Hippo signalling governs cytosolic nucleic acid sensing through YAP/TAZ-mediated TBK1 blockade

The Hippo pathway senses cellular conditions and regulates YAP/TAZ to control cellular and tissue homeostasis, while TBK1 is central for cytosolic nucleic acid sensing and antiviral defence. The correlation between cellular nutrient/physical status and host antiviral defence is interesting but not well understood. Here we find that YAP/TAZ act as natural inhibitors of TBK1 and are vital for antiviral physiology. Independent of transcriptional regulation and through the transactivation domain, YAP/TAZ associate directly with TBK1 and abolish virus-induced TBK1 activation, by preventing TBK1 Lys63-linked ubiquitylation and the binding of adaptors/substrates. Accordingly, YAP/TAZ deletion/depletion or cellular conditions inactivating YAP/TAZ through Lats1/2 kinases relieve TBK1 suppression and boost antiviral responses, whereas expression of the transcriptionally inactive YAP dampens cytosolic RNA/DNA sensing and weakens the antiviral defence in cells and zebrafish. Thus, we describe a function of YAP/TAZ and the Hippo pathway in innate immunity, by linking cellular nutrient/physical status to antiviral host defence

Extension des modèles de Carlino et Mills en vue d'examiner les effets de la taille et de la croissance urbaine sur les espaces ruraux sous influence urbaine

International audienceUne extension du modèle de développement économique local de Boarnet (1994) est proposée. Elle permet de relier les évolutions de population et d'emploi de l'hinterland rural à la croissance urbaine des centres d'emploi correspondants. Le modèle à deux équations simultanées qui en résulte est estimé sur des données danoises (204 municipalités), françaises (3 515 communes) et américaines (268 census tracts de Caroline du Sud). Les résultats montrent que les effets de diffusion urbaine vers l'hinterland rural sont le plus souvent significatifs et tendent à dominer les effets de "backwash" urbain. En conséquence, les communautés rurales sont concernées par les modalités du développement économique des villes et par les politiques qui affectent les schémas de la croissance urbaine entre le centre des villes et leur périurbain

Research on Prediction Model of Interlayer Leakage in Separate Injection Wells in Offshore Oilfields

Abstract In order to ensure effective layered water injection, it is of great significance to establish the functional relationship among the equivalent diameter of damage, leakage and pressure of sealing cylinder. In this paper, the experimental and numerical simulation experiments were carried out to study the relationship between gap flow rate, pressure drop and equivalent diameter of sealing cylinder under the conditions of uniform corrosion, fracture non-uniformity and random groove non-uniform distribution and scratches in different tubing, equivalent diameters and gap widths. Through a lot of data analysis, the relationship between the damage state and the leakage quantity is established and evaluated by physical simulation test. The research shows that the error between the established model function relation and the test data is no more than 10%. By simulating the damage condition of the shock sealing cylinder and quantitatively detecting the defect of the sealing cylinder, the model function relation can well predict the corresponding damage situation. Therefore, this model function can be used to solve sealing cylinder inspection problem, so as to judge the seal defect and leakage quantity which is independent of the experience of the person in charge of the site and able to use the data as a basis for judgment. This research provides a new technology for the effective prediction of the damage of sealing cylinder, and provides theoretical guidance for selecting matching sealing tools and realizing layered mining

The Protective Effect of Bcl-xl Overexpression against Oxidative Stress-Induced Vascular Endothelial Cell Injury and the Role of the Akt/eNOS Pathway

Restenosis after intraluminal or open vascular reconstruction remains an important clinical problem. Vascular endothelial cell (EC) injury induced by oxidative stress plays an important role in the development of intimal hyperplasia. In this study, we sought to evaluate the protective effects of Bcl-xl overexpression in vitro on oxidative stress-induced EC injury and the role of the Akt/endothelial nitric oxide synthase (eNOS) pathway. Human umbilical vein endothelial cells (HUVECs) exposed to hydrogen peroxide (H2O2, 0.5 mM) were used as the experimental oxidative stress model. The Bcl-xl gene was transferred into HUVECs through recombinant adenovirus vector pAdxsi-GFP-Bcl-xl before oxidative treatment. Cell apoptosis was evaluated by Annexin V/propidium iodide and Hoechst staining, caspase-7 and PARP cleavage. Cell viability was assessed using the cell counting kit-8 assay, proliferating cell nuclear antigen (PCNA) immunocytochemical detection and the scratching assay. Expressions of Akt, phospho-Akt and eNOS were detected by Western blotting. Our results showed that H2O2 induced apoptosis and decreased the cell viability of HUVECs. Bcl-xl overexpression significantly protected cells from H2O2-induced cell damage and apoptosis and maintained the cell function. Furthermore, the level of phospho-Akt and eNOS protein expression was significantly elevated when pretreated with Bcl-xl gene transferring. These findings suggest that Bcl-xl overexpression exerts an anti-apoptotic and protective effect on EC function. The Akt/eNOS signaling pathway is probably involved in these processes

