163 research outputs found
Experimental and modelling approach to the legume-Rhizobium interaction: test of plant-host sanctions in co-inoculated plants with fixing and non-fixing strains
Ponencia presentada en la II Conferencia Iberoamericana de Interacciones Beneficiosas Microorganismo-Planta-Ambiente (IBEMPA). XIV Reunión Nacional de la Sociedad Española de Fijación de Nitrógeno (SEFIN). XXVI Reunión Latinoamericana de Rizobiología (ALAR). III Congreso Hispano-Portugués de Fijación de Nitrógeno. “Microorganismos para una Agricultura de futuro”. Sevilla, España, 2 al 6 de septiembre de 2013We tested the plant host sanction hypothesis using soybean plants co-inoculated with two rhizobial strains, a normally N2 fixing strain and a mutant derivative that lacks nitrogenase activity but has the same nodulation abilities. We found no evidence of functioning plant host sanctions to cheater rhizobia based on nodular rhizobia viability in co-inoculated plants.Fil: Marco, Diana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.Fil: Marco, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: Talbi, Choura. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Departamento de Microbiología del Suelo y Sistemas Simbióticos; España.Fil: Bedmar, Eulogio J. Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín. Departamento de Microbiología del Suelo y Sistemas Simbióticos; España
Effects of Soil Biota from Different Ranges on Robinia Invasion: Acquiring Mutualists and Escaping Pathogens
The net effects of soil biota on exotic invaders can be variable, in part, because net effects are produced by many interacting mutualists and antagonists. Here we compared mutualistic and antagonistic biota in soils collected in the native, expanded, and invasive range of the black locust tree, Robinia pseudoacacia. Robinia formed nodules in all soils with a broad phylogenetic range of N-fixing bacteria, and leaf N did not differ among the different sources of soil. This suggests that the global expansion of Robinia was not limited by the lack of appropriate mutualistic N-fixers. Arbuscular mycorrhizal fungi (AMF) from the native range stimulated stronger positive feedbacks than AMF from the expanded or invasive ranges, a biogeographic difference not described previously for invasive plants. Pythium taxa collected from soil in the native range were not more pathogenic than those from other ranges; however, feedbacks produced by the total soil biota were more negative from soils from the native range than from the other ranges, overriding the effects of AMF. This suggests that escape from other pathogens in the soil or the net negative effects of the whole soil community may contribute to superior performance in invaded regions. Our results suggest that important regional evolutionary relationships may occur among plants and soil biota, and that net effects of soil biota may affect invasion, but in ways that are not easily explained by studying isolated components of the soil biota
Bacterial Communities in the Rhizosphere of Amilaceous Maize (Zea mays L.) as Assessed by Pyrosequencing
Maize (Zea mays L.) is the staple diet of the native peasants in the Quechua region of the
Peruvian Andes who continue growing it in small plots called chacras following ancestral
traditions. The abundance and structure of bacterial communities associated with the
roots of amilaceous maize has not been studied in Andean chacras. Accordingly, the
main objective of this study was to describe the rhizospheric bacterial diversity of
amilaceous maize grown either in the presence or the absence of bur clover cultivated
in soils from the Quechua maize belt. Three 16S rRNA gene libraries, one corresponding
to sequences of bacteria from bulk soil of a chacra maintained under fallow conditions,
the second from the rhizosphere of maize-cultivated soils, and the third prepared from
rhizospheric soil of maize cultivated in intercropping with bur clover were examined using
pyrosequencing tags spanning the V4 and V5 hypervariable regions of the gene. A total
of 26031 sequences were found that grouped into 5955 distinct operational taxonomic
units which distributed in 309 genera. The numbers of OTUs in the libraries from the
maize-cultivated soils were significantly higher than those found in the libraries from bulk
soil. One hundred ninety seven genera were found in the bulk soil library and 234 and
203 were in those from the maize and maize/bur clover-cultivated soils. Sixteen out of
