Archaeological supplement B to Damgaard et al. 2018: discussion of the archaeology of Central Asian and East Asian Neolithic to Bronze Age hunter-gatherers and early pastoralists, including consideration of horse domestication

This is the author accepted manuscript. The final version is available from Zenodo via the DOI in this recordThis is part of the additional supplementary material for Damgaard et al., The first horse herders and the impact of early Bronze Age steppe expansions into Asia. Science. DOI: 10.1126/science.aar7711. Note that the author accepted manuscript version of the article plus supplementary material and additional supplementary material is in ORE at http://hdl.handle.net/10871/32791The archaeological evidence relating to selected key cultures from Central and East Asia from the Neolithic to the Bronze Age is summarized. These cultures include the Eneolithic (Copper Age) Botai culture of northern Kazakhstan, the Bronze Age Okunevo culture from the Minusinsk Basin in Russia and Neolithic to Bronze Age cultures of the Baikal Region in East Siberia. Special consideration is given to the debate surrounding horse domestication within the Botai Culture, and the key lines of evidence are summarized

The first horse herders and the impact of early Bronze Age steppe expansions into Asia

This is the author accepted manuscript. The final version is available from AAAS via the DOI in this recordThe file includes the article, supplementary material and additional supplementary materialThe published version of the supplementary materials are at http://science.sciencemag.org/content/suppl/2018/05/08/science.aar7711.DC1Part of the additional supplementary materials for this article are in ORE at http://hdl.handle.net/10871/32792The Yamnaya expansions from the western steppe into Europe and Asia during the Early Bronze Age (~3000 BCE) are believed to have brought with them Indo-European languages and possibly horse husbandry. We analyze 74 ancient whole-genome sequences from across Inner Asia and Anatolia and show that the Botai people associated with the earliest horse husbandry derived from a hunter-gatherer population deeply diverged from the Yamnaya. Our results also suggest distinct migrations bringing West Eurasian ancestry into South Asia before and after but not at the time of Yamnaya culture. We find no evidence of steppe ancestry in Bronze Age Anatolia from when Indo-European languages are attested there. Thus, in contrast to Europe, Early Bronze Age Yamnaya-related migrations had limited direct genetic impact in Asia.The study was supported by the Lundbeck Foundation (EW), the Danish National Research Foundation (EW), and KU2016 (EW). Research at the Sanger Institute was supported by the Wellcome Trust (grant 206194). RM was supported by an EMBO Long-Term Fellowship (ALTF 133-2017). JK was supported by the Human Frontiers Science Program (LT000402/2017). Botai fieldwork was supported by University of Exeter, Archeology Exploration Fund and Niobe Thompson, Clearwater Documentary. AB was supported by NIH grant 5T32GM007197-43. GK was funded by Riksbankens Jubileumsfond and European Research Council. MP was funded by Netherlands Organization for Scientific Research (NWO), project number 276-70-028, IU was funded by the Higher education commission of Pakistan. Archaeological materials from Sholpan and Grigorievka were obtained with partial financial support of the budget program of the Ministry of Education and Science of the Republic of Kazakhstan “Grant financing of scientific research for 2018-2020” No. AP05133498 “Early Bronze Age of the Upper Irtysh”

Middle Holocene hunter–gatherers of Cis-Baikal, Eastern Siberia: chronology and dietary trends

Analyses of radiocarbon dates (all corrected for the freshwater reservoir effect) and associated stable isotope values obtained from the skeletal remains of ~650 individuals provide many new insights about Middle Holocene hunter–gatherers (HGs) of the Cis-Baikal region, Eastern Siberia. The new radiocarbon evidence clarifies the culture history of the region by defining better the boundaries between the chronological (archaeological periods) and cultural (mortuary traditions) units, as well as our understanding of the transitions between them. Furthermore, differences between the four archaeological micro-regions with regard to the timing and duration of these culture historical units have come into focus for the first time. In terms of dietary patterns, the Early Neolithic foragers of the Angara and Southwest Baikal trended towards a greater reliance on aquatic foods. A similar trend was found in the Late Neolithic (LN) Isakovo group on the Angara, while the LN Serovo group in the Little Sea trended towards an increased dietary reliance on terrestrial game. In the Early Bronze Age HGs, a mosaic of dietary patterns was found: some groups experienced dietary shifts (frequently emphasizing different foods), while other groups displayed stability. Such differences were found even between close neighbours. All these results suggest significant variation in patterns of culture change within and between archaeological periods, mortuary traditions, and micro-regions. Some cultural patterns developed at a quick pace, others much more slowly; some appear to have collapsed rapidly, while others probably went through a more gradual transition to a different pattern. Additionally, this large set of radiocarbon dates allows novel insights into patterns of cemetery use: some seem to have been used continuously, others only sporadically, and some show long periods of disuse. Moreover, some cemeteries of the same mortuary tradition were apparently in use substantially earlier than others were even established. In sum, Cis-Baikal Middle Holocene HG strategies underwent a range of changes not only at the boundaries between relevant culture historical units but also within such units. New insights suggest considerable spatio-temporal variation in the nature, pace, and timing of these developments

