TES parameters. The second proposed model is a discrete one, which, despite implementing a discrete approximation of the system behaviour, allows to study more general conditions, such as series of partial charging/discharging operations. Simulation results of both models are compared regarding decoupled charging/discharging operations, and the ability of the discrete model to represent more realistic partial operations is analysed.Comment: 48 pages, 14 figures. Postprint of the final published wor

Dilaton thin-shell wormholes supported by a generalized Chaplygin gas

In this article, we construct spherical thin-shell wormholes with charge in dilaton gravity. The exotic matter required for the construction is provided by a generalized Chaplygin gas. We study the stability under perturbations preserving the symmetry. We find that the increase of the coupling between the dilaton and the electromagnetic fields reduces the range of the parameters for which stable configurations are possible.Comment: 14 pages, 6 figures. v3: typos correcte

Importance of the temperature in the interaction plant-geminivirus

Introduction. Geminivirusare plant viruses that have circular single-stranded DNA (ssDNA) genome encapsidated in twinned quasi-icosahedral (geminate) virionsand are a serious threat to diverse economically important crops such as tomato, pepper and cassava. TYLCV (Tomato yellow leaf curl virus) belongs to the genus begomovirus which is by far, the largest of the seven genera in the family Geminiviridae and it is a pathogen that induces a devastating disease in tomato in the Mediterranean region. Cultured tomatoes are often exposed to a combination of extreme heat and infection with TYLCV and this combination leads to intense disease symptoms and yield losses.The impact of temperature over the interaction tomato- TYLCVhas been characterized (Ghandi et al., 2016) but these results seem to be contradictory to our findings. Objective. The main objective of this work is to improve our understanding of the impact that high temperature has in the interaction plant-geminivirus.Material and methods. Nicotianabenthamiana plants were infected with TYLCV and growth in a chamber at 21 ºC or at 35 ºC. The amount of virus, symptoms, plant height and number of leaves were monitored at 14, 21 and 28 dpi (days post-infection). Three biological replicates were performed, each containing 15 infected plants and 10 non-infected plants (mock). Results. No differences were observed between non-infected and infected plants at both temperatures, in plant height or in the number of leaves, but the amount of virus and the severity of symptoms were significantly reduced in plants at high temperature. Conclusions. We conclude that TYLCV yield decreases in N. benthamiana due to high temperature and ongoing experiments in tomato TYLCV-infected plants will let us determine the importance of this finding in an agronomical important crop and to compare our results with the published data.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Optimal control analysis and Practical NMPC applied to refrigeration systems

This work is focused on optimal control of mechanical compression refrigeration systems. A reduced-order state-space model based on the moving boundary approach is proposed for the canonical cycle, which eases the controller design. The optimal cycle (that satisfying the cooling demand while maximizing efficiency) is defined by three variables, but only two inputs are available, therefore the controllability of the proposed model is studied. It is shown through optimization simulations how optimal cycles for a range of the cooling demand turn out not to be achieved by keeping the degree of superheating to a minimum. The Practical NMPC and a well-known feedback-plus-feedforward strategy from the literature are compared in simulation, both showing trouble in reaching the optimal cycle, which agrees with the controllability study.Comment: 39 pages, 14 figures. Postprint of the final published wor

MINLP-based hybrid strategy for operating mode selection of TES-backed-up refrigeration systems

This brief deals with the satisfaction of the daily cooling demand by a hybrid system that consists of a vapour-compression refrigeration cycle and a thermal energy storage (TES) unit, based on phase change materials. The addition of the TES tank to the original refrigeration plant allows to schedule the cooling production regardless of the instantaneous demand, given that the TES tank can store cold energy and release it whenever deemed appropriate. The scheduling problem is posed as an optimization problem based on mixed-integer non-linear programming (MINLP), since it includes both discrete and continuous variables. The latter corresponds to the references on the main cooling powers involved in the problem (cooling production at the evaporator and TES charging/discharging), whereas the discrete variables define the operating mode scheduling. Therefore, in addition to the hybrid features of the physical plant, a hybrid optimal control strategy is also proposed. A receding horizon approach is applied, similar to model predictive control (MPC) strategies, while economic criteria are imposed in the objective function, as well as feasibility issues. The TES state estimation is also addressed, since its instantaneous charge ratio is not measurable. The proposed strategy is applied in simulation to a challenging cooling demand profile and the main advantages of the MINLP-based strategy over a non-linear MPC-based scheduling strategy previously developed are highlighted, regarding operating cost, ease of tuning, and ability to adapt to cooling demand variations.Comment: 22 pages, 11 figures. Postprint of the final published wor