the 309 genera had a relative abundance higher than 0.5% and the were (in decreasing
order of abundance) Gp4, Gp6, Flavobacterium, Subdivision3 genera incertae sedis
of the Verrucomicrobia phylum, Gemmatimonas, Dechloromonas, Ohtaekwangia,
Rhodoferax, Gaiella, Opitutus, Gp7, Spartobacteria genera incertae sedis, Terrimonas,
Gp5, Steroidobacter and Parcubacteria genera incertae sedis. Genera Gp4 and Gp6
of the Acidobacteria, Gemmatimonas and Rhodoferax were the most abundant in bulk
soil, whereas Flavobacterium, Dechloromonas and Ohtaekwangia were the main genera
in the rhizosphere of maize intercropped with bur clover, and Gp4, Subdivision3 genera
incertae sedis of phylum Verrucomicrobia, Gp6 and Rhodoferax were the main genera
in the rhizosphere of maize plants. Taken together, our results suggest that bur clover
produces specific changes in rhizospheric bacterial diversity of amilaceous maize plants.Peer reviewe
Identification of rhizobial strains nodulating Egyptian grain legumes
The presence of apothecia in mixed species (vegetatively reproducing lichens, occasionally producing ascomata) has been interpreted as a mechanism to increase genetic variability in mostly clonal populations. However, spore viability from these apothecia has not been studied. We asked whether ascospores of the mixed species Physconia grisea are viable and thereby contribute to increasing the genetic diversity within populations of this species. An ontogenetic study of spores in cultures of P. grisea and a related sexual species (P. distorta), showed that although mature apothecia from both species produced and discharged meiospores capable of germination, spores from P. grisea were only rarely (0.43 %) able to continue development whereas those from P. distorta germinated and developed successfully. The strongly reduced viability of P. grisea spores suggested that they do not have a strong reproductive function, at least in the two local populations analyzed. Additionally, we show that the segregation of Physconia grisea ssp. lilacina does not have molecular support. [Int Microbiol 2013; 16(3):145-155]Keywords: Physconia spp. · apothecia · sexual reproduction · germination · ontogenetic development · mixed specie
Effects of nitrate contamination and seasonal variation on the denitrification and greenhouse gas production in La Rocina stream (Doñana National Park, SW Spain)
Climatic influence (global warming and decreased rainfall) could lead to an increase in the ecological
and toxicological effects of the pollution in aquatic ecosystems, especially contamination from agricultural
nitrate (NO3
−) fertilizers. Physicochemical properties of the surface waters and sediments of four
selected sites varying in NO3
− concentration along La Rocina Stream, which feeds Marisma del Rocio in
Do˜nana National Park (South West, Spain), were studied. Electrical conductivity, pH, content in macro
and microelements, total organic carbon and nitrogen, and dissolved carbon and nitrogen were affected
by each sampling site and sampling time. Contaminant NO3
− in surface water at the site with the highest
NO3
− concentration (ranged in 61.6–106.6mgL−1) was of inorganic origin, most probably from chemical
fertilizers, as determined chemically (90% of the total dissolved nitrogen from NO3
−) and by isotopic analysis
of ı15N-NO3
−. Changes in seasonal weather conditions and hydrological effects at the sampling sites
were also responsible for variations in some biological activities (dehydrogenase, -glucosidase, arylsulphatase,
acid phosphatase and urease) in sediments, as well as in the production of the greenhouse gases
CO2, CH4 and N2O. Both organic matter and NO3
− contents influenced rates of gas production. Increased
NO3
− concentration also resulted in enhanced levels of potential denitrification measured as N2O production.
The denitrification process was affected by NO3
− contamination and the rainfall regimen, increasing
the greenhouse gases emissions (CO2, CH4 and especially N2O) during the driest season in all sampling
sites studied.This work was supported by grants CGL2006-06870 and
CTM2009-1473-C02-02 from Ministerio de Ciencia e Innovación
(Spain) and RNM-4746 from Consejería de Innovación, Ciencia y
Empresa de la Junta de Andalucía (Spain), all of them co-financed by
the European Regional Development Fund (ERDF). Support of Junta
de Andalucía to Research Group BIO-275 is also acknowledged. D.