Spatio-temporal patterns of cemetery use among Middle Holocene hunter-gatherers of Cis-Baikal, Eastern Siberia

Hunter-gatherer archaeology typically focusses on the details of subsistence strategies and material culture and, in the case of cemeteries, on various aspects of mortuary practices, beliefs, and social differentiation. This paper aims to look rather at patterns of change over time and space in how past hunter-gatherer cemeteries were used from Late Mesolithic to Early Bronze Age (~8600–3500 cal. BP) in the Cis-Baikal region of Eastern Siberia. The approach is based on a Kernel Density methodology applied to 560 radiocarbon dates obtained for individual burials from 65 cemeteries and representing 5 distinct mortuary traditions. This enables a number of different types of analysis to be performed at different scales: (1) It is possible to examine the overall tempo of burial events at each cemetery or a group of cemeteries; (2) Within each cemetery the spatial patterns of the sequence of graves and burials can be analyzed further; (3) It is possible to compare the different cemetery-specific chronologies within the micro-region or regional context; and (4) Although tentatively at this time, the spatiotemporal pattern of cemetery use over the whole region and can be visualised. The spatio-temporal analysis of individual cemeteries shows that each one had its own pattern, some very distinct and clear in their characteristics, which relate to the role the cemetery played within the microregional or regional population. On the regional scale some broader patterns such as shifts in frequency of burial events between microregions within mortuary traditions are visible. However, at this scale the existing sampling biases require caution in assessment of the results and future fieldwork will help improve the analysis and insights. On the other hand, many of the individual cemeteries have been excavated in full and such comprehensive datasets already provide a range of entirely new and important insights into cemetery use by the Middle Holocene hunter-gatherers of Cis-Baikal

Middle Holocene hunter–gatherers of Cis-Baikal, Eastern Siberia: chronology and dietary trends

Analyses of radiocarbon dates (all corrected for the freshwater reservoir effect) and associated stable isotope values obtained from the skeletal remains of ~650 individuals provide many new insights about Middle Holocene hunter–gatherers (HGs) of the Cis-Baikal region, Eastern Siberia. The new radiocarbon evidence clarifies the culture history of the region by defining better the boundaries between the chronological (archaeological periods) and cultural (mortuary traditions) units, as well as our understanding of the transitions between them. Furthermore, differences between the four archaeological micro-regions with regard to the timing and duration of these culture historical units have come into focus for the first time. In terms of dietary patterns, the Early Neolithic foragers of the Angara and Southwest Baikal trended towards a greater reliance on aquatic foods. A similar trend was found in the Late Neolithic (LN) Isakovo group on the Angara, while the LN Serovo group in the Little Sea trended towards an increased dietary reliance on terrestrial game. In the Early Bronze Age HGs, a mosaic of dietary patterns was found: some groups experienced dietary shifts (frequently emphasizing different foods), while other groups displayed stability. Such differences were found even between close neighbours. All these results suggest significant variation in patterns of culture change within and between archaeological periods, mortuary traditions, and micro-regions. Some cultural patterns developed at a quick pace, others much more slowly; some appear to have collapsed rapidly, while others probably went through a more gradual transition to a different pattern. Additionally, this large set of radiocarbon dates allows novel insights into patterns of cemetery use: some seem to have been used continuously, others only sporadically, and some show long periods of disuse. Moreover, some cemeteries of the same mortuary tradition were apparently in use substantially earlier than others were even established. In sum, Cis-Baikal Middle Holocene HG strategies underwent a range of changes not only at the boundaries between relevant culture historical units but also within such units. New insights suggest considerable spatio-temporal variation in the nature, pace, and timing of these developments

