Factores asociados a la practica de lactancia materna exclusiva en puerperas de parto eutocico del hospital regional Hermilio Valdizan Medrano Huanuco 2019

Objetivo: Identificar los factores asociados a la práctica de lactancia materna exclusiva en puérperas de parto eutócico del Hospital Regional Hermilio Valdizan Medrano Huánuco 2019. Métodos y técnicas: Según el grado de profundidad y alcance de tiempo la investigación fue de nivel descriptivo simple debido a que se realizó la recolecto de datos en un solo momento, con el propósito de comprobar la hipótesis planteada, para lo cual se utilizó una guía de entrevista. Resultados: En base a los factores individuales asociados a la práctica de lactancia materna exclusiva en puérperas de parto eutócico se evidencio de mayor predominio, el 74,6 de usuarias no cambiara la idea de brindar abandonar lactancia materna en los primeros 6 meses. En base a los factores microambientes, se evidencio de mayor predominio, el 71,2% de usuarias no reciben apoyo de su pareja durante la lactancia materna. En base a los factores comunitarios se evidencio el 76,3% de las usuarias no tienen confianza con el personal de salud en el momento del puerperio. En el presente estudio de acuerdo a la hipótesis descriptiva al realizar las pruebas estadísticas por dimensiones estamos aceptando la hipótesis de investigación (1) en donde se plantea que existen factores individuales asociados a la práctica de lactancia materna exclusiva, también se acepta la hipótesis (2) en donde se plante que no existe la factores de riesgo individuales asociados a la práctica de lactancia materna exclusiva, también se acepta la hipótesis (3) en donde se plantea que si existe factores microambientes asociados a la práctica de lactancia materna exclusiva, asimismo se acepta la hipótesis (4) en donde se plante que no existe factores microambientes asociados a la práctica de lactancia materna exclusiva, de igual manera se acepta la hipótesis (5) en donde se plantea que existe factores de riesgo comunitario asociados a la práctica de lactancia materna exclusiva, de igual modo se acepta la hipótesis (6) en donde se plantea que no existe factores comunitarios asociados a la práctica de lactancia materna exclusiva. Conclusiones: Existen factores individuales, microambiente y comunitarios asociados a la práctica de lactancia materna exclusiva.Tesi

Biofunctionalization of cork with Moringa oleifera seeds and use of PMA staining and qPCR to detect viability of Escherichia coli

Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27−4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth

La imagen y La Narrativa Como Herramientas del Abordaje Psicosocial en Escenarios de Violencia. Cartagena Departamento de Bolívar

En este informe, presentamos un análisis de los eventos psicosociales y traumáticos desde una perspectiva psicológica, elaborando análisis y dando profundidad a los contenidos estudiados. Inicialmente se realiza análisis reflexivo sobre relato titulado “Carlo Arturo” tomado del libro: VOCES: Relatos de violencia y esperanza en Colombia, Editado por el Banco mundial en el año 2009, tomando como base teórica el enfoque narrativo, se construyen preguntas circulares, reflexivas y estratégicas con las cuales, nos acercarnos a una propuesta de intervención psicosocial para este caso. Posteriormente se realiza análisis del caso de las comunidades de Cacarica, en donde se presentan los emergentes psicosociales latentes, los impactos generados por la estigmatización, la propuesta de dos acciones de apoyo y tres estrategias de acompañamiento psicosociales. Y por último se presenta análisis reflexivo de la experiencia con la aplicación de la herramienta de acción psicosocial foto voz, en donde se identifican las convergencias y aspectos emergentes en de las narrativas visuales, que nos permitieron ampliar nuestro marco comprensivo de los fenómenos sociales e igualmente se construye blog en la plataforma wix.com, creando el salón de exposiciones titulado “Las verdades ocultas de la realidad social de víctimas de las diferentes formas de violencia en el departamento de Bolívar”. El desarrollo del diplomado en “acompañamiento psicosocial en escenarios de violencia” nos brindó a los participantes las herramientas teóricas y metodológicas para el acompañamiento psicosocial en situaciones traumáticas, crisis y violencias a las que se ve expuesta una persona, grupo, institución y/o comunidad.In this report, we present an analysis of psychosocial and traumatic events from a psychological perspective, developing analyses and giving depth to the contents studied. Initially, thoughtful analysis of the story entitled "Carlo Arturo" takes from the book: VOCES: Stories of Violence and Hope in Colombia, edited by the World Bank in 2009, based on the theoretical basis of the narrative approach, circular, reflective and strategic questions are built with which, approaching a proposal for psychosocial intervention for this case. Subsequently, analysis of the communities of Cacarica, where the emerging latent psychosocial are presented, the impacts generated by stigmatization, the proposal of two support actions and three psychosocial accompaniment strategies. And finally, it presents thoughtful analysis of the experience with the application of the psychosocial action tool photo voice, which identifies the convergences and emerging aspects in visual narratives, which allowed us to expand our comprehensive framework of social phenomena and also build blogs on the platform wix.com, creating the exhibition hall entitled "The hidden truths of the social reality of victims of the different forms of violence in the department of Bolivar". The development of the diploma in "psychosocial accompaniment in situations of violence" gave participants the theoretical and methodological tools for psychosocial accompaniment in traumatic situations, crises and violence to which a person, group, institution and / or community is exposed

