Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII

A 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII

The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames

We report the sequence of a 9000 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete previously unknown open reading frames, which were named G7587, G7589, G7591 and G7594 following standard rules for provisional nomenclature. Outstanding features of some of these proteins were the homology of the putative protein coded by G7589 with proteins involved in transcription regulation and the transmembrane domains predicted in the putative protein coded by G7591

Platelet function, coagulation and fibrinolysis in patients with previous coronary and cerebrovascular ischemic events

OBJECTIVES: Ischemic stroke (IS) or transient ischemic attack (TIA) history is present in 4-17% of patients with coronary artery disease (CAD). This subgroup of patients is at high risk for both ischemic and bleeding events. The aim of this study was to determine the role of platelet aggregability, coagulation and endogenous fibrinolysis in patients with CAD and previous IS or TIA. METHODS: A prospective case-control study that included 140 stable CAD patients divided into two groups: the CASE group (those with a previous IS/TIA, n=70) and the CONTROL group (those without a previous IS/TIA, n=70). Platelet aggregability (VerifyNow Aspirins and VerifyNow P2Y12s), coagulation (fibrinogen and thromboelastography by Reoroxs) and endogenous fibrinolysis (D dimer and plasminogen activator inhibitor-1) were evaluated. RESULTS: Patients in the CASE group presented significantly higher systolic blood pressure levels (135.84±16.09 vs 123.68±16.11, po0.01), significantly more previous CABG (25.71% vs 10%, p=0.015) and significantly higher calcium channel blocker usage (42.86% vs 24.29%, p=0.02) than those in the control group. In the adjusted models, low triglyceride values, low hemoglobin values and higher systolic blood pressure were significantly associated with previous IS/TIA (CASE group). Most importantly, platelet aggregability, coagulation and fibrinolysis tests were not independently associated with previous cerebrovascular ischemic events (CASE group). CONCLUSION: Platelet aggregability, coagulation and endogenous fibrinolysis showed similar results among CAD patients with and without previous IS/TIA. Therefore, it remains necessary to identify other targets to explain the higher bleeding risk presented by these patients

Propostas de roteiros turísticos no território de Fátima

Trabalho de Projeto de Mestrado em História da Arte, apresentado à Faculdade de letras da Universidade de CoimbraO presente trabalho de projeto propõe-se tratar o património e a animação turística. Partindo do princípio que a animação turística tem um papel fulcral na divulgação do património promovendo a sua preservação, é elaborado no presente trabalho um estudo de caso sobre o território de Fátima, apresentandose posteriormente propostas de roteiros turísticos para este território, com o objetivo de dinamizar e preservar o património, bem como aumentar e diversificar a oferta turística no território de Fátima. No decorrer do trabalho de projeto é realizada uma contextualização teórica da relevância do património para a animação turística, em geral, e para a realização de roteiros turísticos, em particular. No seguimento disto, é efetuada uma análise da oferta de animação turística neste território, bem como uma caracterização de lugares que detêm bens com valor patrimonial relevante. O trabalho assenta na criação de quatro roteiros turístico-culturais e de um roteiro de turismo de natureza: Roteiro dos Castelos do Rio Tejo; Roteiro Monumental de Coimbra e Condeixa-a-Nova; Roteiro Gótico de Batalha e Alcobaça; Roteiro Industrial do Vidro da Marinha Grande e da Cerâmica das Caldas da Rainha e o Roteiro de Turismo de Natureza da Serra de Aire e Candeeiros, sendo apresentada uma ficha técnica para cada um.The following work project proposes to address the problem of heritage and touristic entertainment. Assuming that the touristic entertainment has a key role in disseminate the heritage, promoting at the same time its preservation, it is conducted in this academic work a case study on Fatima's area, for subsequently submit tourist routes proposals for this territory with the main aim of stimulate and preserve the heritage as well as increase and diversify the touristic offer in Fatima's area. In the course of the project work is carried out a theorical contextualization about the relevance of heritage for touristic entertainment, in general, and for implementation of touristic routes, in particular. Following this, an analysis is made about the touristic entertainement offer in this territory, as well characterization of places that hold assets with heritage value. The work is based on the creation of four cultural routes: Castles of Tejo River Route; Coimbra and Condeixa-a-nova Monumental Route; Alcobaça and Batalha Gothic Route; Ceramic of Caldas da Rainha and Glass of Marinha Grande Industry Route and a Nature tourism route: Serra de Aire e Candeeiros Nature Tourism Route, being presented with a technical sheet for each one

