649 research outputs found
Integrating gene and protein expression data with genome-scale metabolic networks to infer functional pathways
This article has been made available through the Brunel Open Access Publishing Fund. Copyright @ 2013 Pey et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.Background: The study of cellular metabolism in the context of high-throughput -omics data has allowed us to decipher novel mechanisms of importance in biotechnology and health. To continue with this progress, it is essential to efficiently integrate experimental data into metabolic modeling. Results: We present here an in-silico framework to infer relevant metabolic pathways for a particular phenotype under study based on its gene/protein expression data. This framework is based on the Carbon Flux Path (CFP) approach, a mixed-integer linear program that expands classical path finding techniques by considering additional biophysical constraints. In particular, the objective function of the CFP approach is amended to account for gene/protein expression data and influence obtained paths. This approach is termed integrative Carbon Flux Path (iCFP). We show that gene/protein expression data also influences the stoichiometric balancing of CFPs, which provides a more accurate picture of active metabolic pathways. This is illustrated in both a theoretical and real scenario. Finally, we apply this approach to find novel pathways relevant in the regulation of acetate overflow metabolism in Escherichia coli. As a result, several targets which could be relevant for better understanding of the phenomenon leading to impaired acetate overflow are proposed. Conclusions:
A novel mathematical framework that determines functional pathways based on gene/protein expression data is presented and validated. We show that our approach is able to provide new insights into complex biological scenarios such as acetate overflow in Escherichia coli.Basque Governmen
Lessons from building an automated pre-departure sequencer for airports
Commercial airports are under increasing pressure to comply with the Eurocontrol collaborative decision making (CDM) initiative, to ensure that information is passed between stakeholders, integrate automated decision support or make predictions. These systems can also aid effective operations beyond the airport by communicating scheduling decisions to other relevant parties, such as Eurocontrol, for passing on to downstream airports and enabling overall airspace improvements. One of the major CDM components is aimed at producing the target take-off times and target startup-approval times, i.e. scheduling when the aircraft should push back from the gates and start their engines and when they will take off. For medium-sized airports, a common choice for this is a “pre-departure sequencer” (PDS). In this paper, we describe the design and requirements challenges which arose during our development of a PDS system for medium sized international airports. Firstly, the scheduling problem is highly dynamic and event driven. Secondly, it is important to end-users that the system be predictable and, as far as possible, transparent in its operation, with decisions that can be explained. Thirdly, users can override decisions, and this information has to be taken into account. Finally, it is important that the system is as fair as possible for all users of the airport, and the interpretation of this is considered here. Together, these factors have influenced the design of the PDS system which has been built to work within an existing large system which is being used at many airport
Feature Selection of Post-Graduation Income of College Students in the United States
This study investigated the most important attributes of the 6-year
post-graduation income of college graduates who used financial aid during their
time at college in the United States. The latest data released by the United
States Department of Education was used. Specifically, 1,429 cohorts of
graduates from three years (2001, 2003, and 2005) were included in the data
analysis. Three attribute selection methods, including filter methods, forward
selection, and Genetic Algorithm, were applied to the attribute selection from
30 relevant attributes. Five groups of machine learning algorithms were applied
to the dataset for classification using the best selected attribute subsets.
Based on our findings, we discuss the role of neighborhood professional degree
attainment, parental income, SAT scores, and family college education in
post-graduation incomes and the implications for social stratification.Comment: 14 pages, 6 tables, 3 figure
Crew Scheduling for Netherlands Railways: "destination: customer"
: In this paper we describe the use of a set covering model with additional constraints for scheduling train drivers and conductors for the Dutch railway operator NS Reizigers. The schedules were generated according to new rules originating from the project "Destination: Customer" ("Bestemming: Klant" in Dutch). This project is carried out by NS Reizigers in order to increase the quality and the punctuality of its train services. With respect to the scheduling of drivers and conductors, this project involves the generation of efficient and acceptable duties with a high robustness against the transfer of delays of trains. A key issue for the acceptability of the duties is the included amount of variation per duty. The applied set covering model is solved by dynamic column generation techniques, Lagrangean relaxation and powerful heuristics. The model and the solution techniques are part of the TURNI system, which is currently used by NS Reizigers for carrying out several analyses concerning the required capacities of the depots. The latter are strongly influenced by the new rules
Experiments on local search for bi-objective unconstrained binary quadratic programming
International audienceThis article reports an experimental analysis on stochastic local search for approximating the Pareto set of bi-objective unconstrained binary quadratic programming problems. First, we investigate two scalarizing strategies that iteratively identify a high-quality solution for a sequence of sub-problems. Each sub-problem is based on a static or adaptive definition of weighted-sum aggregation coefficients, and is addressed by means of a state-of-the-art single-objective tabu search procedure. Next, we design a Pareto local search that iteratively improves a set of solutions based on a neighborhood structure and on the Pareto dominance relation. At last, we hybridize both classes of algorithms by combining a scalarizing and a Pareto local search in a sequential way. A comprehensive experimental analysis reveals the high performance of the proposed approaches, which substantially improve upon previous best-known solutions. Moreover, the obtained results show the superiority of the hybrid algorithm over non-hybrid ones in terms of solution quality, while requiring a competitive computational cost. In addition, a number of structural properties of the problem instances allow us to explain the main difficulties that the different classes of local search algorithms have to face
Engineering Art Galleries
The Art Gallery Problem is one of the most well-known problems in
Computational Geometry, with a rich history in the study of algorithms,
complexity, and variants. Recently there has been a surge in experimental work
on the problem. In this survey, we describe this work, show the chronology of
developments, and compare current algorithms, including two unpublished
versions, in an exhaustive experiment. Furthermore, we show what core
algorithmic ingredients have led to recent successes
Yukawa hierarchies at the point of in F-theory
We analyse the structure of Yukawa couplings in local SU(5) F-theory models
with enhancement. In this setting the symmetry is broken down to
SU(5) by a 7-brane configuration described by T-branes, all the Yukawa
couplings are generated in the vicinity of a point and only one family of
quarks and leptons is massive at tree-level. The other two families obtain
their masses when non-perturbative effects are taken into account, being
hierarchically lighter than the third family. However, and contrary to previous
results, we find that this hierarchy of fermion masses is not always
appropriate to reproduce measured data. We find instead that different T-brane
configurations breaking to SU(5) give rise to distinct hierarchical
patterns for the holomorphic Yukawa couplings. Only some of these patterns
allow to fit the observed fermion masses with reasonable local model parameter
values, adding further constraints to the construction of F-theory GUTs. We
consider an model where such appropriate hierarchy is realised and
compute its physical Yukawas, showing that realistic charged fermions masses
can indeed be obtained in this case.Comment: 46 pages + appendices, 5 figures. v2, added references and typos
corrected, version accepted on JHEP. v3, typos correcte
Efficacy of olanzapine long-acting injection in patients with acutely exacerbated schizophrenia: an insight from effect size comparison with historical oral data
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