The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells

Abstract Background Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. Main body of the abstract The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells. Conclusions This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express. Graphical Abstrac

Correction to: Autolysis of Pichia pastoris induced by cold

The original version of this article (Bartolo-Aguilar et al. 2017) was written and published including the first construction strategy of pLGC09, but not the final one. This error was pointed out by a reader and an analysis of sequences of parts of the plasmid corroborated this. The final construction strategy was reanalysed and confirmed the error. This error affected the text, Table 2, Fig. 1 and Additional files, but did not affect the results and conclusions stated in the paper. The authors regret that this error occurred in the original publication of the article. The corrected text, Table 2 and Fig. 1, and Additional files (Additional file 1. Construction strategy of pLGC09 and Additional file 2. Plasmid pLGC09) are given in this correction

Autolysis of Pichia pastoris induced by cold

Abstract The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae. In the transgenic P. pastoris, the expression of eng increased 3.6-fold after chilling the cells from 30 to 4 °C (cold-shock stage) followed by incubation for 6 h (eng expression stage). The culture was heated to 30 °C for 6 h (ENG synthesis stage) and kept at 37 °C for 24 h (lysis stage). After this procedure the cell morphology changed, spheroplasts were obtained and cellular lysis was observed. Thus, a clone of P. pastoris was obtained, which undergoes autolysis after a cold-shock

MOESM1 of Autolysis of Pichia pastoris induced by cold

Additional file 1. Construction strategy of pLGC09

The Extracellular Vesicles from the Commensal Staphylococcus Epidermidis ATCC12228 Strain Regulate Skin Inflammation in the Imiquimod-Induced Psoriasis Murine Model

Extracellular vesicles (EVs) are evaginations of the cytoplasmic membrane, containing nucleic acids, proteins, lipids, enzymes, and toxins. EVs participate in various bacterial physiological processes. Staphylococcus epidermidis interacts and communicates with the host skin. S. epidermidis’ EVs may have an essential role in this communication mechanism, modulating the immunological environment. This work aimed to evaluate if S. epidermidis’ EVs can modulate cytokine production by keratinocytes in vitro and in vivo using the imiquimod-induced psoriasis murine model. S. epidermidis’ EVs were obtained from a commensal strain (ATC12228EVs) and a clinical isolated strain (983EVs). EVs from both origins induced IL-6 expression in HaCaT keratinocyte cultures; nevertheless, 983EVs promoted a higher expression of the pro-inflammatory cytokines VEGF-A, LL37, IL-8, and IL-17F than ATCC12228EVs. Moreover, in vivo imiquimod-induced psoriatic skin treated with ATCC12228EVs reduced the characteristic psoriatic skin features, such as acanthosis and cellular infiltrate, as well as VEGF-A, IL-6, KC, IL-23, IL-17F, IL-36γ, and IL-36R expression in a more efficient manner than 983EVs; however, in contrast, Foxp3 expression did not significantly change, and IL-36 receptor antagonist (IL-36Ra) was found to be increased. Our findings showed a distinctive immunological profile induction that is dependent on the clinical or commensal EV origin in a mice model of skin-like psoriasis. Characteristically, proteomics analysis showed differences in the EVs protein content, dependent on origin of the isolated EVs. Specifically, in ATCC12228EVs, we found the proteins glutamate dehydrogenase, ornithine carbamoyltransferase, arginine deiminase, carbamate kinase, catalase, superoxide dismutase, phenol-soluble β1/β2 modulin, and polyglycerol phosphate α-glucosyltransferase, which could be involved in the reduction of lesions in the murine imiquimod-induced psoriasis skin. Our results show that the commensal ATCC12228EVs have a greater protective/attenuating effect on the murine imiquimod-induced psoriasis by inducing IL-36Ra expression in comparison with EVs from a clinical isolate of S. epidermidis