Nonlinear model predictive control-based guidance law for path following of unmanned surface vehicles

This work proposes a nonlinear model predictive control-based guidance strategy for unmanned surface vehicles, focused on path following. The application of this strategy, in addition to overcome drawbacks of previous line-of-sight-based guidance laws, intends to enable the application of predictive strategies also to the low-level control, responsible for tracking the references provided by the guidance strategy. The stability and robustness of the proposed strategy are theoretically discussed. Furthermore, given the non-negligible computational cost of such nonlinear predictive guidance strategy, a practical nonlinear model predictive control strategy is also applied in order to reduce the computational cost to a great extent. The effectiveness and advantages of both proposed strategies over other nonlinear guidance laws are illustrated through a complete set of simulations.Comment: 21 pages, 15 figures. Postprint of the final published wor

Novel scheme for a PCM-based cold energy storage system. Design, modelling, and simulation

This paper studies the design and dynamic modelling of a novel thermal energy storage (TES) system combined with a refrigeration system based on phase change materials (PCM). Cold-energy production supported by TES systems is a very appealing field of research, since it allows flexible cold-energy management, combining demand fulfilment with cost reduction strategies. The paper proposes and compares two different simulation models for a cold-energy storage system based on PCM. First, a continuous model is developed, the application of which is limited to decoupled charging/discharging operations. Given such conditions, it is a relatively precise model, useful for the tuning of the TES parameters. The second proposed model is a discrete one, which, despite implementing a discrete approximation of the system behaviour, allows to study more general conditions, such as series of partial charging/discharging operations. Simulation results of both models are compared regarding decoupled charging/discharging operations, and the ability of the discrete model to represent more realistic partial operations is analysed.This is the accepted version of the articleMinisterio de Ciencia e Innovació

The perception of indole negatively modulates biocontrol activities in the plant beneficial Rhizobacterium Lysobacter capsici AZ78

The rhizosphere is a dynamic environment characterised by multiple and complex microbial interactions where diffusible communication signals (DCS) continuously influence the expression patterns of the microbiome, hence regulating fundamental traits for adaptation to the rhizosphere. In particular, plant-associated bacteria release indole, a Volatile Organic Compound (VOC) that acts as an interkingdom signal able to influence antibiotic resistance, motility, biofilm formation and virulence. Lysobacter spp. are commonly found in the rhizosphere and have been frequently associated to disease suppression. For instance, the biocontrol activity of the plant beneficial bacterium Lysobacter capsici AZ78 (AZ78) has been reported against the phytopathogenic oomycetes Phytophthora infestans, Plasmopara viticola, Pythium ultimum and the Gram-positive bacterium Rhodococcus fascians. However, there is scarce information about Lysobacter spp. ecology and how DCS, and in particular indole, may affect their behaviour in the rhizosphere. To investigate the aspects determining rhizosphere competence and functioning of Lysobacter spp., this work presents a functional and transcriptomic analysis performed on AZ78, which was grown in the presence indole. The presence of indole significantly reduced the inhibition capacity of AZ78 against P. ultimum and R. fascians by 47 and 31%, respectively. Moreover, RNA-Seq analysis revealed that nearly 12% of all genes in AZ78 genome were modulated by indole. In particular, indole downregulated the expression of the heat-stable antifungal factor (HSAF) biosynthetic gene cluster, which may affect AZ78 antioomycete and antimicrobial activity. Moreover, in the presence of indole, AZ78 downregulated several signal transduction pathways responsible for nutrients uptake, resulting in reduced growth. Finally, indole downregulated several genes related to type IV pilus functionality, which might lead to impaired twitching motility. This study sheds light on the key role of DCS such as indole in shaping AZ78 behaviour in the rhizosphere and suggests that, manipulating DCS levels may alter the persistence and functioning of several plant-beneficial rhizobacteria, such as Lysobacter strains