Radiation Information from 1958 δ2

The telemetered radiation information from the satellite 1958 δ2 (Sputnik III) has been analyzed for sixty-two separate passes recorded in College, Alaska. The data indicate a dependence of radiation intensity on altitude in the range 250-500 km. Both the high and low energy components apparently contribute to the overall increase of intensity with altitude, but the presence of a continuous afterglow in the scintillating crystal prevented detailed interpretation of the results.IGY Project No. 32.42 NSF Grant No. Y/32.42/268Ye

Estimated absorption of 136 mc/s satellite radio signals Interim technical report no. 1

Auroral, polar cap, and sudden cosmic noise absorption estimates for 136 mc/s satellite radio signa

Theory of phase-locking in generalized hybrid Josephson junction arrays

A recently proposed scheme for the analytical treatment of the dynamics of two-dimensional hybrid Josephson junction arrays is extended to a class of generalized hybrid arrays with ''horizontal'' shunts involving a capacitive as well as an inductive component. This class of arrays is of special interest, because the internal cell coupling has been shown numerically to favor in-phase synchronization for certain parameter values. As a result, we derive limits on the circuit design parameters for realizing this state. In addition, we obtain formulas for the flux-dependent frequency including flux-induced switching processes between the in-phase and anti-phase oscillation regime. The treatment covers unloaded arrays as well as arrays shunted via an external load.Comment: 24 pages, REVTeX, 5 Postscript figures, Subm. to Phys. Rev.

VP24-Karyopherin alpha binding affinities differ between Ebolavirus species, nfluencing interferon inhibition and VP24 stability

Zaire ebolavirus (EBOV), Bundibugyo ebolavirus (BDBV), and Reston ebolavirus (RESTV) belong to the same genus but exhibit different virulence properties. VP24 protein, a structural protein present in all family members, blocks interferon (IFN) signaling and likely contributes to virulence. Inhibition of IFN signaling by EBOV VP24 (eVP24) involves its interaction with the NPI-1 subfamily of karyopherin alpha (KPNA) nuclear transporters. Here, we evaluated eVP24, BDBV VP24 (bVP24), and RESTV VP24 (rVP24) interactions with three NPI-1 subfamily KPNAs (KPNA1, KPNA5, and KPNA6). Using purified proteins, we demonstrated that each VP24 binds to each of the three NPI-1 KPNAs. bVP24, however, exhibited approximately 10-fold-lower KPNA binding affinity than either eVP24 or rVP24. Cell-based assays also indicate that bVP24 exhibits decreased KPNA interaction, decreased suppression of IFN induced gene expression, and a decreased half-life in transfected cells compared to eVP24 or rVP24. Amino acid sequence alignments between bVP24 and eVP24 also identified residues within and surrounding the previously defined eVP24-KPNA5 binding interface that decrease eVP24-KPNA affinity or bVP24-KPNA affinity. VP24 mutations that lead to reduced KPNA binding affinity also decrease IFN inhibition and shorten VP24 half-lives. These data identify novel functional differences in VP24-KPNA interaction and reveal a novel impact of the VP24-KPNA interaction on VP24 stability. IMPORTANCE The interaction of Ebola virus (EBOV) VP24 protein with host karyopherin alpha (KPNA) proteins blocks type I interferon (IFN) signaling, which is a central component of the host innate immune response to viral infection. Here, we quantitatively compared the interactions of VP24 proteins from EBOV and two members of the Ebolavirus genus, Bundibugyo virus (BDBV) and Reston virus (RESTV). The data reveal lower binding affinity of the BDBV VP24 (bVP24) for KPNAs and demonstrate that the interaction with KPNA modulates inhibition of IFN signaling and VP24 stability. The effect of KPNA interaction on VP24 stability is a novel functional consequence of this virus-host interaction, and the differences identified between viral species may contribute to differences in pathogenesis

Instabilities in Josephson Ladders with Current Induced Magnetic Fields

We report on a theoretical analysis, consisting of both numerical and analytic work, of the stability of synchronization of a ladder array of Josephson junctions under the influence of current induced magnetic fields. Surprisingly, we find that as the ratio of the mutual to self inductance of the cells of the array is increased a region of unstable behavior occurs followed by reentrant stable synchronization. Analytic work tells us that in order to understand fully the cause of the observed instabilities the behavior of the vertical junctions, sometimes ignored in analytic analyses of ladder arrays, must be taken into account.Comment: RevTeX, 4 pages, 3 figure

Deriving Boltzmann Equations from Kadanoff-Baym Equations in Curved Space-Time

To calculate the baryon asymmetry in the baryogenesis via leptogenesis scenario one usually uses Boltzmann equations with transition amplitudes computed in vacuum. However, the hot and dense medium and, potentially, the expansion of the universe can affect the collision terms and hence the generated asymmetry. In this paper we derive the Boltzmann equation in the curved space-time from (first-principle) Kadanoff-Baym equations. As one expects from general considerations, the derived equations are covariant generalizations of the corresponding equations in Minkowski space-time. We find that, after the necessary approximations have been performed, only the left-hand side of the Boltzmann equation depends on the space-time metric. The amplitudes in the collision term on the right--hand side are independent of the metric, which justifies earlier calculations where this has been assumed implicitly. At tree level, the matrix elements coincide with those computed in vacuum. However, the loop contributions involve additional integrals over the the distribution function.Comment: 14 pages, 5 figures, extended discussion of the constraint equations and the solution for the spectral functio

Label-Free 3D Imaging of Development of Cell Patterns in Drosophila melanogaster Wing Imaginal Disc

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7-August 11, 201

Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex

Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of the T6SS, TssK, using the antibacterial T6SS of Serratia marcescens as a model system. TssK was confirmed to be essential for effector secretion by the T6SS. The native protein, although not an integral membrane protein, appeared to localize to the inner membrane, consistent with its presence within a membrane-anchored assembly. Recombinant TssK purified from S. marcescens was found to exist in several stable oligomeric forms, namely trimer, hexamer and higher-order species. Native-level purification of TssK identified TssF and TssG as interacting proteins. TssF and TssG, conserved T6SS components of unknown function, were required for T6SS activity, but not for correct localization of TssK. A complex containing TssK, TssF and TssG was subsequently purified in vitro, confirming that these three proteins form a new subcomplex within the T6SS. Our findings provide new insight into the T6SS assembly, allowing us to propose a model whereby TssK recruits TssFG into the membrane-associated T6SS complex and different oligomeric states of TssK may contribute to the dynamic mechanism of the system