Bronchial-pulmonary adenocarcinoma subtyping relates with different molecular pathways

Lung cancer is one of the most common cancers in the world with a high mortality rate. We analyzed 45 surgical samples of the adenocarcinoma, 13 with lymph node metastasis. APC, BCL2, chromogranin A, CK 5/6/18 (LP34), CK20, CK7, cyclin D1, EGFR, ERCC1, HER2, Ki67, LRP, MRP, P53, RB and TTF1 expressions were evaluated by immunohistochemistry (IHC). Higher Ki67, APC, ERCC1 expressions and lower TTF1 expression were identified in advanced stages (IIA and IIIA) of adenocarcinomas, which reflect a more aggressive, less differentiated, possibly a non-TRU adenocarcinoma. Acinar, micropapillary and BA/lepidic adenocarcinoma patterns were the most similar patterns and papillary was the most different pattern followed by solid pattern, according to expression of these markers. Different adenocarcinoma patterns are engaged with different molecular pathways for carcinogenesis, based on the differences of expression. Acinar, BA/lepidic and micropapillary showed higher TTF1 expression (type TRU), and papillary and solid patterns revealed less TTF1 expression, exhibiting a non-TRU/bronchial phenotype. Solid pattern revealed lower HER2 and higher EGFR and ERCC1 (this compared to papillary) expression; papillary higher HER2 and lower ERCC1 expressions; micropapillary higher RB expression; and acinar lower ERCC1 and higher EGFR expressions. Ciclin D1 seems to have more importance in acinar and BA/lepidic patterns than in micropapillary. ERCC1 protein expression in micropapillary, solid and BA/lepidic patterns may indicate DNA repair activation. Inhibition of apoptosis could be explained by BCL2 overexpression, present in all adenocarcinoma patterns. MRP-1 and LRP were overexpressed in all patterns, which may have implications for drug resistance. Further studies are needed to interpret these data regarding to therapy response in advanced staged bronchial-pulmonary carcinomas.info:eu-repo/semantics/publishedVersio

Reflectance Confocal Microscopy for the Diagnosis of Galli-Galli Disease

info:eu-repo/semantics/publishedVersio

Consumo relativo de água em cultivares de melancia sob irrigação por gotejamento.

O estudo foi conduzido na Embrapa Meio-Norte, Teresina, PI (05°05? S; 42°48?W e 74,4m), objetivando determinar os valores de índice de satisfação da necessidade de água (ISNA), obtido pela relação ETr/ETc. Aplicaram-se, por meio de um sistema de gotejamento, quatro lâminas de irrigação diária equivalente a 50%; 75%; 100% e 125% de reposição da ET0, em um delineamento de blocos ao acaso, com parcelas subdivididas em faixas, com quatro repetições. A ETr e a ETc foram obtidas por meio do balanço hídrico no solo. Admitiu-se como ETc, a ETr oriunda do balanço hídrico na lâmina de irrigação de 125% da ETo. Foram utilizadas três cultivares de melancia: o híbrido duplo Top Gun, o híbrido triplo Shadow e a variedade Crimson Sweet, sob diferentes regimes hídricos. Para o híbrido duplo Top Gun, houve tendência de aumento dos valores de ISNA e da produtividade de frutos à medida que os níveis de disponibilidade hídrica no solo aumentaram, nas diferentes fases fenológicas da cultura, segundo um modelo linear. Para o híbrido triplo Shadow e a cultivar Crimson Sweet, o ISNA apresentou comportamento linear em função das lâminas de irrigação aplicadas, embora a produtividade de frutos tenha apresentado comportamento quadrático

a consensus statement from the Portuguese Pulmonology Society

Coronavirus disease 2019 (COVID-19) is an emerging infectious disease caused by a novel SARS-CoV-2 pathogen. Its capacity for human-to-human transmission through respiratory droplets, coupled with a high-level of population mobility, has resulted in a rapid dissemination worldwide. Healthcare workers have been particularly exposed to the risk of infection and represent a significant proportion of COVID-19 cases in the worst affected regions of Europe. Like other open airway procedures or aerosol-generating procedures, bronchoscopy poses a significant risk of spreading contaminated droplets, and medical workers must adapt the procedures to ensure safety of both patients and staff. Several recommendation documents were published at the beginning of the pandemic, but as the situation evolves, our thoughts should not only focus on the present, but should also reflect on how we are going to deal with the presence of the virus in the community until there is a vaccine or specific treatment available. It is in this sense that this document aims to guide interventional pulmonology throughout this period, providing a set of recommendations on how to perform bronchoscopy or pleural procedures safely and efficiently.proofpublishe