David Correa thanks Ministerio de Educación for predoctoral grant
AP2007-03967.Peer reviewe
The global response regulator RegR controls expression of denitrification genes in Bradyrhizobium japonicum
Bradyrhizobium japonicum
RegSR regulatory proteins belong to the family of two-component regulatory systems, and
orthologs are present in many Proteobacteria where they globally control gene expression mostly in a redox-responsive
manner. In this work, we have performed a transcriptional profiling of wild-type and
regR
mutant cells grown under anoxic
denitrifying conditions. The comparative analyses of wild-type and
regR
strains revealed that almost 620 genes induced in
the wild type under denitrifying conditions were regulated (directly or indirectly) by RegR, pointing out the important role
of this protein as a global regulator of denitrification. Genes controlled by RegR included
nor
and
nos
structural genes
encoding nitric oxide and nitrous oxide reductase, respectively, genes encoding electron transport proteins such as
cycA
(blr7544) or
cy
2
(bll2388), and genes involved in nitric oxide detoxification (blr2806-09) and copper homeostasis (
copCAB
), as
well as two regulatory genes (bll3466, bll4130). Purified RegR interacted with the promoters of
norC
(blr3214),
nosR
(blr0314), a
fixK
-like gene (bll3466), and bll4130, which encodes a LysR-type regulator. By using fluorescently labeled
oligonucleotide extension (FLOE), we were able to identify two transcriptional start sites located at about 35 (P1) and 22 (P2)
bp upstream of the putative translational start codon of
norC
. P1 matched with the previously mapped 5
9
end of
norC
mRNA
which we demonstrate in this work to be under FixK
2
control. P2 is a start site modulated by RegR and specific for anoxic
conditions. Moreover, qRT-PCR experiments, expression studies with a
norC-lacZ
fusion, and heme
c
-staining analyses
revealed that anoxia and nitrate are required for RegR-dependent induction of
nor
genes, and that this control is
independent of the sensor protein Reg
Rhizobium etli Produces Nitrous Oxide by Coupling the Assimilatory and Denitrification Pathways
More than two-thirds of the powerful greenhouse gas nitrous oxide (N2O) emissions from soils can be attributed to microbial denitrification and nitrification processes. Bacterial denitrification reactions are catalyzed by the periplasmic (Nap) or membrane-bound (Nar) nitrate reductases, nitrite reductases (NirK/cd1Nir), nitric oxide reductases (cNor, qNor/ CuANor), and nitrous oxide reductase (Nos) encoded by nap/nar, nir, nor and nos genes, respectively. Rhizobium etli CFN42, the microsymbiont of common bean, is unable to respire nitrate under anoxic conditions and to perform a complete denitrification pathway. This bacterium lacks the nap, nar and nos genes but contains genes encoding NirK and cNor. In this work, we demonstrated that R. etli is able to grow with nitrate as the sole nitrogen source under aerobic and microoxic conditions. Genetic and functional characterization of a gene located in the R. etli chromosome and annotated as narB demonstrated that growth under aerobic or microoxic conditions with nitrate as nitrogen source as well as nitrate reductase activity requires NarB. In addition to be involved in nitrate assimilation, NarB is also required for NO and N2O production by NirK and cNor, respectively, in cells grown microoxically with nitrate as the only N source. Furthermore, β-glucuronidase activity from nirK::uidA and norC::uidA fusions, as well as NorC expression and Nir and Nor activities revealed that expression of nor genes under microoxic conditions also depends on nitrate reduction by NarB. Our results suggest that nitrite produced by NarB from assimilatory nitrate reduction is detoxified by NirK and cNor denitrifying enzymes that convert nitrite into NO which in turn is reduced to N2O, respectively
An integrated biochemical system for nitrate assimilation and nitric oxide detoxification in Bradyrhizobium japonicum
Rhizobia are recognized to establish N(2)-fixing symbiotic interactions with legume plants. Bradyrhizobium japonicum, the symbiont of soybeans, can denitrify and grow under free-living conditions with nitrate (NO(3)(−)) or nitrite (NO(2)(−)) as sole nitrogen source. Unlike related bacteria that assimilate NO(3)(−), genes encoding the assimilatory NO(3)(−) reductase (nasC) and NO(2)(−) reductase (nirA) in B. japonicum are located at distinct chromosomal loci. The nasC gene is located with genes encoding an ABC-type NO(3)(−) transporter, a major facilitator family NO(3)(−)/NO(2)(−) transporter (NarK), flavoprotein (Flp) and single-domain haemoglobin (termed Bjgb). However, nirA clusters with genes for a NO(3)(−)/NO(2)(−)-responsive regulator (NasS-NasT). In the present study, we demonstrate NasC and NirA are both key for NO(3)(−) assimilation and that growth with NO(3)(−), but not NO(2)(−) requires flp, implying Flp may function as electron donor to NasC. In addition, bjgb and flp encode a nitric oxide (NO) detoxification system that functions to mitigate cytotoxic NO formed as a by-product of NO(3)(−) assimilation. Additional experiments reveal NasT is required for NO(3)(−)-responsive expression of the narK-bjgb-flp-nasC transcriptional unit and the nirA gene and that NasS is also involved in the regulatory control of this novel bipartite assimilatory NO(3)(−)/NO(2)(−) reductase pathway