Experiência perceptiva e o sentido da vida em psicólogos voluntários que atendem por telepsicologia no contexto da pandemia de Covid-19: um estudo em Viktor Frankl

Em meio ao surgimento da pandemia de Covid-19 a psicologia precisou se adequar, prestando atendimento através de Telepsicologia. Vários profissionais uniram-se para prestar atendimento voluntariamente e desafios precisaram ser superados. A construção deste projeto deu-se a partir de pesquisa, análise e coleta de informações e percepções de psicólogos voluntários que atenderam de maneira virtual a população durante a pandemia. Objetivo geral: desvelar compreensivamente a percepção e o sentido da vida para psicólogos voluntários que atuaram por meio da Telepsicologia no contexto da pandemia de Covid-19. Objetivos específicos: descrever a experiência perceptiva de psicólogos voluntários que atenderam pela Telepsicologia no contexto da pandemia de Covid-19. Identificar qual o sentido da vida para esses profissionais e quais as estratégias de enfrentamento utilizadas no cuidado de si. Método: Trata-se de um estudo descritivo com abordagem qualitativa e respaldo da fenomenologia existencial de Merleau-Ponty. O marco filosófico, conceitual e teórico usado é o da Logoterapia de Viktor Frankl. Após a aprovação do projeto pelo Comitê de Ética em Pesquisa (CEP) e com publicação do parecer consubstanciado, CAAE: 39944620.8.0000.5243, iniciou-se o estudo. Foi empregado um questionário semi estruturado e auto aplicável, repassado através de e-mail e pela mídia social WhatsApp, utilizando-se da técnica de snowball. A coleta dos dados ocorreu entre os meses de abril e junho de 2021, encerrando-se quando da percepção de saturação dos dados. As questões levantadas abrangeram os seguintes aspectos: conhecimento e preparo para atender de forma remota, motivação e o sentido dado a experiência de atendimento voluntário e o cuidado de si e a espiritualidade. O cenário da pesquisa foi totalmente virtual. Resultados: Foram identificadas três categorias: Conhecimento e preparo para atender pela telepsicologia; Percepção e sentido da vida através do altruísmo e vivência de dificuldades e Estratégias de enfrentamento e a espiritualidade no trabalho voluntário. Doar-se como voluntário é dar uma resposta a um chamado interior, independente de religião. Prestar atendimento psicológico é ter, acima de tudo, uma postura diante da vida, em que apesar das dificuldades, consegue-se elaborar as dores e construir estratégias de superação utilizando-se da espiritualidade como fonte de auxílio. Conclusão: Os resultados validam as questões da pesquisa, mostrando que o voluntário apreende o significado da conjuntura vivida e consegue extrair o sentido do que representa o ato de acolher o outro. A espiritualidade foi apontada como fundamental para o bem estar existencial e, com seu auxílio, o profissional encontra um sentido para sua vida, fato que o impulsiona a continuar em seu trabalho. A identificação de certas dificuldades técnicas permitiu desenvolver um produto que contemple checar a segurança dos atendimentos virtuais. Limitações: A coleta de dados foi realizada em um curto período. Observou-se uma evidente lacuna de conhecimento na literatura sobre o tema.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorAmid the outbreak of the Covid-19 pandemic, psychology had to adapt, providing care through Telepsychology. Several professionals got together to provide volunteer assistance and challenges had to be overcome. The construction of this project was based on research, analysis and collection of information and perceptions of volunteer psychologists who provided virtual services to the population during the pandemic. General objective: to comprehensively unveil the perception and meaning of life for volunteer psychologists who worked through Telepsychology in the context of the Covid-19 pandemic. Specific Aims: To describe the perceptual experience of volunteer psychologists who worked with telepsychology in the context of the Covid-19 pandemic. To identify the meaning of life for these professionals and the coping strategies used in self-care. Method: This is a descriptive study with a qualitative approach and supported by Merleau-Ponty's existential phenomenology. The philosophical, conceptual and theoretical framework used is Viktor Frankl's Logotherapy. After the project was approved by the Research Ethics Committee (CEP) and with the publication of the consubstantiated opinion, CAAE: 39944620.8.0000.5243, the study began. A semi-structured and self-applicable questionnaire was used, sent by e-mail and by the social media WhatsApp, using the snowball technique. Data collection took place between April and June 2021, and ended when data saturation was reached. The questions covered the following aspects: knowledge and preparation to attend remotely, motivation and meaning given to the experience of volunteer service, and self-care and spirituality. The research scenario was entirely virtual. Results: Three categories were identified: Knowledge and preparation to attend by telepsychology; Perception and meaning of life through altruism and experience of difficulties and Coping strategies and spirituality in volunteer work. To donate yourself as a volunteer is to respond to an inner calling, regardless of religion Providing psychological care is, above all, having an attitude towards life, in which, despite the difficulties, it is possible to elaborate the pain and build strategies for overcoming them, using spirituality as a source of help. Conclusion: The results validate the research questions, showing that the volunteer grasps the meaning of the situation he goes through and is able to extract the meaning of what the act of welcoming the other represents. Spirituality was pointed out as fundamental to the existential well-being, and with its help, the professional finds meaning to his life, a fact that impels him to continue his work. The identification of certain technical difficulties allowed the development of a product that includes checking the safety of virtual assistance. Limitations: The data collection was entirely virtual and carried out in a period of severe measures of social isolation. An evident knowledge gap was observed in the literature on the subject.190 f

Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs.

A 17.6 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains twelve open reading frames (ORFs) longer than 100 amino acids. Three genes had already been cloned and sequenced: CCT, ADE3 and TR-I. Two ORFs are similar to other yeast genes: G7722 with the YAL023 (PMT2) and PMT1 genes, encoding two integral membrane proteins, and G7727 with the first half of the genes encoding elongation factors 1gamma, TEF3 and TEF4. Two other ORFs, G7742 and G7744, are most probably yeast orthologues of the human and Paracoccus denitrificans electron-transferring flavoproteins (beta chain) and of the Escherichia coli phosphoserine phosphohydrolase. The five remaining identified ORFs do not show detectable homology with other protein sequences deposited in data banks. The sequence has been deposited in the EMBL data library under Accession Number Z49133

A prática desportiva dos estudantes universitários e suas relações com as autopercepções físicas, bem-estar subjectivo e felicidade University students' sport practice and its relations with physical self-perceptions, subjective well-being and happiness

A presente investigação analisou as relações entre a prática desportiva de estudantes universitários e o modo como estes se percepcionavam a si próprios e às suas vidas. Participaram nesta investigação 2284 estudantes de ambos os sexos, com idades entre os 18 e os 30 anos (M = 21,87 ±2,37), que preencheram um questionário sobre a sua prática desportiva, bem como versões portuguesas do Physical Self-Perception Profile (Fox & Corbin, 1989), da Subjective Happiness Scale (Lyubomirsky & Lepper, 1999), da Satisfaction With Life Scale (Diener, Emmons, Larsen, & Griffin, 1985) e da Positive and Negative Affect Schedule (Watson, Clark, & Tellegen, 1988). Em geral, a análise dos resultados revelou que os indivíduos que possuíam mais confiança física e que avaliavam a sua vida mais positivamente praticavam desporto mais regularmente; porém, verificou-se igualmente que quem se percebia menos forte fisicamente e tinha uma auto-estima mais baixa também praticava desporto mais regularmente do que os pares.<br>The purpose of this study was to examine the relationships between university students' sport practice and the way they perceived themselves and their lives. Participated in this investigation 2284 male and female students, with ages between 18 and 30 years (M = 21.87 ±2.37), who filled out a questionnaire to assess their levels of sport practice, as well the Portuguese versions of the Physical Self-Perception Profile (Fox & Corbin, 1989), the Subjective Happiness Scale (Lyubomirsky & Lepper, 1999), the Satisfaction With Life Scale (Diener, Emmons, Larsen, & Griffin, 1985) and the Positive and Negative Affect Schedule (Watson, Clark, & Tellegen, 1988). Overall, the analysis of the results showed that students who were more physically confident and evaluated their lives more positively did sport more regularly; however, results also showed that students who reported lower levels of physical strength and self-esteem practiced sport more regularly than their peers