Diversity maintenance and use of Vicia faba L. genetic resources

The faba bean (Vicia faba L.) is an ancient crop that is represented in collections by cultivated forms only. Botanic and molecular data suggest that the wild ancestor of this species has not yet been discovered or has become extinct. This fact makes ex situ collections more crucial for the present and future breeding activities of this crop, especially when the modernization of agriculture reduces genetic diversity. At the world level, more than 38,000 accession entries are included in at least 37 listed collections. The partial allogamous status of this crop makes maintenance activity of genotypes more expensive and difficult. In addition to the requirement of fixed genotypes to support new association genetics strategies, there is a need for more research on pollinators and maintenance of genetic diversity within populations. A large genetic variability has already been identified in V. faba in terms of floral biology, seed size and composition, and also tolerance to several biotic and abiotic stresses. More knowledge is needed on the interactions of V. faba with parasitic and pollinator insects, on traits related to environmental adaptation and impacts, on nitrogen fixation in interaction with soil Rhizobia and on bioenergy potential, which strengthens the demand for new and large phenotyping actions. Diversity analysis through genotyping is just beginning. The use of amplified fragment length polymorphism (AFLP) or simple sequence repeat (SSR) markers has allowed genetic resources to be distinguished according to their geographic origin and the structuring of collections. Conservation of gene sequences among legume species and the rapid discovery of genes (particularly in the model species Medicago truncatula and Lotus japonicus), together with robotic developments in molecular biology, offer new possibilities for the analysis of sequence diversity for V. faba genes and to evaluate their impact on phenotypic traits. Combined genotyping and phenotyping projects must continue on V. faba so that core collections can be defined; these will help in the discovery of genes and alleles of interest for breeders. © 2008 Elsevier B.V. All rights reserved.The contribution of M.J. Suso was partially supported by the RF2006-00002-00-00 project.Peer Reviewe

High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety

Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population

The molecular gas resolved by ALMA in the low-metallicity dwarf merging galaxy Haro 11

The physical mechanisms for starburst or quenching in less massive ($M_* < 10^{10} M_{\odot}$) galaxies are unclear. The merger is one of the inescapable processes referred to as both starburst and quenching in massive galaxies. However, the effects of the merger on star formation in dwarf galaxies and their evolution results are still uncertain. We aim to explore how to trigger and quench star formation in dwarf galaxies by studying the metal-poor gas-rich dwarf mergers based on the multi-band observations at a spatial resolution of $\sim$ 460 pc. We use the archival data of ALMA (band 3, 8) and VLT/MUSE to map CO($J=$1-0), [CI]($^3$P$_1 - ^3$P$_0$), and H$\alpha$ emission in one of the most extreme starburst merging dwarf galaxies, Haro 11. We find the molecular gas is assembled around the central two star-forming regions. The molecular/ionized gas and stellar components show complex kinematics, indicating that the gas is probably at a combined stage of collision of clouds and feedback from star formation. The peak location and distribution of [CI](1-0) strongly resemble the CO(1-0) emission, meaning that it might trace the same molecular gas as CO in such a dwarf merger starburst galaxy. The enhancement of line ratios ($\sim 0.5$) of [CI]/CO around knot C is probably generated by the dissociation of CO molecules by cosmic rays and far-ultraviolet photons. Globally, Haro 11 and its star-forming regions share similar SFEs as the high-$z$ starburst galaxies or the clumps in nearby (U)LIRGs. Given the high SFE, sSFR, small stellar mass, low metallicity, and deficient HI gas, Haro 11 could be an analog of high-$z$ dwarf starburst and the potential progenitor of the nearby less massive elliptical galaxies. The significantly smaller turbulent pressure and viral parameter will probably trigger the intense starbursts. We also predict that it will quench at $M_* < 8.5 \times 10^9 M_{\odot}$

The molecular gas resolved by ALMA in the low-metallicity dwarf merging galaxy Haro 11

The physical mechanisms for starburst or quenching in less massive ($M_* < 10^{10} M_{\odot}$) galaxies are unclear. The merger is one of the inescapable processes referred to as both starburst and quenching in massive galaxies. However, the effects of the merger on star formation in dwarf galaxies and their evolution results are still uncertain. We aim to explore how to trigger and quench star formation in dwarf galaxies by studying the metal-poor gas-rich dwarf mergers based on the multi-band observations at a spatial resolution of $\sim$ 460 pc. We use the archival data of ALMA (band 3, 8) and VLT/MUSE to map CO($J=$1-0), [CI]($^3$P$_1 - ^3$P$_0$), and H$\alpha$ emission in one of the most extreme starburst merging dwarf galaxies, Haro 11. We find the molecular gas is assembled around the central two star-forming regions. The molecular/ionized gas and stellar components show complex kinematics, indicating that the gas is probably at a combined stage of collision of clouds and feedback from star formation. The peak location and distribution of [CI](1-0) strongly resemble the CO(1-0) emission, meaning that it might trace the same molecular gas as CO in such a dwarf merger starburst galaxy. The enhancement of line ratios ($\sim 0.5$) of [CI]/CO around knot C is probably generated by the dissociation of CO molecules by cosmic rays and far-ultraviolet photons. Globally, Haro 11 and its star-forming regions share similar SFEs as the high-$z$ starburst galaxies or the clumps in nearby (U)LIRGs. Given the high SFE, sSFR, small stellar mass, low metallicity, and deficient HI gas, Haro 11 could be an analog of high-$z$ dwarf starburst and the potential progenitor of the nearby less massive elliptical galaxies. The significantly smaller turbulent pressure and viral parameter will probably trigger the intense starbursts. We also predict that it will quench at $M_* < 8.5 \times 10^9 M_{\odot}$