The endemic Chamaecytisus albidus is nodulated by symbiovar genistearum of Bradyrhizobium in the Moroccan Maamora Forest
Out of 54 isolates from root nodules of the Moroccan-endemic Chamaecytisus albidus plants growing in soils from the Maamora cork oak forest, 44 isolates formed nodules when used to infect their original host plant. A phenotypic analysis showed the metabolic diversity of the strains that used different carbohydrates and amino acids as sole carbon and nitrogen sources. The isolates grew on media with pH values ranging from 6 to 8. However, they did not tolerate high temperatures or drought and they did not grow on media with salt concentrations higher than 85 mM. REP-PCR fingerprinting grouped the strains into 12 clusters, of which representative strains were selected for ARDRA and rrs analyses. The rrs gene sequence analysis indicated that all 12 strains were members of the genus Bradyrhizobium and their phylogeny showed that they were grouped into two different clusters. Two strains from each group were selected for multilocus sequence analysis (MLSA) using atpD, recA, gyrB and glnII housekeeping genes. The inferred phylogenetic trees confirmed that the strains clustered into two divergent clusters. Strains CM55 and CM57 were affiliated to the B. canariense/B. lupini group, whereas strains CM61 and CM64 were regrouped within the B. cytisi/B. rifense lineage. The analysis of the nodC symbiotic gene affiliated the strains to the symbiovar genistearum. The strains were also able to nodulate Retama monosperma, Lupinus luteus and Cytisus monspessulanus, but not Phaseolus vulgaris or Glycine max. Inoculation tests with C. albidus showed that some strains could be exploited as efficient inocula that could be used to improve plant growth in the Maamora forest.The authors want to thank all the people who contributed to theachievement of this study. Financial support was obtained from‘‘Académie Hassan II des Sciences et Techniques” (Morocco). MrOmar Bouhnik received a grant from the Hassan II Academy of Science and Technology. The authors also thank the Ministerio de Economía, Industria y Competitividad (Spain) for the granting ofthe ERDF-cofinanced project AGL2017–85676R
The fodder legume Chamaecytisus albidus establishes functional symbiosis with different Bradyrhizobial symbiovars in Morocco
In this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains selected after REP-PCR fingerprinting showed that all the strains belong to the genus Bradyrhizobium. Following multi-locus sequence analysis (MLSA) using the rrs, gyrB, recA, glnII, and rpoB housekeeping genes, five representative strains, CA20, CA61, CJ2, CB10, and CB61 were selected for further molecular studies. Phylogenetic analysis of the concatenated glnII, gyrB, recA, and rpoB genes showed that the strain CJ2 isolated from Sahel Doukkala soil is close to Bradyrhizobium canariense BTA-1 (96.95%); that strains CA20 and CA61 isolated from the Amhach site are more related to Bradyrhizobium valentinum LmjM3, with 96.40 and 94.57% similarity values; and that the strains CB10 and CB60 isolated from soil in the Bounaga site are more related to Bradyrhizobium murdochi CNPSo 4020 and Bradyrhizobium. retamae Ro19, with which they showed 95.45 and 97.34% similarity values, respectively. The phylogenetic analysis of the symbiotic genes showed that the strains belong to symbiovars lupini, genistearum, and retamae. All the five strains are able to nodulate Lupinus luteus, Retama monosperma, and Cytisus monspessilanus, but they do not nodulate Glycine max and Phaseolus vulgaris. The inoculation tests showed that the strains isolated from the 3 regions improve significantly the plant yield as compared to uninoculated plants. However, the strains of Bradyrhizobium sp. sv. retamae isolated from the site of Amhach were the most performing. The phenotypic analysis showed that the strains are able to use a wide range of carbohydrates and amino acids as sole carbon and nitrogen source. The strains isolated from the arid areas of Bounaga and Amhach were more tolerant to salinity and drought stress than strains isolated in the semi-arid area of Sahel Doukkala.Financial support was obtained from Académie Hassan II des Sciences et Techniques (in Morocco). Mr Omar Bouhnik received a grant from the Hassan II Academy of Science and Technolog
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