Herbivore effect traits and their impact on plant community biomass: an experimental test using grasshoppers

1. Using trait-based approaches to study trophic interactions may represent one of the most promising approaches to evaluate the impact of trophic interactions on ecosystem functioning. To achieve this goal, it is necessary to clearly identify which traits determine the impact of one trophic level on another.2. Using functionally contrasting grasshopper species, we tested the ability of multiple traits (morphological, chemical and biomechanical) to predict herbivore impact on the biomass of a diverse plant community. We set up a cage experiment in an old species rich grassland field and evaluated how multiple candidate grasshopper effect traits mediated herbivore impact on plant biomass.3. Grasshoppers had different impact on plant community biomass (consuming up to 60 % of plant community biomass). Grasshopper impact was positively correlated with their incisive strength while body size or grasshopper C:N ratio exhibited low predictive ability. Importantly, the strong relationship between the incisive strength and the impact was mediated by the grasshopper feeding niche, which was well predicted in our study by two simple plant traits (leaf dry matter content, leaf C:N ratio). Feeding niche differences between grasshoppers were explained by differences in incisive strength, highlighting the fundamental linkage between grasshopper effect traits and their niche.4. Our study contributes to the development of the trait-based approach in the study of trophic interactions by providing a first experimental test of the relationship between herbivore effect traits, their impact on plant community biomass, and in a larger extent on ecosystem functioning. By comparing the relative importance of multiple interacting grasshopper traits, our study showed that incisive strength was a key effect trait which determined grasshopper feeding niche and its relative impact on plant community biomass

Caracterização do processo de rigor mortis do músculo Ilio-ischiocaudalis de jacaré-do-pantanal (Caiman crocodilus yacare) e maciez da carne Characterization of rigor mortis process of muscle Ilio-ischiocaudalis of pantanal alligator (Caiman crocodilus yacare) and meat tenderness

Este trabalho utilizou seis carcaças de jacaré-do-pantanal (Caiman crocodilus yacare) com o objetivo de caracterizar o processo de rigor mortis do músculo Ílio-ischiocaudalis durante o resfriamento industrial e avaliar a maciez dessa carne. Os jacarés foram escolhidos aleatoriamente e abatidos na Cooperativa de Criadores do Jacaré do Pantanal (COOCRIJAPAN), Cáceres, Mato Grosso. Após a sangria, aferiu-se as variações das temperaturas da câmara de resfriamento, das carcaças e o pH. Foram colhidas amostras para determinação do comprimento de sarcômero, da força de cisalhamento e perdas por cozimento em diferentes intervalos de tempo (0,5, 3, 5, 7, 10, 12, 15, 24 e 36h). A temperatura da câmara de resfriamento variou de 2,6&deg;C (0,5h) a 0,9&deg;C (36h) e a temperatura média das carcaças variou de 21,0&deg;C a 4,2&deg;C, respectivamente. O pH médio inicial do músculo foi de 6,7 e o final 5,6 e a contração máxima do sarcômero do músculo Ilio-ischiocaudalis ocorreu na 15ª hora após a sangria (1,5µm). Essa carne apresentou força de cisalhamento menor que 6,0kg.<br>This paper studied six pantanal alligators (Caiman crocodilus yacare) carcass with goal of rigor mortis process characterization of Ilio-ischiocaudalis muscle during industrial cooling and meat tenderness. The alligators were randomly assembled and slaughtered at Cooperativa de Criadores do Jacaré do Pantanal (COOCRIJAPAN) - Cáceres- Mato Grosso After exsanguination, were mensured temperature of chilling room and carcasses, pH and samples were collected for determination the sarcomere length, shear force and cooking loss at different times (0.5, 3, 5, 7, 10, 12, 15, 24 and 36 hours). The temperature of chilling room varied from 2.6&deg;C (0.5h) to 0.9&deg;C (36h) and the mean carcass temperature from 21.0&deg;C to 4.2&deg;C, respectively. The mean initial pH of the muscle was 6.7 and the final was 5.6. The smallest sarcomere size ocurred at 15 hours after exsanguination (1.5µm). This meat presents shear force lower than 6.0kg

Consumo, digestibilidade aparente, produção e composição do leite e variáveis ruminais em vacas leiteiras alimentadas com dietas à base de cana-de-açúcar Intake, apparent digestibility, milk production and composition and ruminal variables of dairy cows fed sugar cane based diets