Freshwater reservoir effects in Cis-Baikal: an overview

This paper summarises research on freshwater reservoir effects (FRE) in the Baikal region and their impact on the radiocarbon dating of human remains. Varying relationships are seen between human δ13C and δ15N values and 14C offsets in paired human-terrestrial mammal radiocarbon dates from the same graves in the different microregions of Cis-Baikal. In the Upper Lena microregion the FRE may also vary through time. These differences can be related in some cases to different isotopic ecologies, and in others to the presence of different old carbon reservoirs. Some areas requiring further research are highlighted, and the use of other proxies (δ2H, δ34S) for assessing the dietary contributions of freshwater resources is considered. A case study from the Early Neolithic cemetery of Shamanka II is used to illustrate the marked effects of changes in dietary catchment over an individual's life history, with bone and tooth dates from the same individual differing by 385 14C yr

Freshwater reservoir effects in Cis-Baikal: an overview

This paper summarises research on freshwater reservoir effects (FRE) in the Baikal region and their impact on the radiocarbon dating of human remains. Varying relationships are seen between human δ13C and δ15N values and 14C offsets in paired human-terrestrial mammal radiocarbon dates from the same graves in the different microregions of Cis-Baikal. In the Upper Lena microregion the FRE may also vary through time. These differences can be related in some cases to different isotopic ecologies, and in others to the presence of different old carbon reservoirs. Some areas requiring further research are highlighted, and the use of other proxies (δ2H, δ34S) for assessing the dietary contributions of freshwater resources is considered. A case study from the Early Neolithic cemetery of Shamanka II is used to illustrate the marked effects of changes in dietary catchment over an individual's life history, with bone and tooth dates from the same individual differing by 385 14C yr

Chronology of middle Holocene hunter-gatherers in the Cis-Baikal region of Siberia: Corrections based on examination of the freshwater reservoir effect

A dataset of 256 AMS radiocarbon dates on human skeletal remains from middle Holocene cemeteries in the Cis-Baikal region, Siberia, and associated carbon and nitrogen stable isotope values are analyzed for new insights about culture history and processes of culture change. First, based on the typological criteria all dated human burials are assigned to mortuary traditions and typochronological units-Late Mesolithic, Early Neolithic, Late Neolithic and Early Bronze Age. Next, all dates are corrected for the Freshwater Reservoir Effect (FRE) according to the regression equations developed using paired radiocarbon dates on human and terrestrial faunal remains from the same graves and examined for chronological trends using a Bayesian approach. While the entire corrected culture historical sequence is younger by roughly 200-400 years relative to the previous model the shift of the specific period boundaries is not systematic due to the varying proportion of aquatic food in the diets of the relevant groups. Examination of the dataset subdivided into smaller spatio-temporal units provides additional insights. During the Early Neolithic, in the Angara and Southwest Baikal micro-regions there is a chronological trend toward increased reliance on aquatic food. During the Early Bronze Age in the Little Sea micro-region, there appears to be a trend toward increased reliance on the Baikal seal. This shift, however, can also be interpreted as increasing migration over time of new groups from the Upper Lena. The sample from the Early Neolithic Shamanka II cemetery in Southwest Baikal shows two non-abutting phases of use each displaying a trend toward greater consumption of aquatic foods. These findings provide new chronological framework for the study of other cultural changes affecting middle Holocene hunter-gatherers in the region. The results may also allow better correlation with other sequences, cultural and environmental, that are not affected by the FRE

Using δ 2 H in human bone collagen to correct for freshwater 14 C reservoir offsets: a pilot study from Shamanka II, Lake Baikal, southern Siberia

There is increasing awareness of the need to correct for freshwater as well as marine reservoir effects when undertaking radiocarbon (14C) dating of human remains. Here, we explore the use of stable hydrogen isotopes (δ2H), alongside the more commonly used stable carbon (δ13C) and nitrogen isotopes (δ15N), for correcting 14C freshwater reservoir offsets in 10 paired human-faunal dates from graves at the prehistoric cemetery of Shamanka II, Lake Baikal, southern Siberia. Excluding one individual showing no offset, the average human-faunal offset was 515±175 14C yr. Linear regression models demonstrate a strong positive correlation between δ15N and δ2H ratios, supporting the use of δ2H as a proxy for trophic level. Both isotopes show moderate but significant correlations (r2 ~ 0.45, p < 0.05) with 14C offsets (while δ13C on its own does not), though δ2H performs marginally better. A regression model using all three stable isotopes to predict 14C offsets accounts for approximately 65% of the variation in the latter (r2=0.651, p=0.025), with both δ13C and δ2H, but not δ15N, contributing significantly. The results suggest that δ2H may be a useful proxy for freshwater reservoir corrections, though further work is needed