Crónica de una costosa tragedia

Este libro constituye, quizá, el primer trabajo de investigación que estudia, desde una perspectiva histórica, la génesis de la destrucción y deterioro de un valiosísimo patrimonio natural como consecuencia de una obra construida por el hombre - una historia de errores forjada a lo largo de siglos. Al evidenciar y explicar el desastre ambiental generado por el Canal del Dique, José Vicente Mo-gollón marca un hito en la bibliografía sobre medio ambiente en el paísincluye referencias bibliográficas (página 248)

Infected pancreatic necrosis: outcomes and clinical predictors of mortality. A post hoc analysis of the MANCTRA-1 international study

: The identification of high-risk patients in the early stages of infected pancreatic necrosis (IPN) is critical, because it could help the clinicians to adopt more effective management strategies. We conducted a post hoc analysis of the MANCTRA-1 international study to assess the association between clinical risk factors and mortality among adult patients with IPN. Univariable and multivariable logistic regression models were used to identify prognostic factors of mortality. We identified 247 consecutive patients with IPN hospitalised between January 2019 and December 2020. History of uncontrolled arterial hypertension (p = 0.032; 95% CI 1.135-15.882; aOR 4.245), qSOFA (p = 0.005; 95% CI 1.359-5.879; aOR 2.828), renal failure (p = 0.022; 95% CI 1.138-5.442; aOR 2.489), and haemodynamic failure (p = 0.018; 95% CI 1.184-5.978; aOR 2.661), were identified as independent predictors of mortality in IPN patients. Cholangitis (p = 0.003; 95% CI 1.598-9.930; aOR 3.983), abdominal compartment syndrome (p = 0.032; 95% CI 1.090-6.967; aOR 2.735), and gastrointestinal/intra-abdominal bleeding (p = 0.009; 95% CI 1.286-5.712; aOR 2.710) were independently associated with the risk of mortality. Upfront open surgical necrosectomy was strongly associated with the risk of mortality (p < 0.001; 95% CI 1.912-7.442; aOR 3.772), whereas endoscopic drainage of pancreatic necrosis (p = 0.018; 95% CI 0.138-0.834; aOR 0.339) and enteral nutrition (p = 0.003; 95% CI 0.143-0.716; aOR 0.320) were found as protective factors. Organ failure, acute cholangitis, and upfront open surgical necrosectomy were the most significant predictors of mortality. Our study confirmed that, even in a subgroup of particularly ill patients such as those with IPN, upfront open surgery should be avoided as much as possible. Study protocol registered in ClinicalTrials.Gov (I.D. Number NCT04747990)

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

Destintado de papel de impresión láser mediante ultrasonido con dos frecuencias, 25 y 45 kHz

El objetivo de esta investigación fue analizar los efectos de un tratamiento de ultrasonido, combinado con las etapas de flotación y lavado, para mejorar la eficiencia del proceso de destintado del papel de desecho impreso con tecnología láser. El papel oficio de desperdicio fue cortado en piezas pequeñas de alrededor de 3 cm2, y posteriormente desintegrado en un dispositivo especial para obtener una suspensión base agua. Este proceso se hizo con una consistencia de 1.5 % por cinco minutos. Después de desintegrada, la suspensión de fibra se trató en un baño de ultrasonido con dos frecuencias, 25 y 45 kHz. Se tomaron datos a partir de un diseño experimental 25-1 y como factores se consideraron la frecuencia, consistencia, temperatura, el tiempo de tratamiento y el modo de la modulación de la frecuencia (barrido, desgasificación o sin modulación). El tamaño de la partícula y el número de partículas por metro cuadrado en cada etapa del proceso de destintado fueron evaluadas como variables de respuesta. Los resultados indican que la eficiencia en el rompimiento de las partículas de tinta depende de la frecuencia y del modo de modulación usado durante la fase de ultrasonido. El número máximo de partículas de tinta en el intervalo de 50-100 μm (tamaño óptimo por remoción de partícula) se obtuvo al usar la frecuencia de 25 kHz, 0.5 % de consistencia, modulación de 1 y tratamiento de 20 minutos de duración

Por D. Beatriz de Ouando, muger de don Iuan Roco Campofrio, Cauallero de la Orden de Alcantara. En el pleyto, con don Gabriel de Ouando Perero, y doña Maria de Oua¯do Bezerra, muger de D. Ioseph de Mayoralgo Enriquez, todos vezinos de la villa de Cazeres

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Mycosome-Coated Gold Nanoparticles for Plasmonic Detection of Tuberculosis-Associated Antibodies

Considering that more than 10 million people develop tuberculosis disease (TB) each year and that nearly 3 million of them are never diagnosed, label-free screening methods constitute one of the top public health priorities worldwide. Herein we report the fabrication of a plasmonic system for the detection of TB-associated antibodies. Gold nanoparticles (AuNPs) were synthesized at controlled sizes and modified to display antigen lipid glycans from mycobacteria, the group of organisms comprising the TB causal agent. Facile self-assembly procedures were employed to form mycobacterial phosphatidylinositol mannoside (PIM)-containing unilamellar liposomes (mycosomes) and to attach them onto gold surfaces using thiol chemistry. Using enzyme-linked immunoassays with rabbit hyperimmune sera, the constructs were found to specifically capture antilipid antibodies, presenting a dose-dependent response. Also, the functional hybrid nanoparticles showed localized surface plasmon resonance (LSPR) shifts of up to 2 nm upon their interaction with specific antibodies, indicating their suitability for the optical transduction of antigen–antibody reactions. Finally, the applicability of the system for real-world detection of TB-associated antibodies was demonstrated through analysis of 12 samples of human individuals, including 7 cases with pulmonary TB. To the best of our knowledge, this is the first biomimetic nanogold–lipid system for the implementation of lipids as immunosensing reagents for the plasmonic detection and diagnosis of TB