Recurrent patterns of phylogenetic habitat filtering in woody plant communities across phytogeographically distinct grassland-forest ecotones

The phylogenetic relationship among species may influence the mechanisms controlling local community assembly in ecological time. We analyzed the degree of recurrence of phylogenetic structure patterns in woody plant communities distributed along grassland-forest ecotones, across different vegetation types in southern Brazil, and the effect of phylogenetic pool size used to assess such patterns. Species frequency in quadrats distributed along grassland-forest ecotones was surveyed in different phytogeographic regions, where forests tend to expand over grasslands. We used principal coordinates of phylogenetic structure (PCPS) to evaluate the structure within vegetation quadrats divided into three habitat categories: grassland, forest edge and forest interior. Furthermore, phylogenetic structure measures were computed using different phylogenetic pool sizes. Our analyses showed consistent patterns in relation to habitat categories and to different phylogenetic pool sizes. Basal clades of angiosperms were associated with forest areas, while late-divergence clades were associated with grasslands. These results suggest that grasslands act as phylogenetic habitat filters to forest woody species, independently of species composition at each site and the phylogenetic pool. Rosanae and Asteranae act as vanguards of forest expansion over grasslands, while Magnolianae species tend to be restricted to forest. Our results shed light on the organization of ecological systems, providing evidence of recurrent phylogenetic structure patterns in ecotone plant communities at regional scale

Coating of Magnetite Nanoparticles with Fucoidan to Enhance Magnetic Hyperthermia Efficiency

Magnetic nanoparticles (NP), such as magnetite, have been the subject of research for application in the biomedical field, especially in Magnetic Hyperthermia Therapy (MHT), a promising technique for cancer therapy. NP are often coated with different compounds such as natural or synthetic polymers to protect them from oxidation and enhance their colloidal electrostatic stability while maintaining their thermal efficiency. In this work, the synthesis and characterization of magnetite nanoparticles coated with fucoidan, a biopolymer with recognized biocompatibility and antitumoral activity, is reported. The potential application of NP in MHT was evaluated through the assessment of Specific Loss Power (SLP) under an electromagnetic field amplitude of 14.7 kA m−1 and at 276 kHz. For fucoidan-coated NP, it was obtained SLP values of 100 and 156 W/g, corresponding to an Intrinsic Loss Power (ILP) of 1.7 and 2.6 nHm2kg−1, respectively. These values are, in general, higher than the ones reported in the literature for non-coated magnetite NP or coated with other polymers. Furthermore, in vitro assays showed that fucoidan and fucoidan-coated NP are biocompatible. The particle size (between ca. 6 to 12 nm), heating efficiency, and biocompatibility of fucoidan-coated magnetite NP meet the required criteria for MHT application

Noncommutative effects in astrophysical objects: a survey

The main implications of noncommutativity over astrophysical objects are examined. Noncommutativity is introduced through a deformed dispersion relation $E^{2}=p^{2}c^{2}(1+\lambda E)^{2} + m^{2}c^{4}$ and the relevant thermodynamical quantities are calculated using the grand canonical ensemble formalism. These results are applied to simple physical models describing main-sequence stars, white-dwarfs and neutron stars. The stability of main-sequence stars and white dwarfs is discussed.Comment: 10 pages. Talk presented by C. Z. at the "First Mediterranean Conference on Classical and Quantum Gravity", Kolymbari (Crete, Greece), September 14-18, 2009. To appear in the Proceeding