Doze vacas da raça Holandesa, puras e mestiças, foram distribuídas em três quadrados latinos 4 x 4, com o objetivo de avaliar o consumo e a digestibilidade aparente dos nutrientes, a produção e composição do leite, o pH e a amônia ruminais. As dietas foram constituídas à base de silagem de milho (SM) (AG 1051) com relação volumoso:concentrado (V:C) de 60:40, com base na matéria seca (MS), ou à base de cana-de-açúcar (CA) (RB 855536) com relação V:C de 60:40, com 0,35 ou 1% da mistura uréia+sulfato de amônio (SA) ou V:C de 50:50 com 1% de uréia+SA. O consumo de MS foi de 17,8; 14,9; 14,4 e 15,8 kg/dia, para as dietas, respectivamente. O maior consumo de MS resultou, também, em maior consumo de nutrientes para a dieta à base de SM, com exceção dos carboidratos não-fibrosos, em que se verificou maior consumo para a dieta com CA com 50% de concentrado. A produção de leite (PL) foi maior para a dieta à base de SM, não havendo diferença na PL entre as dietas contendo CA, sendo de 22,0; 19,0; 18,6 e 20,1 kg/dia e a produção de leite corrigida para 3,5% de gordura foi de 23,0; 19,7; 19,4 e 21,3 kg/dia para as dietas SM, CA 0,35%, CA 1%, CA 1% 50:50, respectivamente. Em relação à composição do leite, não foram encontradas diferenças entre as dietas. Os teores de gordura foram de 3,8, 3,8, 3,8 e 3,9% para as dietas, respectivamente. Não foram verificadas diferenças quanto à digestibilidade aparente da MS, matéria orgânica, proteína bruta e carboidratos totais entre as dietas. A digestibilidade aparente da fibra em detergente neutro (FDN) foi maior para a dieta contendo SM (47%). Não houve diferença na digestibilidade da FDN, entre as diferentes dietas com CA, 32, 31 e 31%, respectivamente. Dentro de cada tempo (antes e três horas após a alimentação matinal), não houve diferença nos valores de pH e compostos nitrogenados amoniacais (N-NH3) entre as dietas.<br>Twelve purebred and crossbred Holstein cows were allotted to three 4 x 4 Latin Squares to evaluate the intake, apparent digestibility of nutrients, milk production and composition, ruminal pH and ammonia. The experimental diets were based on corn silage (CS) (AG 1051) with forage:concentrate ratio (F:C) of 60:40, in dry matter (DM) basis, or based on sugar cane (SC) (RB 855536) with F:C of 60:40 with .35 or 1% of urea+ammonium sulfate (AS) mixture or F:C of 50:50 with 1% of urea+AS. DM intake was 17.8, 14.9, 14.4, and 15.8 kg/day, for the diets, respectively. DM intake was higher, resulting in greater nutrient intake for CS based diet, except for the non fiber carbohydrates intake, which showed higher intake for SC based diet with 50% concentrate. Milk production (MP) was higher for the CS based diet, with no difference in MP among the SC based diets, with mean values of 22.0, 19.0, 18.6, and 20.1 kg/day and the 3.5% fat corrected milk production was 23.0, 19.7, 19.4, and 21.3 kg/day for the CS, SC .35%, SC 1% and SC 1% 50:50, respectively. Milk composition was not different among the experimental diets and milk fat contents were 3.8, 3.8, 3.8, and 3.9% for the CS, SC .35%, SC 1% and SC 1% 50:50, respectively. No difference on apparent digestibility of DM, organic matter, crude protein and total carbohydrates among the diets was observed. The neutral detergent fiber (NDF) apparent digestibility was higher for the CS based diet (47). There was no difference in NDF digestibility among SC based diets, 32, 31 and 31%, respectively. Inside each time (before and three hours after the morning feeding) there was no difference of pH values and amonia nitrogen compounds (N-NH3) among the diets

The evolutionary ecology of fatty-acid variation : Implications for consumer adaptation and diversification

The nutritional diversity of resources can affect the adaptive evolution of consumer metabolism and consumer diversification. The omega-3 long-chain polyunsaturated fatty acids eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3) have a high potential to affect consumer fitness, through their widespread effects on reproduction, growth and survival. However, few studies consider the evolution of fatty acid metabolism within an ecological context. In this review, we first document the extensive diversity in both primary producer and consumer fatty acid distributions amongst major ecosystems, between habitats and amongst species within habitats. We highlight some of the key nutritional contrasts that can shape behavioural and/or metabolic adaptation in consumers, discussing how consumers can evolve in response to the spatial, seasonal and community-level variation of resource quality. We propose a hierarchical trait-based approach for studying the evolution of consumers' metabolic networks and review the evolutionary genetic mechanisms underpinning consumer adaptation to EPA and DHA distributions. In doing so, we consider how the metabolic traits of consumers are hierarchically structured, from cell membrane function to maternal investment, and have strongly environment-dependent expression. Finally, we conclude with an outlook on how studying the metabolic adaptation of consumers within the context of nutritional landscapes can open up new opportunities for understanding evolutionary diversification